1. Berberine attenuates sodium palmitate-induced lipid accumulation, oxidative stress and apoptosis in grass carp(Ctenopharyngodon idella)hepatocyte in vitro.
- Author
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Yang SS, Yu CB, Luo Z, Luo WL, Zhang J, Xu JX, and Xu WN
- Subjects
- Animals, Caspases metabolism, Cell Survival drug effects, Cells, Cultured, Cytochromes c metabolism, Mitochondria drug effects, Mitochondria metabolism, Triglycerides metabolism, Apoptosis drug effects, Berberine pharmacology, Carps, Hepatocytes drug effects, Lipid Metabolism drug effects, Oxidative Stress drug effects, Palmitic Acid pharmacology
- Abstract
The objective of this work was to investigate the effect of berberine (BBR) on the Cell viability, lipid accumulation, apoptosis, cytochrome c, caspase-9 and caspase-3 in lipid accumulation-hepatocytes induced by sodium palmitate in vitro. The lipid accumulation-hepatocytes (induced by 0.5 mM sodium palmitate for 24 h) were treated with 5 μM berberine for 12 h. Then, the Cell viability, intracellular triglyceride (TG) content, lipid peroxide (LPO), malonaldehyde (MDA) content, cytochrome c, caspase-9, caspase-3 and apoptosis were detected. Sodium palmitate decreased Cell viability and increased intracellular TG content, lipid droplet accumulation, LPO and MDA concentrations, caused caspase-3 and caspase-9 activation, then led to apoptosis accompanied by cytochrome c release from mitochondria into the cytoplasm. Beberine could improve intracellular lipid droplet accumulation and oxidative stress, while reduce apoptosis induced by sodium palmitate., (Copyright © 2019. Published by Elsevier Ltd.)
- Published
- 2019
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