1. A tailored next‐generation sequencing panel identified distinct subtypes of wildtype IDH and TERT promoter glioblastomas
- Author
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Seiya Yokoyama, Hiroshi Hosoyama, Hiroyuki Uchida, Tomoko Hanada, Kei Matsuo, Akihisa Sakamoto, Akihide Tanimoto, Toshiaki Akahane, Hajime Yonezawa, Nayuta Higa, Koji Yoshimoto, Taiji Hamada, Masanori Yonenaga, Shingo Fujio, Tomoko Takajo, Mari Kirishima, and Tsubasa Hiraki
- Subjects
Adult ,Male ,0301 basic medicine ,Cancer Research ,Receptor, Platelet-Derived Growth Factor alpha ,IDH1 ,next‐generation sequencing ,PDGFRA ,1p/19q Codeletion ,Gene mutation ,Biology ,03 medical and health sciences ,0302 clinical medicine ,CDKN2A ,Glioma ,medicine ,Humans ,PTEN ,Oligodendroglial Tumor ,PDGFRA alterations ,Promoter Regions, Genetic ,Genetics, Genomics, and Proteomics ,Telomerase ,neoplasms ,Aged ,Aged, 80 and over ,1p/19q codeletion ,glioblastoma ,High-Throughput Nucleotide Sequencing ,General Medicine ,Middle Aged ,medicine.disease ,Isocitrate Dehydrogenase ,Neoplasm Proteins ,nervous system diseases ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,biology.protein ,Cancer research ,TERT promoter ,Original Article ,Female - Abstract
Central nervous system tumors are classified based on an integrated diagnosis combining histology and molecular characteristics, including IDH1/2 and H3‐K27M mutations, as well as 1p/19q codeletion. Here, we aimed to develop and assess the feasibility of a glioma‐tailored 48‐gene next‐generation sequencing (NGS) panel for integrated glioma diagnosis. We designed a glioma‐tailored 48‐gene NGS panel for detecting 1p/19q codeletion and mutations in IDH1/2, TP53, PTEN, PDGFRA, NF1, RB1, CDKN2A/B, CDK4, and the TERT promoter (TERTp). We analyzed 106 glioma patients (grade II: 19 cases, grade III: 23 cases, grade IV: 64 cases) using this system. The 1p/19q codeletion was detected precisely in oligodendroglial tumors using our NGS panel. In a cohort of 64 grade Ⅳ gliomas, we identified 56 IDH‐wildtype glioblastomas. Within these IDH‐wildtype glioblastomas, 33 samples (58.9%) showed a mutation in TERTp. Notably, PDGFRA mutations and their amplification were more commonly seen in TERTp‐wildtype glioblastomas (43%) than in TERTp‐mutant glioblastomas (6%) (P = .001). Hierarchical molecular classification of IDH‐wildtype glioblastomas revealed 3 distinct groups of IDH‐wildtype glioblastomas. One major cluster was characterized by mutations in PDGFRA, amplification of CDK4 and PDGFRA, homozygous deletion of CDKN2A/B, and absence of TERTp mutations. This cluster was significantly associated with older age (P = .021), higher Ki‐67 score (P = .007), poor prognosis (P = .012), and a periventricular tumor location. We report the development of a glioma‐tailored NGS panel for detecting 1p/19q codeletion and driver gene mutations on a single platform. Our panel identified distinct subtypes of IDH‐ and TERTp‐wildtype glioblastomas with frequent PDGFRA alterations., A glioma‐tailored 48‐gene next‐generation sequencing panel identified distinct subtypes of wildtype IDH and TERT promoter glioblastomas with PDGFRA alterations.
- Published
- 2020
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