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The present study was aimed at investigating the effect of inclusion of green tea leaf (GTE) extract (at 3 and 6% levels) in the icing medium employed during the chilled storage (2±1°C) of Japanese threadfin bream Nemipterus japonicus .Fish stored in normal ice (NI)was treated as control. Changes in microbiological, biochemical and sensory quality characteristics were monitored during the period of storage. Multivariate comparison was performed using principal component analysis (PCA) for the mean sensory, microbiological and chemical attributes. Samples stored in normal ice (NI) had only 8 days shelf life whereas both 3% GTE and 6% GTE stored samples had a shelf life of 16 days. From an economic point of view 3% GTE can be adopted for preservation of fish and there was no significant difference in the parameters between 3 and 6% GTE levels. Visualisation by PC plots clearly separated the investigated samples according to storage conditions, thus indicating the importance of GTEI treatment. The present study indicated that the application of GTE in ice (GTEI) is a promising technique to increase the shelf life of N.japonicus in chilled condition and hence the technique can be commercially exploited. Keywords: Chilled storage, Green tea leaf extract, Nemipterus japonicus , Principal component analysis, Shelf life

Threadfin bream ( Nemipterus japonicus ) is an important marine fish species used in the preparation of surimi. The waste generated from surimi production contains large quantity of recoverable proteins, which could be utilised for byproduct development. In this study, Isoelectric precipitation (IEP) technique as a method to recover proteins from fish processing waste was attempted. The alkali aided IEP process extracted the fish protein isolate (FPI) efficiently with a yield of 84.13±0.11%. The highest protein yield was recorded at a homogenised sample to water ratio of 1:9. Maximum protein content was found at pH 13 with 19.63 ±0.52% yield. FPI extracted had higher protein content, less ash and low lipid contents compared to the fish processing waste. The mean yield and protein composition of FPI extracted atdifferent treatments of pH and sample to water ratiowere found to be significantly different (p

This study was carried out to detect the parasites affecting Japanese threadfin bream (Nemipterus japonicus). A total of 150 specimens collected from Gulf of Suez- Red Sea in Egypt from November 2016 to April 2017. Specimens were subjected to clinical, postmortem and parasitological examinations for detection of nematode parasites. The clinical examination showed no pathognomic clinical abnormalities on the external body surface except abdominal distension. Postmortem examination revealed excessive mucus secretion and swelling in intestine. Only the endoparasite Hysterothylacium gadi (Muller, 1776) (Nematoda: Anisakidae) was recorded in the intestine and stomach of examined fish. The prevalence rate was 54.7% and the intensity of the infection ranged 1-3 worms per fish. The morphological characteristics of this species were described in details using light and scanning electron microscopy. The results showed that females are larger than males. It is also shown that the cephalic region provided with three hexagonal-shaped lips separated from each other by three prominent interlabium; ventrolateral lips somewhat smaller. Dorsal lip provided on its surface with two double papillae, whereas both ventrolateral lips with one papilla and one small papilla. Interlabila are conical and their base is wider than length (Figs. 1&4). The worm was characterized by a broad lateral alae starting immediately below lips gradually narrowing posterioly and extending as narrow alae along whole body. A groove like structure is seen in the ventral surface of the body (Fig. 9). The tail is conical and tail tips of both sexes provided with numerous conical spines and the male has equal spicules.

The population structure of three Nemipterid species (Nemipterus japonicus, Nemipterus bipunctatus and Nemipterus zysron) from the Gulf of Suez was investigated. A total of 3663 specimens (2597 N. japonicus, 576 N. bipunctatus and 490 N. zysron) were collected during the fishing season 2014/2015 in the Gulf of Suez. The maximum life span estimated from otolith reading was 5 years for N. japonicus and N. bipunctatus while, it was 4 years for N. zysron. The parameters of von Bertalanffy growth equation were L∞ = 31.66 cm, K = 0.24 year−1 and to = −1.2 year for N. japonicus; L∞ = 29.55 cm, K = 0.26 year−1 and to = −1.2 year for N. bipunctatus and were L∞ = 24.69 cm, K = 0.38 year−1 and to = −0.84 year for N. zysron. The estimated exploitation rate for the three species exceeded the optimum exploitation rate, indicating an overexploitation of the nemipterid species in the Gulf of Suez. The yield per recruit analysis revealed that the fishing pressure of the trawling fleet in the Gulf of Suez, has overridden the critical level. Findings suggest that decrease in the fishing effort of trawl fishery and increase the length of the mesh size is required for the protection of the nemipteridae resources in the Gulf. Keywords: Trawl, Age and growth, Nemipterid species, Exploitation rate, Yield per recruit

The effect of freezing and frozen storage of threadfin bream fish (Nemipterus japonicus) meat on the setting and gel-forming ability has been evaluated. The dynamic viscoelastic properties of fish ...

The mid-water multiday trawl fishery along the north-west coast of India generally targets adult largehead hairtail, Trichiurus lepturus. Bycatch landed by the commercial mid-water multiday trawlers operated from Veraval harbor during August 2017–December 2019 was investigated by analyzing catch composition with respect to its spatial and temporal patterns. A total of 834 bycatch sample observations from mid-water trawl fishery were examined. Bycatch comprises of 123 species and account for 53.36% of the total catch. Highest composition in bycatch comprises of teleosts (62.09%), cephalopods (35.88%), crustaceans (1.47%) and elasmobranchs (0.56%) respectively. Uroteuthis duvaucelii, Sepia elliptica, Nemipterus japonicus, Otolithes cuvieri, S. pharaonis, Priacanthus hamrur, Sepiella inermis and Saurida tumbil were the dominant species with high annual average catches; and together contributed 50.30% of total bycatch. Discards formed 6.32% of total catch and consisted of 62 species belonging to 29 families. The most common discards were juveniles of S. tumbil, N. japonicus, Platycephalus indicus, O. cuvieri, Johnius glaucus and T. lepturus. CPUE of bycatch differed significantly between the seasons with the lowest being in summer and highest in post-monsoon period. Minimum bycatch rates were observed in the offshore waters than inshore waters. Endangered, Threatened and Protected (ETP) species listed in CITES as well as IUCN were also observed. The present study revealed that quantities of bycatch landed off northwest coast of India in the Arabian Sea were relatively higher than that landed by mid-water trawlers operating in other parts of the world. Hence the mid-water trawl fishery operating in north-west coast of India and targeting largehead hairtail needs to be closely monitored to mitigate and reduce the quantity of bycatch landed by them.

The reproductive biology of Nemipterus japonicus (Bloch, 1791) along the north-east coast of India was studied during 2012-2015 based on samples from the trawl landings at Digha, Paradeep, Visakhapatnam and Kakinada. Adults dominated the landings. Significant contribution by females was observed in most months of the year. Size at first maturity for females was 15.1 cm. Peak spawning season was during August to February and September to March in the northern and southern regions. Maturity and gonado-somatic index (GSI) were found to correlate well with sea surface temperature (SST). Fecundity increased with length and weight and varied from 13,176 to 1,30,798. Ova diameter distribution indicated the species to be a multiple/batch spawner.

Japanese threadfin bream Nemipterus japonicus (Bloch, 1791) is among the most abundant and commercially important species in Pakistan. From the coast of Pakistan, four demersal trawl surveys in October–November 2009 and May–June, August, October and November in 2010 were carried out. The purpose of this study is to estimate the population dynamics and status of the stock of the N. japonicus from Pakistani waters based on the research trawl surveys from the research area. The data consist of n=784 length-weight pairs and n=7 530 length frequency with the maximum length and weight of 29 cm and 358 g respectively. The length frequency data were analyzed using ELEFAN method in FiSAT computer package. The parameters of length and weight relationship were b=2.778, a=0.032 and R 2=0.973. The estimated von Bertalanffy growth function parameters were L ∞=30.45 cm, K=0.270 year−1. Based on length-converted catch curve analysis the total mortality (Z) during this study was estimated at 0.960 year−1. The natural mortality coefficient (M) was 0.74 year−1 using Pauly’s equation (the annual average sea surface temperature was 27°C), therefore, the fishing mortality coefficients (F) were 0.22 year−1. The yield per recruit analysis indicated that when t c was 2, F max was estimated at 1.2 and F 0.1 at 1.1. When t c was 1, F max was estimated at 0.95 and F 0.1 at 0.8. Because current age at first capture is about 1 year and F current was 0.22, F current is smaller than F 0.1 and F max, which indicated that the fishery is about in a safe condition. When using Gulland (1971) biological reference point, F opt was equals to M (0.74). The current fishing mortality rate of 0.22 was smaller than the target biological reference point.

The focus of the study was to investigate the antioxidant activity of hydrolyzed muscle protein of Nemipterus japonicus and Exocoetus volitans. The trypsin protein hydrolysates of both fish showed maximum free radical scavenging potential and lipid peroxidation inhibition. Furthermore, it was purified by chromatographic methods followed by the lipid peroxidation inhibition; free radical scavenging assay was performed before and after purification. The purified peptide fractions of N. japonicus and E. volitans exhibited higher activity against polyunsaturated fatty acids (PUFA) peroxidation which was similar to natural antioxidants like α-tocopherol. Free radical scavenging potencies were measured by electron spin resonance technique (ESR). The purified peptide of E. volitans quenched free radicals (DPPH, hydroxyl, and superoxide) slightly more than N. japonicus. The amino acid composition of both fish protein hydrolysates showed variations in their ratio. The purified peptides were tested for cell cytotox...

Trophic interactions in commercially exploited demersal finfishes in the southeastern Arabian Sea of India were studied to understand trophic organization with emphasis on ontogenic diet shifts within the marine food web. In total, the contents of 4716 stomachs were examined from which 78 prey items were identified. Crustaceans and fishes were the major prey groups to most of the fishes. Based on cluster analysis of predator feeding similarities and ontogenic diet shift within each predator, four major trophic guilds and many sub-guilds were identified. The first guild ‘detritus feeders’ included all size groups of Cynoglossus macrostomus , Pampus argenteus , Leiognathus bindus and Priacanthus hamrur . Guild two, named ‘Shrimp feeders’, was the largest guild identified and included all size groups of Rhynchobatus djiddensis and Nemipterus mesoprion , medium and large Nemipterus japonicus , P. hamrur and Grammoplites suppositus , small and medium Otolithes cuvieri and small Lactarius lactarius . Guild three, named ‘crab and squilla feeders’, consisted of few predators. The fourth trophic guild, ‘piscivores’, was mainly made up of larger size groups of all predators and all size groups of Pseudorhombus arsius and Carcharhinus limbatus . The mean diet breadth and mean trophic level showed strong correlation with ontogenic diet shift. The mean trophic level varied from 2.2 ± 0.1 in large L. bindus to 4.6 ± 0.2 in large Epinephelus diacanthus and the diet breadth from 1.4 ± 0.3 in medium P. argenteus to 8.3 ± 0.2 in medium N. japonicus . Overall, the present study showed that predators in the ecosystem have a strong feeding preference for the sergestid shrimp Acetes indicus , penaeid shrimps, epibenthic crabs and detritus.

A total of 30 specimens (73–95 mm standard length) of Nemipterus randalli were collected along the Lebanon coast. This species, a Lessepsian migrant previously misidentified as Nemipterus japonicus, appears to be well established in the Levantine basin of the Mediterranean Sea. The small size and immature status of the specimens of N. randalli collected from the Lebanon coast indicate recent successful recruitment.

Summary The spawning periods, sex ratio and length at first maturity were determined for six commercially important finfish species collected from Oman's industrial trawl fishery. During 1 year of monthly sampling, biological data were collected on 5586 fish. ancova was used to compare the length–weight relationships between sexes. There were sex-specific differences in length-weight relationships for three of the six species examined. One serranid, Epinephelus diacanthus, and a sparid, Pagellus affinis, had sex ratios significantly different from the expected ratio of 1 : 1. Size–frequency data revealed more male fish in the larger size classes for E. diacanthus, P. affinis and Nemipterus japonicus. Data on the development of female gonads and Gonado-somatic Index (GSI) revealed that the six species spawned at different times of the year. With the exception of female E. diacanthus, all fish had low GSI values during the onset of the summer monsoon period in June. The proportion of immature vs mature fish in the sample differed among species. For two species, Lethrinus nebulosus and Cheimerius nufar, more than 40% of the fish sampled (both sexes) were immature. Approximately 33% female and 41% male P. affinis were also immature. Management options such as temporal and area closures are discussed in light of the large number of immature fish caught by the fishery.

A specimen of the Japanese threadfin bream Nemipterus japonicus was recorded for the first time from the Mediterranean Sea. The presence of this Indo-Pacific fish in the Mediterranean is evidently due to migration from the Red Sea via the Suez Canal.

Threadfin bream (Nemipterus japonicus) spray-dried hydro-lysate was prepared using Alcalase at predetermined optimum hydrolysis conditions consisting of pH 8.5, 60°C, 2% enzyme, and 2 h hydrolysis. The hydrolysate was evaluated for its preferred concentration in rice porridge. Its acceptability was compared with sauces and enzyme-treated rice and vegetable hydrolysate. The preferred concentration in rice porridge was found to be 3% (w/v) and bitterness increased with concentration of hydrolysate. Threadfin bream hydrolysate was preferred as much as soy sauce; however, it received a lower score for taste compared to fish sauce. In terms of color, threadfin bream hydrolysate received significantly (p < 0.05) lower score than Flavorzyme and Alcalase treated rice hydrolysate and Vegetable hydrolysate. Low preference to the hydrolysate could probably be due to its greenish yellow color in which the common color for flavorants are brownish. For overall acceptability, no significant difference was obser...

Catch rates and sizes of Saurida undosquamis and Nemipterus japonicus were more variable during daytime. But, Pomadasys stridens were more variable in night tows. Catch rates of S. undosquamis and N. japonicus were higher during daytime and of significantly small mean fish size. Inversely, P. stridens had significant higher catch rates and the fish caught were smaller in night tows. Variability of catch rate and size of Penaeus semisulcatus and Penaeus japonicus was higher at night. Daytime catch rates were significantly lower and of smaller mean shrimp size. Penaeus latisulcatus was available only at night.

Based on Nei's genetic distance Nemipterus peronii and Nemipterus japonicus showed a greater affinity to Parascolopsis aspinosa than to the other Nemipterus species studied. Significant differences between the Indian west and east coast samples of N. japonicus were detected. Polymorphic (P

A dorsal accessory duct (DAD) structure was found on the dorsal side of a functional testis in all males of both Nemipterus virgatus and N. japonicus, and determined to be homologous to the ovary, based on structural, histological and cytological evidence. Secretions, in the DAD varied according to reproductive season and were particularly marked in N. virgatus. The sexual pattern of both species was functional gonochorism. The secretions of the DAD suggest some unknown function, possibly associated with spawning, which has evidently arisen de novo within a rudimentary ovary: this is the only example of its type reported among hermaphroditic fishes.

Summary Weight–length relationships were estimated for 20 Lessepsian fish species of the Iskenderun Bay, namely, Apogon nigripinnis, Apogon queketti, Callionymus flamentosus, Cynoglossus sinusarabici, Dussumieria elopsoides, Etrumeus teres, Fistularia commersonii, Lagocephalus spadiceus, Lagocephalus suezensis, Leiognathus kluzingeri, Nemipterus japonicus, Oxyurichthys petersenii, Saurida undosquamis, Sillago sihama, Siganus luridus, Siganus rivulatus, Sphyraena chroysotaeina, Stephanolepis diaspros, Upeneus moluccensis and Upeneus pori. All regressions were found to be significant for all species. This study presents the first estimation of weight–length relationships for six species (A. queketti, E. teres,F. commersonii, L. suezensis, Nemipterus randalli, and S. luridus) inhabiting coastal areas of the northeastern Mediterranean Sea.

Dry salted mackerel and pink perch, prepared using a curing mixture, were stored at two temperatures ambient (26.8°C ± 3.3°C) and at 2.5°C ± 1°C. Changes in moisture, salt content, water activity (aw), peroxide value (PV), free fatty acids (FFA), thiobarbituric acid (TBA) number, total volatile base nitrogen (TVBN), halophilic bacterial count (HC) and sensory scores for overall acceptability were studied. Loss of moisture and absorption of salt were considerably higher in the products stored at ambient temperature. Salted mackerel had lower salt and lower moisture content than the pink perch. The decrease in aw was more pronounced at ambient temperature than at 2.5°C. TBA, FFA, PV, TVBN and HC increased during storage, thereby resulting in decreased sensory scores for overall acceptability. Further, the study indicates that salted pink perch was found to have 1.6 times longer shelf-life than the fatty mackerel at both ambient as well as cooler temperature.

The body burdens of environmentally persistent chlorinated insecticides such as DDT and its metabolites and HCH (BHC) isomers in 14 species of marine fishes collected from Tamil Nadu (Madras, Cuddalore, Nagapattinam and Tuticorin) and Pondicherry coasts of Bay of Bengal in South India were reported. The total HCH was more dominant than total DDT. Moreover α-HCH and p , p ′-DDT were greater among HCHs and DDTs respectively. Higher concentrations of both the compounds were detected in the tissues of rays ( Dasyatis sp.), Lates calcarifer, Scomberomorus guttatus and Nemipterus japonicus .

This communication gives data on the content of 10 trace metals in six commercially important fish species from the Arabian Sea.

The rate of fish invasion into the Mediterranean Sea has increased in recent decades; col- lectively they have significant ecological and economic impacts in the eastern Mediterranean. One of this species is Japanese thread fin bream Nemipterus japonicus that gradually get abundance in the Egyptian Mediterranean coast bottom trawl catch. During 2011, monthly samples of N. japonicus caught off Abu Qir area, Alexandria-Egypt, were used to estimate some biological parameters of this immigrant species. Females were more in abundance than male in the catch deviated to smaller size than male. Spawning season extended from April to November with highest activities in July. Maximum observed age was 3 years for both sexes with no significant difference between their lengths at age. Length-at-age was fitted to the Von Bertalanffy growth models. Present results of the Mediter- ranean Sea population showed lower biological (e.g. growth and longevity) condition comparing to original indo-pacific population. a 2014 Production and hosting by Elsevier B.V. on behalf of National Institute of Oceanography and Fisheries.