8 results on '"Srijon Kaushik Banerjee"'
Search Results
2. The sensor kinase MtrB of Mycobacterium tuberculosis regulates hypoxic survival and establishment of infection
- Author
-
Manish Kumar, Sanjaya Kumar Sahu, Joyoti Basu, Srijon Kaushik Banerjee, Ramandeep Singh, Pushpa Gupta, Sudipto Saha, Arun Kumar Sharma, Shreya Bagchi, Manikuntala Kundu, Kuladip Jana, Debasree Sarkar, Suruchi Lata, and Umesh D. Gupta
- Subjects
Lung Diseases ,0301 basic medicine ,Regulator ,Virulence ,Microbiology ,Biochemistry ,Mycobacterium tuberculosis ,Mice ,03 medical and health sciences ,Bacterial Proteins ,Gene expression ,Animals ,Humans ,Gene Regulatory Networks ,Phosphorylation ,Promoter Regions, Genetic ,Molecular Biology ,Gene ,Antigens, Bacterial ,Mice, Inbred BALB C ,030102 biochemistry & molecular biology ,biology ,Kinase ,Macrophages ,Autophagosomes ,RNA-Binding Proteins ,Cell Biology ,biology.organism_classification ,Cell biology ,DNA-Binding Proteins ,Response regulator ,030104 developmental biology ,Regulon ,Biofilms ,Host-Pathogen Interactions ,Cytokines ,Lysosomes ,Protein Binding ,Transcription Factors - Abstract
Paired two-component systems (TCSs), having a sensor kinase (SK) and a cognate response regulator (RR), enable the human pathogen Mycobacterium tuberculosis to respond to the external environment and to persist within its host. Here, we inactivated the SK gene of the TCS MtrAB, mtrB, generating the strain ΔmtrB. We show that mtrB loss reduces the bacterium's ability to survive in macrophages and increases its association with autophagosomes and autolysosomes. Notably, the ΔmtrB strain was markedly defective in establishing lung infection in mice, with no detectable lung pathology following aerosol challenge. ΔmtrB was less able to withstand hypoxic and acid stresses and to form biofilms and had decreased viability under hypoxia. Transcriptional profiling of ΔmtrB by gene microarray analysis, validated by quantitative RT-PCR, indicated down-regulation of the hypoxia-associated dosR regulon, as well as genes associated with other pathways linked to adaptation of M. tuberculosis to the host environment. Using in vitro biochemical assays, we demonstrate that MtrB interacts with DosR (a noncognate RR) in a phosphorylation-independent manner. Electrophoretic mobility shift assays revealed that MtrB enhances the binding of DosR to the hspX promoter, suggesting an unexpected role of MtrB in DosR-regulated gene expression in M. tuberculosis. Taken together, these findings indicate that MtrB functions as a regulator of DosR-dependent gene expression and in the adaptation of M. tuberculosis to hypoxia and the host environment. We propose that MtrB may be exploited as a chemotherapeutic target against tuberculosis.
- Published
- 2019
3. The Intersection of the Staphylococcus aureus Rex and SrrAB Regulons: an Example of Metabolic Evolution That Maximizes Resistance to Immune Radicals
- Author
-
Amelia C. Stephens, Xingru Chen, Aidan Dmitriev, Elyse Paluscio, Anthony R. Richardson, Nicholas P. Vitko, and Srijon Kaushik Banerjee
- Subjects
coagulase-negative staphylococci ,Staphylococcus aureus ,Operon ,viruses ,Virulence ,Nitric Oxide ,medicine.disease_cause ,Regulon ,Microbiology ,Evolution, Molecular ,Immune system ,Bacterial Proteins ,Virology ,metabolic evolution ,medicine ,Humans ,Anaerobiosis ,redox signaling ,Staphylococcaceae ,Gene ,fermentation ,biology ,Staphylococcus simiae ,Gene Expression Regulation, Bacterial ,Staphylococcal Infections ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,immune radicals ,QR1-502 ,Repressor Proteins ,metabolism ,Research Article - Abstract
Staphylococcus aureus is the most pathogenic member of the Staphylococcaceae. While it acquired an arsenal of canonical virulence determinants that mediate pathogenicity, it has also metabolically adapted to thrive at sites of inflammation. Notably, it has evolved to grow in the presence of nitric oxide (NO·). To this end, we note that the Rex regulon, composed of genes encoding dehydrogenases, metabolite transporters, and regulators, is much larger in S. aureus than other Staphylococcus species. Here, we demonstrate that this expanded Rex regulon is necessary and sufficient for NO· resistance. Preventing its expression results in NO· sensitivity, and the closely related species, Staphylococcus simiae, also possesses an expanded Rex regulon and exhibits NO· resistance. We hypothesize that the expanded Rex regulon initially evolved to provide efficient anaerobic metabolism but that S. aureus has co-opted this feature to thrive at sites of inflammation where respiration is limited. One distinguishing feature of the Rex regulon in S. aureus is that it contains the srrAB two-component system. Here, we show that Rex blocks the ability of SrrA to auto-induce the operon, thereby preventing maximal SrrAB expression. This results in NO·-responsive srrAB expression in S. aureus but not in other staphylococci. Consequently, higher expression of cytochromes and NO· detoxification are also observed in S. aureus alone, allowing for continued respiration at NO· concentrations beyond that of S. simiae. We therefore contend that the intersection of the Rex and SrrAB regulons represents an evolutionary event that allowed S. aureus to metabolically adapt to host inflammatory radicals during infection. IMPORTANCE Pathogens must evolve virulence potential to improve transmission to new hosts as well as evolve metabolically to thrive within their current host. Staphylococcus aureus has achieved both of these, and here, we show that one such metabolic adaptation was the expansion of the Rex regulon. First, it affords S. aureus with efficient respiration-independent growth critical to surviving the inflammatory environment replete with respiration-inhibiting immune radicals. Second, it includes the srrAB operon encoding a two-component system critical to maximizing respiratory capacity in the face of host nitric oxide (NO·), a potent respiratory inhibitor. This second facet is only apparent in S. aureus and not in other closely related species. Thus, evolutionarily, it must have occurred relatively recently. The intertwining of the Rex and SrrAB regulons represents an important evolutionary event that affords S. aureus the metabolic flexibility required to thrive within inflamed tissue and cause disease.
- Published
- 2021
4. The Yersinia pestis GTPase BipA Promotes Pathogenesis of Primary Pneumonic Plague
- Author
-
William E. Goldman, Kara R. Eichelberger, Samantha D Crane, Richard C. Kurten, Srijon Kaushik Banerjee, and Roger D. Pechous
- Subjects
Pneumonic plague ,Virulence Factors ,Yersinia pestis ,Immunology ,Virulence ,Yersinia ,medicine.disease_cause ,Microbiology ,Bubonic plague ,Virulence factor ,GTP Phosphohydrolases ,Mice ,Bacterial Proteins ,medicine ,Animals ,Humans ,Pathogen ,Plague ,biology ,Pseudomonas aeruginosa ,pathogenesis ,medicine.disease ,biology.organism_classification ,Molecular Pathogenesis ,Mice, Inbred C57BL ,Disease Models, Animal ,Infectious Diseases ,Models, Animal ,Female ,Parasitology ,pneumonic plague ,bacterial GTPases - Abstract
Yersinia pestis is a highly virulent pathogen and the causative agent of bubonic, septicemic, and pneumonic plague. Primary pneumonic plague caused by inhalation of respiratory droplets contaminated with Y. pestis is nearly 100% lethal within 4 to 7 days without antibiotic intervention., Yersinia pestis is a highly virulent pathogen and the causative agent of bubonic, septicemic, and pneumonic plague. Primary pneumonic plague caused by inhalation of respiratory droplets contaminated with Y. pestis is nearly 100% lethal within 4 to 7 days without antibiotic intervention. Pneumonic plague progresses in two phases, beginning with extensive bacterial replication in the lung with minimal host responsiveness, followed by the abrupt onset of a lethal proinflammatory response. The precise mechanisms by which Y. pestis is able to colonize the lung and survive two very distinct disease phases remain largely unknown. To date, a few bacterial virulence factors, including the Ysc type 3 secretion system, are known to contribute to the pathogenesis of primary pneumonic plague. The bacterial GTPase BipA has been shown to regulate expression of virulence factors in a number of Gram-negative bacteria, including Pseudomonas aeruginosa, Escherichia coli, and Salmonella enterica serovar Typhi. However, the role of BipA in Y. pestis has yet to be investigated. Here, we show that BipA is a Y. pestis virulence factor that promotes defense against early neutrophil-mediated bacterial killing in the lung. This work identifies a novel Y. pestis virulence factor and highlights the importance of early bacterial/neutrophil interactions in the lung during primary pneumonic plague.
- Published
- 2021
5. Global mapping of MtrA-binding sites links MtrA to regulation of its targets in Mycobacterium tuberculosis
- Author
-
Manikuntala Kundu, Amar Chandra Mahatha, Arun Kumar Sharma, Sudipto Saha, Joyoti Basu, Ayan Chatterjee, Manish Kumar, and Srijon Kaushik Banerjee
- Subjects
0301 basic medicine ,Chromatin Immunoprecipitation ,Transcription, Genetic ,030106 microbiology ,Mutant ,Biology ,Microbiology ,Mycobacterium tuberculosis ,03 medical and health sciences ,Bacterial Proteins ,Transcriptional regulation ,Consensus sequence ,Nucleotide Motifs ,Phosphorylation ,Promoter Regions, Genetic ,Gene ,Genetics ,Binding Sites ,Macrophages ,Computational Biology ,Gene Expression Regulation, Bacterial ,biology.organism_classification ,Recombinant Proteins ,ChIP-sequencing ,DNA-Binding Proteins ,Response regulator ,Mutation ,ATP-Binding Cassette Transporters ,Chromatin immunoprecipitation ,Genome, Bacterial ,Genome-Wide Association Study ,Transcription Factors - Abstract
Mycobacterium tuberculosis employs two-component systems (TCSs) for survival within its host. The TCS MtrAB is conserved among mycobacteria. The response regulator MtrA is essential in M. tuberculosis. The genome-wide chromatin immunoprecipitation (ChIP) sequencing performed in this study suggested that MtrA binds upstream of at least 45 genes of M. tuberculosis, including those involved in cell wall remodelling, stress responses, persistence and regulation of transcription. It binds to the promoter regions and regulates the peptidoglycan hydrolases rpfA and rpfC, which are required for resuscitation from dormancy. It also regulates the expression of whiB4, a critical regulator of the oxidative stress response, and relF, one-half of the toxin-antitoxin locus relFG. We have identified a new consensus 9 bp loose motif for MtrA binding. Mutational changes in the consensus sequence greatly reduced the binding of MtrA to its newly identified targets. Importantly, we observed that overexpression of a gain-of-function mutant, MtrAY102C, enhanced expression of the aforesaid genes in M. tuberculosis isolated from macrophages, whereas expression of each of these targets was lower in M. tuberculosis overexpressing a phosphorylation-defective mutant, MtrAD56N. This result suggests that phosphorylated MtrA (MtrA-P) is required for the expression of its targets in macrophages. Our data have uncovered new MtrA targets that suggest that MtrA is required for a transcriptional response that likely enables M. tuberculosis to persist within its host and emerge out of dormancy when the conditions are favourable.
- Published
- 2018
6. Essential protein SepF of mycobacteria interacts with FtsZ and MurG to regulate cell growth and division
- Author
-
Manikuntala Kundu, Shamba Gupta, Arun Kumar Sharma, Joyoti Basu, Ayan Chatterjee, and Srijon Kaushik Banerjee
- Subjects
Regulation of gene expression ,biology ,Cell division ,Cell growth ,Mycobacterium smegmatis ,Gene Expression Regulation, Bacterial ,Mycobacterium tuberculosis ,Plasma protein binding ,N-Acetylglucosaminyltransferases ,biology.organism_classification ,Microbiology ,Bacterial cell structure ,Cell biology ,Cytoskeletal Proteins ,Bacterial Proteins ,Biochemistry ,biology.protein ,FtsZ ,Cell Division ,Cytokinesis ,Bacterial Outer Membrane Proteins ,Protein Binding - Abstract
Coordinated bacterial cell septation and cell wall biosynthesis require formation of protein complexes at the sites of division and elongation, in a temporally controlled manner. The protein players in these complexes remain incompletely understood in mycobacteria. Using in vitro and in vivo assays, we showed that Rv2147c (or SepF) of Mycobacterium tuberculosis interacts with the principal driver of cytokinesis, FtsZ. SepF also interacts with itself both in vitro and in vivo. Amino acid residues 189A, 190K and 215F are required for FtsZ-SepF interaction, and are conserved across Gram-positive bacteria. Using Mycobacterium smegmatis as a surrogate system, we confirmed that sepFMSMEG is essential. Knockdown of SepF led to cell elongation, defective growth and failure of FtsZ to localize to the site of division, suggesting that SepF assists FtsZ localization at the site of division. Furthermore, SepF interacted with MurG, a peptidoglycan-synthesizing enzyme, both in vitro and in vivo, suggesting that SepF could serve as a link between cell division and peptidoglycan synthesis. SepF emerges as a newly identified essential component of the cell division complex in mycobacteria.
- Published
- 2015
7. Targeting multiple response regulators of Mycobacterium tuberculosis augments the host immune response to infection
- Author
-
Reshma Alokam, Ranjeet Kumar, Arun Kumar Sharma, Perumal Yogeeswari, Joyoti Basu, Ayan Chatterjee, Manikuntala Kundu, Sanjaya Kumar Sahu, Dharmarajan Sriram, Srijon Kaushik Banerjee, Manish Kumar, Kuladip Jana, and Ramandeep Singh
- Subjects
Models, Molecular ,0301 basic medicine ,030106 microbiology ,Nitric Oxide ,Genome ,Article ,Mycobacterium tuberculosis ,Transcriptome ,Mice ,03 medical and health sciences ,Immune system ,Bacterial Proteins ,Autophagy ,Animals ,Humans ,Tuberculosis ,Cells, Cultured ,Genetics ,Binding Sites ,Multidisciplinary ,biology ,Gene Expression Profiling ,Macrophages ,DNA ,biology.organism_classification ,Cell biology ,Molecular Docking Simulation ,Gene expression profiling ,Response regulator ,RAW 264.7 Cells ,Regulon ,Mutation ,ATP-Binding Cassette Transporters ,Protein Binding - Abstract
The genome of M. tuberculosis (Mtb) encodes eleven paired two component systems (TCSs) consisting of a sensor kinase (SK) and a response regulator (RR). The SKs sense environmental signals triggering RR-dependent gene expression pathways that enable the bacterium to adapt in the host milieu. We demonstrate that a conserved motif present in the C-terminal domain regulates the DNA binding functions of the OmpR family of Mtb RRs. Molecular docking studies against this motif helped to identify two molecules with a thiazolidine scaffold capable of targeting multiple RRs and modulating their regulons to attenuate bacterial replication in macrophages. The changes in the bacterial transcriptome extended to an altered immune response with increased autophagy and NO production, leading to compromised survival of Mtb in macrophages. Our findings underscore the promise of targeting multiple RRs as a novel yet unexplored approach for development of new anti-mycobacterial agents particularly against drug-resistant Mtb.
- Published
- 2016
8. Polyphosphate kinase 1, a central node in the stress response network of Mycobacterium tuberculosis, connects the two-component systems MprAB and SenX3-RegX3 and the extracytoplasmic function sigma factor, sigma E
- Author
-
Jayanta Mukhopadhyay, Sourav Sanyal, Srijon Kaushik Banerjee, Manikuntala Kundu, and Rajdeep Banerjee
- Subjects
DNA, Bacterial ,Transcription, Genetic ,Electrophoretic Mobility Shift Assay ,Sigma Factor ,Biology ,Microbiology ,Phosphates ,Polyphosphate kinase ,Bacterial Proteins ,Transcription (biology) ,Sigma factor ,Stress, Physiological ,Electrophoretic mobility shift assay ,Gene Regulatory Networks ,Binding site ,Promoter Regions, Genetic ,Phosphotransferases (Phosphate Group Acceptor) ,fungi ,Phosphotransferases ,Gene Expression Regulation, Bacterial ,Mycobacterium tuberculosis ,Molecular biology ,Response regulator ,Phosphorylation ,Signal transduction ,Protein Kinases ,Protein Binding ,Signal Transduction - Abstract
Polyphosphate (poly P) metabolism regulates the stress response in mycobacteria. Here we describe the regulatory architecture of a signal transduction system involving the two-component system (TCS) SenX3–RegX3, the extracytoplasmic function sigma factor sigma E (SigE) and the poly P-synthesizing enzyme polyphosphate kinase 1 (PPK1). The ppk1 promoter of Mycobacterium tuberculosis is activated under phosphate starvation. This is attenuated upon deletion of an imperfect palindrome likely representing a binding site for the response regulator RegX3, a component of the two-component system SenX3–RegX3 that responds to phosphate starvation. Binding of phosphorylated RegX3 to this site was confirmed by electrophoretic mobility shift assay. The activity of the ppk1 promoter was abrogated upon deletion of a putative SigE binding site. Pull-down of SigE from M. tuberculosis lysates of phosphate-starved cells with a biotinylated DNA harbouring the SigE binding site confirmed the likely binding of SigE to the ppk1 promoter. In vitro transcription corroborated the involvement of SigE in ppk1 transcription. Finally, the overexpression of RseA (anti-SigE) attenuated ppk1 expression under phosphate starvation, supporting the role of SigE in ppk1 transcription. The regulatory elements identified in ppk1 transcription in this study, combined with our earlier observation that PPK1 is itself capable of regulating sigE expression via the MprAB TCS, suggest the presence of multiple positive-feedback loops in this signalling circuit. In combination with the sequestering effect of RseA, we hypothesize that this architecture could be linked to bistability in the system that, in turn, could be a key element of persistence in M. tuberculosis.
- Published
- 2013
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.