1. A Fluorescence Spot Test for Salicylate Determination.
- Author
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Linares, E.M., Palermo, L.T., Moreira, A.B., Sotomayor, M.D. P. T, and Kubota, L.T.
- Subjects
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FLUORESCENCE spectroscopy , *ASPIRIN , *SALICYLIC acid , *OPTICAL fibers , *FLUORIMETRY , *DRUG overdose - Abstract
A procedure based on a spot test for salicylate is presented. The method is simple, rugged and is based on the salycilate native fluorescence measurement on the surface of a filter paper by using optical fibers for light conduction. The fluorescence was linear at concentration range of 0.01–0.4 mmol l-1. The relative standard deviation of 5% (n=10) was observed. Detection and quantification limits were 4 and 14 µmol l-1, respectively. The results obtained in pharmaceutical salicylate determinations showed that practically no statistical difference existed with the pharmacopeical method (95% confidence level). The recovery studies in biological samples spiked with salicylic acid at overdose concentration levels showed that the matrix sample did not interfere in the method. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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