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Start Over Descriptor "crabtree-negative yeast" Topic alcohol dehydrogenase
2 results on '"crabtree-negative yeast"'

1. Identification of major ADH genes in ethanol metabolism of Pichia pastoris.

Author

Karaoğlan M, Erden-Karaoğlan F, Yılmaz S, and İnan M

Subjects
Alcohol Oxidoreductases genetics, Culture Media, Escherichia coli genetics, Fungal Proteins genetics, Fungal Proteins metabolism, Gene Expression Regulation, Fungal, Glucose metabolism, Glycerol metabolism, Methanol metabolism, Pichia growth & development, Promoter Regions, Genetic, Recombinant Proteins metabolism, Sequence Analysis, Protein, Sequence Homology, Amino Acid, Alcohol Dehydrogenase genetics, Ethanol metabolism, Genes, Fungal genetics, Pichia enzymology, Pichia genetics, Pichia metabolism
Abstract

The methylotrophic yeast Pichia pastoris (syn. Komagataella phaffii) is a successful host widely used in recombinant protein production. The widespread use of a methanol-regulated alcohol oxidase 1 (AOX1) promoter for recombinant protein production has directed studies particularly about methanol metabolism in this yeast. Although there is comprehensive knowledge about methanol metabolism, there are other mechanisms in P. pastoris that have not been investigated yet, such as ethanol metabolism. The gene responsible for the consumption of ethanol ADH2 (XM_002491337, known as ADH3) was identified and characterized in our previous study. In this study, the ADH genes (XM_002489969, XM_002491163, XM_002493969) in P. pastoris genome were investigated to determine their roles in ethanol production by gene disruption analysis. We report that the ADH900 (XM_002491163) is the main gene responsible for ethanol production in P. pastoris. The ADH2 gene, previously identified as the only gene responsible for ethanol consumption, also plays a minor role in ethanol production in the absence of the ADH900 gene. The investigation of the carbon source regulation mechanism has also revealed that the ADH2 gene exhibit similar expression behaviours with ADH900 on glucose, glycerol, and methanol, however, it is strongly induced by ethanol., (© 2019 John Wiley & Sons, Ltd.)

Published
2020
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2. Identification Of Major Adh Genes In Ethanol Metabolism Of Pichia Pastoris

Author

Mehmet Inan, Fidan Erden-Karaoğlan, Mert Karaoglan, Semiramis Yılmaz, and EBYÜ, Mühendislik Fakültesi

Subjects
0106 biological sciences, Glycerol, crabtree-negative yeast, Genes, Fungal, Bioengineering, Biology, 01 natural sciences, Applied Microbiology and Biotechnology, Biochemistry, Pichia, Pichia pastoris, ethanol metabolism, Fungal Proteins, 03 medical and health sciences, chemistry.chemical_compound, Sequence Analysis, Protein, 010608 biotechnology, Gene Expression Regulation, Fungal, ethanol production, Genetics, Escherichia coli, Ethanol fuel, Ethanol metabolism, Promoter Regions, Genetic, Gene, 030304 developmental biology, Alcohol dehydrogenase, 0303 health sciences, Ethanol, Sequence Homology, Amino Acid, Methanol, Alcohol Dehydrogenase, biology.organism_classification, Yeast, Recombinant Proteins, Alcohol oxidase, Culture Media, Alcohol Oxidoreductases, Glucose, chemistry, biology.protein, Biotechnology
Abstract

The methylotrophic yeast Pichia pastoris (syn. Komagataella phaffii) is a successful host widely used in recombinant protein production. The widespread use of a methanol-regulated alcohol oxidase 1 (AOX1) promoter for recombinant protein production has directed studies particularly about methanol metabolism in this yeast. Although there is comprehensive knowledge about methanol metabolism, there are other mechanisms in P. pastoris that have not been investigated yet, such as ethanol metabolism. The gene responsible for the consumption of ethanol ADH2 (XM_002491337, known as ADH3) was identified and characterized in our previous study. In this study, the ADH genes (XM_002489969, XM_002491163, XM_002493969) in P. pastoris genome were investigated to determine their roles in ethanol production by gene disruption analysis. We report that the ADH900 (XM_002491163) is the main gene responsible for ethanol production in P. pastoris. The ADH2 gene, previously identified as the only gene responsible for ethanol consumption, also plays a minor role in ethanol production in the absence of the ADH900 gene. The investigation of the carbon source regulation mechanism has also revealed that the ADH2 gene exhibit similar expression behaviours with ADH900 on glucose, glycerol, and methanol, however, it is strongly induced by ethanol.

Published
2020

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