280 results on '"*CELLULAR pathology"'
Search Results
2. New Cancer Cells Apoptosis Agents: Fluorinated Aza-Heterocycles.
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Prima, D. O., Baev, D. S., Vorontsova, E. V., Frolova, T. S., Bagryanskaya, I. Yu., Slizhov, Yu. G., Tolstikova, T. G., Makarov, A. Yu., and Zibarev, A. V.
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CANCER cells , *CELLULAR pathology , *APOPTOSIS , *EPIDERMOID cancers of mucous membranes , *SQUAMOUS cell carcinoma - Abstract
Fluorinated benzo-fused 1,3-diazoles, 1,2,3-triazoles, 1,2,5-thia/selenadiazoles and 1,4-diazines were synthesized and tried for cytotoxicity towards the Hep2 (laryngeal epidermoid carcinoma) cells. The diazoles, triazoles and selenadiazoles were cytotoxic with IC50 = 2.2-26.4 μM and induced the cells apoptosis at concentrations C = 1-25 μM. At the same time, they were nontoxic towards normal cells. Due to this, these scaffolds were used in the computer-aided molecular design of new antitumor agents. Particularly, novel 1,2,3-triazole and 1,3-diazole derivatives for the binding site of the PAS domain of the transcription factor HIF were designed and some of them synthesized for further study. Overall, new anticancer agents featuring apoptotic activity are suggested. [ABSTRACT FROM AUTHOR]
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- 2017
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3. Dietary canolol induces apoptosis in human cervical carcinoma HeLa cells through ROS-MAPK mediated mitochondrial signaling pathway: In vitro and in vivo.
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Xia, Xiaoyang, Xiang, Xia, Huang, Fenghong, Zheng, Mingming, Zhang, Zhen, and Han, Ling
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CERVICAL cancer , *APOPTOSIS , *CANCER cells , *CELLULAR pathology , *CANCER cell enzymes - Abstract
Abstract Canolol (4-vinylsyringol), extracted form crude canola oil, is the promising drug toward cancer prevention and treatment. The current studies focus on the role of COX-2 signaling pathway in canolol-induced apoptosis in cancer cells. It is still unknown whether mitochondria and MAPK signaling pathways are involved. To elucidate the roles of above signaling pathways in canolol-induced apoptosis in cancer cells, human cervical carcinoma cell line HeLa and HeLa xenograft tumor model are adopted. Canolol induced apoptosis of HeLa cells and inhibited tumor growth with low systemic adverse effect, accompanying with excess generation of intracellular ROS and lysosome rupture. The results in vitro and in vivo confirmed that MAPK signaling pathways mediated mitochondrial signaling pathway activation were involved in canolol-induced apoptosis. In conclusion, these data showed that canolol induced apoptosis in HeLa cells through ROS-MAPK mediated mitochondrial signaling pathway, providing a view of the potential application of canolol as an anticancer agent. Highlights • Canolol inhibited cell survival and induced apoptosis in HeLa cells. • Canolol activated MAPK and mitochondrial signaling pathways in HeLa cells. • Canolol had less cytotoxicity in vitro and low systemic adverse effect in vivo. [ABSTRACT FROM AUTHOR]
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- 2019
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4. Proteases: History, discovery, and roles in health and disease.
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Bond, Judith S.
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PROTEOLYTIC enzymes , *HYDROLASES , *CELL proliferation , *APOPTOSIS , *CANCER cells , *CELLULAR pathology - Abstract
The Journal of Biological Chemistry (JBC) has been a major vehicle for disseminating and recording the discovery and characterization of proteolytic enzymes. The pace of discovery in the protease field accelerated during the 1971-2010 period that Dr. Herb Tabor served as the JBC's editor-in-chief. When he began his tenure, the fine structure and kinetics of only a few proteases were known; now thousands of proteases have been characterized, and over 600 genes for proteases have been identified in the human genome. In this review, besides reflecting on Dr. Tabor's invaluable contributions to the JBC and the American Society for Biochemistry and Molecular Biology (ASBMB), I endeavor to provide an overview of the extensive history of protease research, highlighting a few discoveries and roles of proteases in vivo. In addition, metalloproteinases, particularly meprins of the astacin family, will be discussed with regard to structural characteristics, regulation, mechanisms of action, and roles in health and disease. Proteases and protein degradation play crucial roles in living systems, and I briefly address future directions in this highly diverse and thriving research area. [ABSTRACT FROM AUTHOR]
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- 2019
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5. Anti-colorectal cancer effects of anti-p21Ras scFv delivered by the recombinant adenovirus KGHV500 and cytokine-induced killer cells.
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Liu, Fang-Rui, Bai, Shuang, Feng, Qiang, Pan, Xin-Yan, Song, Shu-Ling, Fang, Hong, Cui, Jing, and Yang, Ju-Lun
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PROTEIN metabolism , *ANIMAL experimentation , *APOPTOSIS , *BIOLOGICAL models , *CELL lines , *CELL physiology , *COLON tumors , *GENE therapy , *GENES , *GENETIC techniques , *IMMUNITY , *IMMUNIZATION , *IMMUNOGLOBULINS , *MICE , *GENETIC mutation , *CELLULAR pathology , *PROTEINS , *VIRUS diseases , *VIRUSES , *TREATMENT effectiveness , *MONONUCLEAR leukocytes , *CHEMICAL inhibitors ,RECTUM tumors - Abstract
Background: Colorectal cancer (CRC) is the most common type of gastrointestinal cancer. CRC gene therapy mediated by adenovirus holds great promise for the treatment of malignancies. However, intravenous delivery of adenovirus exhibits limited anti-tumor activity in vivo when used alone.Methods: In this study, the antitumor activity of the recombinant adenovirus KGHV500 was assessed with the MTT, TUNEL, Matrigel invasion and cell migration assays. To enhance the intravenous delivery of KGHV500 in vivo, cytokine-induced killer (CIK) cells were used as a second vector to carry KGHV500. We explored whether CIK cells could carry the recombinant adenovirus KGHV500 containing the anti-p21Ras single chain fragment variable antibody (scFv) gene into tumors and enhance antitumor potency.Results: Our results showed that KGHV500 exhibited significant antitumor activity in vitro. In the nude mouse SW480 tumor xenograft model, the combination of CIK cells with KGHV500 could induce higher antitumor activity against colorectal cancer in vivo than that induced by either CIK or KGHV500 alone. After seven days of treatment, adenovirus and scFv were detected in tumor tissue but were not detected in normal tissues by immunohistochemistry. Therefore, KGHV500 replicates in tumors and successfully expresses anti-p21Ras scFv in a colorectal cancer xenograft model.Conclusions: Our study provides a novel strategy for the treatment of colorectal cancer by combining CIK cells with the recombinant adenovirus KGHV500 which carried anti-p21 Ras scFv. [ABSTRACT FROM AUTHOR]- Published
- 2018
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6. PERK inhibition attenuates the abnormalities of the secretory pathway and the increased apoptotic rate induced by SIL1 knockdown in HeLa cells.
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Capone, Vanessa, Clemente, Emanuela, Sperduti, Samantha, Sallese, Michele, Pietrangelo, Laura, Protasi, Feliciano, Restelli, Elena, Chiesa, Roberto, Di Campli, Antonella, and Ornaghi, Francesca
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AUTOPHAGY , *APOPTOSIS , *NUCLEOTIDES , *PROTEIN folding , *CELLULAR pathology - Abstract
Loss-of-function mutations in the SIL1 gene are linked to Marinesco-Sjögren syndrome (MSS), a rare multisystem disease of infancy characterized by cerebellar and skeletal muscle degeneration. SIL1 is a ubiquitous adenine nucleotide exchange factor for the endoplasmic reticulum (ER) chaperone BiP. The complexity of mechanisms by which loss of SIL1 causes MSS is not yet fully understood. We used HeLa cells to test the hypothesis that impaired protein folding in the ER due to loss of SIL1 could affect secretory trafficking, impairing the transport of cargoes essential for the function of MSS vulnerable cells. Immunofluorescence and ultrastructural analysis of SIL1-knocked-down cells detected ER chaperone aggregation, enlargement of the Golgi complex, increased autophagic vacuoles, and mitochondrial swelling. SIL1-interefered cells also had delayed ER-to-plasma membrane transport with retention of Na + /K + -ATPase and procollagen-I in the ER and Golgi, and increased apoptosis. The PERK pathway of the unfolded protein response was activated in SIL1-interfered cells, and the PERK inhibitor GSK2606414 attenuated the morphological and functional alterations of the secretory pathway, and significantly reduced cell death. These results indicate that loss of SIL1 is associated with alterations of secretory transport, and suggest that inhibiting PERK signalling may alleviate the cellular pathology of SIL1 -related MSS. [ABSTRACT FROM AUTHOR]
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- 2018
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7. Therapeutic opportunities based on caspase modulation.
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Fulda, Simone
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CASPASES , *CYSTEINE proteinases , *DISEASE progression , *CANCER cells , *CELLULAR pathology - Abstract
Abstract Caspases are a family of proteolytic enzymes that play a critical role in the regulation of programmed cell death via apoptosis. Activation of caspases is frequently impaired in human cancers, contributing to cancer formation, progression and therapy resistance. A better understanding of the molecular mechanisms regulating caspase activation in cancer cells is therefore highly important. Thus, targeted modulation of caspase activation and apoptosis represents a promising approach for the development of new therapeutic options to elucidate cancer cell death. [ABSTRACT FROM AUTHOR]
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- 2018
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8. Stattic enhances the anti-proliferative effect of docetaxel via the Bax/Bcl-2/cyclin B axis in human cancer cells.
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Mohammadian, Jamal, Molavi, Ommoleila, Pirouzpanah, Mohammad Bagher, Rahimi, Ali Akbar Rahim, and Samadi, Nasser
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CANCER cells , *DOCETAXEL , *CANCER chemotherapy , *CELLULAR pathology , *ALKALOIDS - Abstract
We investigated the effects of Stattic, an important signal transducer and activator of transcription 3 (STAT3) inhibitor, on enhancing the anti-proliferative and apoptotic effects of docetaxel in lung A549 and prostate cancer DU145 cells. Cell proliferation, cellular apoptosis and expression of STAT3 target genes were evaluated by DAPI staining, Annexin V/PI staining, cell cycle analysis and Real Time PCR. The anticancer effects of docetaxel and Stattic combination therapy induced synergistic effects in A549 and DU145 cells with combination indices of 0.71 and 0.52, respectively. Annexin V/PI demonstrated that there was a 2-fold increase in the proportion of apoptotic cells in cells treated with docetaxel and Stattic in A549 and DU145 cell lines, compared with control groups. Compared with cells treated with either docetaxel or Stattic alone, the results of the current study identified a significant decrease in the transcript levels of the anti-apoptotic proteins Bcl-2 and Bcl-xl and a marked increase in the level of the pro-apoptotic protein, Bax in cells that underwent combination therapy with docetaxel and Stattic combination (P < 0.05). These results suggest that combination of a STAT3 inhibitor and taxan derivatives may be developed as a promising therapeutic strategy to treat patients with lung and prostate cancer. [ABSTRACT FROM AUTHOR]
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- 2018
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9. Novel carbazole sulfonamide microtubule-destabilizing agents exert potent antitumor activity against esophageal squamous cell carcinoma.
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Niu, Fangfei, Liu, Yonghua, Jing, Zongpan, Han, Gaijing, Sun, Lianqi, Yan, Lu, Zhou, Lanping, Wu, Yanbin, Xu, Yang, Hu, Laixing, and Zhao, Xiaohang
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CANCER cells , *CELLULAR pathology , *FIBROBLASTS , *CELL proliferation , *CYTOPLASM - Abstract
Esophageal squamous cell carcinoma (ESCC) is one of the most common cancers worldwide due to its chemoresistance and poor prognosis. Currently, there is a lack of effective small molecule drugs for the treatment of ESCC. Microtubules are an attractive target for cancer therapy since they play a central role in various fundamental cell functions. We investigated the anti-ESCC activity and mechanisms of the small molecule tubulin ligands, SL-3-19 and SL-1-73, which are two carbazole sulfonamide derivatives, in vitro and in vivo for the first time. These drugs were previously screened from a small molecule library with over 450 compounds and optimized for high aqueous solubility [1,2]. Here, we reveal the promising activities of these compounds against esophageal cancer. Mechanistically, both SL-3-19 and SL-1-73 inhibited ESCC cell growth by inducing cell apoptosis and arresting the cell cycle at G2/M phase in a dose-dependent manner. These drugs effectively inhibited microtubule assembly, greatly disrupted microtubule maturation by down-regulating acetylated α-tubulin, and significantly disrupted the vascular structure by obstructing the formation of capillary-like tubes in vitro. Consistent with their in vitro activities, SL-3-19 and SL-1-73 inhibited the growth of ESCC xenografts and inhibited the microvessel density in vivo. In summary, SL-3-19 and SL-1-73 are novel microtubule-destabilizing agents that have a potential antitumor effect on ESCC both in vitro and in vivo, and SL-3-19 had a higher activity than SL-1-73, with a low IC50 value and an observable antitumor activity in vivo. These results indicate that SL-3-19 may be a new therapeutic candidate for ESCC treatment. [ABSTRACT FROM AUTHOR]
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- 2018
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10. Mechanical cell competition.
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Brás-Pereira, Catarina and Moreno, Eduardo
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CELLULAR pathology , *CELL migration , *APOPTOSIS , *CELL culture , *GENE expression - Abstract
Maintenance of tissue organization is crucial to ensure normal organ function and organism viability. Tissues are loaded with the ability to sense the available space, measuring cell density and adapting their behaviour accordingly. To keep homeostasis, compression pressure generated by local cell density increment triggers cell elimination. During mechanical cell competition, winner cells compress the neighbouring cells, promoting tissue crowding, which leads to cell elimination. Thus, the hypersensitivity to crowding may confer a loser status, whereas resistance to mechanical-induced elimination may favour a winner status. Here we analyse the emerging field of mechanical cell competition, describing the mechanotransducers implicated in cell elimination. Furthermore, we highlight the dual role of mechanical cell competition (MCC) as tumour suppression or expansion mechanisms. [ABSTRACT FROM AUTHOR]
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- 2018
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11. Protective effect of 3-hydroxybutyrate against endoplasmic reticulum stress-associated vascular endothelial cell damage induced by low glucose exposure.
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Soejima, Eri, Ohki, Tsuyoshi, Kurita, Yayoi, Yuan, Xiaohong, Tanaka, Kayo, Kakino, Satomi, Hara, Kento, Nakayama, Hitomi, Tajiri, Yuji, and Yamada, Kentaro
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3-Hydroxybutyric acid , *ENDOPLASMIC reticulum , *HYPOGLYCEMIA , *CELLULAR pathology , *KETONES , *ENDOTHELIAL cells - Abstract
Aims/Hypothesis: The aim of this study was to elucidate the mechanism by which severe hypoglycemia accelerates vascular complications. Furthermore, we assessed the possible protective effect of ketone bodies against the endothelial cell damage caused by glucose deficiency. Methods: Human umbilical vein endothelial cells (HUVECs) were cultured at a glucose level of either 0.56 or 5.6 mmol/L with or without 3-hydroxybutyrate (3-HB) supplementation. Cell viability was assessed with a CCK-8 assay and a lactate dehydrogenase (LDH) release assay. The activity of caspases was measured using fluorogenic substrates. The expression of genes associated with endothelial cell function and endoplasmic reticulum (ER) stress was evaluated by real-time quantitative PCR. Protein levels of ER stress-related molecules were assessed by Western blotting. Results: Culture of HUVECs in low-glucose medium for 24 or 48 h resulted in reduction of cell viability accompanied by activation of caspase-3/7 and caspase-8. The addition of a pan caspase inhibitor attenuated the cell death. After incubation in the low-glucose medium, we found reduced mRNA and protein levels of endothelial nitric oxide synthase. ER stress responses mediated by phosphorylation of protein kinase RNA-like ER kinase (PERK) and cleavage of activating transcription factor 6 (ATF6) were augmented, but X-box binding protein 1 (Xbp1) splicing was reduced. Most of these responses to glucose deficiency were significantly attenuated by supplementation with 3-HB. Conclusions/Interpretation: These observations showed that exposure to low glucose induces ER stress, caspase activation, endothelial cell dysfunction and cell death. The beneficial effects of 3-HB shown in this study suggest that hypoketonemic severe hypoglycemia induced by insulin injections or insulin secretagogue administration may be more harmful than hyperketonemic severe hypoglycemia. [ABSTRACT FROM AUTHOR]
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- 2018
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12. Heme oxygnease-1 induction by methylene blue protects RAW264.7 cells from hydrogen peroxide-induced injury.
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Zhang, Xiao-tong, Sun, Xue-qiang, Wu, Chen, Chen, Jun-liang, Yuan, Jia-jia, Pang, Qing-feng, and Wang, Zhi-ping
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APOPTOSIS , *CELL death , *CANCER cells , *CELLULAR pathology , *REACTIVE oxygen species - Abstract
Although methylene blue (MB) has showed strong antioxidant effect, its effect related with heme oxygenase-1 (HO-1) is still unclear. Thus, we investigated the effects of MB on HO-1 protein content and enzyme activity, and its protective effect against hydrogen peroxide (H 2 O 2 )-induced oxidative damage in RAW264.7 macrophage. The cell viability and the release of lactate dehydrogenase of RAW264.7 were determined. The mitochondrial functions were valuated through these indexes: content of adenosine triphosphate, superoxide dismutase, concentration of reactive oxygen species and mitochondrial membrane potential. Meanwhile, high content screening tested generation of ROS, MMP and intracellular concentration of calcium ion. qRT-PCR valuated macrophage phenotype markers expression. Lastly, flow cytometry and caspase-3 detection analyzed RAW264.7 apoptosis. Our data showed that (1) Both pretreatment and posttreatment of MB increased HO-1 protein content and enzyme activity; (2) MB rescued cells from H 2 O 2 -induced mitochondrial dysfunction; (3) High content screening revealed that MB alleviated the changes including generation of reactive oxygen species, mitochondrial membrane potential and intracellular concentration of calcium ion in H 2 O 2 exposed RAW264.7; (4) MB attenuated H 2 O 2 -induced apoptosis; (5) MB pretreatment decreased the expression of M1 macrophage markers ( Tnf and Nos2 ) while increasing the expression of M2 macrophage markers ( Mrc1 and Il10 ); (6) The beneficial effect of MB was abolished by zinc protoporphyrin IX (HO-1 activity inhibitor) or HO-1 siRNA. In summary, MB protects RAW264.7 cells from H2O2-induced injury through up-regulation HO-1. [ABSTRACT FROM AUTHOR]
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- 2018
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13. Overexpression of Lymphocyte Antigen 6 Complex, Locus E in Gastric Cancer Promotes Cancer Cell Growth and Metastasis.
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Lv, Yan, Song, Yu, Ni, Chen, Wang, Shaokai, Chen, Zhipeng, Shi, Xiaojing, Jiang, Qin, Cao, Cong, and Zuo, Yun
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LYMPHOCYTES , *CANCER cells , *ANTIGENS , *CELL growth , *METASTASIS , *STOMACH cancer , *CELLULAR pathology - Abstract
Background/Aims: Lymphocyte antigen 6 complex, locus E (LY6E) is a member of the lymphostromal cell membrane Ly6 superfamily protein. The present study investigated the clinical significance and potential biological function of LY6E in gastric cancer (GC). Methods: LY6E mRNA and protein expressions in human GC tissues and GC cells were tested. Relationship between LY6E expression and the GC patients’ clinicopathologic characteristics was analyzed. LY6E was silenced by siRNA in the cultured GC cells. Results: The RNA expression microarray profiling assay results demonstrated that LY6E mRNA was significantly increased in multiple human GC tumor tissues. Immunohistochemistry (IHC) staining analysis revealed that 59 of 75 (78.7%) GC specimens were LY6E positive. LY6E over-expression in human GC was correlated with the histology grade, AJCC stage, N classification, lymphatic invasion, and tumor location. Notably, functional LY6E expression was also detected in AGS and other established GC cell lines. LY6E knockdown by targeted-siRNA inhibited AGS cell survival and proliferation. Meanwhile, the LY6E siRNA induced G1-S cell cycle arrest and apoptosis in AGC cells. Additionally, AGC cell migration was also inhibited by LY6E knockdown. Expressions of tumor-suppressing proteins, including PTEN (phosphatase and tensin homolog) and E-Cadherin, were increased in LY6E-silenced AGS cells. Conclusion: LY6E over-expression in GC is potentially required for cancer cell survival, proliferation and migration. [ABSTRACT FROM AUTHOR]
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- 2018
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14. miR-200c regulates endothelin-1 induced PASMCs abnormal proliferation and apoptosis.
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Yuan, Chao, Xu, Min, Rong, Rong, Mei, Yong, Cai, Wenyan, Li, Lin, Xue, Yao, Zhu, Baoli, Sun, Kai, and Han, Lei
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HYPERTENSION , *CARDIOVASCULAR diseases , *APOPTOSIS , *CELL death , *CELLULAR pathology - Abstract
miR-200c is an antioncogene in multiple tumors. However, its function in the pathogenesis of pulmonary arterial hypertension (PAH) has not been thoroughly investigated nor understood. In this study, we discovered that miR-200c was able to substantially upregulate in pulmonary arterial smooth muscle cells (PASMCs) treated with endothelin-1 (ET-1). miR-200c also induced cell proliferation and suppressed cell apoptosis in PASMCs in vitro. However, miR-200c had no effect on G1/S/G2 transitions during the cell cycle. Furthermore, we identified miR-200c as a new regulator of the microtubule associated protein 2 (MAP-2) and zinc finger E-box binding homeobox1 (ZEB-1) in PASMCs. miR-200c inhibited MAP-2 and ZEB-1 expression by directly binding to their 3′-untranslated regions(3′UTR) according to luciferase assay results. Our findings provide novel insights into the mechanisms of PAH pathogenesis and potential molecular biomarkers for PAH diagnosis and treatment. © 2017 IUBMB Life, 69(11):877-886, 2017 [ABSTRACT FROM AUTHOR]
- Published
- 2017
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15. Type II cGMP‑dependent protein kinase inhibits the migration, invasion and proliferation of several types of human cancer cells.
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MIN WU, YAN WU, HAI QIAN, YAN TAO, JI PANG., YING WANG, and YONGCHANG CHEN
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PROTEIN kinases , *CANCER cells , *CELL proliferation , *CANCER cell growth , *CELLULAR pathology - Abstract
Previous studies have indicated that type II cyclic guanosine monophosphate (cGMP)‑dependent protein kinase (PKG II) could inhibit the proliferation and migration of gastric cancer cells. However, the effects of PKG II on the biological functions of other types of cancer cells remain to be elucidated. Therefore, the aim of the present study was to investigate the effects of PKG II on cancer cells derived from various types of human tissues, including A549 lung, HepG2 hepatic, OS‑RC‑2 renal, SW480 colon cancer cells and U251 glioma cells. Cancer cells were infected with adenoviral constructs coding PKG II (Ad‑PKG II) to up‑regulate PKG II expression, and treated with 8‑(4‑chlorophenylthio) (8‑pCPT)‑cGMP to activate the kinase. A Cell Counting kit 8 assay was used to detect cell proliferation. Cell migration was measured using a Transwell assay, whereas a terminal deoxynucleotidyl transferase 2'‑deoxyuridine, 5'‑triphosphate nick‑end labeling assay was used to detect cell apoptosis. A pull‑down assay was used to investigate the activation of Ras‑related C3 botulinum toxin substrate (Rac) 1 and western blotting was used to detect the expression of proteins of interest. The present results demonstrated that EGF (100 ng/ml, 24 h) promoted the proliferation and migration of cancer cells, and it suppressed their apoptosis. In addition, treatment with EGF enhanced the activation of Rac1, and up‑regulated the protein expression of proliferating cell nuclear antigen, matrix metalloproteinase (MMP)2, MMP7 and B‑cell lymphoma (Bcl)‑2, whereas it down‑regulated the expression of Bcl‑2‑associated X protein. Transfection of cancer cells with Ad‑PKG II, and PKG II activation with 8‑pCPT‑cGMP, was identified to counteract the effects triggered by EGF. The present results suggested that PKG II may exert inhibitory effects on the proliferation and migration of various types of cancer cells. [ABSTRACT FROM AUTHOR]
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- 2017
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16. RNAi mediated gene silencing of ITPA using a targeted nanocarrier: Apoptosis induction in SKBR3 cancer cells.
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Charbgoo, Fahimeh, Behmanesh, Mehrdad, Nikkhah, Maryam, and Kane, Eric G
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CANCER treatment , *DNA replication , *MUTAGENESIS , *CARCINOGENESIS , *CANCER cells , *CELLULAR pathology - Abstract
A pure nucleotide pool is required for high-fidelity DNA replication and prevention of carcinogenesis in living cells. Human inosine triphosphatase ( ITPase), encoded by the ITPA gene, plays a critical role in maintaining the purity of the cellular nucleotide pool by excluding nucleotides that enhance mutagenesis. ITPase is a nucleoside triphosphate pyrophosphatase that hydrolyzes the non-canonical nucleotides inosine triphosphate ( ITP) and xanthine triphosphate ( XTP). The monophosphate products of ITPase reactions are subsequently excluded from the nucleotide pool and the improper substitution of ITP and XTP into DNA and RNA is prevented. Previous studies show that deficiency in ITPA can suppress cellular growth and enhance DNA instability. In this study, we evaluated the influence of effective ITPA down-regulation on the induction of apoptosis in a human cancer cell line using folate-single wall nanotubes ( SWNT) as a targeted nanocarrier. We assessed whether SWNT enhances IPTA-si RNA transfection efficiency in cancer cells using folate as a homing device. Since folate receptor is considerably overexpressed in cancer cells, conjugation of SWNTs to folate could enhance their cancer-specific penetrance. We found that nanocarrier mediated ITPA-si RNA transfection into SKBR3 cells caused significant reduction of ITPA mRNA expression level and complete down-regulation of the ITPase protein product. The silencing of ITPA led to promotion of apoptosis in SWNT-treated SKBR3 cancer cells. [ABSTRACT FROM AUTHOR]
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- 2017
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17. Illuminating Superoxide Anion and pH Enhancements in Apoptosis of Breast Cancer Cells Induced by Mitochondrial Hyperfusion Using a New Two-Photon Fluorescence Probe.
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WenZhang, XinWang, PingLi, HaibinXiao, WeiZhang, HuiWang, and BoTang
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SUPEROXIDES , *APOPTOSIS , *CANCER cells , *CELLULAR pathology , *BREAST cancer - Abstract
Mitochondrial morphology regulated by fusion and fission processes determines mitochondrial function and cell fate. Some studies showed hyperfused mitochondria could induce apoptosis in cancer cells, but the relevant molecular mechanisms remain elusive. Superoxide (O2·-) and pH play vital roles in mitochondrial dysfunction and apoptosis. Therefore, it is worthwhile to explore if there is an intimate relationship between mitochondrial hyperfusion and simultaneous changes in O2·- and pH levels, which will be helpful to uncover relevant detailed mechanism. For this purpose, we have developed a new reversible two-photon fluorescent probe (CFT) to simultaneously monitor O2·- and pH in 4T1 cells and mice using dual-color imaging. With the assistance of probe, we found that inhibition of Dynamin-related protein 1 (Drp1) could transduce a signal through mitochondrial complexes I and II to enhance the O2·- and pH levels and eventually induced mitohyperfusion and apoptosis in breast cancer cells. Together, these data indicate that CFT provides a robust tool for unveiling the roles of O2·- and pH in signals associated with mitochondrial dysfunction in cells and in vivo. [ABSTRACT FROM AUTHOR]
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- 2017
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18. MICU1 Alleviates Diabetic Cardiomyopathy Through Mitochondrial Ca2+-Dependent Antioxidant Response.
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Lele Ji, Fengzhou Liu, Zhe Jing, Qichao Huang, Ya Zhao, Haiyan Cao, Jun Li, Chun Yin, Jinliang Xing, Fei Li, Ji, Lele, Liu, Fengzhou, Jing, Zhe, Huang, Qichao, Zhao, Ya, Cao, Haiyan, Li, Jun, Yin, Chun, Xing, Jinliang, and Li, Fei
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DIABETIC cardiomyopathy , *DIABETES complications , *MITOCHONDRIAL pathology , *CELLULAR pathology , *PHYSIOLOGICAL effects of antioxidants , *CALCIUM metabolism , *CELL metabolism , *REACTIVE oxygen species , *ANIMAL experimentation , *APOPTOSIS , *CALCIUM-binding proteins , *CELL culture , *COENZYMES , *ECHOCARDIOGRAPHY , *GENETIC techniques , *GLUTATHIONE , *HYPERGLYCEMIA , *IMMUNOHISTOCHEMISTRY , *MICE , *MITOCHONDRIA , *POLYMERASE chain reaction , *RATS , *WESTERN immunoblotting , *METABOLISM - Abstract
Diabetic cardiomyopathy is a major cause of mortality in patients with diabetes, but specific strategies for preventing or treating diabetic cardiomyopathy have not been clarified yet. MICU1 is a key regulator of mitochondrial Ca2+ uptake, which plays important roles in regulating mitochondrial oxidative phosphorylation and redox balance. To date, however, the significance of MICU1 in diabetic hearts has not been investigated. Here, we demonstrate that MICU1 was downregulated in db/db mouse hearts, which contributes to myocardial apoptosis in diabetes. Importantly, the reconstitution of MICU1 in diabetic hearts significantly inhibited the development of diabetic cardiomyopathy, as evidenced by enhanced cardiac function and reduced cardiac hypertrophy and myocardial fibrosis in db/db mice. Moreover, our in vitro data show that the reconstitution of MICU1 inhibited the apoptosis of cardiomyocytes, induced by high glucose and high fat, through increasing mitochondrial Ca2+ uptake and subsequently activating the antioxidant system. Finally, our results indicate that hyperglycemia and hyperlipidemia induced the downregulation of MICU1 by inhibiting Sp1 expression in diabetic cardiomyocytes. Collectively, our findings provide the first direct evidence that upregulated MICU1 preserves cardiac function in diabetic db/db mice, suggesting that increasing the expression or activity of MICU1 may be a pharmacological approach to ameliorate cardiomyopathy in diabetes. [ABSTRACT FROM AUTHOR]
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- 2017
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19. Anthelmintic drug albendazole arrests human gastric cancer cells at the mitotic phase and induces apoptosis.
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XUAN ZHANG, JING ZHAO, XIANGYANG GAO, DONGSHENG PEI, and CHAO GAO
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ALBENDAZOLE , *BENZIMIDAZOLES , *APOPTOSIS inducing factor , *CELLULAR pathology , *MITOSIS - Abstract
As microtubules have a vital function in the cell cycle, oncologists have developed microtubule inhibitors capable of preventing uncontrolled cell division, as in the case of cancer. The anthelmintic drug albendazole (ABZ) has been demonstrated to inhibit hepatocellular, ovarian and prostate cancer cells via microtubule targeting. However, its activity against human gastric cancer (GC) cells has remained to be determined. In the present study, ABZ was used to treat GC cells (MKN-45, SGC-7901 and MKN-28). A a CCK-8 cell proliferation assay was performed to assess the effects of ABZ on cell viability and cell cycle changes were assessed using flow cytometry. SGC-7901 cells were selected for further study, and flow cytometry was employed to determine the apoptotic rate, immunofluorescence analysis was employed to show changes of the microtubule structure as well as the subcellular localization and expression levels of cyclin B1, and western blot analysis was used to identify the dynamics of microtubule assembly. The expression levels of relevant proteins, including cyclin B1 and Cdc2, the two subunits of mitosis-promoting factor as well as apoptosis-asociated proteins were also assessed by western blot analysis. The results showed that ABZ exerted its anti-cancer activity in GC cell lines by disrupting microtubule formation and function to cause mitotic arrest, which is also associated with the accumulation of cyclin B1, and consequently induces apoptosis. [ABSTRACT FROM AUTHOR]
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- 2017
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20. Bufalin Inhibits hTERT Expression and Colorectal Cancer Cell Growth by Targeting CPSF4.
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Zhang, Ningning, Xie, Yunpeng, Tai, Yidi, Gao, Yingying, Guo, Wei, Yu, Wendan, Li, Jia, Feng, Xu, Hao, Jiaojiao, Gao, Yue, Zhao, Xinrui, Liao, Yina, Jiang, Wei, Liu, Ge, Deng, Wuguo, and Cui, Xiaonan
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CANCER cells , *CELLULAR pathology , *COLON cancer , *APOPTOSIS , *GROWTH factors - Abstract
Background/Aims: Bufalin can induce apoptosis in certain human cancer cell lines, but bufalin has not yet been thoroughly evaluated in colorectal cancer cells. Cleavage and polyadenylation specific factor 4 (CPSF4) and human telomerase reverse transcriptase (hTERT) play important roles in colorectal cancer growth. The aim of this study was to investigate the roles and interactions of bufalin, CPSF4 and hTERT and the effects of bufalin in human colorectal cancer. Methods: We treated LoVo and SW620 cells with bufalin to investigate the effect of bufalin on proliferation, apoptosis and migration. We verified the relationship between CPSF4 and hTERT using pulldown assays, luciferase reporter assays and chromatin immunoprecipitation (ChIP) assays. Results: Bufalin inhibited the proliferation and migration of and induced apoptosis in LoVo and SW620 cells. We identified CPSF4 as an hTERT promoter-binding protein in colorectal cancer cells. Conclusion: Our study identified bufalin as a potential small molecule inhibitor for cancer therapy. [ABSTRACT FROM AUTHOR]
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- 2016
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21. Tricarbonylrhenium(i) complexes with 2-acetylpyridine-derived hydrazones are cytotoxic to NCI-H460 human large cell lung cancer.
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Garcia, Camila Vargas, Parrilha, Gabrieli Lessa, Rodrigues, Bernardo Lages, Teixeira, Sarah Fernandes, de Azevedo, Ricardo Alexandre, Ferreira, Adilson Kleber, and Beraldo, Heloisa
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CANCER cell culture , *CELLULAR pathology , *APOPTOSIS , *CELL death , *APOPTIN - Abstract
Complexes [ReCl(CO)3(H2AcPh)] (1), [ReCl(CO)3(H2AcpClPh)]·0.5C7H8 (2) and [ReCl(CO)3(H2AcpNO2Ph)]·0.5C7H8 (3) were obtained with 2-acetylpyridine-phenylhydrazone (H2AcPh) and its para-chlorophenylhydrazone (H2AcpClPh) and para-nitrophenylhydrazone (H2AcpNO2Ph) analogues. Coordination to tricarbonylrhenium(i) resulted in a higher antiproliferative activity against NCI-H460 human large cell lung cancer. Complexes (2) and (3) induced apoptosis on NCI-H460 cells. Complex (2) induced mitochondrial damage while treatment with 3 showed a later response, suggesting that probably the same effect would be observed at higher concentrations or longer treatments. Both complexes (2) and (3) showed a high antioxidant activity, 2 being more potent in reducing intracellular reactive oxygen species (ROS) production. [ABSTRACT FROM AUTHOR]
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- 2016
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22. Apoptosis and Necrosis of Human Breast Cancer Cells by an Aqueous Extract of Euphorbia hirta leaves.
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Behera, Bandana, Dash, Jyoshna, Pradhan, Debasish, Tripathy, Gitanjali, and Pradhan, Rakesh
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APOPTOSIS inducing factor , *CANCER cells , *CELLULAR pathology , *BREAST cancer , *TUMOR budding - Abstract
Introduction: Traditional medicines for mammary tumour are unreasonable and have genuine symptoms. Non-ordinary normal medications have increased wide acknowledgment because of their assurance of a cure with negligible or no symptoms, however minimal experimental confirmation exists. One such basic cure is the leaves of the Euphorbia hirta plant. Methods: It is initially reported utilization of the fluid concentrate of Euphorbia hirta leaves breast cancer cells. The capacity of the concentrate to impel apoptosis and corruption in the human bosom malignancy cell line MCF-7, contrasted with typical human skin fibroblasts (MDA MB-231), was dictated by morphological changes in the cells utilizing light microscopy, DNA fragmentation, and brilliant stains (Annexin V and Propidium Iodide) utilizing Flow Cytometry and fluorescent microscopy. Results: Apoptosis was instigated in both cells, and more in MCF-7, when they were treated with 25% and half concentrate, while rot was watched mostly after presentation to raised concentrate fixations (75%). DNA discontinuity came about for both cells, in a period and dosage subordinate way. Both cells, at all concentrate fixations, demonstrated no critical contrasts in the quantity of living, dead, apoptotic, and necrotic cells. Conclusion: At long last, the outcomes might show that apoptotic changes in MCF-7 might be free of caspase-3, which is included in apoptosis and is inadequate in MCF-7 cells. [ABSTRACT FROM AUTHOR]
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- 2016
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23. Cytotoxic amounts of cisplatin induce either checkpoint adaptation or apoptosis in a concentration-dependent manner in cancer cells.
- Author
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Swift, Lucy H. and Golsteyn, Roy M.
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ALKYLATING agents , *APOPTIN , *PROGRAMMED cell death 1 receptors , *CELLULAR pathology , *CISPLATIN - Abstract
Background Information Checkpoint adaptation (entry into mitosis with damaged DNA) is a process that links arrest at the G2/M cell cycle checkpoint and cell death in cancer cells. It is not known, however, whether cells treated with the genotoxic agent, cisplatin, undergo checkpoint adaptation or if checkpoint adaptation is a major pathway leading to cell death or not. Therefore, we investigated the relationship between treatment with cisplatin and cytotoxicity in cancer cells. Results Treatment of HT-29 human colorectal adenocarcinoma cells with cisplatin can induce cell death by one of two different mechanisms. Cells treated with a cytotoxic 30 μM amount of cisplatin died after undergoing checkpoint adaptation. Before dying, however, almost all treated cells were positive for histone γH2AX staining and contained high levels of cyclin B1. Rounded cells appeared that were positive for phospho-Ser10 histone H3, with low levels of phospho-Tyr15 cyclin-dependent kinase 1, high levels of cyclin-dependent kinase 1 activity, and checkpoint kinase 1 that was not phosphorylated on Ser345. These cells were in mitosis with damaged DNA. Strikingly, with 30 μM cisplatin, 81% of cells had entered mitosis before dying. By contrast, after treatment with 100 μM cisplatin, nearly all cells died but only 7% of cells had entered mitosis. Instead, these cells died by apoptosis; they were positive for annexin-V staining, contained cleaved caspase 3, cleaved caspase 9 and cleaved PARP and did not contain Mcl-1. Conclusions Our data demonstrate that cancer cells treated with cisplatin can undergo one of two modes of cell death depending upon concentration used. These findings suggest that checkpoint adaptation is likely a primary pathway in genotoxic cell death at pharmacological concentrations of cisplatin. Significance Checkpoint adaptation might be a common biochemical pathway taken by human cancer cells in response to pharmacologically relevant, cytotoxic amounts of damaged DNA. [ABSTRACT FROM AUTHOR]
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- 2016
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24. Effects of a novel carbocyclic analog of pyrrolo[2,3-d]pyrimidine nucleoside on pleiotropic induction of cell death in prostate cancer cells with different androgen responsiveness.
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Suh, Hyewon, Choi, Ko-woon, Lee, Jongbok, Ryou, Chongsuk, Rhee, Hakjune, and Lee, Chul-Hoon
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CANCER cells , *CELLULAR pathology , *PLEIOTROPHIN , *ANDROGENESIS , *REPRODUCTION - Abstract
Prostate cancer is the most frequently diagnosed cancer and is one of the leading causes of male cancer death in the world. Recently, in the course of our screening for a novel anticancer compound, we synthesized carbocyclic analogs of pyrrolo[2,3- d ]pyrimidine nucleoside; compounds 5 , and 6 . In the current study, we report the effects of compound 5 on pleiotropic induction of cell death via up-regulation of AR-associated p21 Cip1 protein in prostate cancer cells with different androgen responsiveness, such as LNCaP (androgen-dependent and -sensitive), LNCaP C4-2 (androgen-independent and -sensitive; androgen-refractory), and DU145 (androgen-independent and -insensitive) cells. The treatment of LNCaP cells with 6 μM compound 5 for 24 h stimulated the androgen receptor (AR) activity and dramatically up-regulated transcription (56-fold) of p21 Cip1 , which, in turn, induces typical apoptosis in the cells. However, induction of apoptosis through up-regulation (23-fold) of AR-associated p21 Cip1 achieved in LNCaP C4-2 cells was possible by intensive cell treatment with compound 5 (9 μM, 48 h), because the cells are less sensitive and independent to androgen than LNCaP cells. Furthermore, 6 μM compound 5 -treated DU145 cells, which exhibit extremely low AR activation due to no androgen responsiveness and dependency, showed neither up-regulation of p21 Cip1 nor apoptotic induction. Instead, a different type of cell death, autophagy-like death through the LC3B-associated autophagosome formation, was obviously induced in DU145 cells. Taken together, our results suggest that pleiotropic induction of prostate cancer cell death by compound 5 is determined by how efficiently and how abundantly androgen-dependent activation of the AR occurs, whereas compound 6 shows no induction of apoptosis in LNCaP cells. [ABSTRACT FROM AUTHOR]
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- 2016
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25. Apoptotic events induced by prototype foamy virus infection.
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Kim, Jinsun, Hamid, Faysal Bin, and Shin, Cha-Gyun
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VIRUS diseases , *MEDICAL microbiology , *CELLULAR pathology , *NUCLEAR fragmentation , *MEDICINE - Abstract
Foamy virus infection induces cytopathology in several cell types from different species. But the exact mechanism is still unknown. In this study, we have investigated the mechanism of cell death induced by prototype foamy virus (PFV) infection in baby hamster kidney (BHK 21) cell lines. PFV induces apoptosis by exhibiting morphological alterations such as chromatin condensation, blebbing, and nuclear fragmentation. In addition, PFV infection causes chromosomal DNA fragmentation, up-regulation of Bax, and activation of caspase-3, but not caspase-8. Up-regulation of Bax initiates the translocation of cytochrome-c from mitochondria to the cytoplasm, suggesting predominantly to the mitochondrial-mediated pathway. Blocking apoptosis using caspase inhibitors increased PFV-infected BHK 21 cell viability. Although blocking apoptosis resulted in reduced progeny release, maximal accumulation of PFV was found in apoptosis-blocked cells. This report provides the first experimental evidence of apoptosis induced by PFV infection, which will provide valuable insights for foamy viral pathology. [ABSTRACT FROM AUTHOR]
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- 2016
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26. Synthesis, characterization, and antitumor properties of ruthenium(II) anthraquinone complexes.
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Wang, Jin-Quan, Zhao, Zi-Zhuo, Bo, Hua-Ben, and Chen, Qi-Zhu
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RUTHENIUM , *ANTHRAQUINONES , *REACTIVE oxygen species , *CANCER cells , *CELLULAR pathology , *DNA , *ANTINEOPLASTIC agents , *CHEMICAL synthesis - Abstract
Three new Ru(II) complexes, [Ru(dmb)2(ipad)](ClO4)2(dmb = 4,4′-dimethyl-2,2′-bipyridine, ipad = 2-(anthracene-9,10-dione-2-yl) imidazo[4,5-f][1,10]phenanthroline,1), [Ru(dmp)2(ipad)](ClO4)2(dmp = 2,9-dimethyl-1,10-phenanthroline,2), and [Ru(dip)2(ipad)](ClO4)2(dip = 4,7-diphenyl-1,10-phenanthroline,3), have been synthesized and characterized. The three Ru(II) complexes intercalate with the base pairs of DNA. Thein vitroantiproliferative activities and apoptosis-inducing characteristics of these complexes were investigated. The complexes exhibited cytotoxicity against various human cancer cell lines. BEL-7402 cells displayed the highest sensitivity to1, accounted for by the greatest cellular uptake. Complex1was shown to accumulate preferentially in the nuclei of BEL-7402 cells and cause DNA damage and induce apoptosis, which involved cell cycle arrest and reactive oxygen species generation. [ABSTRACT FROM PUBLISHER]
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- 2016
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27. Neonatal bronchopulmonary dysplasia increases neuronal apoptosis in the hippocampus through the HIF-1α and p53 pathways.
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Yin, Rong, Yuan, Lin, Ping, Lili, and Hu, Liyuan
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BRONCHOPULMONARY dysplasia , *OXYGEN toxicity , *NEONATAL diseases , *CELLULAR pathology , *APOPTOTIC bodies - Abstract
Neonatal bronchopulmonary dysplasia (BPD) might lead to an increased risk for brain injury. The present study aims to investigate the effects of neonatal BPD on neuronal apoptosis in the hippocampus and cognitive function and to explore the underlying mechanisms. The results revealed that BPD model rat pups exhibited more apoptotic cells in the hippocampus and longer escape latencies in the Morris maze test. Both the caspase-dependent and caspase-nondependent signal pathways were activated. Further examinations showed an elevated p53 level by BPD via HIF-1α induction, while the caspase-3 in the hippocampus was suppressed by both HIF-1α and p53 inhibitor. These findings suggested that neonatal BPD caused impaired cognitive function and neuron apoptosis in hippocampus via p53 and HIF-1α. Although the precise mechanism requires further investigation, this study provided new evidence for and an explanation of the impaired CNS developmental outcomes of BPD. [ABSTRACT FROM AUTHOR]
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- 2016
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28. Riproximin modulates multiple signaling cascades leading to cytostatic and apoptotic effects in human breast cancer cells.
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Pervaiz, Asim, Zepp, Michael, Adwan, Hassan, and Berger, Martin
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LARGE-breasted women , *CELLULAR pathology , *CANCER cell enzymes , *CANCER cell differentiation , *REGENERATION (Biology) - Abstract
Background: Riproximin, a type II ribosome-inactivating protein (RIP), has shown significant cytotoxic effects in diverse types of cancer cells. To better understand its therapeutic potential, elaborated investigations on the mechanistic aspects of riproximin deem crucial. In this study, we focused on riproximin-mediated changes in cellular properties and corresponding molecular pathways in breast cancer cells. Methods: Cytotoxicity of riproximin was determined by MTT assay, while the clonogenic and migratory effects were determined by colony formation, migration, and scratch assays. Cytostatic and apoptotic effects were studied by flow cytometry and nuclear staining procedures. Alterations at molecular levels were scrutinized by means of microarray and qRT-PCR methodologies. Results: Riproximin induced significant cytotoxic effects in the selected human breast cancer cells MDA-MB-231 and MCF-7. Profound inhibition of migration and colony formation were observed in both cell lines in response to riproximin exposure. Concomitantly, a significant arrest in S phase and nuclear fragmentation were observed as causes for its cytostatic and apoptotic effects, respectively. Genetic profiling revealed pronounced induction of the anticancer cytokine IL24/MDA-7 and ER-stress-related GADD genes. In addition, prominent inhibition of the genes relevant to migration (RHO GTPases), anti-apoptotic activities (BCL family), and cell cycle (cyclins) was also noticed. Conclusion: Riproximin, with its significant antineoplastic effects, modulates multiple cytostatic and apoptotic pathways in breast cancer cells. Results from these investigations highlight the future therapeutic potential of this naturally occurring compound for breast cancer. [ABSTRACT FROM AUTHOR]
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- 2016
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29. DNA damage and cellular abnormalities in tuberculosis, lung cancer and chronic obstructive pulmonary disease.
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Gonçalves da Silva, Andréa Lúcia, Josimara Bresciani, Maribel, Evelyn Karnopp, Thaís, Ferreira Weber, Augusto, Henrique Ellwanger, Joel, Pêgas Henriques, João Antonio, de Moura Valim, Andréia Rosane, and Gonçalves Possuelo, Lia
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DNA damage , *TUBERCULOSIS , *CELLULAR pathology , *LUNG cancer , *OBSTRUCTIVE lung diseases - Abstract
Background: Tuberculosis (TB), Lung Cancer (LC) and Chronic Obstructive Pulmonary Diseases (COPD) affect millions of individuals worldwide. Monitoring of DNA damage in pathological situations has been investigated because it can add a new dimension to clinical expression and may represent a potential target for therapeutic intervention. The aim of this study was to evaluate DNA damage and the frequency of cellular abnormalities in TB, LC and COPD patients by comparing them to healthy subjects. Methods: The detection of DNA damage by a buccal micronucleus cytome assay was investigated in patients with COPD (n = 28), LC (n = 18) and TB (n = 22) and compared to control individuals (n = 17). Results: The COPD group had a higher frequency of apoptotic cells compared to TB and LC group. The TB group showed a higher frequency of DNA damage, defect in cytokinesis, apoptotic and necrotic cells. Patients with LC had low frequency of chromosomal aberrations than TB and COPD patients. Conclusion: COPD patients showed cellular abnormalities that corresponded to cell death by apoptosis and necrosis, while patients with TB presented defects in cytokinesis and dysfunctions in DNA repair that resulted in the formation of micronucleus (MN) besides apoptotic and necrotic cells. Patients with COPD, TB and LC had a low frequency of permanent DNA damage. [ABSTRACT FROM AUTHOR]
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- 2015
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30. A cyclometalated iridium(III) complex that induces apoptosis in cisplatin-resistant cancer cells.
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Wang, Jin-Quan, Hou, Xiao-Juan, Bo, Hua-Ben, and Chen, Qi-zhu
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CANCER cells , *IRIDIUM compounds , *CELLULAR pathology , *TRANSITION metal compounds , *APOPTOSIS - Abstract
A novel cyclometalated iridium(III) complex, [Ir(CˆN) 2 (HPIP)]Cl (IrC) was synthesized and characterized. IrC exhibited about 10-fold higher cytotoxicity than cisplatin against A549 cancer cell line. Interestingly, a cisplatin-resistant cell line, A549-CP/R showed sensitivity to IrC. Further study suggested that IrC induced apoptosis via negatively regulated the nuclear factor-kappa B (NF-κB) pathway, which involved reactive oxygen species (ROS) generation, Bcl-2 and caspase family members activation. Taken together, these results suggest that IrC is able to overcome chemoresistance and may be an effective treatment for platinum-resistant cancer therapy. [ABSTRACT FROM AUTHOR]
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- 2015
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31. Xanthohumol inhibits cell cycle progression and proliferation of larynx cancer cells in vitro.
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Sławińska-Brych, Adrianna, Król, Sylwia Katarzyna, Dmoszyńska-Graniczka, Magdalena, Zdzisińska, Barbara, Stepulak, Andrzej, and Gagoś, Mariusz
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LARYNX , *CANCER cells , *RESPIRATORY organs , *CELLULAR pathology , *BIOLOGICAL rhythms - Abstract
Xanthohumol (XN), a prenylflavonoid derived from the hop plant ( Humulus lupulus L.) has been found to exhibit a broad spectrum of biological properties, including anti-cancer activity. In this study, the mechanisms involved in anti-cancer activity of XN in human RK33 and RK45 larynx cancer cell lines were investigated. The effect of XN on the viability of larynx cancer and normal cells (human skin fibroblasts HSF and rat oligodendroglia-derived cells, OLN-93) was compared. Additionally, the influence of XN on proliferation, cell cycle progression, induction of apoptosis in larynx cancer cells, as well as the molecular mechanisms underlying in these processes were analyzed. XN promoted the reduction of cell viability in cancer cells, but showed low cytotoxicity to normal cells. The decrease in cell viability in the cancer cells was coupled with induction of apoptosis via two pathways. The mechanisms involved in these effects of XN were associated with cell growth inhibition by induction of cell cycle arrest in the G 1 phase, increased p53 and p21/WAF1 expression levels, downregulation of cyclin D1 and Bcl-2, and activation of caspases-9, -8, and -3. Moreover, this compound inhibited phosphorylation of ERK1/2, suggesting a key role of the ERKs pathway in the XN-mediated growth suppressing effects against the studied cells. These results indicate that XN could be used as a potential agent for the treatment of patients with larynx cancer. [ABSTRACT FROM AUTHOR]
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- 2015
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32. Structurally related ganoderic acids induce apoptosis in human cervical cancer HeLa cells: Involvement of oxidative stress and antioxidant protective system.
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Liu, Ru-Ming, Li, Ying-Bo, Liang, Xiang-Feng, Liu, Hui-Zhou, Xiao, Jian-Hui, and Zhong, Jian-Jiang
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CANCER cells , *APOPTOSIS , *CELLULAR pathology , *CELL death , *GANODERMA - Abstract
Ganoderic acids (GAs) produced by Ganoderma lucidum possess anticancer activities with the generation of reactive oxygen species (ROS). However, the role of oxidative stress in apoptotic process induced by GAs is still undefined. In this study, the effects of four structurally related GAs, i.e. GA-T, GA-Mk, and two deacetylated derivatives of GA-T (GA-T1 and GA-T2) on the antioxidant defense system and induced apoptosis in cervical cancer cells HeLa were investigated in vitro . Our results indicated that the tested GAs (5–40 μM) induced apoptotic cell death through mitochondrial membrane potential decrease and activation of caspase-9 and caspase-3. Furthermore, GAs increased the generation of intracellular ROS and attenuated antioxidant defense system by decreasing glutathione (GSH) level, superoxide dismutase (SOD) and glutathione peroxidase (GPX) activities. The above effects were remarkably blocked by the exogenous antioxidants, i.e. N -acetylcysteine, catalase and diphenyleneiodonium chloride. The potency of the four GAs toward induced apoptosis, generation of ROS and suppression of antioxidant defense system was in the order of: GA-T > GA-Mk ≈ GA-T1 > GA-T2 in HeLa cells. These findings suggest that GAs induced mitochondria-dependent cell apoptosis in HeLa cells are mediated via enhancing oxidative stress and depressing antioxidant defense. Additionally, the acetylation of hydroxyl groups in GAs may contribute to their pro-oxidant activities and cytotoxicity, which is helpful to the development of novel chemotherapy agents. [ABSTRACT FROM AUTHOR]
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- 2015
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33. A Novel, Potent, Small Molecule AKT Inhibitor Exhibits Efficacy against Lung Cancer Cells In Vitro.
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Dinavahi, Saketh S., Prasanna, Rajagopalan, Dharmarajan, Sriram, Perumal, Yogeeswari, and Viswanadha, Srikant
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PROTEIN kinase B , *LUNG cancer , *CANCER cells , *CELLULAR pathology , *SMALL molecules - Abstract
Purpose Anomalies of Akt regulation, including overexpression in lung cancer, impart resistance to conventional chemotherapy and radiation, thereby implicating this kinase as a therapeutic intervention point. A novel scaffold of Akt inhibitors was developed through virtual screening of chemical databases available at Birla Institute of Technology and Science, Pilani, Hyderabad, based on docking studies using Maestro. A benzothienopyrimidine derivative (BIA-6) was identified as a potential lead molecule that inhibited Akt1 enzyme activity with an IC50 of 256 nM. Materials and Methods BIA-6 was tested for in vitro Akt1 inhibition using a fluorescence resonance energy transfer kit. Anti-proliferative activity was tested in NCI-H460, A549, NCI-H1975, and NCI-H2170 cell lines. The effect of the compound on p-Akt (S473) was estimated. Results BIA-6 allosterically caused a dose dependent reduction of growth of cell lines with a half maximal growth inhibition (GI50) range of 0.49 μM to 6.6 μM. Cell cycle analysis indicated that BIA-6 caused a G1 phase arrest at < 100 nM but led to apoptosis at higher doses. BIA- 6 also exhibited synergism with standard chemotherapeutic agents. Conclusion BIA-6 is a novel, allosteric Akt inhibitor with potent anti-cancer activity in lung cancer cell lines, that effectively blocks the phosphoinositide-3 kinase/Akt pathway with a high margin selectivity towards normal cells. [ABSTRACT FROM AUTHOR]
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- 2015
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34. Geraniin inhibits TGF-β1-induced epithelial–mesenchymal transition and suppresses A549 lung cancer migration, invasion and anoikis resistance.
- Author
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Ko, Hyeonseok
- Subjects
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LUNG cancer , *ANOIKIS , *CELLULAR pathology , *APOPTOSIS , *CANCER - Abstract
The epithelial–mesenchymal transition (EMT) is an important cellular process during which epithelial polarized cells become motile mesenchymal-appeared cells, which, in turn, induces the metastatic of cancer. Geraniin is a polyphenolic component isolated from Phyllanthus amarus , which exhibits a wide range of pharmacological and physiological activities, such as antitumor, anti-hyperglycemic, anti-hypertensive, antimicrobial, and antiviral activities. However, the possible role of geraniin in the EMT is unclear. We investigated the effect of geraniin on the EMT. Transforming growth factor-beta 1 (TGF-β1) induces the EMT to promote lung adenocarcinoma migration, invasion, and anoikis resistance. To understand the suppressive role of geraniin in lung cancer migration, invasion, and anoikis resistance, we investigated the use of geraniin as inhibitors of TGF-β1-induced EMT in A549 lung cancer cells in vitro. Here, we show that geraniin remarkably increased expression of the epithelial marker E-cadherin and repressed Snail upregulation and expression of the mesenchymal marker N-cadherin and vimentin during the TGF-β1-induced EMT. Geraniin also inhibited the TGF-β1-induced increase in cell migration, invasion, and anoikis resistance of A549 lung cancer cells. Additionally, geraniin markedly inhibited TGF-β1-regulated activation of Smad2. Taken together, our findings provide new evidence that geraniin suppresses lung cancer migration, invasion, and anoikis resistance in vitro by inhibiting the TGF-β1-induced EMT. [ABSTRACT FROM AUTHOR]
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- 2015
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35. Infection Profiles of Selected Aquabirnavirus Isolates in CHSE Cells.
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Gamil, Amr A. A., Evensen, Øystein, and Mutoloki, Stephen
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VIRUS diseases , *CELLULAR pathology , *APOPTOSIS , *SEROTYPES , *SEROLOGY - Abstract
The wide host range and antigenic diversity of aquabirnaviruses are reflected by the presence of a collection of isolates with different sero- and genotypic properties that have previously been classified as such. Differences in cytopathogenic mechanisms and host responses induced by these isolates have not been previously examined. In the present study, we investigated infection profiles induced by genetically and serologically closely related as well as distant isolates in-vitro. CHSE-214 cells were infected with either E1S (serotype A3, genogroup 3), VR-299 (serotype A1, genogroup 1), highly virulent Sp (TA) or avirulent Sp (PT) (serotype A2, genogroup 5). The experiments were performed at temperatures most optimum for each of the isolates namely 15°C for VR-299, TA and PT strains and 20°C for E1S. Differences in virus loads and ability to induce cytopathic effect, inhibition of protein synthesis, apoptosis, and induction of IFNa, Mx1, PKR or TNFα gene expression at different times post infection were examined. The results showed on one hand, E1S with the highest ability to replicate, induce apoptosis and IFNa gene expression while VR-299 inhibited protein synthesis and induced Mx1 and PKR gene expression the most. The two Sp isolates induced the highest TNFα gene expression but differed in their ability to replicate, inhibit protein synthesis, and induce gene expression, with TA being more superior. Collectively, these findings point towards the adaptation by different virus isolates to suit environments and hosts that they patronize. Furthermore, the results also suggest that genetic identity is not prerequisite to functional similarities thus results of one aquabirnavirus isolate cannot necessarily be extrapolated to another. [ABSTRACT FROM AUTHOR]
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- 2015
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36. Methyl Sartortuoate Inhibits Colon Cancer Cell Growth by Inducing Apoptosis and G2/M-Phase Arrest.
- Author
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Qiusheng Lan, Shoufeng Li, Wei Lai, Heyang Xu, Yang Zhang, Yujie Zeng, Wenjian Lan, and Zhonghua Chu
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APOPTIN , *APOPTOSIS inducing factor , *APOPTOTIC bodies , *PROGRAMMED cell death 1 receptors , *TURCOT syndrome , *COLON cancer , *CELLULAR pathology - Abstract
The potential anti-neoplastic activity of terpenoids is of continued interest. In this study, we investigate whether methyl sartortuoate, a terpenoid isolated from soft coral, induced cell cycle arrest and apoptosis in a human colon cancer cell line. Culture studies found that methyl sartortuoate inhibited colon cancer cell (LoVo and RKO) growth and caused apoptotic death in a concentration- and time-dependent manner, by activation of caspase-8, caspase-9, caspase-3, p53 and Bax, and inactivation of B-cell lymphoma 2 (Bcl-2) apoptosis regulating proteins. Methyl sartortuoate treatment led to reduced expression of cdc2 and up-regulated p21 and p53, suggesting that Methyl sartortuoate induced G2-M arrest through modulation of p53/p21/cdc2 pathways. Methyl sartortuoate also up-regulated phospho-JNK and phospho-p38 expression levels. This resulted in cell cycle arrest at the G2-M phase and apoptosis in LoVo and RKO cells. Treatment with the JNK inhibitor SP600125 and the p38 MAPK inhibitor SB203580 prevented methyl sartortuoate-induced apoptosis in LoVo cells. Moreover, methyl sartortuoate also prevented neoplasm growth in NOD-SCID nude mice inoculated with LoVo cells. Taken together, these findings suggest that methyl sartortuoate is capable of leading to activation of caspase-8, -9, -3, increasing p53 and Bax/Bcl-2 ratio apoptosis through MAPK-dependent apoptosis and results in G2-M phase arrest in LoVo and RKO cells. Thus, methyl sartortuoate may be a promising anticancer candidate. [ABSTRACT FROM AUTHOR]
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- 2015
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37. Pachymic acid induces apoptosis via activating ROS-dependent JNK and ER stress pathways in lung cancer cells.
- Author
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Jun Ma, Jun Liu, Chunwei Lu, and Dingfang Cai
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APOPTOSIS inducing factor , *APOPTOTIC bodies , *LUNG cancer , *CELLULAR pathology , *CANCER cell growth , *CANCER invasiveness , *CANCER cells - Abstract
Background: Pachymic acid (PA), a lanostane-type triterpenoid from Poria cocos, has been reported to possess antiemetic, anti-inflammatory, and anti-cancer properties. Nonetheless, the anti-tumor effect of PA in lung cancer cells remains unclear. Herein, we report the chemotherapeutic effects and underlying mechanisms of PA against human lung cancer. Methods: The anti-proliferative ability of PA on lung cancer cells was assessed by MTT, colony formation and EdU proliferation assays. Flow cytometric analysis was used to detect cell cycle changes. Apoptosis was determined by annexin V/PI double-staining and the DNA ladder formation assays. The expressions of the apoptosis-related proteins were analysed by western blot. The in vivo efficacy of PA was measured using a NCI-H23 xenograft model in nude mice. Results: PA exhibited anti-tumor effects in vitro accompanied by induction of G2/M phase arrest and apoptosis in NCI-H23 and NCI-H460 lung cancer cells. Mechanistically, our data showed that PA induced reactive oxygen species (ROS) production, resulting in the activation of both c-Jun N-terminal kinase (JNK) and endoplasmic reticulum (ER) stress apoptotic pathways in lung cancer cells. Moreover, blockage of ROS production reversed PA-induced JNK and ER stress activation. Finally, PA inhibited the growth of NCI-H23 xenograft tumors without causing any host toxicity, and inhibited cell proliferation and induction of apoptosis of tumor cells in tumor xenograft tissues. Conclusions: In summary, our study demonstrates that PA induces apoptosis through activation of the JNK and ER stress pathways in human lung cancer cells. Our findings provide a rationale for the potential application of PA in lung cancer therapy. [ABSTRACT FROM AUTHOR]
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- 2015
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38. Qianliening capsules influence the apoptosis of benign prostatic hyperplasia epithelial-1 cells by regulating the extracellular matrix.
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JIANHENG ZHOU, JIUMAO LIN, LIYA LIU, YUQING ZHENG, and ZHENFENG HONG
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HYPERPLASIA , *CELLULAR pathology , *APOPTOSIS , *B cells , *EXTRACELLULAR matrix - Abstract
The present study investigated whether Qianliening capsules (QC) affected the apoptosis of benign prostatic hyperplastia epithelial (BPH-1) cells by regulating the extracellular matrix (ECM). The levels of fibronectin (FN) and collagen IV were determined in the culture medium of BPH-1 cells maintained in normal medium and of BPH-1 cells maintained in an environment rich in FN and collagen IV using an enzyme-linked immunosorbent assay. Reverse transcription quantitative polymerase chain reaction and western blot analysis were performed to determine the mRNA and protein expression levels of FN, collagen IV, B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax) and cyclin D1, respectively. The cell morphology and viability were determined using light microscopy and an MTT assay and cell apoptosis was detected by annexin V staining. The results demonstrated that FN and collagen IV affected the apoptotic response of the BPH-1 cells, QC treatment significantly reduced the levels of FN and collagen IV secreted by the cells into the culture medium (P<0.01), inhibited the mRNA and protein expression levels of FN, collagen IV, Bcl-2 and cyclin D1 and promoted the mRNA and protein expression of Bax. Therefore, one of the mechanisms underlying the anti-BPH action of QC involves promoting apoptosis by regulating the expression of the extracellular matrix. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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39. Inhibitory and Apoptosis-Inducing Effects of Newcastle Disease Virus Strain AF2240 on Mammary Carcinoma Cell Line.
- Author
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Ahmad, Umar, Ahmed, Ismaila, Keong, Yong Yoke, Abd Manan, Nizar, and Othman, Fauziah
- Subjects
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ACADEMIC medical centers , *ANALYSIS of variance , *APOPTOSIS , *BREAST tumors , *CELL culture , *FLOW cytometry , *CELLULAR pathology , *RESEARCH funding , *STATISTICS , *VIRUS diseases , *DATA analysis , *DATA analysis software , *IN vitro studies ,BREAST tumor prevention - Abstract
Breast cancer is the malignant tumour that developed from cells of the breast and is the first leading cause of cancer death among women worldwide. Surgery, radiotherapy, and chemotherapy are the available treatments for breast cancer, but these were reported to have side effects. Newcastle disease virus (NDV) known as Avian paramyxovirus type-1 (APMV1) belongs to the genus Avulavirus in a family Paramyxoviridae. NDV is shown to be a promising anticancer agent, killing tumour cells while sparing normal cells unharmed. In this study, the oncolytic and cytotoxic activities of NDV AF2240 strain were evaluated on MDA-MB-231, human mammary carcinoma cell line, using MTT assay, and its inhibitory effects were further studied using proliferation and migration assays. Morphological and apoptotic-inducing effects of NDV on MD-MB-231 cells were observed using phase contrast and fluorescence microscopes. Detection of DNA fragmentation was done following terminal deoxyribonucleotide transferase-mediated Br-dUTP nick end labeling staining (TUNEL) assay, which confirmed that the mode of death was through apoptosis and was quantified by flow cytometry. Furthermore, analysis of cellular DNA content demonstrated that the virus caused an increase in the sub-G1 phase (apoptotic peak) of the cell cycle. It appears that NDV AF2240 strain is a potent anticancer agent that induced apoptosis in time-dependent manner. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
40. Porcine parvovirus infection induces apoptosis in PK-15 cells through activation of p53 and mitochondria-mediated pathway.
- Author
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Zhang, Hongling, Huang, Yong, Du, Qian, Luo, Xiaomao, Zhang, Liang, Zhao, Xiaomin, and Tong, Dewen
- Subjects
- *
PARVOVIRUS diseases , *VIRUS diseases in swine , *APOPTOSIS , *P53 protein , *MITOCHONDRIA , *CELLULAR pathology , *CELL membranes - Abstract
Porcine parvovirus (PPV) infection has been reported to induce the cytopathic effects (CPE) in some special host cells and contribute the occurrence of porcine parvovirus disease, but the molecular mechanisms underlying PPV-induced CPE are not clear. In this study, we investigated the morphological and molecular changes of porcine kidney cell line (PK-15 cells) infected with PPV. The results showed that PPV infection inhibited the viability of PK-15 cells in a time and concentration dependent manner. PPV infection induced typical apoptotic features including chromatin condensation, apoptotic body formation, nuclear fragmentation, and Annexin V-binding activity. Further studies showed that Bax was increased and translocated to mitochondria, whereas Bcl-2 was decreased in PPV-infected cells, which caused mitochondrial outer-membrane permeabilization, resulting in the release of mitochondrial cytochrome c, followed by caspase-9 and caspase-3 activation. However, the expression of Fas and Fas ligand (FasL) did not appear significant changes in the process of PPV-induced apoptosis. Moreover, PPV infection activated p53 signaling, which was involved in the activation of apoptotic signaling induced by PPV infection via regulation of Bax and Bcl-2. Taken together, our results demonstrated that PPV infection induced apoptosis in PK-15 cells through activation of p53 and mitochondria-mediated apoptosis pathway. This study may contribute to shed light on the molecular pathogenesis of PPV infection. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
41. Anti-Proliferative Effects of Siegesbeckia orientalis Ethanol Extract on Human Endometrial RL-95 Cancer Cells.
- Author
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Chi-Chang Chang, Hsia-Fen Hsu, Kuo-Hung Huang, Jing-Mei Wu, Shyh-Ming Kuo, Xue-Hua Ling, and Jer-Yiing Houng
- Subjects
- *
ETHANOL , *CELLULAR pathology , *CANCER cell enzymes , *CHEMICAL reactions , *CHEMICAL processes , *CHEMICAL energy , *CATALYSTS - Abstract
Endometrial cancer is a common malignancy of the female genital tract. This study demonstrates that Siegesbeckia orientalis ethanol extract (SOE) significantly inhibited the proliferation of RL95-2 human endometrial cancer cells. Treating RL95-2 cells with SOE caused cell arrest in the G2/M phase and induced apoptosis of RL95-2 cells by up-regulating Bad, Bak and Bax protein expression and down-regulation of Bcl-2 and Bcl-xL protein expression. Treatment with SOE increased protein expression of caspase-3, -8 and -9 dose-dependently, indicating that apoptosis was through the intrinsic and extrinsic apoptotic pathways. Moreover, SOE was also effective against A549 (lung cancer), Hep G2 (hepatoma), FaDu (pharynx squamous cancer), MDA-MB-231 (breast cancer), and especially on LNCaP (prostate cancer) cell lines. In total, 10 constituents of SOE were identified by Gas chromatography-mass analysis. Caryophyllene oxide and caryophyllene are largely responsible for most cytotoxic activity of SOE against RL95-2 cells. Overall, this study suggests that SOE is a promising anticancer agent for treating endometrial cancer [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
42. Regulation of insulin-like growth factor signaling by metformin in endometrial cancer cells.
- Author
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YA XIE, JING-LU WANG, MEI JI, ZHONG-FU YUAN, ZHENG PENG, YI ZHANG, JIAN-GUO WEN, and HUI-RONG SHI
- Subjects
- *
CANCER cells , *ENDOMETRIAL cancer , *SOMATOMEDIN , *CELLULAR pathology , *SOMATOTROPIN - Abstract
Obesity, diabetes and insulin resistance are marked risk factors that promote the development of type I endometrial cancer. Previous studies have demonstrated that insulin-like growth factor 1 (IGF-1) and IGF-2 promote cell proliferation in endometrial cancer cells, while metformin reverses this effect and inhibits cell proliferation. However, the effects of metformin on the regulation of the IGF signaling pathway are unclear. The aim of this study was to investigate the regulation of IGF signaling by metformin in endometrial cancer cells, and to determine the effects of metformin combined with IGF-1 receptor (IGF-1R) inhibitor on cell proliferation and apoptosis. Cell proliferation was assessed following exposure of Ishikawa and HEC-1B endometrial cancer cell lines to metformin and/or the IGF-1R inhibitor, PPP. Apoptosis was assessed by TdT-mediated dUTP nick end labeling assay. Metformin was observed to downregulate IGF-1R and upregulate IGF binding protein-1 (IGFBP-1) mRNA and protein expression, while compound C, an adenosine monophosphate protein kinase inhibitor, reversed this effect. Metformin administered with PPP inhibited endometrial cancer cell proliferation to a greater degree than treatment with either agent alone. At high concentrations (1 or 2 mM), metformin induced apoptosis in endometrial cancer cells. Metformin combined with IGF-1R axis inhibitors may act synergistically to kill tumor cells, as metformin was shown to delay and prevent IGF-1R feedback. In conclusion, this study supported the results of animal studies and subclinical studies, demonstrating the feasibility of metformin combined with IGF-1R axis inhibitors in the treatment of endometrial cancer. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
43. Design, Synthesis and Evaluation of N13-Substituted Evodiamine Derivatives against Human Cancer Cell Lines.
- Author
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Senchuan Song, Zhiyong Chen, Shaoxue Li, Yanmin Huang, Yiqian Wan, and Huacan Song
- Subjects
- *
CELLULAR pathology , *CELL lines , *SOLUTION (Chemistry) , *APOPTOSIS , *CELL death - Abstract
Attempting to improve the anticancer activity and solubility of evodiamine in simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) solutions, thirty-eight N13-substituted evodiamine derivatives were designed, synthesized and tested for antitumor activities against six kinds of human cancer cell lines, namely prostate cancer (DU-145 and PC-3), lung cancer (H460), breast cancer (MCF-7), colon cancer (HCT-5) and glioblastoma (SF-268). The solubility of these compounds in SGF and SIF solutions was evaluated, and apoptosis induced by 2-2, 2-3, 2-16 and 3-2 was determined. The results showed: (1) among all compounds examined, 2-16 showed the highest antitumor activity and a broader spectrum of activity, with IC50 values ranging from 1-2 μM; (2) their solubility was obviously improved; (3) 2-3, 2-16 and 3-2 had a significant impact inducing apoptosis in some cancer cell lines. The preliminary structure-activity relationships of these derivatives were discussed. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
44. Curcumin Potentiates Antitumor effect of Gemcitabine in Human Breast Cancer in vitro.
- Author
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Serasanambati, Mamatha, Chilakapati, Shanmuga Reddy, Manikonda, Pavan Kumar, and Kanala, Jagadeeswara Reddy
- Subjects
- *
BREAST cancer , *CANCER treatment , *CANCER in women , *CELL proliferation , *CELLULAR pathology - Abstract
Breast cancer is the most commonly diagnosed disease among women. In recent years, chemotherapeutic drugs such as gemcitabine, erlotinib, etc. have been developed for the treatment of breast cancer. Breast cancers relapse due to the generation of chemoresistance that makes therapeutic drugs ineffective. Hence, agents that can reduce the chemoresistance to the therapeutic drugs are being developed for better advancement of in cancer therapy. The present study evaluates the efficiency of curcumin, in suppression of gemcitabine induced NF-kB activity and in the enhancement of antitumor effect of gemcitabine on MCF-7 and MDA MB-231breast cancer cells. 10 or 20 μM of curcumin and 10 or 100 μM of gemcitabine, alone or in combination, were used. Cell proliferation by MTT assay, apoptotic effects by Live/Dead assay, nuclear factor-kB (NF-kB) activation or suppression by EMSA were determined. The results indicated a decrease in cell proliferation of up to 61% (p ⩽ 0.01) and 45% (p ⩽ 0.01) at 20 μM curcumin and 100 μM of gemcitabine in MCF- 7 and MDA MB-231, respectively. Whereas 20 μM curcumin potentiated the apoptotic effects of gemcitabine (100μM) predominantly in MCF-7 by 61% and in MDA MB-231 by 46% which was determined by using Live/Dead assay. However, curcumin (20 μM) significantly (p ⩽ 0.05) suppressed NF-kB activation by 80% which was induced by gemcitabine (100μM) in both cell lines. The data obtained from the present investigation shows the dose dependent changes in MCF-7 and MDA MB-231. The combined results revealed the beneficial role of curcumin in potentiating the anti-tumor effects of gemcitabine through NF-kB suppression and apoptotic effects. [ABSTRACT FROM AUTHOR]
- Published
- 2013
45. High levels of reactive oxygen species in gold nanoparticle-targeted cancer cells following femtosecond pulse irradiation.
- Author
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Minai, Limor, Yeheskely-Hayon, Daniella, and Yelin, Dvir
- Subjects
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CANCER cells , *FEMTOSECOND pulses , *REACTIVE oxygen species , *NANOPARTICLES , *CANCER treatment , *ANTIGEN presenting cells , *CELLULAR pathology - Abstract
Cancer cells could be locally damaged using specifically targeted gold nanoparticles and laser pulse irradiation, while maintaining minimum damage to nearby, particle-free tissue. Here, we show that in addition to the immediate photothermal cell damage, high concentrations of reactive oxygen species (ROS) are formed within the irradiated cells. Burkitt lymphoma B cells and epithelial breast cancer cells were targeted by antibody-coated gold nanospheres and irradiated by a few resonant femtosecond pulses, resulting in significant elevation of intracellular ROS which was characterized and quantified using time-lapse microscopy of different fluorescent markers. The results suggest that techniques that involve targeting of various malignancies using gold nanoparticles and ultrashort pulses may be more effective and versatile than previously anticipated, allowing diverse, highly specific set of tools for local cancer therapy. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
46. Type 1 insulin-like growth factor receptor monoclonal antibody (HX-1162) treatment for liver cancer.
- Author
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Xue-Hui Chen, Zhi-Qiang Li, Hua Peng, Su-Mei Jin, Hui-Qing Fu, Tie-Chui Zhu, and Xiao-Gang Weng
- Subjects
- *
INSULIN , *PANCREATIC secretions , *MONOCLONAL antibodies , *CELLULAR pathology - Abstract
One of the most important molecules mediating the proliferation, growth, and metastasis of cancer cells is insulin-like growth factor (IGF), with its receptor IGF-1R. Here, we describe the potential of an IGF-1R monoclonal antibody, HX-1162, on liver cancer apoptosis in vitro and in vivo. We found that HX-1162 could induce the apoptosis of cultured liver cancer cells. Additionally, HX-1162 treatment inhibited the tumor growth after cancer cell grafting and enhanced the cell apoptosis inside the tumor tissue. We conclude that IGF-1R targeting therapy provides a new avenue toward treating liver cancer. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
47. Cytopathic effects: virus-modulated manifestations of innate immunity?
- Author
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Agol, Vadim I.
- Subjects
- *
CELLULAR pathology , *NATURAL immunity , *VIRUS diseases , *VIRAL replication , *APOPTOSIS , *NECROSIS , *HOST-virus relationships , *ENVIRONMENTAL impact analysis - Abstract
The capacity to injure infected cells is a widespread property of viruses. Usually, this cytopathic effect (CPE) is ascribed to viral hijacking of cellular resources to fulfill viral needs. However, evidence is accumulating that CPE is not necessarily directly coupled to viral reproduction but may largely be due to host defensive and viral antidefensive activities. A major part in this virus–cell interaction appears to be played by a putative host-encoded program with multiple competing branches, leading to necrotic, apoptotic, and, possibly, other types of cell suicide. Manifestations of this program are controlled and modulated by host, viral, and environmental factors. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
48. Oncosis: An important non-apoptotic mode of cell death
- Author
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Weerasinghe, Priya and Buja, L. Maximilian
- Subjects
- *
CELL death , *APOPTOSIS , *IN vitro studies , *CELLULAR pathology , *NECROSIS - Abstract
Abstract: It is now increasingly accepted that apoptosis may not be the only form of cell death seen in vitro and in vivo; hence there is a need to study novel forms of cell death. The explosion of cell death research that followed the recognition of apoptosis by Kerr and colleagues in the late 1960s completely obscured the fact that apoptosis is not the only form of cell death. Apoptosis manifests itself by cell shrinkage followed by breakup; another form (oncosis) is almost the opposite: it involves cell swelling and coagulation of the cytoplasm. The name oncosis was chosen over a century ago by von Recklinghausen, a top collaborator of Rudolph Virchow and thereby one of the founders of cellular pathology. Nevertheless, oncosis was forgotten, largely because a satisfactory technique for preparing tissue sections did not exist at the time. Also confusion developed regarding the distinction between oncosis as a mode of cell injury and cell death, and necrosis as a degradation process following cell death. In this review we have described the many characteristics of oncosis from a morphological and biochemical standpoint, and we briefly examine the application of oncosis in disease processes. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
- View/download PDF
49. Chikungunya Virus Induces a More Moderate Cytopathic Effect in Mosquito Cells than in Mammalian Cells.
- Author
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Li, Yong-Gang, Siripanyaphinyo, Uamporn, Tumkosit, Uranan, Noranate, Nitchakarn, a-nuegoonpipat, atchareeya, Tao, Ran, Kurosu, Takeshi, Ikuta, Kazuyoshi, Takeda, Naokazu, and anantapreecha, Surapee
- Subjects
- *
CHIKUNGUNYA , *CELLULAR pathology , *MOSQUITOES , *MAMMALIAN cell cycle , *APOPTOSIS , *ALPHAVIRUSES - Abstract
Objectives: Chikungunya virus (CHIKV) is an alphavirus belonging to the Togaviridae family. Alphaviruses cause a chronic non-cytopathic infection in mosquito cells, while they develop a highly cytopathic infection in cells originating from various vertebrates. In this study, we compared the cytopathic effect (CPE) induced by CHIKV in Vero cells and a mosquito cell line, C6/36 cells. Methods: CPE and the virus titers were compared between the CHIKV-infected C6/36 and Vero cells. Apoptosis was measured by TUNEL assay, and the differences between the C6/36 and Vero cells were compared. Results: CHIKV infection induced strong CPE and apoptosis in the Vero cells, but light CPE in the C6/36 cells. The virus titers produced in the C6/36 cells were much higher than those produced in the Vero cells. Conclusions: The reason CHIKV induced strong CPE is that this virus triggers strong apoptosis in Vero cells compared with C6/36 cells. CHIKV established a persistent infection in C6/36 cells after being passaged 20 times. CHIKV infection in mosquito cells was distinct from that in Vero cells. The cell and species specificity of CHIKV-induced cell death implies that the cellular and viral regulators involved in apoptosis may play an important role in determining the outcome of CHIKV infection. Copyright © 2012 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
50. Clinical correlations of miR-21 expression in colorectal cancer patients and effects of its inhibition on DLD1 colon cancer cells.
- Author
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Faltejskova, Petra, Besse, Andrej, Sevcikova, Sabina, Kubiczkova, Lenka, Svoboda, Marek, Smarda, Jan, Kiss, Igor, Vyzula, Rostislav, and Slaby, Ondrej
- Subjects
- *
CANCER patients , *COLON cancer , *ORGANS (Anatomy) , *COLON (Insects) , *CELLULAR pathology , *CANCER cells - Abstract
Purpose: MicroRNA-21 (miR-21) is one of the miRNAs that are frequently and highly overexpressed in tumor tissue of colorectal cancer (CRC) patients; however, only a little is known about its functional role in CRC. Methods: We examined the expression level of miR-21 in 44 paired samples of tumoral and non-tumoral colon tissues diagnosed for CRC using TaqMan real-time PCR method. Furthermore, we used miR-21 inhibitor (anti-miR-21) to transient knockdown of miR-21 in DLD-1 colon cancer cells and examined the effects of miR-21 silencing on viability, apoptosis, chemosensitivity, cell cycle, and migration of DLD1 cells. Results: The expression levels of miR-21 were significantly increased in CRC tumor tissue ( P < 0.0001). Significant differences in miR-21 levels were observed also between CRC tissues of patients with CRC in different clinical stages: I vs. II ( P = 0.033) and I vs. IV ( P = 0.021). Kaplan-Meier analysis proved that the miR-21 expression levels are correlated to shorter overall survival of CRC patients ( P = 0.0341). MiR-21 silencing in DLD1 cell line had no effect on the cell viability; however, when combined with chemotherapeutics (5-FU, L-OHP, and SN38), it contributed to the decrease of cell viability. Suppression of miR-21 decreased cell migration ability of DLD-1 cells by nearly 30 % ( P = 0.016). Conclusion: We have confirmed the overexpression of miR-21 in CRC samples and its correlation with advanced disease and shorter overall survival. These findings could be described in part by the fact that CRC cells with increased expression of miR-21 have higher migration ability. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
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