Search

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T19:07:06Z No. of bitstreams: 1 S0037-86822011000600020.pdf: 3038690 bytes, checksum: 35ccc2486bebedea060b6e3fb1146b75 (MD5) Made available in DSpace on 2013-08-22T19:07:06Z (GMT). No. of bitstreams: 1 S0037-86822011000600020.pdf: 3038690 bytes, checksum: 35ccc2486bebedea060b6e3fb1146b75 (MD5) Previous issue date: 2011-12-01 Made available in DSpace on 2013-09-30T18:23:19Z (GMT). No. of bitstreams: 2 S0037-86822011000600020.pdf: 3038690 bytes, checksum: 35ccc2486bebedea060b6e3fb1146b75 (MD5) S0037-86822011000600020.pdf.txt: 42373 bytes, checksum: 30711f7a26c3aef34bd96bcf04bbc6af (MD5) Previous issue date: 2011-12-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:34:01Z No. of bitstreams: 2 S0037-86822011000600020.pdf: 3038690 bytes, checksum: 35ccc2486bebedea060b6e3fb1146b75 (MD5) S0037-86822011000600020.pdf.txt: 42373 bytes, checksum: 30711f7a26c3aef34bd96bcf04bbc6af (MD5) Made available in DSpace on 2014-05-20T13:34:01Z (GMT). No. of bitstreams: 2 S0037-86822011000600020.pdf: 3038690 bytes, checksum: 35ccc2486bebedea060b6e3fb1146b75 (MD5) S0037-86822011000600020.pdf.txt: 42373 bytes, checksum: 30711f7a26c3aef34bd96bcf04bbc6af (MD5) Previous issue date: 2011-12-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) INTRODUÇÃO: As abelhas africanizadas (AHBs) migraram do Brasil em 1956 para todo o continente Americano. Apesar de produtivas, são agressivas causando acidentes fatais. O objetivo foi avaliar pacientes atendidos no Hospital das Clínicas da Faculdade de Medicina de Botucatu (HC-FMB) e propor um roteiro de tratamento. MÉTODOS: Entre 2005 e 2006, foram analisados os aspectos clínicos e laboratoriais de 11 pacientes e anatomopatológicos de um que foi a óbito em 2003. RESULTADOS: A idade dos pacientes variou entre 5 e 87 com média de 42,5 anos. Sete eram do sexo masculino e quatro do feminino. O número de picadas variou entre 20 e 500. Nove deles receberam mais de 50 picadas. Os principais sinais e sintomas foram dor local, náuseas, taquicardia e vômitos. Os exames hematológicos mostraram leucocitose, neutrofilia, anemia e desvio à esquerda escalonado. Os exames bioquímicos revelaram níveis elevados de creatinofosfoquinase, desidrogenase lática e aspartato/alanina aminotransferase. O paciente que foi a óbito 24h após o atendimento tinha 11 anos, era do sexo masculino e foi atacado ao adentrar um edifício de dois andares recebendo mais de 1.000 picadas. O exame anatomopatológico mostrou lesões eritemato-purpúricas, além de necrose nos locais das picadas. Apresentou também rabdomiólise, necroses focais do miocárdio, degeneração hidrópica acompanhada de necrose tubular renal aguda, mioglobinúria e necrose centrolobular no fígado. CONCLUSÕES: Os pacientes acometidos por múltiplas picadas necessitam de tratamento imediato e por não dispormos de um soro específico desenvolvemos um roteiro que inclui os primeiros socorros, as drogas a serem empregadas e a retirada dos ferrões corretamente. INTRODUCTION: In 1956, Africanized honeybees (AHB) migrated from Brazil to other regions of the Western Hemisphere, including South, Central, and North America, except for Canada. Despite being productive, they are highly aggressive and cause fatal accidents. This study aimed to evaluate patients at the Clinical Hospital of Botucatu Medical School (HC-FMB) and to propose treatment guidelines. METHODS: From 2005 to 2006, the clinical and laboratorial aspects of 11 patients (7 male and 4 female) and the anatomopathological aspects of one patient who had died in 2003 were analyzed. RESULTS: The age of the surviving patients varied from 5 to 87 years, with a mean of 42.5 years. The majority of accidents occurred in the afternoon, and the number of stings ranged from 20 to 500. The principal signs and symptoms were pain and local inflammatory signs, nausea, tachycardia, and vomiting. Biochemical findings presented increased levels of creatine phosphokinase, lactate dehydrogenase, and aspartate/alanine aminotransferase. An 11-year-old male patient died upon entering the attic of a two-storey building where he was attacked by a swarm, receiving more than 1,000 stings. He was sent to HC-FMB where he was treated, but he died 24h later. Observed at the autopsy were erythematous-purpuric skin lesions besides necrosis at the sting locations, rhabdomyolysis, focal myocardial necrosis, tubular hydropic degeneration and focal tubular acute necrosis of the kidneys, myoglobinuria, and centrolobular necrosis in the liver. CONCLUSIONS: Accidents caused by multiple AHB stings always constitute a medical emergency. As there is no specific antivenom, we have developed guidelines, including first aid, drugs, and the proper removal of stingers. Universidade Estadual Paulista Faculdade de Medicina de Botucatu Departamento de Doenças Tropicais e Diagnóstico por Imagem Universidade Estadual Paulista Faculdade de Medicina de Botucatu Departamento de Dermatologia e Radioterapia Universidade Estadual Paulista Faculdade de Medicina de Botucatu Departamento de Pediatria Universidade Estadual Paulista Faculdade de Medicina de Botucatu Departamento de Patologia Universidade Estadual Paulista Centro de Estudos de Venenos e Animais Peçonhentos Universidade Estadual Paulista Faculdade de Medicina de Botucatu Departamento de Doenças Tropicais e Diagnóstico por Imagem Universidade Estadual Paulista Faculdade de Medicina de Botucatu Departamento de Dermatologia e Radioterapia Universidade Estadual Paulista Faculdade de Medicina de Botucatu Departamento de Pediatria Universidade Estadual Paulista Faculdade de Medicina de Botucatu Departamento de Patologia Universidade Estadual Paulista Centro de Estudos de Venenos e Animais Peçonhentos FAPESP: 2006/55545-8 FAPESP: 2007/05159-7

Universidade Estadual Paulista, Departamento de Cirurgia e Anestesiologia Veterinaria, Faculdade de Medicina Veterinaria e Zootecnia de Botucatu

Hereditary equine regional dermal asthenia belongs to a group of inherited, congenital connective tissue dysplasias usually described as hyperelastosis cutis, cutaneous asthenia, dermatosparaxis, or Ehlers-Danlos-like syndrome. This report presents the clinical and histological features of three related Quarter horses affected with regional dermal asthenia. These horses had bilateral asymmetric lesions of the trunk and lumbar regions, where the skin was hyperextensible. Handling of the skin elicited a painful response and superficial trauma led to skin wounds. The skin was thinner than normal in the affected areas, with thickened borders and harder fibrotic masses (pseudotumours). The histopathological findings included thinner and smaller collagen fibrils, and a loose arrangement of collagen fibres in the middle, adventitial and deep dermis. Masson's trichrome and Calleja stains did not reveal any abnormality of collagen and elastic fibres. Electron microscopy showed no abnormalities. As in human patients, pseudotumour histopathological findings included fibroplasia and neovascularization. The pedigree chart of these animals supports an autosomal recessive type of inheritance, which has been suggested by other studies. This is the first report of this disease in Brazil. Its clinical and histological features resemble those described in horses affected with this condition in the United States.

This report describes a case of cutaneous pythiosis in a 6-year-old female mixed breed dog, from the central west region of São Paulo State, Brazil. The cytological and histopathological analyses showed an intense inflammatory infiltrate with presence of numerous hyphal elements, suggesting infection due to Pythium insidiosum. The diagnosis was confirmed by nested-PCR, which was carried out with specific primers derived from the ribosomal DNA region. The pathogen occurs in Brazil and veterinarians should be aware of the importance of correctly diagnosing this disease and differentiating it from other fungal diseases.

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:58:17Z No. of bitstreams: 1 S0102-86502012000400010.pdf: 413885 bytes, checksum: 12b1c7e37e34fa549fdcc02d5f9eedab (MD5) Made available in DSpace on 2013-08-22T18:58:17Z (GMT). No. of bitstreams: 1 S0102-86502012000400010.pdf: 413885 bytes, checksum: 12b1c7e37e34fa549fdcc02d5f9eedab (MD5) Previous issue date: 2012-04-01 Made available in DSpace on 2013-09-30T19:53:51Z (GMT). No. of bitstreams: 2 S0102-86502012000400010.pdf: 413885 bytes, checksum: 12b1c7e37e34fa549fdcc02d5f9eedab (MD5) S0102-86502012000400010.pdf.txt: 25370 bytes, checksum: 02f3f0186a55643caefc3c74fde5697b (MD5) Previous issue date: 2012-04-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:31:57Z No. of bitstreams: 2 S0102-86502012000400010.pdf: 413885 bytes, checksum: 12b1c7e37e34fa549fdcc02d5f9eedab (MD5) S0102-86502012000400010.pdf.txt: 25370 bytes, checksum: 02f3f0186a55643caefc3c74fde5697b (MD5) Made available in DSpace on 2014-05-20T13:31:57Z (GMT). No. of bitstreams: 2 S0102-86502012000400010.pdf: 413885 bytes, checksum: 12b1c7e37e34fa549fdcc02d5f9eedab (MD5) S0102-86502012000400010.pdf.txt: 25370 bytes, checksum: 02f3f0186a55643caefc3c74fde5697b (MD5) Previous issue date: 2012-04-01 OBJETIVO: Avaliar o efeito da N-acetilcisteína na proteção renal contra lesão de isquemia/reperfusão, quando administrada logo após a indução anestésica, em ratos anestesiados com isoflurano. MÉTODOS: Dezoito ratos Wistar machos pesando mais que 300g foram anestesiados com isoflurano. A jugular interna direita e a carótida esquerda foram dissecadas e canuladas. Os animais foram distribuídos aleatoriamente em GAcetil, recebendo N-acetilcisteína por via intravenosa, 300mg/kg, e GIsot, solução salina. Foi realizada nefrectomia direita e clampeamento da artéria renal esquerda por 45 min. Os animais foram sacrificados após 48h, sendo colhidas amostras sanguíneas após a indução anestésica e ao sacrifício dos mesmos para avaliar a creatinina sérica. Realizou-se histologia renal. RESULTADOS: A variação da creatinina foi 2,33mg/dL ± 2,21 no GAcetil e 4,38mg/dL ± 2,13 no GIsot (p=0,074). Dois animais apresentaram necrose tubular intensa no GAcetil, comparados a cinco no GIsot. Apenas GAcetil apresentou animais livres de necrose tubular (dois) e degeneração tubular (um). CONCLUSÃO: Após isquemia/reperfusão renais, os ratos aos quais se administrou N-acetilcisteína apresentaram menor variação na creatinina sérica e lesões renais mais leves que o grupo controle. PURPOSE: To evaluate the effect of N-acetylcysteine, as a renoprotective agent, when administered early after anesthesia induction, against ischemia/reperfusion injury in rats anesthetized with isoflurane. METHODS: Eighteen male Wistar rats weighing > 300g were anesthetized with isoflurane. The internal jugular vein and the left carotid artery were dissected and cannulated. The animals were randomly divided into GAcetyl, receiving intravenous N-acetylcysteine, 300mg/kg, and GIsot, isotonic saline. After 30 minutes, right nephrectomy was performed and the left renal artery was clamped during 45 minutes. The animals were sacrificed after 48 hours and blood samples were taken after anesthetic induction and upon sacrificing of the animals to evaluate blood creatinine. The kidneys were sent for histological analysis. RESULTS: The variation in serum creatinine was 2.33mg/dL ± 2.21 in GAcetyl and 4.38mg/dL ± 2.13 in GIsot (p=0.074). Two animals presented intense tubular necrosis in GAcetyl, compared to 5 in GIsot. Only GAcetyl presented animals free of tubular necrosis (two) and tubular degeneration (one). CONCLUSION: After renal ischemia/reperfusion, the rats which were given N-acetylcysteine presented less variation in serum creatinine and milder kidney injuries than the control group. UNESP BSM Postgraduate Program in Anesthesiology UNESP BSM Department of Anesthesiology UNESP BSM Department of Pathology UNESP BSM Postgraduate Program in Anesthesiology UNESP BSM Department of Anesthesiology UNESP BSM Department of Pathology

The effects of Vimang®, an aqueous extract of the stem bark of Mangifera indica L. (Anacardiaceae), on cell migration in an experimental model of asthma was investigated. In vivo treatment of Toxocara canis-infected BALB/c mice for 18 days with 50 mg/kg Vimang® reduced eosinophil migration into the bronchoalveolar space and peritoneal cavity. Also, eosinophil generation in bone marrow and blood eosinophilia were inhibited in infected mice treated with Vimang®. This reduction was associated with inhibition of IL-5 production in serum and eotaxin in lung homogenates. In all these cases the effects of Vimang® were more selective than those observed with dexamethasone. Moreover, Vimang® treatment is not toxic for the animals, as demonstrated by the normal body weight increase during infection. These data confirm the potent anti-inflammatory effect of Vimang® and support its potential use as an alternative therapeutic drug to the treatment of eosinophilic disorders including those caused by nematodes and allergic diseases.

The resistance of the abdominal aorta of rats after 6, 7 and 8 weeks of malnutrition, compared with control animals, was evaluated by longitudinal tensiometry. Weakness of this vessel in malnourished rats was demonstrated; microscopic examination of the aorta stained by Masson, Calleja and hematoxylin-eosin methods showed a decrease in amorphous ground substance and an increase in the width of elastic laminae. There was no visible alteration either in the endothelial lining layer or in the smooth muscle fibers. Such alterations of the aorta are, to the authors' knowledge, the first reported modifications in the peripheral vasculature after malnutrition.