1,194 results on '"Angiogenèse"'
Search Results
2. Impacto de la actividad física en la homeostasis de factores pro yanti angiogénicos placentarios para un embarazo seguro: Una Revisión Sistemática.
- Author
-
Vela, María Fernández
- Subjects
PHYSICAL activity ,VASCULAR endothelial growth factors ,PREGNANT women ,HEMATOPOIESIS ,BLOOD vessels - Abstract
Copyright of Revista Andaluza de Medicina del Deporte is the property of Centro Andaluza de Medicina del Deporte and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
- Published
- 2024
- Full Text
- View/download PDF
Catalog
3. Diagnostik und Therapie der fetalen und neonatalen Alloimmunthrombozytopenie
- Author
-
Gembruch, Ulrich, Geipel, Annegret, von Kaisenberg, Constantin, editor, Klaritsch, Philipp, editor, and Hösli-Krais, Irene, editor
- Published
- 2024
- Full Text
- View/download PDF
4. Retinales Pigmentepithel bei proliferativen Erkrankungen
- Author
-
Dik, Willem A., Bastiaans, Jeroen, van Meurs, Jan C., Klettner, Alexa Karina, editor, and Dithmar, Stefan, editor
- Published
- 2024
- Full Text
- View/download PDF
5. Antiangiogenic activity of photobiomodulation in experimental model using chorioallantoic embryonic membrane of chicken eggs.
- Author
-
Nunes Dourado, Lays Fernanda, Camargo Siqueira, Rubens, Paula Alves, Ana, Brandão de Paiva, Mayara Rodrigues, Agero, Ubirajara, and Cunha Junior, Armando da Silva
- Subjects
CHORIOALLANTOIS ,RED light ,EGGS ,PHOTOBIOMODULATION therapy ,PHOTOTHERAPY ,NEOVASCULARIZATION inhibitors ,MACULAR degeneration - Abstract
Copyright of Arquivos Brasileiros de Oftalmologia is the property of Arquivos Brasileiros de Oftalmologia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
- Published
- 2024
- Full Text
- View/download PDF
6. THE EFFECTS OF NEGATIVE PRESSURE WOUND THERAPY ON VEGF AND ANGIOGENESIS IN DEEP DERMAL BURN INJURY: AN EXPERIMENTAL STUDY.
- Author
-
M. R., Seswandhana, I. D., Kurniawan, S. L., Anwar, H. M. A., Humani, G. C., Gabriela, I., Dachlan, Y. W., Wirohadidjojo, and I., Aryandono
- Subjects
- *
NEGATIVE-pressure wound therapy , *NEOVASCULARIZATION , *SILVER sulfadiazine , *YORKSHIRE swine , *WOUND healing , *IMMUNOSTAINING - Abstract
Burn injuries are the fourth most common type of trauma worldwide, after traffic injuries, falls and interpersonal violence. Vascular endothelial growth factor (VEGF) is one of the most critical proangiogenic factors. Failure in angiogenesis is often associated with chronic, non-healing wounds. This study aimed to compare the effect ofsterile gauze with normalsaline (NaCl) 0.9%, intermittent negative pressure wound therapy (NPWT), continuous NPWT, and silver sulfadiazine dressing on increasing VEGF and angiogenesis in deep dermal burn injury. This experimental laboratory study involved six Yorkshire pigs. Twenty burns were made on each pig’s flank and dorsum areas, which were divided into four treatment groups: sterile gauze with NaCl 0.9%, intermittent NPWT, continuous NPWT, and silver sulfadiazine dressing. Skin biopsies were done on days 1, 3, 7, 14 and 21 to evaluate VEGF histoscore and mean microvascular density (MVD). We used immunohistochemical staining of VEGF-165 as VEGF’s protein marker and hematoxylin-eosin (HE) to count the MVD. There was a significant difference in mean VEGF histoscore on evaluation day 14, in which continuous NPWT had the highestscore compared to sterile gauze with NaCl 0.9%, intermittent NPWT, and silver sulfadiazine. The elevated VEGF histoscore could significantly increase the MVD. [ABSTRACT FROM AUTHOR] more...
- Published
- 2023
7. Kortikosteroidwirkung auf Sehnen- und Bindegewebsheilung.
- Author
-
Bloch, Wilhelm
- Abstract
Lokal-injizierte und systemisch verabreichte Kortikosteroide werden für die Behandlung von muskuloskelettalen Verletzungen im Sport genutzt. Es bestehen kritische Bewertungen des Nebenwirkungsprofils, der Komplikationen nach operativen Eingriffen und des Einflusses auf den Heilungsprozess. Dies wirft die Frage nach Mechanismen auf, die den Heilungs- und Regenerationsprozess von Sehnen und Bändern nach Kortikosteroidgabe beeinflussen. Kortikosteroide beeinflussen die Prozesse, die an der Heilung und Regeneration von Sehnen und Bändern beteiligt sind, auf verschiedenen Ebenen. Dazu gehören negative Effekte auf die Extrazellularmatrixzusammensetzung, Tenozyten- und Fibrozytenfunktion, sowie der Einfluss auf die Gefäßentwicklung während des Heilungsprozesses und die lokale initiale Entzündung, die für einen geregelten Wundheilungsprozess wesentlich sind. Insbesondere bei mechanisch stark belasteten Sehnen ist dies ein Risiko. Die epigenetischen Veränderungen durch Kortikosteroide in Bindegewebszellen können erklären, warum es zu längerfristigen Veränderungen der Sehnen und Bänder kommt. Um negative Einflüsse auf die Sehnen und Bandheilung zu verhindern, sind Zeitpunkt und Dosierung von Kortikosteroiden so zu wählen, dass der Heilungsprozess nicht negativ beeinflusst wird und im akuten Heilungsprozess zu vermeiden. Die mögliche Dopingproblematik auch nach lokalen Kortikosteroidinjektion sollte bei der Behandlung mit Kortikosteroiden berücksichtigt werden. Locally injected and systemic corticosteroids are used for the treatment of musculoskeletal in sports. There are critical reports of the side effect profile, the difficulties after surgery and the impact on the healing process. This raises the question of mechanisms that influence the healing and regeneration process of tendons and ligaments after corticosteroid administration. Corticosteroids affect the processes involved in healing and regeneration of tendons and ligaments at different levels. This includes negative effects on the extracellular matrix composition, tenocyte and fibrocyte function, as well as the influence on the angiogenesis during healing and the local initial inflammation. These processes are essential for a regulary wound healing. This is a risk, especially in the case of high mechanical load tendons. The epigenetic changes caused by corticosteroids in connective tissue cells can explain why there are long-term alterations in tendons and ligaments. In order to prevent negative influences on tendon and ligament healing, the timing and dosage of corticosteroids should be considered. The healing process shouldn't be negatively influenced and corticosteroids should be avoided in the acute healing process. [ABSTRACT FROM AUTHOR] more...
- Published
- 2023
- Full Text
- View/download PDF
8. Uso de células-tronco para cicatrização de pele em uma anta (Tapirus terrestres): Relato de caso
- Author
-
Filipe Carvalho Pereira, Matheus Werner Rêgo, Patrícia Furtado Malard, Victor Moraes Amorim, Gabriella Accardi Iglesias, and Hilana Dos Santos Sena Brunel
- Subjects
Biotecnologia ,Animais silvestres ,angiogênese ,lesões de pele ,células tronco mesenquimais ,Veterinary medicine ,SF600-1100 - Abstract
Uma anta (Tapirus terrestres) foi encontrada com uma grave lesão na cabeça, foi capturada e tratada durante 35 dias com limpeza local e pomada cicatrizante, porém com pouca evolução na cicatrização. O animal recebeu então a aplicação intradérmica de células-tronco mesenquimais (CTMs) heterólogas nas bordas da ferida. As CTMs foram isoladas do tecido adiposo de um equino doador saudável, cultivadas e caracterizadas de acordo com os critérios da International Society for Cell Therapy. A partir desse momento, a ferida passou a apresentar maior vascularização e presença de tecido de granulação, levando ao completo fechamento do local ao fim de 3 meses. Nesse momento a anta pôde ser reintroduzida na natureza. A terapia com CTMs mostrou-se eficaz no processo de aceleração da cicatrização da ferida do animal. more...
- Published
- 2023
- Full Text
- View/download PDF
9. Angiogênese tumoral
- Author
-
José Augusto Assumpção Crespo Ribeiro
- Subjects
Angiogênese ,Fator Angiogênico ,Neoplasia ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Este artigo faz uma revisão de literatura a respeito do processo de angiogênese tumoral. Apresenta o histórico das investigações, os conceitos, e a importância da angiogênese em relação ao crescimento neoplásico. Aborda o mecanismo de formação deste processo e a participação dos fatores angiogênicos. Por fim, mostra a aplicação destes conhecimentos na prática clínica em relação ao diagnóstico, terapêutica e prognóstico. more...
- Published
- 2023
- Full Text
- View/download PDF
10. Reparação do retalho axial dorsal em ratos tratados com óleo ozonizado
- Author
-
Aline Medeiros Nakamura, Marcelo Carrijo da Costa, Jorge Luis Álvarez Gómez, Felipe Noleto de Paiva, and Andrigo Barboza De Nardi
- Subjects
ozonioterapia ,cirurgia reconstrutiva ,angiogênese ,colágeno ,Veterinary medicine ,SF600-1100 - Abstract
O retalho de padrão axial representa uma importante opção na cirurgia reconstrutiva, sendo composto por uma artéria e uma veia cutânea direta em sua base, permitindo que uma grande área de pele possa constituir o retalho para recobrir a ferida. Entretanto, a técnica pode apresentar complicações, principalmente com a necrose devido ao comprometimento vascular. Nesse contexto, a ozonioterapia pode ser utilizada para acelerar o processo de cicatrização de feridas pouco vascularizadas e contaminadas. Com o presente estudo, objetivou-se avaliar os efeitos e o reparo do retalho axial dorsal em ratos Wistar, empregando tratamento com óleo ozonizado de alta peroxidação. Utilizaram-se 18 ratos, distribuídos em dois grupos com 9 animais, grupo GG (óleo de girassol comum) e grupo GO (óleo de girassol ozonizado). Durante o período experimental foram realizadas avaliações macroscópicas e microscópicas a fim de observar a cicatrização e a reparação da ferida cirúrgica. Os dados foram submetidos a análise estatística. O aspecto cosmético em GO foi significativamente melhor que GG, e a contagem de vasos sanguíneos, hemorragia e reepitelização foram significativamente maiores em GO quando comparado ao GG. A presença de necrose não diferiu entre os grupos. A média da área total dos retalhos entre os grupos diferiu estatisticamente, sendo maior nos retalhos em GO. Os resultados evidenciaram que a utilização do óleo ozonizado em retalhos de padrão axial resulta em aspecto favorável e melhor reepitelização. more...
- Published
- 2023
11. Role of genetic factors in recurrent miscarriages - A review.
- Author
-
Ndjapa-Ndamkou, Constant, Govender, Logie, and Chauke, Lawrence
- Subjects
UTERUS abnormalities ,EMBRYOS ,MISCARRIAGE ,ANTIPHOSPHOLIPID syndrome ,NEOVASCULARIZATION ,INFLAMMATION ,GENETIC testing ,DISEASE relapse ,METABOLIC disorders ,OXIDATIVE stress ,CHROMOSOME abnormalities - Abstract
Copyright of African Journal of Reproductive Health is the property of Women's Health & Action Research Centre and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
- Published
- 2022
- Full Text
- View/download PDF
12. Physiopathologie du psoriasis.
- Author
-
Seuve, Étienne, Eyraud, Alexia, and Desmoulière, Alexis
- Abstract
Le psoriasis est une dermatose inflammatoire chronique parfois affichante fréquemment rencontrée en officine. Les patients sont en quête de solutions. Les lésions psoriasiques sont en lien avec une physiopathologie complexe qu'il est important de connaître pour mieux comprendre cette pathologie. Psoriasis is a chronic inflammatory dermatosis that is frequently encountered in pharmacies. Patients are looking for solutions. Psoriatic lesions are linked to a complex physiopathology that it is important to know in order to better understand this pathology. [ABSTRACT FROM AUTHOR] more...
- Published
- 2022
- Full Text
- View/download PDF
13. Avaliação morfo-histológica e morfo-histométrica de feridas cutâneas tratadas com Sphagneticola trilobata (L.) Pruski em ratos
- Author
-
A.G.B. Leite, L.R.M. Estevão, C.J.F.L. Silva, J.L.S. Lima, A.A.V.C. Bulhões, E.B.A. Soares, and J. Evêncio-Neto
- Subjects
angiogênese ,cicatrização ,extrato ,fitoterápico ,pele ,Animal culture ,SF1-1100 - Abstract
RESUMO O objetivo deste trabalho foi avaliar macro e microscopicamente a atividade cicatrizante da Sphagneticola trilobata em feridas cutâneas induzidas em ratos, a partir da aplicação de creme contendo extrato hidroalcoólico bruto de folhas da planta. A análise fitoquímica apresentou terpenos e flavonoides como compostos majoritários. Sessenta ratos foram divididos em três grupos experimentais (n=20): grupo tratado (GT), grupo controle (GC) e grupo controle absoluto (GCA). Quatro feridas excisionais de 0,8cm de diâmetro foram realizadas no dorso dos animais, tratadas diariamente e avaliadas nos tempos três, sete, 14 e 21 dias de pós-operatório (PO) quanto à contração e à avaliação macroscópica, morfo-histológica e morfo-histométrica. Macroscopicamente, não houve diferença estatística na contração das feridas entre os grupos testados. Na avaliação morfológica e na morfométrica, o GT apresentou menor concentração de células inflamatórias, maior e melhor preenchimento do tecido de granulação pelas fibras colágenas e melhor vascularização das feridas. Não houve diferença entre o GC e o GCA. Conclui-se que o creme à base do extrato hidroalcoólico bruto das folhas de Sphagneticola trilobata contribui positivamente para o processo de cicatrização das feridas em pele de ratos. more...
- Published
- 2020
- Full Text
- View/download PDF
14. Hemangiossarcoma canino: revisão
- Author
-
Juliane Freitas, Lin Chieh Yi, and Gustavo Soares Forlani
- Subjects
angiogênese ,células endoteliais ,hemangioma ,oncologia veterinária ,Veterinary medicine ,SF600-1100 - Abstract
O hemangiossarcoma canino é uma neoplasia maligna de células endoteliais dos vasos sanguíneos, altamente metastáticos quando em sua forma visceral, podendo também se manifestar como forma primária cutânea. A espécie canina é descrita como sendo a mais afetada. Machos com idade entre dez e 12 anos são mais predispostos ao desenvolvimento do hemangiossarcoma (HSA). A manifestação clínica da doença é inespecífica e varia conforme a localização do sítio primário ou metástases. O diagnóstico definitivo ocorre através de histopatologia por biópsia do tumor. A terapia se dá por excisão cirúrgica e protocolos terapêuticos oncológicos. O prognóstico é considerado reservado, dependendo de qual fase foi diagnosticada a doença. Esta revisão de literatura tem por objetivo esclarecer e contribuir para o conhecimento da patologia, através da avaliação de órgãos e sistemas acometidos, sinais clínicos bem como apresentação de diagnósticos e tratamentos disponíveis. more...
- Published
- 2019
- Full Text
- View/download PDF
15. COVID-19: Auswirkungen auf Lunge und Herz.
- Author
-
Ackermann, Maximilian, Werlein, Christopher, Länger, Florian, Kühnel, Mark P., and Jonigk, Danny D.
- Abstract
Copyright of Der Pathologe is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
- Published
- 2021
- Full Text
- View/download PDF
16. CLÍNICA CIRÚRGICA DE PEQUENOS ANIMAIS - CONTROLE MICROBIOLÓGICO NA OBTENÇÃO DO PLASMA RICO EM PLAQUETAS (PRP).
- Author
-
SFRIZO, L. S., PAZZINI, J. M., FERREIRA, M. G. P. A., USCATEGUI, R. A. R., DE NARDI, A. B., and PIGATTO-DENARDI, C.
- Subjects
- *
SKIN grafting , *PLASTIC surgery , *BACTERIAL contamination , *SURGICAL site , *BACTERIAL colonies , *PLATELET-rich plasma - Abstract
Platelet-rich plasma is a product obtained by centrifuging blood, and due to its properties of stimulating angiogenesis, it is used in reconstructive surgery in order to avoid post-surgical complications. Blood collection is done aseptically to obtain platelet-rich plasma, but there is a possibility of bacterial contamination at the time of making it, thus causing complications to the patient. The objective was to emphasize the importance of rigor in the microbiological control of the product obtained, as well as to evaluate the possibility of bacterial contamination when making plateletrich plasma (PRP), before its use in the bed of skin grafts in rabbits. In the present study, only four samples of the sixty made had bacterial colonies, indicating that the platelet-rich plasma obtained aseptically is conducive and safe in reconstructive surgery. It is concluded that the rigor in microbiological control combined with adequate disinfection of the operating room and antisepsis of the operating field, as well as the aseptic preparation of the PRP, its use is indicated and safe, without causing risks of contamination of the surgical wound. [ABSTRACT FROM AUTHOR] more...
- Published
- 2020
- Full Text
- View/download PDF
17. ANÁLISE DO EFEITO DO TRATA MENTO COM NANOPART ÍCULAS DE PRATA (AgNO3) REVESTIDAS COM PECTINA SOBRE A ANGIOGÊNESE INDUZIDA PELAS CÉLULAS DO TUMOR DE EHRLICH EM MODELO DA MEMBRANA CORIOALANTOIDEA.
- Author
-
Malta Francese, Monique and Henrique Weckwerth, Paulo
- Subjects
- *
SILVER nanoparticles , *BLOOD grouping & crossmatching , *TUMOR growth , *EGGS , *MICROSCOPES , *NEOVASCULARIZATION , *COGNITIVE interference - Abstract
The interference of silver nanoparticles on angiogenesis related to tumor growth was evaluated. Scientific research was carried out by incubating 42 embryonated chicken eggs. After 24 hours of incubation, these same eggs were separated into six groups containing seven eggs each, for treatments with: Group 1: Saline; Group 2: Ehrlich's tumor (ET); Group 3: Silver nanoparticles; Group 4: Prednisolone; Group 5: Silver nanoparticles and Ehrlich's Tumor; Group 6: Prednisolone and Ehrlich's Tumor. After the total incubation time, the chorioallantoid membranes (MCAs) were removed, and analyzed using a light microscope and photographed. Group 1 showed a normal growth pattern and was used as a negative control; Group 2 showed an increase in the amount of blood vessels; group 3 showed low interference in embryonic angiogenesis and did not contribute to the development of the tumor; Group 4 demonstrated a decrease in the development of blood vessels; Group 5 indicated that silver nanoparticles, when associated with TE, do not favor tumor development and group 6 demonstrated that the drug prednisolone associated with TE, behaves as an excellent inhibitor of tumor neoangiogenesis. Through the technique performed, the possibility of using silver nanoparticles for the treatment of Ehrlich tumor cells is considered, however, confirmatory tests should be performed to study the relationship of the substance described to the tumor cells employed. [ABSTRACT FROM AUTHOR] more...
- Published
- 2020
18. A comprehensive analysis of the angiogenesis-related genes in glioblastoma multiforme vs. brain lower grade glioma.
- Author
-
SUT, Burcu Biterge
- Abstract
Copyright of Arquivos de Neuro-Psiquiatria is the property of Thieme Medical Publishing Inc. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
- Published
- 2020
- Full Text
- View/download PDF
19. Stammzellenangereicherte Fettinjektion in der ästhetischen, rekonstruktiven Brustchirurgie.
- Author
-
Tiryaki, K. Tunc and Canikyan, Serli
- Abstract
Copyright of Journal für Ästhetische Chirurgie is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
- Published
- 2019
- Full Text
- View/download PDF
20. In-vivo-Aktivierung von regenerativen Zellen im Fettgewebe für die Gesichtsrejuvenation.
- Author
-
Sandhofer, Matthias, Barsch, Martin, Wurzer, Christoph, Lindner, Carolin, and Priglinger, Eleni
- Abstract
Copyright of Journal für Ästhetische Chirurgie is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
- Published
- 2019
- Full Text
- View/download PDF
21. Advancements in diagnosis and treatment of Adenomyosis
- Subjects
transvaginal ultrasound ,transvaginale echoscopie ,angiogenesis ,uterus ,benign gynecology ,angiogenese ,goedaardige aandoeningen ,gynecology ,in vivo model ,adenomyose ,gyaecologie ,Adenomyosis - Abstract
In this thesis, we aimed to study how we can improve the diagnosing of adenomyosis by imaging the uterus in part I, especially using ultrasound and considering the assessment of the uterine junctional zone. In part II, we aimed to investigate the role of angiogenesis in the pathogenesis and clinical manifestation of adenomyosis and to explore whether this mechanism could be a potential treatment target. In chapter 2 (part I), we explored whether the terminology used to describe ultrasound images of adenomyosis needed to be better defined in a modified Delphi study. There was consensus on the need to distinguish between direct and indirect features of adenomyosis. Direct features indicate the presence of ectopic endometrium in the myometrium, while indirect features reflecting changes in the myometrium secondary to presence of endometrial tissue in the myometrium. In chapter 3 (Part I), a comprehensive literature review was conducted to investigate the accordance on the definition of the junctional zone across different diagnostic approaches and examine how the imaging findings can be linked to histological findings. The conclusion in this review was that on histology, gradual tissue changes distinguish the junctional zone from the middle and outer myometrium. The thickness of the junctional zone was reported larger on MRI than on TVUS images. Clinicians should be aware that findings on MRI cannot readily be extrapolated to ultrasound. The focus of the second part of this thesis was on the role of angiogenesis in adenomyosis. We hypothesized that the level of angiogenesis is increased in the ectopic and eutopic endometrium of patients with adenomyosis in comparison with patients without adenomyosis. In chapter 4 we performed a systematic search and found an increased mean vascular density (MVD) and angiogenic properties in the ectopic and eutopic endometrium compared to control endometrium. Although the association has not sufficiently been studied yet, it is likely that increased angiogenesis leads to fragile and more permeable vessels resulting in adenomyosis-related AUB and possibly subfertility. Since VEGF stimulates both angiogenesis and lymph-angiogenesis, the presence of lymph vessel angiogenesis was investigated in a retrospective matched case-control study described in chapter 5. The blood and lymph vessel densities were higher in the ectopic and eutopic endometrium than in control endometrium in patients that were in the proliferative phase of the menstrual cycle. The results of this study are evidence for the presence of increased angiogenesis and lymph angiogenesis in the (ectopic) endometrium of adenomyosis patients versus controls. In chapter 6, we investigated the angiogenic potential of endometrial and myometrial biopsies collected in a prospective study from patients with and without adenomyosis. The results confirm that adenomyosis tissue, in particular endometrium overlying adenomyosis lesions, has angiogenic potential and provides evidence for our hypothesis that angiogenesis plays a role in the pathogenesis of adenomyosis. Since the results of the studies described in the chapter above suggest a pivotal role for angiogenesis in the pathophysiology of adenomyosis, we investigated the effect of anti-angiogenic therapy in adenomyosis in an in vivo mouse model and described the results in chapter 7. This study showed that angiogenesis inhibition through oral axitinib treatment reduces the severity of adenomyosis. The hypothesis why this potential therapy is promising and might aid to reverse uterine aging is described in chapter 8. In our regard, anti-angiogenic therapy may provide therapeutic options. Finally, chapter 9 discusses the answers to the research questions of this thesis and provides clinical implications and suggestions for future research. more...
- Published
- 2023
- Full Text
- View/download PDF
22. Drop-on-demand bioprinting of HUVECs and capillary-like networks via laser-induced side transfer
- Author
-
Erfanian, Mahyar, Boutopoulos, Christos, and Larrivée, Bruno
- Subjects
laser-based bioprinting ,cellules endothéliales ,angiogenesis ,micro-vasculature ,biofabrication ,laser-induced side transfer (LIST) ,bioimpression par laser ,angiogenèse ,endothelial cells - Abstract
La fabrication de tissus biologiques a été largement étudiée pour ses applications dans la recherche, la transplantation d'organes et le dépistage de drogues. Bien que des tissus minces ou avasculaires aient été fabriqués avec succès auparavant, le maintien de la viabilité des tissus épais nécessite la présence d'un réseau capillaire tout au long de la construction pour permettre l'apport de nutriments et l'élimination des déchets cellulaires par le sang. En plus des cellules endothéliales, l'incorporation de types de cellules de soutien dans le réseau capillaire est nécessaire pour favoriser la survie et la maturation. Comparée à d'autres méthodes de biofabrication, la bioimpression est une technologie prometteuse qui permet la fabrication précise de motifs 3D complexes à haute résolution spatiale. Nous avons conçu de nouveau notre procédé technique de bio-impression laser nommé LIST (de l'anglais \textit{laser-induced side transfer}) dans laquelle la bioencre de la suspension cellulaire passe à travers un capillaire horizontal avec un orifice face à l'échafaudage. Lorsque le laser frappe la bioencre, une bulle se forme qui propulse une gouttelette à travers l'orifice. Nous avons mené une étude détaillée pour caractériser cette bio-impression technique et validé sa cytocompatibilité par l'évaluation de la viabilité de HUVECs imprimés grâce à LIST. Nous avons incorporé des fibroblastes et des péricytes dans nos échantillons et observé le recrutement progressif de ces cellules par les structures de type capillaire HUVEC imprimées sur Matrigel. Des images fluorescentes ont été analysées pour quantifier le recrutement de fibroblastes/péricytes au fil du temps., The fabrication of biological tissues in laboratory settings has been widely investigated for its applications in research, organ transplantation, and drug screening. Although several previous attempts to generate avascular or thin tissues have been successful, there remains the challenge to create thick functional tissues. Maintaining the viability of thick tissues requires the presence of a capillary network throughout the construct to allow the intake of nutrients and the discard of cellular waste through blood. In addition to endothelial cells, the incorporation of supporting cell types is necessary to promote survival, maturation, and acquire in vivo-like functionality. Compared to other biofabrication methods, bioprinting is a promising technology that enables the precise fabrication of complex 3D patterns at high spatial resolution. We have come up with a new configuration of our in-house laser-based bioprinting technique called laser-induced side transfer (LIST) in which the bioink passes through a horizontal glass capillary with an orifice facing the receiving substrate. When the laser beam causes bubble formation in the bioink, a liquid jet exits through the orifice that will eventually form a droplet. We have conducted a detailed study to characterize this bioprinting technique and validated its cytocompatibility through viability assessment of LIST-printed human umbilical vein endothelial cells (HUVECs). In an effort to generate physiological blood vessels, we incorporated fibroblasts and pericytes in our samples and observed the gradual recruitment of these cells by the printed HUVEC capillary-like structures on Matrigel. Fluorescent images were taken and analyzed to quantify the fibroblast/pericyte recruitment over time. more...
- Published
- 2023
23. Biochemical and Cellular Characterization of PROKR1 and PROKR2 Receptors and Structural Screening of their Ligand PROK1 : Interest for PROK1-Dependent Pathologies
- Author
-
Gemy, Kévin, BioSanté (UMR BioSanté), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Université Grenoble Alpes [2020-....], Mohamed Benharouga, and Nadia Alfaidy-Benharouga more...
- Subjects
Prokr ,Inflammation ,Prokinéticine ,Prok1 ,Eg-Vegf ,Angiogenesis ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,Prokineticin ,Angiogénèse - Abstract
Prokineticin-1 (PROK1) protein, also known as EG-VEGF (Endocrine gland derived-vascular endothelial growth factor) belongs to the prokineticin (PROK) family, whose members are directly involved in the control of key processes such as inflammation and angiogenesis. PROK1 acts via two GPCR receptors, PROKR1 and PROKR2 and regulates several physiological and pathological processes, including cancer and cystic fibrosis (CF). My research team recently demonstrated that the expression of PROK1 and PROKR2 were increased in the female reproductive cancer, gestational choriocarcinoma (GC) and in CF. The antagonization of PROKR2 in an animal model of GC decreased tumor growth and its progression, suggesting that inhibition of the PROK1/PROKR2 system may constitute a promising therapeutic target for all PROK1-dependent cancers and pro-inflammatory diseases. However, anti-PROKR2 antagonists are not specific for this receptor, exhibit long-term toxicity and may be associated with the development of side effects as they block a shared receptor with other members of the PROKs family.The objectives of my thesis project aimed at i) developing a new strategy that targets the inhibition of the ligand, PROK1, and ii) characterizing the molecular and cellular features that differentiate PROKR1 from PROKR2 in order to propose new therapeutic strategies that will specifically target one or the other receptor.The development of the PROK1 blocking strategy required the cloning of the human Prok1 sequence in different plasmids, the implementation of several eukaryotic or prokaryotic expression and production systems, and the development of numerous purification techniques in order to resolve its three-dimensional structure (STD). In silico analyses were also performed to accelerate the STD resolution of PROK1, to characterize the sites of its interaction with PROKR2, and to perform molecular docking in order to identify chemical molecules able to inhibit the PROK1-PROKR2 interaction. A functional assay was also developed to test in vitro the most promising molecules. The functional test required the development of two stable cell lines expressing PROKR1 or PROKR2.The cellular and molecular characterization of PROKR1 and PROKR2 revealed post-translational differences regarding the receptor’s half-life, its stability, trafficking and folding levels. Based on these observations, we initiate the investigation of the mechanisms behind these differences, firstly by targeting the roles of N- and C-terminus in these processes, particularly in the dimerizations of PROKR1and PROKR2.Altogether my thesis project allowed the production of a recombinant PROK1 protein, the resolution of its structure and the identification of the amino acids that are involved in its interaction with PROKR2. The development of cell lines expressing PROKR1 or PROKR2 allowed, i) the set-up of a functional test of the activity of prokineticins, ii) the identification of three inhibiting molecules of the PROK1 ligand and iii) the demonstration that, in spite the existence of a huge identity between PROKR1 and PROKR2, PROKR1 would be more stable at the plasma membrane; undergo less degradation at the lysosome level and its C-terminal part may confer the dimeristaion between PROKRs receptors.In conclusion, my thesis project established new molecular tools for the study of the prokineticins system; identified new inhibiting molecules of the PROK1-PROKR2 interaction, and compared for the first time PROKRs cellular and biochemical features. All these data will allow better targeting of PROKRs and their ligands in order to propose more specific pharmacotherapies for PROK-dependent cancers and inflammatory diseases, such as preeclampsia and cystic fibrosis.; Le facteur, PROK1 aussi dénommé EG-VEGF appartient à la famille des prokinéticines (PROK), dont les membres sont directement impliqués dans le contrôle de l’inflammation et l’angiogenèse. Ces facteurs agissent via deux récepteurs de la famille des RCPG, PROKR1 et PROKR2 et régulent plusieurs processus physiologiques et pathologiques, y compris le cancer. Mon équipe de recherche a récemment démontré que l’expression de PROK1 et PROKR2 était augmentée dans le cancer du système reproducteur féminin, le choriocarcinome gestationnel (CG), et dans la mucoviscidose. L’antagonisation de PROKR2 dans un modèle animal de CG diminue le développement et la progression de ce cancer et réduit la réponse inflammatoire, suggérant que l'inhibition du système PROK1/PROKR2 pourrait constituer une cible thérapeutique prometteuse pour les cancers et les pathologies pro-inflammatoires PROK1-dépendantes. Cependant, les antagonistes anti-PROKR2 ne sont pas spécifiques pour ce récepteur, présentent une toxicité à long terme et peuvent être associés au développement d’effets secondaires. Ainsi, les objectifs de ma thèse étaient, i) de développer une nouvelle stratégie d’inhibition en ciblant le ligand, PROK1, et ii) de mettre en évidence les différences moléculaires et cellulaires des récepteurs PROKR1 et PROKR2 afin de proposer, à long terme, des stratégies thérapeutiques ciblant spécifiquement l’un ou l’autre récepteur. Le développement de la stratégie du blocage du ligand PROK1 a nécessité, le clonage de la séquence humaine de prok1 dans différents plasmides, la mise en place de plusieurs systèmes « eucaryotes ou procaryotes » d’expression et de production, et le développement de nombreuses techniques de purification afin de résoudre sa structure tridimensionnelle (STD). Des analyses in silico ont également été réalisées ; pour accélérer la résolution de sa STD, caractériser les interfaces de son interaction avec PROKR2, et réaliser le docking moléculaire dans le but d’identifier des molécules chimiques capables d’inhiber l’interaction PROK1-PROKR2. Un test fonctionnel a aussi été développé pour évaluer in vitro les molécules les plus prometteuses. Pour la réalisation de ce test fonctionnel, nous avons généré deux lignées stables exprimant PROKR1 ou PROKR2. La caractérisation cellulaire et moléculaire de PROKR1 et PROKR2 a mis en évidence l’existence de différences post-traductionnelles concernant les demi-vies des récepteurs, leur stabilité, leur mode d’adressage à la membrane et leurs niveaux de repliement. Partant de ce constat, nous avons débuté l’étude des mécanismes sous-jacents à ces différences en ciblant prioritairement les extrémités N- et C-ter et leurs implications dans le processus de dimérisation potentielle de PROKR1 et PROKR2.L’ensemble des résultats de ma thèse ont permis de produire la protéine PROK1, de résoudre sa structure et d’identifier les acides aminés impliqués dans son interaction avec le PROKR2. Le développement des lignées exprimant PROKR1 ou PROKR2 a permis, i) de mettre en place un test fonctionnel de l’activité des prokinéticines, ii) d’identifier trois molécules inhibitrices du ligand PROK1 et iii) de démontrer qu’en dépit de la faible différence d’identité entre PROKR1 et PROKR2, que PROKR1serait plus stable au niveau de la membrane, subirait moins de dégradation au niveau du lysosome, et que sa partie C-ter confèrerait la dimérisation entre récepteurs PROKRs.En conclusion, ma thèse a apporté des outils moléculaires pour l’étude des prokinéticines, a permis d’identifier de nouvelles molécules inhibitrices du ligand PROK1 et de son interaction avec PROKR2 et à comparer pour la première fois les deux récepteurs de cette famille. L’ensemble de ces données nous permettra de mieux cibler les récepteurs PROKRs et leurs ligands afin de proposer une pharmacothérapie plus spécifique des cancers PROK-dépendants et des maladies inflammatoires dans lesquelles ils sont dérégulés, comme la prééclampsie et la mucoviscidose. more...
- Published
- 2023
24. Investigating the efficacy of chemotherapeutics and the effect of an antiangiogenic drug in tumor models
- Author
-
Say, Patrick
- Subjects
Krebstherapie ,Cancer therapy ,Tumorwachstum ,Chemotherapy ,Angiogenesis ,Chemotherapie ,Angiogenese ,VEGF ,Tumor growth - Abstract
Das Ziel dieser Bachelorarbeit ist es, die Bedeutung der Angiogenese bei der Tumorentstehung und deren therapeutischen Ansatz zu erläutern. Außerdem soll die Wirksamkeit der Chemotherapeutika 5-FU und Cisplatin sowie die der Behandlung von Blutgefäßen mit dem monoklonalen Anti-VEGF-Antikörper BD0801 untersucht werden. Allgemein, ist Krebs eine der tödlichsten Krankheiten der Welt, da Tumore in fast allen Organen und Geweben des menschlichen Körpers entstehen können, was das breite Spektrum von Tumorarten erklärt. Für die Entstehung eines bösartigen Tumors sind sechs Krebsmerkmale entscheidend. Ein wichtiges Merkmal ist die Angiogenese, welche Krebszellen, über die Bildung von Blutgefäßen, mit Sauerstoff und Nährstoffen versorgt. Dieser Prozess führt zu abnormalem Tumorwachstum und Metastasenbildung. Die Bekämpfung der Zellproliferation und der Aufrechterhaltung der Angiogenese in Tumorzellen ist ein vielversprechendes Konzept, das vielen Krebspatienten das Leben retten könnte. Daher wurde die Wirksamkeit der Chemotherapeutika 5-FU und Cisplatin in einem Brustdrüsenkrebsmausmodell (4T1) und einem duktalen Adenokarzinom der Bauchspeicheldrüse der Maus (K518WT) untersucht. Darüber hinaus wurden die Blutgefäße in den Tumorzellen gefärbt und mit verschiedenen Organen verglichen, um die strukturellen Unterschiede aufzuklären. Außerdem wurde der derzeit untersuchte monoklonale Anti-VEGF-Antikörper BD0801 im K518WT-Tumormodell getestet. Die Gewebefärbung hat gezeigt, dass die Tumorgefäße im Vergleich zu gesunden Gefäßen weitgehend desorganisiert sind. Die chemotherapeutischen Tests haben eine signifikante antiproliferative Wirkung auf beide Tumorarten gezeigt. BD0801 hat vielversprechende Auswirkungen auf die behandelten Blutgefäße gezeigt, da das Gefäßsystem zurückgebildet war. Zusammengefasst waren die durchgeführten Versuche erfolgreich und zeigen damit ein großes Potenzial als Kombinationstherapie mit Chemotherapeutika und BD0801. The aim of this bachelor thesis is to elucidate the importance of angiogenesis in tumor formation and its therapeutic approach. Additionally, the goal was to evaluate the efficacy of the chemotherapeutics 5-FU and Cisplatin, as well as to investigate the effectiveness of treating blood vessels using the anti-VEGF monoclonal antibody BD0801. Generally, cancer is one of the most lethal diseases on the planet since solid tumors originate from almost every organ or tissue in the human body, resulting in a wide range of tumor types. In the formation and maintenance of a malignant tumor, so far, six cancer hallmarks have been identified. A significant hallmark is angiogenesis, which provides oxygen and nutrients for cancer cells via blood vessel formation, thus leading to tumor growth and metastasis. Tackling the cell proliferation and angiogenesis in tumor cells is a promising therapeutic concept that could save many cancer patients’ lives. In this bachelor thesis, the efficacy of the chemotherapeutics 5-FU and Cisplatin in a murine mammary gland carcinoma (4T1) and a mouse pancreatic ductal adenocarcinoma (K518WT) tumor model was evaluated. Furthermore, blood vessels in tumor tissue were stained and the results were compared with different organs to elucidate the structural differences. Moreover, the currently investigated anti-VEGF monoclonal antibody BD0801 was tested in the K518WT tumor model. The tumor tissue staining demonstrated that tumor vessels are extensively disorganized compared to healthy vessels. The chemotherapeutic tests revealed a significant antiproliferative effect on both tumor cell lines. Furthermore, BD0801 showed promising effects on inhibiting the growth of blood vessels. After the treatment, the vasculature was recessed and disconnected. In conclusion, the conducted experiments were successful, stressing the potential of a combination therapy consisting of chemotherapeutics and BD0801. more...
- Published
- 2023
25. Einfluss Endothelialer Progenitorzellen auf die Revaskularisation mikrovaskulärer anastomosierter Fernlappentransplantate
- Author
-
Jäger, Lukas and Justus Liebig University Giessen
- Subjects
Lappentransplantate ,Stammzellen ,ddc:610 ,Ratten ,Tierversuch ,ddc:630 ,EPC ,Mikrovaskuläre Anastomosen ,Angiogenese ,Endotheliale Progenitorzellen - Abstract
Freie Lappentransplantate sind eine verbreitete, aber sehr anspruchsvolle Methode der plastischen Chirurgie zur Deckung komplizierter Wunden. Um Komplikationen bei diesen Eingriffen zu verringern, könnte der Einsatz von Endothelialen Progenitorzellen (EPC) nützlich sein. Diese Zellen haben in vielen Untersuchungen positive Effekte auf die Neovaskularisation von Geweben gezeigt. Teils bewirken sie über parakrine Effekte eine Steigerung der Angiogenese oder werden direkt in neue Gefäße inkorporiert. In der vorliegenden Studie wird daher die Hypothese untersucht, dass EPC die Vaskularisation von freien Lappentransplantaten und damit deren Wundheilung verbessern. Im Tiermodell werden freie Lappen transplantiert, wobei zunächst die von Miyamoto und Kollegen beschriebene Operationsmethode in einigen Punkten modifiziert werden muss, um die Erfolgsrate zu steigern. Ebenfalls werden im Rahmen eines Vorversuchs die Gefäßarchitektur und histologische Parameter zu verschiedenen Entnahmezeitpunkten verglichen. Der siebte postoperative Tag wird als Entnahmezeitpunkt für den Hauptversuch ausgewählt, da sich hier die größten Unterschiede zwischen den Transplantaten und den Kontrollgeweben zeigen. Im Hauptversuch erhalten die Tiere entweder subkutan oder intraarteriell EPC bzw. in der Kontrollgruppe keine EPC. An den entnommenen Transplantaten wird histologisch die Vaskularisation und elektronenmikroskopisch (Raster-Elektronen-Mikroskop, REM) die Gefäßarchitektur untersucht. Auf eine Zugdehnungsmessung der Wundränder muss aufgrund der hohen Fragilität der Wundränder verzichtet werden. Histologisch kann eine signifikante Steigerung der Gefäßanzahl bei den Behandlungsgruppen um 39 % (EPC intraarteriell) bzw. 45 % (EPC subkutan) gegenüber der Kontrollgruppe festgestellt werden, wobei keine Applikationsart der EPC der anderen überlegen ist. Hinsichtlich der Dicke der Hautschichten und der Proliferationsrate ergaben sich keine signifikanten Unterschiede. Bei der Untersuchung der dreidimensionalen Gefäßarchitektur (Gefäßdurchmesser, Gefäßabstand, Aufzweigungswinkel und Abstand der Gefäßaufzweigungen) ergeben sich keine signifikanten Unterschiede, welche gegebenenfalls zu einem früheren Untersuchungszeitpunkt darstellbar sein könnten. In den REM-Untersuchungen lässt sich kein signifikanter Unterschied im Auftreten von pillars und sprouts feststellen, da vermutlich der Großteil der SA bereits vor dem Untersuchungszeitpunkt passiert und die IA erst anzulaufen beginnt. Die aus anderen Anwendungsgebieten bekannten positiven Effekte der EPC auf die Neovaskularisation können anhand dieser Studie auch bei freien Lappentransplantaten nachgewiesen werden. Aufgabe zukünftiger Studien wäre es, die Art und Weise der proangiogenen Wirkungen zu untersuchen und den Einfluss der EPC auf die funktionelle Wundheilung, z.B. durch Überprüfung der Wundfestigkeit. Veterinärmedizinisch bedeutsam wäre die weitere Erforschung von spezieseigenen EPC und deren Effekten im Tierkörper. more...
- Published
- 2023
- Full Text
- View/download PDF
26. Characterization of CEACAM1 in retina and choroid in mouse model
- Author
-
Müller, Jonathan
- Subjects
Netzhaut ,ddc:610 ,610 Medizin und Gesundheit ,Aderhaut ,Angiogenese ,Vaskularisation - Abstract
Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) ist ein multifunktionales Zell-Zell Adhäsionsprotein, das in eine Vielzahl an zellulären Prozessen involviert ist, wie zum Beispiel der Differenzierung von Geweben, der Tumorsuppression, Metastasierung, Angiogenese und Apoptose. Außerdem hat es modulierende Eigenschaften auf die angeborene und erworbene Immunantwort. In der vorliegenden Arbeit charakterisierte ich initial die Lokalisation und die CEACAM1-exprimierenden Zelltypen im Auge und bestimmte quantitativ die Expression von Ceacam1 in der Retina und Choroidea zu unterschiedlichen Zeitpunkten. Es zeigte sich hierbei, dass Ceacam1 zu allen untersuchten Zeitpunkten, sowohl während der Entwicklung als auch im adulten retinalen und choroidalen Gewebe nachweisbar war. Mittels Immunhistochemie konnte die Expression von CEACAM1 im Corneaepithel, den Gefäßen der Iris und des Ziliarkörpers, im nicht-pigmentierten Epithel des Ziliarkörpers, sowie in den retinalen und choroidalen Gefäßen nachgewiesen werden. Durch Doppelfärbung mit Kollagen IV konnte die endotheliale Expression von CEACAM1 in den Endothelzellen der Gefäße bestätigt werden. Im zweiten Teil meiner Arbeit untersuchte ich die Funktion von CEACAM1 im Auge und verglich dazu wildtypische Retinae mit Cc1-/--Retinae. Es zeigten sich keine offensichtlichen morphologischen Veränderungen der retinalen Schichten und die anschließend durchgeführten morphometrischen Analysen der Schichtdicken der retinalen Neurone zeigte keine Anzeichen einer Neurodegeneration. Allerdings waren in Cc1-/--Retinae kleine Zysten und IBA1 positive, phagozytisch aktive Zellen im subneuroretinalen Raum, also dem Bereich zwischen RPE und den Außensegmenten der Photorezeptoren zu erkennen. Die anschließend durchgeführten Expressionsanalysen immunmodulierender Faktoren und von Mitgliedern des TGF-β-Signalwegs in retinalen und choroidealen Proben wildtypischer und Cc1-/--Mäusen zeigten keine veränderte Expression für Iba1, Ccl2 sowie Tnf-α. Jedoch konnten signifikant erhöhte Werte für TGF-β1 in der Gruppe der 2-4 als auch der Gruppe der 9 Monate alten Cc1-/--Retinae im Vergleich zu wildtypischen Retinae nachgewiesen werden. Basierend auf den Daten der vorliegenden Arbeit kann geschlussfolgert werden, dass die Deletion von CEACAM1 unter physiologischen Bedingungen die Struktur der Retina und Choroidea nicht offensichtlich beeinflusst. Allerdings führt die Deletion zu erhöhten Tgfβ1 Spiegeln in der Retina und zur Aktivierung und Akkumulation von IBA1 positiven Zellen im subneuroretinalen Raum., Carcinoembryonic antigen-related cell adhesion molecule 1 (Ceacam1, Cc1) is a multi-functional cell– cell adhesion protein, involved in the differentiation and arrangement of tissue three-dimensional structure, tumor suppression, metastasis, angiogenesis and apoptosis. Moreover, it has been shown to modulate innate and adaptive immune responses. Amongst others, it is expressed in vascular endothelial cells during vascular development and in adult blood vessels that are activated by angiogenic processes. In this study, we aimed to learn about the function of Ceacam1 in the eye. We therefore characterized the cell type specific expression of Ceacam1 in the ocular tissues, analyzed its retinal and choroidal expression at different developmental time points and studied the ocular morphology of Ceacam1- knockout (Cc1-/-) mice. Finally, we analyzed the molecular expression levels of immune modulating factors and members of the TGF-β signaling family in retinal and choroidal tissues of Cc1-/- mice. Our data show that Ceacam1 is expressed in developing and mature retinal and choroidal endothelial cells. Furthermore, its deletion promotes the accumulation of phagocytic active and Iba1-positive cells in the subretinal space and significant upregulated TGF-β1 expression levels in the retina. more...
- Published
- 2023
- Full Text
- View/download PDF
27. A linfangiogênese em enxertos corneanos humanos que evoluem para retransplante
- Author
-
Karine Feitosa Ximenes, Karla Feitosa Ximenes Vasconcelos, and Fernando Queiroz Monte
- Subjects
Linfangiogênese ,Angiogênese ,Transplante de córnea ,Ceratoplastia ,Reoperação ,Ophthalmology ,RE1-994 - Abstract
RESUMO Objetivo: Estudar botões corneanos humanos com linfangiogênese através do exame histopatológico, juntamente com os enxertos de seus transplantes anteriores e posteriores, avaliando os intervalos de tempo para sucessivas cirurgias. Métodos: Estudo descritivo, observacional, longitudinal de botões corneanos humanos com linfangiogênese, juntamente com seus transplantes anteriores e posteriores. Os tecidos foram provenientes de ceratoplastia penetrante no período compreendido entre os anos 2006 e 2013. Após revisão de prontuários em que foram obtidas principalmente as datas das cirurgias, construímos uma tábua de sobrevivência a partir da qual os intervalos de tempo para retransplante foram calculados. Resultados: Entre 89 casos de linfangiogênese corneana, foram incluídos apenas aqueles 22 que possuíam registros no prontuário de transplantes anteriores ou posteriores. Nos casos que apresentavam como provável etiologia do retransplante a linfangiogênese, isolada ou associada à hemangiogênese (grupos pré-linfangiogênese/linfangiogênese e interlinfangiogênese), foram encontrados intervalos de tempo para retransplante menores (7 e 3 meses, respectivamente) que aquele encontrado no grupo linfangiogênese/pós-linfangiogênese que apresentava outras etiologias prováveis para os retransplantes (11,31 meses). Casos que apresentavam como etiologia provável do retransplante a linfangiogênese isolada apresentaram um intervalo para retransplante (3 meses) ainda menor que aquele encontrado nos casos em que a etiologia provável era a linfangiogênese associada à hemangiogênese (7,80 meses). Conclusão: Linfangiogênese, isolada ou associada à hemangiogênese, foi encontrada nos enxertos corneanos humanos estudados que evoluíram para retransplante em pequenos intervalos de tempo. Esse achado nos leva a sugerir um possível papel para os vasos linfáticos na redução do tempo de sobrevida dos enxertos corneanos humanos. more...
- Published
- 2015
- Full Text
- View/download PDF
28. Les thérapies ciblées per os.
- Author
-
Étienne-Selloum, Nelly
- Abstract
Copyright of Actualités Pharmaceutiques is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
- Published
- 2018
- Full Text
- View/download PDF
29. Surgical sponge associated with platelets rich plasma in skin mesh grafts and layer in rabbits (Oryctolagus cuniculus).
- Author
-
Pazzini, J. M., Serafim, E. L., Uscategui, R. R. A., Almeida, V. T., Oliva, C. A. C., Gärtner, F., Amorim, I., Faria, F., Rêma, A., Filho, N. P. Reis, Ferreira, M. G. P. A., Silva, A. C., Huppes, R. R., Moraes, P. C., Oliveira, J. A., and Nardi, A. B. more...
- Abstract
Copyright of Arquivo Brasileiro de Medicina Veterinária e Zootecnia is the property of Universidade Federal de Minas Gerais, Escola de Veterinaria and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
- Published
- 2018
- Full Text
- View/download PDF
30. Acute endurance exercise increases Vegfa mRNA expression in adipose tissue of rats during the early stages of weight gain.
- Author
-
Ludzki, Alison C., Pataky, Mark W., Cartee, Gregory D., and Horowitz, Jeffrey F.
- Subjects
- *
ADIPOSE tissues , *ANIMAL experimentation , *CELLULAR signal transduction , *EXERCISE physiology , *GENE expression , *NEOVASCULARIZATION , *PHYSICAL fitness , *RATS , *WEIGHT gain , *VASCULAR endothelial growth factors - Abstract
The aim of this study was to determine the effects of acute exercise on key factors regulating angiogenesis in adipose tissue. Adipose tissue Vegf-a messenger RNA expression was upregulated immediately after acute exercise ( p < 0.05) in rats consuming a high-fat diet, but was lower after exercise ( p < 0.05) in rats consuming a low-fat diet. Our working hypothesis is that acute exercise augments angiogenic signaling under conditions when adipose tissue is expanding, and with repeated exercise sessions these signals can accrue to enhance vascularization. [ABSTRACT FROM AUTHOR] more...
- Published
- 2018
- Full Text
- View/download PDF
31. Pathophysiology of chronic limb ischemia.
- Author
-
Simon, F., Oberhuber, A., Floros, N., Düppers, P., Schelzig, H., and Duran, M.
- Abstract
Chronic ischemia of the lower extremities is an everyday problem in vascular surgery clinics. In Germany, approximately 3% of all hospitalizations are due to peripheral artery disease (PAD), with critical limb ischemia (CLI) in particular showing a rapid increase. The consequences of chronic undersupply range from reduced walking distance to loss of limbs. At the beginning there are stress factors, such as hyperlipidemia (LDL), free radicals, arterial hypertension, infections or subclinical inflammation that interfere with endothelial homeostasis and cause endothelial dysfunction with increased permeability. Cells of the immune system are attracted and migrate into the vascular wall, where they lead to the degradation of matrix components and destabilization of the plaque. By changing the phenotype of smooth muscle cells and macrophages towards osteoclast-like cells, bone-like hardening of the vessel wall takes place. Above a vessel wall thickness of approximately 100 µm, hypoxia-induced factor (HIF-1α) is intensified by the lack of oxygen, which leads to an increase in growth factors, such as vascular endothelial growth factor (VEGF). This promotes angiogenesis, but it is not sufficient to compensate for a stenosed artery. Arteriogenesis refers to the growth of existing collateral vessels. The driving forces are the pressure gradient before and after the stenosis and the shear forces acting on the vessel walls. In the case of progressive stenosis, the compensatory capacities can be overtaxed and a manifest hypoxia in the tissue with regression of the obtained vascular structures and tissue atrophy occurs. [ABSTRACT FROM AUTHOR] more...
- Published
- 2018
- Full Text
- View/download PDF
32. Membrana de látex natural de Hevea brasiliensis auxilia no processo de reparação tecidual em bovinos.
- Author
-
Zimmermann, M., Mendes, F. F., Rodrigues, D. F., Faleiro, M. R., Campos, G. S., and Araújo, E. G.
- Abstract
Copyright of Arquivo Brasileiro de Medicina Veterinária e Zootecnia is the property of Universidade Federal de Minas Gerais, Escola de Veterinaria and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
- Published
- 2018
- Full Text
- View/download PDF
33. Principais achados do exame histopatológico de botões corneanos humanos com linfangiogênese
- Author
-
Karine Feitosa Ximenes, Jailton Vieira Silva, Karla Feitosa Ximenes Vasconcelos, and Fernando Queiroz Monte
- Subjects
Linfangiogênese ,Angiogênese ,Córnea/patologia ,Ceratoplastia ,Ophthalmology ,RE1-994 - Abstract
Objetivo: Detectar a presença de vasos linfáticos na córnea através do exame histopatológico, buscando identificar os achados que são encontrados com maior frequência junto à presença desses vasos nesse tecido. Métodos: Estudo retrospectivo descritivo de botões corneanos humanos com linfangiogênese. Os tecidos foram provenientes de ceratoplastia penetrante no período compreendido entre os anos de 2006 e 2013. Foi realizada revisão de prontuários em busca de informações sobre sexo, idade e etiologia do transplante. Resultados: Foram incluídos 89 botões corneanos, sendo 37 de pacientes do sexo feminino e 52 do sexo masculino. A média das idades foi de 47,70 ± 23,95 anos (média ± DP). Linfangiogênese foi encontrada principalmente associada à hemangiogênese. Linfangiogênese isolada, no entanto, foi observada em 28 (31,46%) casos. Em 18 (20,22%) casos foram encontrados uma quantidade de vasos linfáticos cerca de quatro vezes maior que aquela encontrada na maioria das amostras. Em um grande número de casos foram encontrados condições inflamatórias como infecção e perfuração. Nas proximidades da linfangiogênese, encontramos muitos casos de sinéquia anterior e miofibroblastos. Em 17 (16,35%) casos nenhuma alteração foi evidenciada nas proximidades dos vasos linfáticos corneanos. Conclusão: Demonstramos, através do exame histopatológico, que achados reconhecidamente associados à linfangiogênese, como os processos inflamatórios, são encontrados também com frequência em casos de córneas humanas que possuem vasos linfáticos. Porém, outros achados evidenciados, como a linfangiogênese desacompanhada de angiogênese, a presença de uma maior quantidade de vasos em alguns casos e a linfangiogênese sem alterações em sua proximidade, permanecem necessitando de uma melhor compreensão. more...
- Published
- 2015
- Full Text
- View/download PDF
34. Radiotherapy and esophageal cancer; towards novel and improved combination treatments
- Subjects
Combinatie behandeling ,Radiotherapy ,Combination treatments ,Vaccin ,Esophageal cancer ,Respons ,Treatment response ,Angiogenese ,Tumor microenvironment ,SDG 3 - Good Health and Well-being ,Tumor micromilieu ,Interferon ,Immunotherapy ,Angiogenesis ,Radiotherapie ,Slokdarmkanker ,Vaccine ,Immunotherapie - Abstract
Esophageal cancer is a disease characterized by a substantial genomic- and immunological heterogeneity and dismal treatment outcomes. In this thesis, several molecular and immunological factors influencing the treatment outcome of patients with localized esophageal cancer were identified, with a key role for T cell mediated immunity and a type I interferon response. Treatment outcomes of localized esophageal cancer might be further improved by comprehensive upfront tumor microenvironment analysis and selection of patients with immunosuppressive traits for immunomodulation before chemoradiotherapy. more...
- Published
- 2022
35. Radiotherapy and esophageal cancer; towards novel and improved combination treatments
- Author
-
Goedegebuure, Ruben Sije Adriaan, Verheul, Hendrik Marinus Willem, de Gruijl, Tanja, Derks, S., Thijssen, V.L.J.L., VUmc - School of Medical Sciences, Derks, Sarah, Thijssen, Victor, VU University medical center, and Internal medicine more...
- Subjects
Combinatie behandeling ,Radiotherapy ,Combination treatments ,Vaccin ,Esophageal cancer ,Respons ,Treatment response ,Angiogenese ,Tumor microenvironment ,SDG 3 - Good Health and Well-being ,Tumor micromilieu ,Interferon ,Immunotherapy ,Angiogenesis ,Radiotherapie ,Slokdarmkanker ,Vaccine ,Immunotherapie - Abstract
Esophageal cancer is a disease characterized by a substantial genomic- and immunological heterogeneity and dismal treatment outcomes. In this thesis, several molecular and immunological factors influencing the treatment outcome of patients with localized esophageal cancer were identified, with a key role for T cell mediated immunity and a type I interferon response. Treatment outcomes of localized esophageal cancer might be further improved by comprehensive upfront tumor microenvironment analysis and selection of patients with immunosuppressive traits for immunomodulation before chemoradiotherapy. more...
- Published
- 2022
36. In-vitro Vergleich der osteogenen und angiogenen Eigenschaften der bioaktiven Gläser 45S5, 1393 und 0106B1
- Author
-
Kunisch, Elke, Saur, Merve, Fiehn, Linn Anna, Arango-Ospina, Marcela, Merle, Christian, Boccaccini, Aldo R., Renkawitz, Tobias, and Westhauser, Fabian
- Subjects
Osteogenese ,Biomaterialien ,bioaktives Glas ,Medicine and health ,mesenchymale Stromazellen ,Angiogenese - Abstract
Fragestellung: Das initial von Hench entwickelte 45S5-bioaktive Glas (BG) weist trotz seiner herausragenden bioaktiven Eigenschaften einige Limitationen auf. Daher wird nach alternativen Glaskompositionen für die Gewährleistung einer optimalen Regeneration von knöchernen Defekten gesucht. [zum vollständigen Text gelangen Sie über die oben angegebene URL] more...
- Published
- 2022
- Full Text
- View/download PDF
37. Die Proteinkinase CK2 : Ein neuer Regulator des Oberflächenproteins nerve/glial antigen (NG)2
- Author
-
Schmitt, Beate Maria
- Subjects
Glioblastoma multiforme (GBM) ,Nerve/glial antigen 2 (NG2) ,Perizyt ,Proteinkinase CK2 ,Glioblastoma ,Angiogenese - Published
- 2022
- Full Text
- View/download PDF
38. Évaluation des propriétés angiogéniques des biomatériaux de coiffage pulpaire
- Author
-
Tessonnier, Margot, Aix-Marseille Université - Faculté des sciences médicales et paramédicales (AMU SMPM), Aix Marseille Université (AMU), and Thomas Giraud
- Subjects
Coiffage pulpaire direct ,Cellules endothéliales ,MESH: Coiffage pulpaire ,MESH: Agents de coiffage pulpaire et de pulpectomie ,[SDV.MHEP.CHI]Life Sciences [q-bio]/Human health and pathology/Surgery ,Fibroblastes pulpaires ,MESH: Cellules endothéliales ,MESH: Facteurs de croissance fibroblastique ,Angiogenèse ,MESH: Néovascularisation physiologique ,Biomatériaux ,MESH: Pulpe dentaire-cytologie ,MESH: Fibroblastes ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology - Abstract
Maintaining pulp vitality is a major issue in dentistry. Inflammation control and regeneration are essential prerequisites. Within these events, angiogenesis is defined as the development of capillary vessels from pre-existing capillaries. Consecutively to pulp exposure, numerous direct pulp capping materials are available to allow dental organ vitality preservation whether following the progression of a carious lesion or traumatic lesion. The aim of this study was to evaluate the consequences of the application of selected capping biomaterials (BiodentineTM, TheraCal LC®, and a universal adhesive, All Bon Universal®) on key steps of angiogenesis. Thus, this work focused on two distinct cell populations, pulp fibroblasts and endothelial cells by simulating a carious lesion using a bacterial pattern and performing physical cell damage. Growth factor secretion (PDGF, FGF-2 and VEGF), cell migration and proliferation were studied. The important notions related to angiogenesis in a context of direct pulp capping will be recalled in the first part of the work. Subsequently, all the methods used for this experimental work will be presented as well as the results obtained. These will be finally discussed in the last part of the manuscript.; Le maintien de la vitalité pulpaire est un enjeu majeur en odontologie. Le contrôle de l’inflammation et la mise en place de la régénération en sont des prérequis indispensables. Au sein de ces évènements l’angiogenèse se définit comme le développement de vaisseaux capillaires à partir de capillaires préexistants. Lors d’une effraction pulpaire, différents matériaux de coiffage direct sont disponibles pour permettre la conservation de la vitalité de l’organe dentaire que ce soit consécutivement à la progression d’une lésion carieuse ou suite à une lésion traumatique. Le but de cette étude a été d’évaluer les conséquences de l’application des biomatériaux de coiffage choisis (BiodentineTM, TheraCal LC®, et un adhésif universel le All Bon Universal®) sur des étapes clés de l’angiogénèse. Ainsi, ce travail a porté sur deux populations cellulaires distinctes, les fibroblastes pulpaires et les cellules endothéliales en simulant une lésion carieuse à l’aide d’un motif bactérien et de la réalisation de lésions cellulaires physiques. La sécrétion de facteur de croissance (PDGF, FGF-2 et VEGF), la migration et la prolifération cellulaire ont été étudiées. Les notions importantes liées à l’angiogenèse dans un contexte de coiffage pulpaire seront rappelées dans une première partie du travail. Par la suite, l’ensemble des méthodes utilisées pour ce travail expérimental seront présentées ainsi que les résultats obtenus. Ces derniers seront enfin discutés dans la dernière partie du manuscrit. more...
- Published
- 2022
39. Élaboration d'un protocole de recherche impliquant la personne humaine : intérêt de la TEP au [ 68 Ga]Ga-RGD dans la maladie du Rendu-Osler
- Author
-
Daverton, Florie, Aix-Marseille Université - Faculté de pharmacie (AMU PHARM), Aix Marseille Université (AMU), and Philippe Garrigue
- Subjects
Recherche impliquant la personne humaine ,Angiogenèse ,[SDV]Life Sciences [q-bio] ,Maladie de Rendu-Osler ,Médicament radiopharmaceutique ,[SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences - Abstract
La maladie de Rendu-Osler (MRO) est une maladie rare, héréditaire, due à une dysfonction de l’angiogenèse. Les principales manifestations cliniques consistent en l’apparition de télangiectasies et de malformations artério-veineuses (MAV). Le bevacizumab, anticorps monoclonal anti-VEGF à visée anti-angiogénique, est décrit comme ayant un intérêt dans la prise en charge de certaines MAV de la MRO, principalement au niveau hépatobiliaire et digestif. Cependant, aucun biomarqueur robuste n’existe pour orienter son emploi dans la MRO. Nous avons posé l’hypothèse que disposer d’une imagerie moléculaire isotopique de l’activité angiogénique permettrait de mieux orienter la stratégie thérapeutique et s’assurer de la réponse ou d’un échappement au traitement anti-angiogénique. Le [68Ga]Ga-NODAGA-RGD, médicament radiopharmaceutique expérimental pour la tomographie par émission de positons (TEP), cible avec une haute affinité les intégrines αvß3 jouant un rôle déterminant dans l’activation angiogénique et dont la surexpression a été mis en évidence dans les MAV. L’objectif de cette thèse est de démontrer l’intérêt que pourrait avoir ce radiotraceur dans la prise en charge de la MRO et l’élaboration d’un protocole de recherche impliquant la personne humaine associant centre de référence pour la MRO, biologie médicale, médecine nucléaire et radio pharmacie. more...
- Published
- 2022
40. Pathophysiologie der chronisch-kritischen Extremitätenischämie.
- Author
-
Simon, F., Oberhuber, A., Floros, N., Düppers, P., Schelzig, H., and Duran, M.
- Abstract
Copyright of Gefaesschirurgie is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
- Published
- 2018
- Full Text
- View/download PDF
41. Histochemical and immunohistochemical evaluation of angiogenesis in rabbits (Oryctolagus cuniculus) submitted to skin grafts associated with platelet-rich plasma.
- Author
-
Pazzini, Josiane M., Serafim, Eduardo L., Gärtner, Fátima, Amorim, Irina, Faria, Fátima, Rêma, Alexandra, Moraes, Paola C., and De Nardi, Andrigo B.
- Abstract
Copyright of Pesquisa Veterinaria Brasileira is the property of Colegio Brasileiro de Patologia Animal - CBPA and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
- Published
- 2017
- Full Text
- View/download PDF
42. Tumor-associated Macrophages, Angiogenesis, and Tumor Cell Migration in Oral Squamous Cell Carcinoma.
- Author
-
Udeabor, Samuel E., Adisa, Akinyele O., Orlowska, Anna, Sader, Robert A., and Ghanaati, Shahram
- Subjects
- *
SQUAMOUS cell carcinoma , *CARCINOMA , *MACROPHAGES , *TUMORS , *CELL migration , *NEOVASCULARIZATION - Abstract
Objective: To investigate the relationship between tumor-associated macrophages (TAMs), neovascularization, and tumor cell migration in oral squamous cell carcinoma (OSCC) of an African subpopulation. Materials and Methods: Twenty OSCC paraffin blocks underwent immunohistochemistry to TAM1 (CCR7), TAM2 (CD206), Twist, E-cadherin, N-cadherin, and CD34. The relative percentage of CCR7 + and CD206 + cells to overall immune cell population was calculated for three high power fields and an average was taken. TAM-related microvessel density (MVD) was determined as the mean of the three recorded values. Cases that had no CD34 + vessels adjacent to the TAMs region were regarded as having an MVD score of 0. Results: Ten cases (50%) expressed greater CCR7 activity than CD206, seven cases (35%) expressed approximately equal activity of CCR7 and CD206, while three cases (15%) expressed greater activity of CD206 than CCR7. Twist expression was strong in some cases with strong N-cadherin and weak E-cadherin, but the expression of Twist was not consistently high in all cases that expressed strong N-cadherin and weak E-cadherin. Conclusions: TAMs distribution suggested antitumor activity and the potential for tumor metastasis was only partly due to Twist-mediated epithelial-mesenchymal transition. [ABSTRACT FROM AUTHOR] more...
- Published
- 2017
- Full Text
- View/download PDF
43. Thyroid hormones affect decidualization and angiogenesis in the decidua and metrial gland of rats.
- Author
-
Souza, Cíntia A., Silva, Juneo F., Silva, Camila L. R., Ocarino, Natália M., and Serakides, Rogéria
- Abstract
Copyright of Pesquisa Veterinaria Brasileira is the property of Colegio Brasileiro de Patologia Animal - CBPA and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
- Published
- 2017
- Full Text
- View/download PDF
44. Carbon dioxide water-bath treatment augments peripheral blood flow through the development of angiogenesis.
- Author
-
Xu, Yan-Jun, Elimban, Vijayan, and Dhalla, Naranjan S.
- Subjects
- *
CARBON dioxide , *BLOOD flow , *NEOVASCULARIZATION , *HINDLIMB , *VASCULAR endothelial growth factors - Abstract
In this study, we investigated the effects of CO2 water-bath therapy on blood flow and angiogenesis in the ischemic hind limb, as well as some plasma angiogenic factors in peripheral ischemic model. The hind limb ischemia was induced by occluding the femoral artery for 2 weeks in rats and treated with or without CO2 water-bath therapy at 37 °C for 4 weeks (20 min treatment every day for 5 days per week). The peak blood flow and minimal and mean blood flow in the ischemic skeletal muscle were markedly increased by the CO2 water-bath therapy. This increase in blood flow was associated with development of angiogenesis in the muscle, as well as reduction in the ischemia-induced increase in plasma malondialdehyde levels. Although plasma vascular endothelial growth factor and nitric oxide levels were increased in animals with peripheral ischemia, the changes in these biomarkers were not affected by CO2 water-bath therapy. These results suggest that augmentation of blood flow in the ischemic hind limb by CO2 water-bath therapy may be due to the development of angiogenesis and reduction in oxidative stress. [ABSTRACT FROM AUTHOR] more...
- Published
- 2017
- Full Text
- View/download PDF
45. Excesso de tiroxina materna associado ao hipertireoidismo pós-natal reduz o crescimento ósseo e o perfil proliferativo e angiogênico das cartilagens de crescimento de ratos.
- Author
-
Ribeiro, L. G. R., Silva, J. F., Ocarino, N. M., Melo, E. G., and Serakides, R.
- Abstract
Copyright of Arquivo Brasileiro de Medicina Veterinária e Zootecnia is the property of Universidade Federal de Minas Gerais, Escola de Veterinaria and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
- Published
- 2017
- Full Text
- View/download PDF
46. Involvement of mast cells, CD68+ and VEGF+ expressions in response to Himatanthus drasticus commercial latex in mice wound healing model.
- Author
-
Santos, G. J. L., Ferreira, T. C., Rodrigues, A. L. M., Freitas, J. C. C., Morais, S. M., Girão, V. C. C., and Nunes-Pinheiro, D. C. S.
- Abstract
Copyright of Arquivo Brasileiro de Medicina Veterinária e Zootecnia is the property of Universidade Federal de Minas Gerais, Escola de Veterinaria and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
- Published
- 2017
- Full Text
- View/download PDF
47. Bisphenol A interferes with swine vascular endothelial cell functions.
- Author
-
Basini, Giuseppina, Bussolati, Simona, Grolli, Stefano, Ramoni, Roberto, and Grasselli, Francesca
- Subjects
- *
BISPHENOL A , *PHENOLS , *ANIMAL health , *HEALTH , *NEOVASCULARIZATION - Abstract
Several studies have demonstrated that the endocrine disruptor bisphenol A (BPA) negatively affects animal and human health. An angiogenic process has been suggested among the events disrupted by this molecule, but the underlying mechanisms have not yet been clarified. The effect of BPA on angiogenesis was investigated by means of a bioassay previously validated in our laboratory. Using immortalized swine aortic endothelial cell line (AOC), the development of new blood vessels through a three-dimensional in vitro angiogenesis assay was evaluated. Subsequently, since vascular endothelial growth factor (VEGF) and nitric oxide (NO) are key players in the regulation of the angiogenic process, the effect of BPA on the production of these molecules by AOC was examined. BPA (10 μmol/L) stimulated AOC growth ( p < 0.05) and VEGF production ( p < 0.05), but did not modify NO levels. Our data suggest that the endocrine-disrupting effects of BPA could also be associated with the promotion of vascular growth, thus interfering with a physiologically finely tuned process resulting from a delicate balance of numerous molecular processes. The stimulatory effects of BPA on VEGF production may have negative implications, potentially switching the balance toward uncontrolled neovascularization. Moreover, since angiogenesis is involved in several pathologies, including cancer growth and progression, potential health risks of BPA exposure should be carefully monitored. [ABSTRACT FROM AUTHOR] more...
- Published
- 2017
- Full Text
- View/download PDF
48. A Boronic Acid Conjugate of Angiogenin that Shows ROS-Responsive Neuroprotective Activity.
- Author
-
Hoang, Trish T., Smith, Thomas P., and Raines, Ronald T.
- Subjects
- *
BORONIC acids , *ANGIOGENIN , *ACTIVE oxygen in the body , *GENETICS of amyotrophic lateral sclerosis , *AMYOTROPHIC lateral sclerosis treatment - Abstract
Angiogenin (ANG) is a human ribonuclease that is compromised in patients with amyotrophic lateral sclerosis (ALS). ANG also promotes neovascularization, and can induce hemorrhage and encourage tumor growth. The causal neurodegeneration of ALS is associated with reactive oxygen species, which are also known to elicit the oxidative cleavage of carbon-boron bonds. We have developed a synthetic boronic acid mask that restrains the ribonucleolytic activity of ANG. The masked ANG does not stimulate endothelial cell proliferation but protects astrocytes from oxidative stress. By differentiating between the two dichotomous biological activities of ANG, this strategy could provide a viable pharmacological approach for the treatment of ALS. [ABSTRACT FROM AUTHOR] more...
- Published
- 2017
- Full Text
- View/download PDF
49. Characterization of immune responses in feline mammary carcinoma towards the development of improved diagnostic tools and molecular therapies
- Author
-
Nascimento, Ana Catarina Gaspar do, Ferreira, Fernando António da Costa, Ferreira, João António Augusto, and Correia, Jorge Manuel de Jesus
- Subjects
Feline mammary carcinoma ,Tumor microenvironment ,Microambiente tumoral ,Carcinoma mamário felino ,Eixo PD-1/PD-L1 ,Angiogenesis ,Angiogénese ,Linfócitos infiltrantes tumorais ,Tumor infiltrating lymphocytes ,PD-1/PD-L1 axis - Abstract
Tese de Doutoramento em Ciências Veterinárias na especialidade Ciências Biológicas e Biomédicas. Área de Morfologia e Função Feline mammary carcinoma (FMC) is one of the most common tumors in female cats, being highly malignant and leading to early metastasis. In most of the cases the diagnosis is late and often leads to mastectomy to prevent the formation of new tumors in the remaining mammary glands. Thus, the development of new diagnostic and prognostic tools and molecular therapies are crucial to improve the efficient detection and treatment of these animals. In this study, the main goals were to characterize distinct immune cell subpopulations in the tumor microenvironment (TME) of FMC and to evaluate the PD-1/PDL1 and the VEGF-A/VEGFRs pathways in serum and tissue samples of cats with mammary carcinoma, in order to suggest new diagnostic and prognostic biomarkers and novel therapies for FMC. Results showed that cats with mammary carcinoma with higher densities of stromal CD8+ tumor infiltrating lymphocytes (TILs) had longer disease-free survival and overall survival, suggesting its usefulness as a favorable and novel prognostic biomarker for FMC. Furthermore, and according to the molecular subtype, triple negative (TN) normal-like tumors showed higher densities of stromal CD3+, CD8+ and CD68+ immune cells, and lower expression of PD-L1 in TILs and cancer cells, compared to HER2-positive tumor subtype. These results suggest that HER2-positive tumors had a highly immunosuppressive TME. Additionally, results demonstrated that cats with HER2-positive and TN normal-like molecular subtypes showed higher serum PD-1, PD-L1, VEGF-A, VEGFR-1, VEGFR-2 levels than healthy controls, suggesting that these molecules might be potential biomarkers for the diagnosis of cats with these two aggressive molecular subtypes. Results also suggest that these animals were under systemic immunosuppression, and may benefit from anti-PD- 1/PD-L1 immunotherapies or anti-angiogenic agents. In sum, these results identified a novel prognostic factor for FMC, and new molecules that may serve as promising non-invasive diagnostic biomarkers for cats with HER2-positive and TN normal-like molecular subtypes. Moreover, this project may lead to the development of new drugs, in order to improve the treatment of cats with mammary carcinoma. Additionally, the data obtained support the idea that spontaneous FMC is a suitable cancer model for the study of human breast cancer. RESUMO - CARACTERIZAÇÃO DA RESPOSTA IMUNITÁRIA NO CARCINOMA MAMÁRIO FELINO PARA O DESENVOLVIMENTO DE NOVOS MÉTODOS DE DIAGNÓSTICO E TERAPIAS MOLECULARES - O carcinoma mamário felino (CMF) é um dos tumores mais comuns na gata, apresentando grande malignidade e mostrando frequente e rápida metastização. Na maioria dos casos, o diagnóstico é tardio, sendo necessário realizar mastectomia de modo a evitar a formação de novos tumores nas glândulas mamárias remanescentes. Assim, o desenvolvimento de novos métodos de diagnóstico e prognóstico e de terapias moleculares torna-se fundamental para melhorar a identificação e tratamento destes animais. Neste estudo, os principais objetivos foram: (i) a caracterização das diferentes subpopulações de células imunitárias no microambiente tumoral do CMF e (ii) a avaliação dos eixos PD-1/PDL1 e VEGF-A/VEGFRs em amostras de soro e tecidos tumorais de gatas com carcinoma mamário, de modo a encontrar novos biomarcadores de diagnóstico e de prognóstico e novos alvos terapêuticos para o CMF. Os resultados obtidos revelaram que as gatas com carcinomas mamários que apresentavam mais linfócitos T infiltrantes CD8+ localizados no estroma tinham maior tempo livre de doença e maior tempo de sobrevivência, sugerindo a utilização deste marcador como de favorável prognóstico. Por outro lado, os tumores triple negative (TN) normal-like apresentaram mais células imunitárias CD3+, CD8+ e CD68+ no estroma, e uma menor expressão de PD-L1 nos linfócitos infiltrantes e nas células tumorais, comparativamente ao subtipo HER2-positivo. Estes resultados sugerem que os tumores HER2-positivo apresentam um microambiente tumoral mais imunossupressor. Adicionalmente, os resultados obtidos também sugerem que os animais com os subtipos moleculares HER2-positivo e TN normal-like apresentam níveis séricos de PD-1, PD-L1, VEGF-A, VEGFR-1 e VEGFR-2 mais elevados do que o grupo controlo, sugerindo que estas moléculas podem ser potenciais biomarcadores de diagnóstico. Os resultados também demonstraram que estes animais apresentam uma imunossupressão sistémica, podendo beneficiar de imunoterapias anti-PD-1/PD-L1 e/ou de fármacos anti-angiogénicos. Em conclusão, os resultados apresentados nesta tese identificam um novo fator de prognóstico para o CMF e novas moléculas promissoras para o diagnóstico não invasivo de gatas com os subtipos moleculares HER2-positivo e TN normal-like, abrindo perspetivas para o desenvolvimento de novas abordagens terapêuticas. Por último, os resultados obtidos reforçam a utilidade do CMF como modelo para estudos de oncologia comparada. N/A more...
- Published
- 2022
50. Impact de Nogo-A sur les propriétés vasculogéniques des cellules endothéliales progénitrices lors de la rétinopathie induite par l’oxygène
- Author
-
Ruknudin, Pakiza, Chemtob, Sylvain, and Desjarlais, Michel
- Subjects
Revascularisation ,Endothelial progenitor cell (EPC) ,Angiogenèse ,Revascularization ,NgR1 ,Cellule endothéliale progénitrice (EPC) ,Angiogenesis ,Rétinopathie induite par l’oxygène (OIR) ,Nogo-A ,Oxygen-induced retinopathy (OIR) - Abstract
La dégénérescence vasculaire et l’incapacité l’organisme à produire des vaisseaux sanguins de façon adéquate lors d’une condition ischémique est un fait saillant des rétinopathies ischémiques telles que la rétinopathie du prématuré (ROP). La ROP demeure la principale cause de défaillance visuelle et dans les cas extrêmes, de cécité chez les nourrissons prématurés. Elle présente deux phases distinctes soit une phase initiale clef de vasooblitération (VO) rétinienne et choroïdale qui entraînent la deuxième phase de néovascularisation (NV) rétinienne désorganisée et excessive. Au cours du développement normal, la NV oculaire a recours au phénomène d’angiogenèse qui consiste en la formation de nouveaux capillaires à partir de vaisseaux préexistants et de vasculogenèse qui consiste en la formation de nouveaux capillaires à partir de cellules endothéliales progénitrices dérivées de la moelle osseuse (BM-EPCs). Cette vasculogenèse implique la mobilisation des EPCs de la moelle osseuse vers la circulation afin d’être recrutées au site de NV pour contribuer de façon directe, soit en intégrant directement les structures vasculaires pour former des néovaisseaux, ou bien de façon indirecte par leur activité paracrine en libérant différents facteurs de croissance vasculaires. Toutefois, les mécanismes moléculaires impliqués dans la dysfonction des EPCs lors de la ROP sont encore mal compris. Au cours de mon mémoire, mes travaux ont ciblé la première phase de VO rétinienne afin de promouvoir la revascularisation par une thérapie basée sur une supplémentation d’EPCs natives ou reprogrammées. Compte tenu du rôle capital des EPCs dans la NV, mon mémoire s’est d’abord intéressé au rôle de Nogo-A (une protéine de la famille de réticulon), connue pour son action anti-angiogénique, sur l'activité fonctionnelle des EPCs en condition de ROP. Pour ce faire, nous avons utilisé un modèle de rétinopathie induite par l’oxygène (OIR) simulant la ROP. L’objectif global de ce projet consiste à évaluer l’interrelation entre l’effet de l’hyperoxie (une condition clef de la ROP) sur la voie de signalisation Nogo-A et de son récepteur NgR1 sur la fonction des EPCs. Premièrement, les résultats obtenus montrent une augmentation de l’expression de Nogo-A et NgR1 chez les BM-EPCs soumis ex vivo à l’hyperoxie, mais aussi dans les EPCs extraites des rats OIR. En addition, l’augmentation de l’expression de Nogo-A/NgR1 par l’hyperoxie corrèle avec la dysfonction angiogénique des EPCs caractérisées par une diminution de leurs capacités de migration et de tubulogenèse. De façon intéressante, l’inhibition de Nogo-A (par un peptide neutralisant) améliore la capacité migratoire et tubulogénique des EPCs, et protège leur fonction contre l’hyperoxie. Également, l’inhibition de Nogo-A induit l’expression du facteur angiogénique et mobilisateur d’EPCs, SDF-1, suggérant que NgR1 régule négativement l’expression de SDF-1. Par ailleurs, nous avions également pour objectif final d’évaluer l’efficacité protectrice d’une supplémentation d’EPCs natives ou reprogrammées (Nogo-/-) pour améliorer la revascularisation rétinienne dans un modèle de rat OIR. Les résultats montrent qu’une supplémentation intrapéritonéale d’EPCs natives diminue significativement la VO rétinienne, mais que cet effet pro-angiogénique devient plus prononcé par le traitement d’EPCs préconditionnées (reprogrammées par l’inhibition de Nogo-A) chez les rats OIR. Collectivement, nos résultats démontrent que : 1) l’hyperoxie cause une dysfonction angiogénique des BM-EPCs en induisant Nogo-A ce qui contribue à la VO rétinienne chez les rats OIR, et que 2) une supplémentation d’EPCs conditionnées (reprogrammées par l’inhibition de Nogo-A) est plus efficace qu’une supplémentation d’EPCs natives pour améliorer la réparation vasculaire rétinienne. Pour conclure, nous mettons donc en évidence une cible potentielle qui est la protéine Nogo-A afin de préserver l’activité biologique des EPCs et ultimement, l’intégrité vasculaire chez les rats OIR., Vascular degeneration and the inability of the body to produce adequate blood vessels during an ischemic condition is a salient feature of ischemic retinopathies such as retinopathy of prematurity (ROP). ROP remains the leading cause of visual impairment and in extreme cases, blindness in premature infants. It presents two distinct phases: a key initial phase of retinal and choroidal vasoobliteration (VO) which leads to the second phase of disorganized and excessive retinal neovascularization (NV). During normal development, ocular NV uses the phenomenon of angiogenesis which consists of the formation of new capillaries from pre-existing vessels and vasculogenesis which consists of the formation of new capillaries from progenitor endothelial cells derived from the marrow bone (BM-EPCs). This vasculogenesis involves the mobilization of EPCs from the bone marrow to the circulation in order to be recruited at the NV site to contribute directly, either by directly integrating the vascular structures to form new vessels, or indirectly by their paracrine activity by releasing different vascular growth factors. However, the molecular mechanisms involved in the dysfunction of EPCs during ROP are still poorly understood. During my thesis, my work targeted the first phase of retinal VO in order to promote revascularization by therapy based on supplementation of native or reprogrammed EPCs. Given the capital role of EPCs in NV, my thesis was first interested in the role of Nogo-A (a protein of the reticulon family), known for its anti-angiogenic action, on the functional activity of EPCs in ROP condition. To do this, we used an oxygen-induced retinopathy (OIR) model simulating ROP. The overall objective of this project is to assess the interrelationship between the effect of hyperoxia (a key condition of ROP) on the Nogo-A signaling pathway and its NgR1 receptor on the function of EPCs. First, the results obtained show an increase in the expression of Nogo-A and NgR1 in BM-EPCs subjected to hyperoxia ex vivo, but also in EPCs extracted from OIR rats. In addition, the increase in the expression of Nogo-A / NgR1 by hyperoxia correlates with the angiogenic dysfunction of EPCs characterized by a decrease in their capacity for migration and tubulogenesis. Interestingly, inhibition of Nogo-A (by a neutralizing peptide) improves the migratory and tubulogenic capacity of EPCs, and protects their function against hyperoxia. Also, inhibition of Nogo-A induces expression of the angiogenic and mobilizing factor of EPCs, SDF-1, suggesting that NgR1 negatively regulates the expression of SDF-1. In addition, our final objective was also to evaluate the protective efficacy of supplementation of native or reprogrammed EPCs (Nogo - / -) to improve retinal revascularization in an OIR rat model. The results show that intraperitoneal supplementation of native EPCs significantly decreases retinal VO, but that this pro-angiogenic effect becomes more pronounced by treatment of preconditioned EPCs (reprogrammed by inhibition of Nogo-A) in OIR rats. Collectively, our results demonstrate that: 1) hyperoxia causes angiogenic dysfunction of BM-EPCs by inducing Nogo-A which contributes to retinal VO in OIR rats, and that 2) supplementation of conditioned (reprogrammed by inhibition of Nogo-A) is more effective than supplementation of native EPCs in improving retinal vascular repairs. To conclude, we therefore highlight a potential target which is the Nogo-A protein in order to preserve the biological activity of EPCs and ultimately, vascular integrity in OIR rats. more...
- Published
- 2022
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.