1. Degradation of 3,5,6-trichloro-2-pyridinol by a microbial consortium in dryland soil with anaerobic incubation
- Author
-
Mingzhu Shao, Huiming Huang, Shenghui Wang, Chen Zhang, Xueting Cao, Zhifeng Song, and Zhiwei Lv
- Subjects
Environmental Engineering ,Pyridones ,Aerobic bacteria ,Microbial Consortia ,Microbial metabolism ,Bioengineering ,010501 environmental sciences ,01 natural sciences ,Microbiology ,03 medical and health sciences ,Environmental Chemistry ,Anaerobiosis ,Soil Microbiology ,0105 earth and related environmental sciences ,0303 health sciences ,biology ,030306 microbiology ,Chemistry ,Biodegradation ,Microbial consortium ,biology.organism_classification ,Pollution ,Biodegradation, Environmental ,Delftia ,Environmental chemistry ,Cupriavidus ,Anaerobic exercise ,Bacteria - Abstract
Biodegradation of 3,5,6-trichloro-2-pyridinol (TCP) in drylands is an important biological process of detoxification. Flooding in drylands can result in the formation of anaerobic habitats. However, little is known about the microbial metabolism of TCP in dryland soil under anaerobic conditions. Here, chlorpyrifos-contaminated dryland soil was incubated to enrich the TCP-degrading microbial consortium under anaerobic conditions. Chloridion and CO2 were released with TCP degradation, and the enrichment cultures of dryland soil could metabolize 97% of TCP (100 mg/L) within 20 h. Both reductive and hydrolysis dechlorination mechanisms were involved in TCP biodegradation under anaerobic conditions. Bacterial taxonomic analysis revealed that the aerobic TCP-degrading bacteria Ochrobactrum and dechlorination bacteria Delftia were the dominant genera. Anaerobic and facultative bacteria; i.e., Bacteroides, Bacillus, and Cupriavidus had lower relative abundances, but they were significantly enriched following treatment with TCP. These results indicate that the enrichment cultures of dryland soil dominated by aerobic bacteria could dechlorinate and degrade TCP under anaerobic conditions.
- Published
- 2019