1. Synergistic antipseudomonal effects of synthetic peptide AMP38 and carbapenems
- Author
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Héctor Rudilla, Francesc Rabanal, Teresa Vinuesa, Miguel Viñas, Ester Fusté, Yolanda Cajal, and Universitat de Barcelona
- Subjects
0301 basic medicine ,Imipenem ,030106 microbiology ,Antimicrobial peptides ,Pharmaceutical Science ,Peptide ,Antibiòtics ,Biology ,medicine.disease_cause ,Article ,biofilm eradication ,Analytical Chemistry ,Microbiology ,lcsh:QD241-441 ,antimicrobial peptides ,03 medical and health sciences ,lcsh:Organic chemistry ,Antibiotics ,synergism ,Pseudomonas ,Drug Discovery ,medicine ,polycyclic compounds ,Physical and Theoretical Chemistry ,chemistry.chemical_classification ,Pseudomonas aeruginosa ,Organic Chemistry ,Biofilm ,biochemical phenomena, metabolism, and nutrition ,Antimicrobial ,biology.organism_classification ,bacterial infections and mycoses ,Síntesi de pèptids ,030104 developmental biology ,Peptide synthesis ,Carbapenems ,chemistry ,Chemistry (miscellaneous) ,Biofilms ,Colistin ,Molecular Medicine ,Pèptids ,Peptides ,Antimicrobial Cationic Peptides ,medicine.drug - Abstract
The aim was to explore the antimicrobial activity of a synthetic peptide (AMP38) and its synergy with imipenem against imipenem-resistant Pseudomonas aeruginosa. The main mechanism of imipenem resistance is the loss or alteration of protein OprD. Time-kill and minimal biofilm eradication concentration (MBEC) determinations were carried out by using clinical imipenem-resistant strains. AMP38 was markedly synergistic with imipenem when determined in imipenem-resistant P. aeruginosa. MBEC obtained for the combination of AMP38 and imipenem was of 62.5 μg/mL, whereas the MBEC of each antimicrobial separately was 500 μg/mL. AMP38 should be regarded as a promising antimicrobial to fight MDR P. aeruginosa infections. Moreover, killing effect and antibiofilm activity of AMP38 plus imipenem was much higher than that of colistin plus imipenem.
- Published
- 2016