1. Kidney intercalated cells are phagocytic and acidify internalized uropathogenic Escherichia coli
- Author
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Vijay Saxena, David S. Hains, Hongyu Gao, Malgorzata M. Kamocka, Samuel Arregui, Michael P. Hutchens, Xiaoling Xuei, Takashi Hato, Andrew L. Schwaderer, Patrick C. McGuire, and Amy Zollman
- Subjects
0301 basic medicine ,Cell biology ,Vacuolar Proton-Translocating ATPases ,Phagocytosis ,Science ,education ,030232 urology & nephrology ,General Physics and Astronomy ,Mice, Transgenic ,medicine.disease_cause ,Kidney ,General Biochemistry, Genetics and Molecular Biology ,Article ,Green fluorescent protein ,Biological pathway ,03 medical and health sciences ,0302 clinical medicine ,Phagosome maturation ,medicine ,Animals ,Humans ,Uropathogenic Escherichia coli ,Intercalated Cell ,Kidney Tubules, Collecting ,Escherichia coli ,Cells, Cultured ,health care economics and organizations ,Phagocytes ,Multidisciplinary ,Microscopy, Confocal ,biology ,Chemistry ,Gene Expression Profiling ,Epithelial Cells ,General Chemistry ,biology.organism_classification ,Mice, Inbred C57BL ,030104 developmental biology ,Mechanisms of disease ,Single-Cell Analysis ,Acids ,Homeostasis ,Bacteria - Abstract
Kidney intercalated cells are involved in acid-base homeostasis via vacuolar ATPase expression. Here we report six human intercalated cell subtypes, including hybrid principal-intercalated cells identified from single cell transcriptomics. Phagosome maturation is a biological process that increases in biological pathway analysis rank following exposure to uropathogenic Escherichia coli in two of the intercalated cell subtypes. Real time confocal microscopy visualization of murine renal tubules perfused with green fluorescent protein expressing Escherichia coli or pHrodo Green E. coli BioParticles demonstrates that intercalated cells actively phagocytose bacteria then acidify phagolysosomes. Additionally, intercalated cells have increased vacuolar ATPase expression following in vivo experimental UTI. Taken together, intercalated cells exhibit a transcriptional response conducive to the kidney’s defense, engulf bacteria and acidify the internalized bacteria. Intercalated cells represent an epithelial cell with characteristics of professional phagocytes like macrophages., Kidney intercalated cells are involved in acid-base homeostasis in the kidneys. Here, the authors use single cell transcriptomics and find that interalated cells exhibit a transcriptional response conducive to defense and can engulf and acidify internalized bacteria, similarly to professional phagocytes.
- Published
- 2021