1. A single-cell atlas of mouse olfactory bulb chromatin accessibility
- Author
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Qikai Feng, Jiaying Qiu, Haoyu Wang, Rong Xiang, Zhen Huang, Xingliang Zhang, Xiangning Ding, Peiwen Ding, Feiyue Wang, Mingyue Wang, Jiankang Li, Yin Chen, Shengping Tang, Gen Tang, Shiyou Wang, Xiaoling Wang, and Zaoxu Xu
- Subjects
Epigenomics ,Cell ,Olfaction ,Computational biology ,Biology ,Epigenesis, Genetic ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Genetics ,medicine ,Animals ,Cluster Analysis ,Cell Lineage ,Gene Regulatory Networks ,Regulatory Elements, Transcriptional ,Epigenetics ,Nucleotide Motifs ,Molecular Biology ,Transcription factor ,Gene ,030304 developmental biology ,0303 health sciences ,RNA ,Olfactory Bulb ,Chromatin ,Olfactory bulb ,medicine.anatomical_structure ,Viruses ,Single-Cell Analysis ,030217 neurology & neurosurgery ,Transcription Factors - Abstract
Olfaction, the sense of smell, is a fundamental trait crucial to many species. The olfactory bulb (OB) plays pivotal roles in processing and transmitting odor information from the environment to the brain. The cellular heterogeneity of the mouse OB has been studied using single-cell RNA sequencing. However, the epigenetic landscape of the mOB remains mostly unexplored. Herein, we apply single-cell assay for transposase-accessible chromatin sequencing to profile the genome-wide chromatin accessibility of 9,549 single cells from the mOB. Based on single-cell epigenetic signatures, mOB cells are classified into 21 clusters corresponding to 11 cell types. We identify distinct sets of putative regulatory elements specific to each cell cluster from which putative target genes and enriched potential functions are inferred. In addition, the transcription factor motifs enriched in each cell cluster are determined to indicate the developmental fate of each cell lineage. Our study provides a valuable epigenetic data set for the mOB at single-cell resolution, and the results can enhance our understanding of regulatory circuits and the therapeutic capacity of the OB at the single-cell level.
- Published
- 2021