1. Engineering a bzd cassette for the anaerobic bioconversion of aromatic compounds.
- Author
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Zamarro MT, Barragán MJL, Carmona M, García JL, and Díaz E
- Subjects
- Acyl Coenzyme A metabolism, Anaerobiosis, Bacterial Proteins metabolism, Benzoates chemistry, Biodegradation, Environmental, Hydroxybutyrates chemistry, Hydroxybutyrates metabolism, Metabolic Engineering, Toluene metabolism, Acyl Coenzyme A genetics, Azoarcus genetics, Azoarcus metabolism, Bacterial Proteins genetics, Benzoates metabolism
- Abstract
Microorganisms able to degrade aromatic contaminants constitute potential valuable biocatalysts to deal with a significant reusable carbon fraction suitable for eco-efficient valorization processes. Metabolic engineering of anaerobic pathways for degradation and recycling of aromatic compounds is an almost unexplored field. In this work, we present the construction of a functional bzd cassette encoding the benzoyl-CoA central pathway for the anaerobic degradation of benzoate. The bzd cassette has been used to expand the ability of some denitrifying bacteria to use benzoate as sole carbon source under anaerobic conditions, and it paves the way for future pathway engineering of efficient anaerobic biodegraders of aromatic compounds whose degradation generates benzoyl-CoA as central intermediate. Moreover, a recombinant Azoarcus sp. CIB strain harbouring the bzd cassette was shown to behave as a valuable biocatalyst for anaerobic toluene valorization towards the synthesis of poly-3-hydroxybutyrate (PHB), a biodegradable and biocompatible polyester of increasing biotechnological interest as a sustainable alternative to classical oil-derived polymers., (© 2017 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.)
- Published
- 2017
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