1. Diagnosis of severe community-acquired pneumonia caused by Acinetobacter baumannii through next-generation sequencing: a case report.
- Author
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Xu A, Zhu H, Gao B, Weng H, Ding Z, Li M, Weng X, and He G
- Subjects
- Acinetobacter Infections drug therapy, Acinetobacter Infections microbiology, Acute Kidney Injury complications, Aged, Anti-Bacterial Agents therapeutic use, China, Community-Acquired Infections blood, Community-Acquired Infections drug therapy, Cross Infection, Dyspnea complications, Fever complications, Hospitalization, Humans, Male, Microbial Sensitivity Tests, Pneumonia, Bacterial blood, Pneumonia, Bacterial drug therapy, Respiratory Insufficiency complications, Shock, Septic complications, Sputum microbiology, Treatment Outcome, Acinetobacter Infections diagnosis, Acinetobacter baumannii genetics, Community-Acquired Infections diagnosis, High-Throughput Nucleotide Sequencing, Pneumonia, Bacterial diagnosis
- Abstract
Background: Acinetobacter baumannii is a gram-negative aerobic bacillus that is commonly causes of hospital-acquired infections. Community-acquired pneumonia caused by Acinetobacter baumannii (CAP-Ab) is rare but fatal if diagnosis and treatment are delayed. Conventional culture of clinical specimens is the main method for clinical diagnosis of A. baumannii infections which may suffer from limited positive rate and is time consuming. Timely and precise diagnosis of CAP-Ab remains challenging., Case Presentation: A 66-year-old man with 24 h history of acute fever and dyspnea was admitted to our hospital. He was diagnosed as severe community acquired pneumonia (CAP), septic shock, respiratory failure and acute kidney injury. Next-generation sequencing (NGS) was performed on the patient's sputum and blood, which identified numerous A. baumannii nucleotide sequences in the sample of sputum and led to the rapid diagnosis and treatment of community acquired pneumonia caused by A. baumannii. This result was confirmed by subsequent sputum culture., Conclusions: This case described that the successful application of the next generation sequencing assisting the speedy diagnosis of A. baumannii infection provides a new idea for the timely diagnosis of CAP-Ab and highlights that NGS is a promising tool in rapid etiological diagnosis of acute and severe infectious diseases.
- Published
- 2020
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