1. Glutamine Protects against Mouse Abdominal Aortic Aneurysm through Modulating VSMC Apoptosis and M1 Macrophage Activation.
- Author
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Wang J, Da X, Chen Y, Yuan A, and Pu J
- Subjects
- Animals, Mice, Humans, Reactive Oxygen Species metabolism, Disease Models, Animal, Male, Macrophages drug effects, Macrophages metabolism, Macrophages immunology, Aorta, Abdominal pathology, Aorta, Abdominal drug effects, Matrix Metalloproteinase 9 metabolism, Matrix Metalloproteinase 2 metabolism, Tumor Necrosis Factor-alpha metabolism, Interleukin-6 metabolism, Calcium Phosphates, Aortic Aneurysm, Abdominal pathology, Aortic Aneurysm, Abdominal prevention & control, Aortic Aneurysm, Abdominal metabolism, Apoptosis drug effects, Glutamine pharmacology, Angiotensin II pharmacology, Macrophage Activation drug effects, Muscle, Smooth, Vascular drug effects, Muscle, Smooth, Vascular pathology, Muscle, Smooth, Vascular cytology, Myocytes, Smooth Muscle drug effects, Myocytes, Smooth Muscle metabolism, Oxidative Stress drug effects
- Abstract
Glutamine (Gln), known as the most abundant free amino acid, is widely spread in human body. In this study, we demonstrated the protective effects of glutamine against mouse abdominal aortic aneurysm (AAA) induced by both angiotensin II (AngII) and calcium phosphate (Ca
3 (PO4 )2 ) in vivo , which was characterized with lower incidence of mouse AAA. Moreover, histomorphological staining visually presented more intact elastic fiber and less collagen deposition in abdominal aortas of mice treated by glutamine. Further, we found glutamine inhibited the excessive production of reactive oxide species (ROS), activity of matrix metalloproteinase (MMP), M1 macrophage activation, and apoptosis of vascular smooth muscle cells (VSMCs) in suprarenal abdominal aortas of mice, what's more, the high expressions of MMP-2 protein, MMP-9 protein, pro-apoptotic proteins, and IL-6 as well as TNF-α in protein and mRNA levels in cells treated by AngII were down-regulated by glutamine. Collectively, these results revealed that glutamine protected against mouse AAA through inhibiting apoptosis of VSMCs, M1 macrophage activation, oxidative stress, and extracellular matrix degradation., Competing Interests: Competing Interests: The authors have declared that no competing interest exists., (© The author(s).)- Published
- 2024
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