1. Differentiated metabolomic profiling reveals plasma amino acid signatures for primary glomerular disease.
- Author
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Wang J, Zhou C, Lu L, Wang S, Zhang Q, and Liu Z
- Subjects
- Humans, Female, Male, Adult, Middle Aged, Glomerulosclerosis, Focal Segmental blood, Glomerulosclerosis, Focal Segmental diagnosis, Nephrosis, Lipoid blood, Nephrosis, Lipoid diagnosis, Glomerulonephritis, Membranous blood, Glomerulonephritis, Membranous diagnosis, Tandem Mass Spectrometry, Chromatography, High Pressure Liquid, Glomerulonephritis, IGA blood, Glomerulonephritis, IGA diagnosis, Kidney Glomerulus metabolism, Kidney Glomerulus pathology, Amino Acids blood, Metabolomics methods, Biomarkers blood
- Abstract
Primary glomerular disease (PGD) is an idiopathic cause of renal glomerular lesions that is characterized by proteinuria or hematuria and is the leading cause of chronic kidney disease (CKD). The identification of circulating biomarkers for the diagnosis of PGD requires a thorough understanding of the metabolic defects involved. In this study, ultra-high performance liquid chromatography-tandem mass spectrometry was performed to characterize the amino acid (AA) profiles of patients with pathologically diagnosed PGD, including minimal change disease (MCD), focal segmental glomerular sclerosis (FSGS), membranous nephropathy, and immunoglobulin A nephropathy. The plasma concentrations of asparagine and ornithine were low, and that of aspartic acid was high, in patients with all the pathologic types of PGD, compared to healthy controls. Two distinct diagnostic models were generated using the differential plasma AA profiles using logistic regression and receiver operating characteristic analyses, with areas under the curves of 1.000 and accuracies up to 100.0% in patients with MCD and FSGS. In conclusion, the progression of PGD is associated with alterations in AA profiles, The present findings provide a theoretical basis for the use of AAs as a non-invasive, real-time, rapid, and simple biomarker for the diagnosis of various pathologic types of PGD., (© 2024. The Author(s).)
- Published
- 2024
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