1. Yeast life-span extension by calorie restriction is independent of NAD fluctuation.
- Author
-
Anderson RM, Latorre-Esteves M, Neves AR, Lavu S, Medvedik O, Taylor C, Howitz KT, Santos H, and Sinclair DA
- Subjects
- Acetaldehyde metabolism, Aerobiosis, CCAAT-Binding Factor genetics, CCAAT-Binding Factor metabolism, Caloric Restriction, Culture Media, DNA-Binding Proteins, Genes, Fungal, Genes, Reporter, Histidine metabolism, Hydro-Lyases genetics, Hydro-Lyases metabolism, Longevity, Magnetic Resonance Spectroscopy, Recombinant Fusion Proteins metabolism, Repressor Proteins genetics, Repressor Proteins metabolism, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae growth & development, Saccharomyces cerevisiae Proteins genetics, Saccharomyces cerevisiae Proteins metabolism, Sirtuin 1, Sirtuin 2, Transcription Factors genetics, Transcription Factors metabolism, Bacterial Proteins, Histone Deacetylases metabolism, NAD metabolism, Saccharomyces cerevisiae physiology, Silent Information Regulator Proteins, Saccharomyces cerevisiae metabolism, Sirtuins metabolism
- Abstract
Calorie restriction (CR) slows aging in numerous species. In the yeast Saccharomyces cerevisiae, this effect requires Sir2, a conserved NAD+-dependent deacetylase. We report that CR reduces nuclear NAD+ levels in vivo. Moreover, the activity of Sir2 and its human homologue SIRT1 are not affected by physiological alterations in the NAD+:NADH ratio. These data implicate alternate mechanisms of Sir2 regulation by CR.
- Published
- 2003
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