1. Presence of an EML4-ALK Gene Fusion Detected by Microfluidic Chip DNA Hybridization
- Author
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Paul C.H. Li, Christopher Oberc, and Montek Boparai
- Subjects
0301 basic medicine ,Oncogene Proteins, Fusion ,Applied Microbiology and Biotechnology ,Biochemistry ,Analytical Chemistry ,03 medical and health sciences ,0302 clinical medicine ,Lab-On-A-Chip Devices ,Sense (molecular biology) ,Anaplastic lymphoma kinase ,Humans ,Biochip ,Molecular Biology ,Gene ,Fusion ,Oncogene ,Base Sequence ,Chemistry ,Oligonucleotide ,DNA–DNA hybridization ,Organic Chemistry ,Nucleic Acid Hybridization ,General Medicine ,Molecular biology ,3. Good health ,030104 developmental biology ,030220 oncology & carcinogenesis ,Biotechnology - Abstract
Non-small cell lung cancer (NSCLC) accounts for ∼80-85% of all lung cancer cases, and the EML4-ALK fusion oncogene is a well-known contributor to NSCLC cases. Expensive methods such as FISH, IHC, and NGS have been used to detect the EML4-ALK fusion oncogene. Here, a cost-effective and facile method of detecting and differentiating an EML4-ALK fusion oncogene from the wild-type gene has been accomplished by DNA hybridization using the microfluidic biochip. First, oligonucleotide probes were confirmed for successful detection of immobilized sense strands. Second, capture of the sense PCR product strands (fusion and WT) and their subsequent detection and differentiation were accomplished. Our proof-of-concept study shows the ability to detect 1% fusion products, among WT ones.
- Published
- 2021