1. The Effect of the Thermosensitive Biodegradable PLGA–PEG–PLGA Copolymer on the Rheological, Structural and Mechanical Properties of Thixotropic Self-Hardening Tricalcium Phosphate Cement
- Author
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Matej Dzurov, Marek Zboncak, Lucy Vojtová, Milan Krtička, Petr Polacek, Klara Castkova, Lenka Michlovská, Edgar B. Montufar, Veronika Pavlinakova, Kristyna Valova, Martin Trunec, Vera Lukasova, Michala Rampichová, Maria-Pau Ginebra, Tomas Suchy, Radek Sedlacek, Universitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica, Universitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits, Vysoké učení technické v Brně, České vysoké učení technické v Praze, Fakultní nemocnice Brno, and Akademie věd České republiky
- Subjects
Calcium Phosphates ,Biocompatibilitat ,Biocompatible Materials ,02 engineering and technology ,01 natural sciences ,Polyethylene Glycols ,Polymerization ,lcsh:Chemistry ,chemistry.chemical_compound ,Phase (matter) ,Materials Testing ,morphology ,Copolymer ,lcsh:QH301-705.5 ,Polyglactin 910 ,Spectroscopy ,Glycolic acid ,Cells, Cultured ,Shear thinning ,Molecular Structure ,Bone Cements ,General Medicine ,Dynamic mechanical analysis ,Hydrogen-Ion Concentration ,021001 nanoscience & nanotechnology ,Computer Science Applications ,rheology ,0210 nano-technology ,Thixotropy ,Materials science ,Cell Survival ,Polyesters ,Enginyeria biomèdica::Biomaterials [Àrees temàtiques de la UPC] ,macromolecular substances ,010402 general chemistry ,Catalysis ,Bone cements ,Article ,Inorganic Chemistry ,biocompatibility ,Humans ,Physical and Theoretical Chemistry ,Bone regeneration ,Molecular Biology ,Mechanical Phenomena ,Organic Chemistry ,technology, industry, and agriculture ,0104 chemical sciences ,thixotropic ,chemistry ,Chemical engineering ,lcsh:Biology (General) ,lcsh:QD1-999 ,injectable bone cements ,kinetics ,Ciments ossis ,Ethylene glycol - Abstract
The current limitations of calcium phosphate cements (CPCs) used in the field of bone regeneration consist of their brittleness, low injectability, disintegration in body fluids and low biodegradability. Moreover, no method is currently available to measure the setting time of CPCs in correlation with the evolution of the setting reaction. The study proposes that it is possible to improve and tune the properties of CPCs via the addition of a thermosensitive, biodegradable, thixotropic copolymer based on poly(lactic acid), poly(glycolic acid) and poly(ethylene glycol) (PLGA&ndash, PEG&ndash, PLGA) which undergoes gelation under physiological conditions. The setting times of alpha-tricalcium phosphate (&alpha, TCP) mixed with aqueous solutions of PLGA&ndash, PLGA determined by means of time-sweep curves revealed a lag phase during the dissolution of the &alpha, TCP particles. The magnitude of the storage modulus at lag phase depends on the liquid to powder ratio, the copolymer concentration and temperature. A sharp increase in the storage modulus was observed at the time of the precipitation of calcium deficient hydroxyapatite (CDHA) crystals, representing the loss of paste workability. The PLGA&ndash, PLGA copolymer demonstrates the desired pseudoplastic rheological behaviour with a small decrease in shear stress and the rapid recovery of the viscous state once the shear is removed, thus preventing CPC phase separation and providing good cohesion. Preliminary cytocompatibility tests performed on human mesenchymal stem cells proved the suitability of the novel copolymer/&alpha, TCP for the purposes of mini-invasive surgery.
- Published
- 2019
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