1. Bacteria-derived long chain fatty acid exhibits anti-inflammatory properties in colitis
- Author
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Gilles Dietrich, Catherine Blanpied, Filip Van Immerseel, Claude Knauf, Pauline Le Faouder, Julien Pujo, Camille Petitfils, Sarah Maurel, Gaelle Payros, Teresa Pérez-Berezo, Stéphane Chavanas, Patrice D. Cani, Matthias Van Hul, Venessa Eeckhaut, Justine Bertrand-Michel, Eric Oswald, Frédérick Barreau, Nicolas Cenac, Institut de Recherche en Santé Digestive (IRSD ), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), McMaster University [Hamilton, Ontario], Institut des Maladies Métaboliques et Cardiovasculaires (I2MC), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Universiteit Gent = Ghent University [Belgium] (UGENT), Université Catholique de Louvain = Catholic University of Louvain (UCL), Centre de Physiopathologie Toulouse Purpan (CPTP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), FNRS : WELBIO-CR-2019C-02R, FNRS : 'The Excellence Of Science: EOS 30770923', Funds Baillet Latour (Grant for Medical Research 2015), ANR-18-CE14-0039,LiBacPain,Les lipopeptides produits par le microbiote : de l'hypersensibilité à la thérapie dans le syndrome de l'intestin irritable(2018), ANR-18-CE14-0007,ENDIABAC,LE SYSTEME NERVEUX ENTERIQUE COMME CIBLE POUR TRAITER LE DIABETE DE TYPE 2 : ROLES DES LIPIDES BIOACTIFS BACTERIENS(2018), and UCL - SSS/LDRI - Louvain Drug Research Institute
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Cell Membrane Permeability ,Chemokine CXCL1 ,medicine.medical_treatment ,Interleukin-1beta ,Gene Expression ,Oligosaccharides ,Peroxisome proliferator-activated receptor ,Pancreatitis-Associated Proteins ,Gut flora ,medicine.disease_cause ,Mass Spectrometry ,law.invention ,Mice ,Peyer's Patches ,Probiotic ,0302 clinical medicine ,law ,Intestinal Mucosa ,chemistry.chemical_classification ,0303 health sciences ,biology ,Dextran Sulfate ,Gastroenterology ,Colitis ,Lipids ,Enteric bacterial microflora ,Experimental colitis ,3. Good health ,enteric bacterial microflora ,030211 gastroenterology & hepatology ,lipids (amino acids, peptides, and proteins) ,Long chain fatty acid ,experimental colitis ,Firmicutes ,Permeability ,Microbiology ,lipids ,03 medical and health sciences ,Stearates ,Escherichia coli ,medicine ,[SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] ,Animals ,Humans ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,030304 developmental biology ,Bacteroidetes ,Probiotics ,Prebiotic ,Epithelial Cells ,medicine.disease ,biology.organism_classification ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Gastrointestinal Microbiome ,PPAR gamma ,Prebiotics ,chemistry ,probiotics ,Zonula Occludens-1 Protein ,Caco-2 Cells ,Bacteria ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology - Abstract
ObjectiveData from clinical research suggest that certain probiotic bacterial strains have the potential to modulate colonic inflammation. Nonetheless, these data differ between studies due to the probiotic bacterial strains used and the poor knowledge of their mechanisms of action.DesignBy mass-spectrometry, we identified and quantified free long chain fatty acids (LCFAs) in probiotics and assessed the effect of one of them in mouse colitis.ResultsAmong all the LCFAs quantified by mass spectrometry in Escherichia coli Nissle 1917 (EcN), a probiotic used for the treatment of multiple intestinal disorders, the concentration of 3-hydroxyoctadecaenoic acid (C18-3OH) was increased in EcN compared with other E. coli strains tested. Oral administration of C18-3OH decreased colitis induced by dextran sulfate sodium in mice. To determine whether other bacteria composing the microbiota are able to produce C18-3OH, we targeted the gut microbiota of mice with prebiotic fructooligosaccharides (FOS). The anti-inflammatory properties of FOS were associated with an increase in colonic C18-3OH concentration. Microbiota analyses revealed that the concentration of C18-3OH was correlated with an increase in the abundance in Allobaculum, Holdemanella and Parabacteroides. In culture, Holdemanella biformis produced high concentration of C18-3OH. Finally, using TR-FRET binding assay and gene expression analysis, we demonstrated that the C18-3OH is an agonist of peroxisome proliferator activated receptor gamma.ConclusionThe production of C18-3OH by bacteria could be one of the mechanisms implicated in the anti-inflammatory properties of probiotics. The production of LCFA-3OH by bacteria could be implicated in the microbiota/host interactions.
- Published
- 2021