1. Preclinical evaluation of Zanthoxylum piperitum Benn., traditional muscle pain remedy, for joint inflammation.
- Author
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Yun, Seung-Won, Seo, Young-Jin, Kwon, Jeong Eun, Park, Dae Won, Lee, Yeong-Geun, Choe, Tae Hwan, Kim, Seul-Ki, Lee, Hak Sung, Kim, Hyunggun, and Kang, Se Chan
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COLLAGEN , *NARCOTICS , *INTERLEUKINS , *CLINICAL drug trials , *MYALGIA , *IN vivo studies , *JOINT diseases , *INFLAMMATION , *ANIMAL experimentation , *ANALGESICS , *FORMALDEHYDE , *ORAL drug administration , *ANTIRHEUMATIC agents , *TREATMENT effectiveness , *RHEUMATOID arthritis , *ASIAN medicine , *IMMUNOLOGICAL adjuvants , *ACETIC acid , *TUMOR necrosis factors , *DRUG development , *PLANT extracts , *MICE - Abstract
Zanthoxylum piperitum has been used as a traditional Asian medicine to treat hypertension, stroke, bruise and muscle pain. It has been known to induce detoxification; affect anti-bacterial, anti-oxidant, and tyrosinase activity; inhibit osteosarcoma proliferation; anti-osteoarthritis inflammation. In this study, we aim to identify the therapeutic effect of Z. piperitum 90% EtOH extract (ZPE-LR) on rheumatoid arthritis. We investigated the anti-rheumatoid arthritis and -immunomodulatory activities of the ZPE-LR in collagen-induced arthritic (CIA) mice, a rheumatoid arthritis animal model. In order to assess the analgesic effects of ZPE-LR in vivo , acetic acid injection, formaldehyde injection, hot plate model was used. The mechanism for anti-inflammatory activity of ZPE-LR was identified with LPS-stimulated Raw 264.7 cells. Pharmacologically, oral administration of ZPE-LR into CIA mice resulted in a significant and dose-dependent decrease in clinical arthritis score and paw swelling compared to untreated negative control. Pathologic examination showed that ZPE-LR prevented morphological change in cartilage and destruction of phalanges in CIA mice. This protective effect was associated with reduced pain, inflammatory mediators such as NO, TNF-α, IL-1β, and IL-6, as well as COX-2 and iNOS expression. Furthermore, reduction of phosphor-ERK and BDNF indicates a novel rheumatoid arthritis-regulating mechanism by ZPE-LR treatment. These data suggest that the administration of ZPE-LR remarkably inhibited CIA progression and might be helpful in suppressing inflammation and pain in rheumatoid arthritis. [Display omitted] • ZPE-LR have been effectively inhibited on rheumatoid arthritis-induced pain and inflammation. • ZPE-LR prevented morphological change in cartilage and destruction of phalanges in CIA mice on pathologic examination. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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