93 results on '"Cytoplasm -- Genetic aspects"'
Search Results
2. Flagellar formation in C-ring-defective mutants by overproduction of FliI, the ATPase specific for flagellar type III secretion
- Author
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Konishi, Manabu, Kanbe, Masaomi, McMurry, Jonathan L., and Aizawa, Shin-Ichi
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Flagella (Microbiology) -- Genetic aspects ,Flagella (Microbiology) -- Research ,Cytoplasm -- Genetic aspects ,Cytoplasm -- Research ,Adenosine triphosphatase -- Genetic aspects ,Adenosine triphosphatase -- Research ,Gene mutations -- Research ,Biological sciences - Abstract
The flagellar cytoplasmic ring (C ring), which consists of three proteins, FliG, FliM, and FliN, is located on the cytoplasmic side of the flagellum. The C ring is a multifunctional structure necessary for flagellar protein secretion, torque generation, and switching of the rotational direction of the motor. The deletion of any one of the fliG, fliM, and fliN genes results in a [Fla.sup.-] phenotype. Here, we show that the overproduction of the flagellum-specific ATPase FliI overcomes the inability of basal bodies with partial C-ring structures to produce complete flagella. Flagella made upon FliI overproduction were paralyzed, indicating that an intact C ring is essential for motor function. In FliN-or FliM-deficient mutants, flagellum production was about 10% of the wild-type level, while it was only a few percent in FliG-deficient mutants, suggesting that the size of partial C rings affects the extent of flagellation. For flagella made in C-ring mutants, the hook length varied considerably, with many being markedly shorter or longer than that of the wild type. The broad distribution of hook lengths suggests that defective C rings cannot control the hook length as tightly as the wild type even though FliK and FlhB are both intact. doi: 10.1128/JB.00601-09
- Published
- 2009
3. A viable Bacillus subtilis strain without functional extracytoplasmic function sigma genes
- Author
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Asai, Kei, Ishiwata, Keisuke, Matsuzaki, Kunihiko, and Sadaie, Yoshito
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Bacterial genetics -- Research ,Bacillus subtilis -- Genetic aspects ,Bacillus subtilis -- Physiological aspects ,Chromosome deletion -- Influence ,Gene mutations -- Influence ,Cytoplasm -- Genetic aspects ,Cytoplasm -- Properties ,Biological sciences - Abstract
We constructed a Bacillus subtilis Marburg strain that harbors deletion mutations in all seven extracytoplasmic function (ECF) sigma genes. The strain shows wild-type growth at 37[degrees]C both in a complex and in a synthetic medium and exhibits wild-type sporulation. ECF sigma genes of B. subtilis are dispensable as long as no stress is imposed, although they seem to be required for quick response to stresses.
- Published
- 2008
4. SUMO: ligases, isopeptidases and nuclear pores
- Author
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Melchior, Frauke, Schergaut, Marion, and Pichler, Andrea
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Biochemistry -- Research ,Cytoplasm -- Genetic aspects ,Cytoplasm -- Physiological aspects ,Enzymes -- Genetic aspects ,Enzymes -- Physiological aspects ,Ligases -- Genetic aspects ,Ligases -- Physiological aspects ,Proteins -- Genetic aspects ,Proteins -- Physiological aspects ,Ubiquitin -- Physiological aspects ,Yeast fungi -- Genetic aspects ,Yeast fungi -- Physiological aspects ,Biological sciences ,Chemistry - Abstract
Small ubiquitin-related modifier (SUMO) proteins are reversibly coupled to numerous intracellular targets and modulate their interactions, localization, activity or stability. Recent advances in the SUMO field have uncovered the first SUMO E3 ligases and point to a complex family of isopeptidases. SUMO has been linked to many different pathways, including nucleocytoplasmic transport. Modifying enzymes and an isopeptidase have been detected at nuclear pore complexes. In addition, studies in yeast suggest a requirement of SUMO conjugation for nuclear protein import, and specific SUMO targets depend on modification for nuclear import or export.
- Published
- 2003
5. APP processing is regulated by cytoplasmic phosphorylation
- Author
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Lee, Ming-Sum, Kao, Shih-Chu, Lemere, Cynthia A., Xia, Weiming, Tseng, Huang-Chun, Zhou, Ying, Neve, Rachael, Ahlijanian, Michael K., and Tsai, Li-Huei
- Subjects
Cytoplasm -- Genetic aspects ,Genetic regulation -- Physiological aspects ,Genetic markers -- Physiological aspects ,Secretion -- Genetic aspects ,Viral antibodies -- Genetic aspects ,Antibodies -- Genetic aspects ,Phosphorylation -- Analysis ,Peptides -- Genetic aspects ,Peptides -- Physiological aspects ,Amyloid beta-protein -- Physiological aspects ,Amyloid beta-protein -- Genetic aspects ,Gene mutations -- Physiological aspects ,Cytology -- Research ,Biological sciences - Abstract
Amyloid-[beta] peptide (A[beta]) aggregate in senile plaque is a key characteristic of Alzheimer's disease (AD). Here, we show that phosphorylation of amyloid precursor protein (APP) on threonine 668 (P-APP) may play a role in APP metabolism. In AD brains, P-APP accumulates in large vesicular structures in afflicted hippocampal pyramidal neurons that costain with antibodies against endosome markers and the [beta]secretase, BACE1. Western blot analysis reveals increased levels of T668-phosphorylated APP COOH-terminal fragments in hippocampal lysates from many AD but not control subjects. Importantly, P-APP cofractionates with endosome markers and BACE1 in an iodixanol gradient and displays extensive colocalization with BACE1 in rat primary cortical neurons. Furthermore, APP COOH-terminal fragments generated by BACE1 are preferentially phosphorylated on T668 verses those produced by [alpha]-secretase. The production of A[beta] is significantly reduced when phosphorylation of T668 is either abolished by mutation or inhibited by T668 kinase inhibitors. Together, these results suggest that T668 phosphorylation may facilitate the BACE1 cleavage of APP to increase A[beta] generation.
- Published
- 2003
6. Limitations of allotopic expression of mitochondrial genes in mammalian cells
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Oca-Cossio, Jose, Kenyon, Lesley, Hao, Huiling, and Moraes, Carlos T.
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Genetic research -- Analysis ,Polypeptides -- Genetic aspects ,Mitochondrial DNA -- Genetic aspects ,Cytoplasm -- Genetic aspects ,Gene expression -- Physiological aspects ,DNA -- Genetic aspects ,Biological sciences - Abstract
The possibility of expressing mitochondrial DNA-coded genes in the nuclear-cytoplasmic compartment provides an attractive system for genetic treatment of mitochondrial disorders associated with mitochondrial DNA mutations. In theory, by recoding mitochondrial genes to adapt them to the universal genetic code and by adding a DNA sequence coding for a mitochondrial-targeting sequence, one could achieve correct localization of the gene product. Such transfer has occurred in nature, and certain species of algae and plants express a number of polypeptides that are commonly coded by mtDNA in the nuclear-cytoplasmic compartment. In the present study, allotopic expression of three different mtDNA-coded polypeptides (ATPase8, apocytochrome b, and ND4) into COS-7 and HeLa cells was analyzed. Among these, only ATPase8 was correctly expressed and localized to mitochondria. The full-length, as well as truncated forms, of apocytochrome b and ND4 decorated the periphery of mitochondria, but also aggregated in fiber-like structures containing tubulin and in some cases also vimentin. The addition of a hydrophilic tail (EGFP) to the C terminus of these polypeptides did not change their localization. Overexpression of molecular chaperones also did not have a significant effect in preventing aggregations. Allotopic expression of apocytochrome b and ND4 induced a loss of mitochondrial membrane potential in transfected cells, which can lead to cell death. Our observations suggest that only a subset of mitochondrial genes can be replaced allotopically. Analyses of the hydrophobic patterns of different polypeptides suggest that hydrophobicity of the N-terminal segment is the main determinant for the importability of peptides into mammalian mitochondria.
- Published
- 2003
7. Uncovering multiple axonal targeting pathways in hippocampal neurons
- Author
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Wisco, Dolora, Anderson, Eric D., Chang, Michael C., Norden, Caren, Boiko, Tatiana, Folsch, Heike, and Winckler, Bettina
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Axons -- Genetic aspects ,Genetic regulation -- Physiological aspects ,Cytoplasm -- Genetic aspects ,Endocytosis -- Genetic aspects ,Endocytosis -- Analysis ,Cell membranes -- Genetic aspects ,Cell membranes -- Physiological aspects ,Cell adhesion -- Physiological aspects ,Cell adhesion -- Genetic aspects ,Neurons -- Genetic aspects ,Neurons -- Physiological aspects ,Membrane proteins -- Genetic aspects ,Membrane proteins -- Physiological aspects ,Cytology -- Research ,Biological sciences - Abstract
Neuronal polarity is, at least in part, mediated by the differential sorting of membrane proteins to distinct domains, such as axons and somata/dendrites. We investigated the pathways underlying the subcellular targeting of NgCAM, a cell adhesion molecule residing on the axonal plasma membrane. Following transport of NgCAM kinetically, surprisingly we observed a transient appearance of NgCAM on the somatodendritic plasma membrane. Down-regulation of endocytosis resulted in loss of axonal accumulation of NgCAM, indicating that the axonal localization of NgCAM was dependent on endocytosis. Our data suggest the existence of a dendrite-to-axon transcytotic pathway to achieve axonal accumulation. NgCAM mutants with a point mutation in a crucial cytoplasmic tail motif (YRSL) are unable to access the transcytotic route. Instead, they were found to travel to the axon on a direct route. Therefore, our results suggest that multiple distinct pathways operate in hippocampal neurons to achieve axonal accumulation of membrane proteins.
- Published
- 2003
8. NuSAP, a novel microtubule-associated protein involved in mitotic spindle organization
- Author
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Raemaekers, Tim, Ribbeck, Katharina, Beaudouin, Joel, Annaert, Wim, Van Camp, Mark, Stockmans, Ingrid, Smets, Nico, Bouillon, Roger, Ellenberg, Jan, and Carmeliet, Geert
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Chromosomes -- Genetic aspects ,Chromosomes -- Physiological aspects ,Cell proliferation -- Physiological aspects ,Cell proliferation -- Genetic aspects ,Cytoplasm -- Genetic aspects ,Cytoplasm -- Physiological aspects ,Microtubules -- Genetic aspects ,Microtubules -- Physiological aspects ,Cell division -- Physiological aspects ,Cell division -- Genetic aspects ,Gene expression -- Physiological aspects ,Proteins -- Physiological aspects ,Proteins -- Genetic aspects ,Cytology -- Research ,Biological sciences - Abstract
Here, we report on the identification of nucleolar spindle-associated protein (NuSAP), a novel 55-kD vertebrate protein with selective expression in proliferating cells. Its mRNA and protein levels peak at the transition of G2 to mitosis and abruptly decline after cell division. Microscopic analysis of both fixed and live mammalian cells showed that NuSAP is primarily nucleolar in interphase, and localizes prominently to central spindle microtubules during mitosis. Direct interaction of NuSAP with microtubules was demonstrated in vitro. Overexpression of NuSAP caused profound bundling of cytoplasmic microtubules in interphase cells, and this relied on a COOH-terminal microtubule-binding domain. In contrast, depletion of NuSAP by RNA interference resulted in aberrant mitotic spindles, defective chromosome segregation, and cytokinesis. In addition, many NuSAP-depleted interphase cells had deformed nuclei. Both overexpression and knockdown of NuSAP impaired cell proliferation. These results suggest a crucial role for NuSAP in spindle microtubule organization.
- Published
- 2003
9. Nup358 integrates nuclear envelope breakdown with kinetochore assembly
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Salina, Davide, Enarson, Paul, Rattner, J.B., and Burke, Brian
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Proteins -- Physiological aspects ,Proteins -- Genetic aspects ,Metazoa -- Physiological aspects ,Metazoa -- Genetic aspects ,Mitosis -- Genetic aspects ,Mitosis -- Physiological aspects ,Cytoplasm -- Physiological aspects ,Cytoplasm -- Genetic aspects ,Chromosomes -- Physiological aspects ,Chromosomes -- Genetic aspects ,Cell research -- Analysis ,Kinetochores ,Biological sciences - Abstract
Nuclear envelope breakdown (NEBD) and release of condensed chromosomes into the cytoplasm are key events in the early stages of mitosis in metazoans. NEBD involves the disassembly of all major structural elements of the nuclear envelope, including nuclear pore complexes (NPCs), and the dispersal of nuclear membrane components. The breakdown process is facilitated by microtubules of the mitotic spindle. After NEBD, engagement of spindle microtubules with chromosome-associated kinetochores leads to chromatid segregation. Several NPC subunits relocate to kinetochores after NEBD. siRNA-mediated depletion of one of these proteins, Nup358, reveals that it is essential for kinetochore function. In the absence of Nup358, chromosome congression and segregation are severely perturbed. At the same time, the assembly of other kinetochore components is strongly inhibited, leading to aberrant kinetochore structure. The implication is that Nup358 plays an essential role in integrating NEBD with kinetochore maturation and function. Mitotic arrest associated with Nup358 depletion further suggests that mitotic checkpoint complexes may remain active at nonkinetochore sites.
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- 2003
10. Centrosome positioning in interphase cells
- Author
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Burakov, Anton, Nadezhdina, Elena, Slepchenko, Boris, and Rodionov, Vladimir
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Dynein -- Physiological aspects ,Dynein -- Genetic aspects ,Microtubules -- Physiological aspects ,Microtubules -- Genetic aspects ,Cytoplasm -- Genetic aspects ,Cytoplasm -- Physiological aspects ,Centrosomes -- Physiological aspects ,Centrosomes -- Genetic aspects ,Cell research -- Analysis ,Biological sciences - Abstract
The position of the centrosome is actively maintained at the cell center, but the mechanisms of the centering force remain largely unknown. It is known that centrosome positioning requires a radial array of cytoplasmic microtubules (MTs) that can exert pushing or pulling forces involving MT dynamics and the activity of cortical MT motors. It has also been suggested that actomyosin can play a direct or indirect role in this process. To examine the centering mechanisms, we introduced an imbalance of forces acting on the centrosome by local application of an inhibitor of MT assembly (nocodazole), and studied the resulting centrosome displacement. Using this approach in combination with microinjection of function-blocking probes, we found that a MT-dependent dynein pulling force plays a key role in the positioning of the centrosome at the cell center, and that other forces applied to the centrosomal MTs, including actomyosin contractility, can contribute to this process.
- Published
- 2003
11. Overproduction of inactive variants of the murein synthase PBP1B causes lysis in Escherichia coli
- Author
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Meisel, Ute, Holtje, Joachim-Volker, and Vollmer, Waldemar
- Subjects
Cells -- Genetic aspects ,Cytoplasm -- Genetic aspects ,Peptidoglycans -- Genetic aspects ,Binding proteins -- Genetic aspects ,Escherichia coli -- Genetic aspects ,Penicillin -- Physiological aspects ,Bacteriology -- Research ,Biological sciences - Abstract
Penicillin-binding protein 1B (PBP1B) of Escherichia coil is a bifunctional murein synthase containing both a transpeptidase domain and a transglycosylase domain. The protein is present in three forms ([alpha], [beta], and [gamma]) which differ in the length of their N-terminal cytoplasmic region. Expression piilasmids allowing the production of native PBP1B or of PBP1B variants with an inactive transpeptidase or transglycosylase domain or both were constructed. The inactive domains contained a single amino acid exchange in an essential active-site residue. Overproduction of the inactive PBP1B variants, but not of the active proteins, caused lysis of wild-type cells. The cells became tolerant to lysis by inactive PBP1B at a pH of 5.0, which is similar to the known tolerance for penicillin-induced lysis under acid pH conditions. Lysis was also reduced in mutant strains lacking several murein hydrolases. In particular, a strain devoid of activity of all known lytic transglycosylases was virtually tolerant, indicating that mainly the lyric transglycosylases are responsible for the observed lysis effect. A possible structural interaction between PBP1B and murein hydrolases in vivo by the formation of a muitienzyme complex is discussed.
- Published
- 2003
12. Calcium gradient dependence of Neurospora crassa hyphal growth
- Author
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Silverman-Gavrila, Lorelei B. and Lew, Roger R.
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Neurospora -- Physiological aspects ,Neurospora -- Genetic aspects ,Calcium, Dietary -- Physiological aspects ,Cytoplasm -- Physiological aspects ,Cytoplasm -- Genetic aspects ,Microbiology -- Research ,Biological sciences - Abstract
A tip-high cytoplasmic calcium gradient has been identified as a requirement for hyphal growth in the fungus Neurospora crassa. The [Ca.sup.2+] gradient is less steep compared to wall vesicle, wall incorporation and vesicular [Ca.sup.2+] gradients, but this can be explained by [Ca.sup.2+] diffusion. Analysis of the relation between the rate of hyphal growth and the spatial distribution of tip-localized calcium indicates that hyphal growth rates depend upon the tip-localized calcium concentration. It is not the steepness of the calcium gradient, but tip-localized calcium and the difference in tip-localized calcium versus subapical calcium concentration which correlate closely with hyphal growth rate. A minimal concentration difference between the apex and subapical region of 30 nM is required for growth to occur. The calcium concentration dependence of growth may relate directly to biochemical functions of calcium in hyphal extension, such as vesicle fusion and enzyme activation during cellular expansion. Initiation of tip growth may rely upon random [Ca.sup.2+] motions causing localized regions of elevated calcium. Continued hyphal expansion may activate a stretch-activated phospholipase C which would increase tip-localized inositol 1,4,5-trisphosphate (I[P.sub.3]). Hyphal expansion, induced by mild hypoosmotic treatment, does increase diacylglycerol, the other product of phospholipase C activity. This is consistent with evidence that I[P.sub.3]-activated [Ca.sup.2+] channels generate and maintain the tip-high calcium gradient.
- Published
- 2003
13. Regulation of the Bacillus subtilis extracytoplasmic function protein [[sigma].sup.y] and its target promoters
- Author
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Cao, Min, Salzberg, Letal, Tsai, Ching Sung, Mascher, Thorsten, Bonilla,Carla, Wang,Tao, Ye, Rick W., Marquez-Magana, Leticia, and Helmann, John D.
- Subjects
Cytoplasm -- Genetic aspects ,Genetic transcription -- Physiological aspects ,Gene mutations -- Physiological aspects ,Genetic regulation -- Physiological aspects ,Gene expression -- Physiological aspects ,Operons -- Genetic aspects ,Bacillus subtilis -- Genetic aspects ,Bacteriology -- Research ,Biological sciences - Abstract
The Bacillus subtilis extracytoplasmic function sigma factor [[sigma].sup.Y] is of unknown function. We demonstrate that the sigY operon is expressed from an autoregulatory promoter site, [P.sub.Y]. We selected for transposon-induced mutations that upregulate [P.sub.Y] transcription in an attempt to identify genes involved in [sigma].sup.Y] regulation. The resulting insertions disrupted yxlC, the gene immediately downstream of sigY. However, the phenotype of the yxlC::Tn10 insertion was due to polarity on the downstream genes of the sigY operon; a nonpolar insertion in yxlC did not lead to derepression of [P.sub.y] Further analyses revealed that both yxlD and yxlE encoded proteins important for the negative regulation of [[sigma].sup.Y] activity. A comparison of the transcriptomes of wild-type and yxlC::Tn10 mutant strains revealed elevated expression of several operons. However, only one additional gene, ybgB, was unambiguously identified as a direct target for [[sigma].sup.y] This was supported by analysis of direct targets for [[sigma].sup.Y] transcription with whole-genome runoff transcription followed by macroarray analysis.
- Published
- 2003
14. Probing conservation of HAMP linker structure and signal transduction mechanism through analysis of hybrid sensor kinases
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Appleman, J. Alex, Chen, Li-Ling, and Stewart, Valley
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Nitrites -- Physiological aspects ,Nitrates -- Physiological aspects ,Cytoplasm -- Genetic aspects ,Escherichia coli -- Genetic aspects ,Chemotaxis -- Physiological aspects ,Chemotaxis -- Genetic aspects ,Ligands (Biochemistry) ,Bacteriology -- Research ,Biological sciences - Abstract
The HAMP linker, a predicted structural element observed in many sensor kinases and methyl-accepting chemotaxis proteins, transmits signals between sensory input modules and output modules. HAMP linkers are located immediately inside the cytoplasmic membrane and are predicted to form two short amphipathic [alpha]-helices (AS-1 and AS-2) joined by an unstructured connector. HAMP linkers are found in the Escherichia coli nitrate- and nitrite-responsive sensor kinases NarX and NarQ (which respond to ligand by increasing kinase activity) and the sensor kinase CpxA (which responds to ligand by decreasing kinase activity). We constructed a series of hybrids with fusion points throughout the HAMP linker, in which the sensory modules of NarX or NarQ are fused to the transmitter modules of NarX, NarQ, or CpxA. A hybrid of the NarX sensor module and the CpxA HAMP linker and transmitter module (NarX-CpxA. 1) responded to nitrate by decreasing kinase activity, whereas a hybrid in which the HAMP linker of NarX was replaced by that of CpxA (NarX-CpxA-NarX-1) responded to nitrate by increasing kinase activity. However, sequence variations between HAMP linkers do not allow free exchange of HAMP linkers or their components. Certain deletions in the NarX HAMP linker resulted in characteristic abnormal responses to ligand; similar deletions in the NarQ and NarX-CpxA-1 HAMP linkers resulted in responses to ligand generally similar to those seen in NarX. We conclude that the structure and action of the HAMP linker are conserved and that the HAMP linker transmits a signal to the output domain that ligand is bound.
- Published
- 2003
15. Role of Pseudomonas putida tol-oprL gene products in uptake of solutes through the cytoplasmic membrane
- Author
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Llamas, Maria A., Rodriguez-Herva, Jose J., Hancock, Robert E.W., Bitter, Wilbert, Tommassen, Jan, and Ramos, Juan L.
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Membrane proteins -- Genetic aspects ,Glycerol -- Physiological aspects ,Glycerin -- Physiological aspects ,Arginine -- Physiological aspects ,Fructose -- Physiological aspects ,Pseudomonas -- Genetic aspects ,Cytoplasm -- Genetic aspects ,Cell membranes -- Genetic aspects ,Gram-negative bacteria -- Genetic aspects ,Gram-negative bacteria -- Growth ,Bacterial proteins -- Genetic aspects ,Bacteriology -- Research ,Company growth ,Biological sciences - Abstract
Proteins of the Tol-Pal (Tol-OprL) system play a key role in the maintenance of outer membrane integrity and cell morphology in gram-negative bacteria. Here we describe an additional role for this system in the transport of various carbon sources across the cytoplasmic membrane. Growth of Pseudomonas putida tol-oprL mutant strains in minimal medium with glycerol, fructose, or arginine was impaired, and the growth rate with succinate, proline, or sucrose as the carbon source was lower than the growth rate of the parental strain. Assays with radiolabeled substrates revealed that the rates of uptake of these compounds by mutant cells were lower than the rates of uptake by the wild-type strain. The pattern and amount of outer membrane protein in the P. putida tol-oprL mutants were not changed, suggesting that the transport defect was not in the outer membrane. Consistently, the uptake of radiolabeled glucose and glycerol in spheroplasts was defective in the P. putida tol-oprL mutant strains, suggesting that there was a defect at the cytoplasmic membrane level. Generation of a proton motive force appeared to be unaffected in these mutants. To rule out the possibility that the uptake defect was due to a lack of specific transporter proteins, the PutP symporter was overproduced, but this overproduction did not enhance proline uptake in the tol-oprL mutants. These results suggest that the Tol-OprL system is necessary for appropriate functioning of certain uptake systems at the level of the cytoplasmic membrane.
- Published
- 2003
16. Interplay of the Czc system and two p-type ATPases in conferring metal resistance to Ralstonia metallidurans
- Author
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Legatzki, Antje, Grass, Gregor, Anton, Andreas, Rensing, Christopher, and Nies, Dietrich H.
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Cytoplasm -- Genetic aspects ,Adenosine triphosphatase -- Physiological aspects ,Gene expression -- Physiological aspects ,Escherichia coli -- Genetic aspects ,Cells -- Genetic aspects ,Zinc -- Physiological aspects ,Cadmium -- Physiological aspects ,Bacteriology -- Research ,Biological sciences - Abstract
Cadmium and zinc are removed from cells of Ralstonia metallidurans by the CzcCBA efflux pump and by two soft-metal-transporting P-type ATPases, CadA and ZntA. The czcCBA genes are located on plasmid pMOL30, and the cadA and zntA genes are on the bacterial chromosome. Expression of zntA from R. metallidurans in Escherichia coli predominantly mediated resistance to zinc, and expression of cadA predominantly mediated resistance to cadmium. Both transporters decreased the cellular content of zinc or cadmium in this host. In the plasmid-free R. metallidurans strain AE104, single gene deletions of cadA or zntA had only a moderate effect on cadmium and zinc resistance, but zinc resistance decreased 6-fold and cadmium resistance decreased 350-fold in double deletion strains. Neither single nor double gene deletions affected zinc resistance in the presence of czcCBA. In contrast, cadmium resistance of the cadA zntA double mutant could be elevated only partially by the presence of CzcCBA. lacZ reporter gene fusions indicated that expression of cadA was induced by cadmium but not by zinc in R. metallidurans strain AE104. In the absence of the zntA gene, expression of cadA occurred at lower cadmium concentrations and zinc now served as an inducer. In contrast, expression of zntA was induced by both zinc and cadmium, and the induction pattern did not change in the presence or absence of CadA. However, expression of both genes, zntA and cadA, was diminished in the presence of CzcCBA. This indicated that CzcCBA efficiently decreased cytoplasmic cadmium and zinc concentrations. It is discussed whether these data favor a model in which the cations are removed either from the cytoplasm or the periplasm by CzcCBA.
- Published
- 2003
17. The cytoplasmic domain of the plasmodium falciparum ligand EBA-175 is essential for invasion but not protein trafficking
- Author
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Gilberger, Tim-Wolf, Thompson, Jennifer K., Reed, Michael B., Good, Robert T., and Cowman, Alan F.
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Gene expression -- Physiological aspects ,Erythrocytes -- Physiological aspects ,Erythrocytes -- Genetic aspects ,Plasmodium falciparum -- Physiological aspects ,Plasmodium falciparum -- Genetic aspects ,Cytoplasm -- Physiological aspects ,Cytoplasm -- Genetic aspects ,Binding proteins -- Physiological aspects ,Binding proteins -- Genetic aspects ,Cytology -- Research ,Biological sciences - Abstract
The invasion of host cells by the malaria parasite Plasmodium falciparum requires specific protein--protein interactions between parasite and host receptors and an intracellular translocation machinery to power the process. The transmembrane erythrocyte binding protein-175 (EBA-175) and thrombospondin-related anonymous protein (TRAP) play central roles in this process. EBA-175 binds to glycophorin A on human erythrocytes during the invasion process, linking the parasite to the surface of the host cell. In this report, we show that the cytoplasmic domain of EBA-175 encodes crucial information for its role in merozoite invasion, and that trafficking of this protein is independent of this domain. Further, we show that the cytoplasmic domain of TRAP, a protein that is not expressed in merozoites but is essential for invasion of liver cells by the sporozoite stage, can substitute for the cytoplasmic domain of EBA-175. These results show that the parasite uses the same components of its cellular machinery for invasion regardless of the host cell type and invasive form.
- Published
- 2003
18. Critical roles for the COOH-terminal NITY and RGT sequences of the integrin [[beta].sub.3] cytoplasmic domain in inside-out and outside-in signaling
- Author
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Xi, Xiaodong, Bodnar, Richard J., Li, Zhenyu, Lam, Stephen C.-T., and Du, Xiaoping
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Calcium compounds -- Physiological aspects ,Gene mutations -- Physiological aspects ,Glycoproteins -- Genetic aspects ,Glycoproteins -- Physiological aspects ,Cell adhesion -- Physiological aspects ,Cell adhesion -- Genetic aspects ,Gene expression -- Physiological aspects ,Cytoplasm -- Physiological aspects ,Cytoplasm -- Genetic aspects ,Integrins -- Physiological aspects ,Integrins -- Genetic aspects ,Cytology -- Research ,Biological sciences - Abstract
Bidirectional signaling of integrin [[alpha].sub.[parallel]b][[beta].sub.3] requires the [[beta].sub.3] cytoplasmic domain. To determine the sequence in the [[beta].sup.3] cytoplasmic domain that is critical to integrin signaling, cell lines were established that coexpress the platelet receptor for von Willebrand factor (vWF), glycoprotein lb-IX, integrin [[alpha].sub.[parallel]b], and mutants of [[beta].sub.3] with truncations at sites COOH terminal to [T.sup.741], [Y.sup.747], [F.sup.754], and [Y.sup.759]. Truncation at [Y.sup.759] did not affect integrin activation, as indicated by vWF-induced fibrinogen binding, but affected cell spreading and stable adhesion. Thus, the COOH-terminal RGT sequence of [beta]3 is important for outside-in signaling but not inside-out signaling. In contrast, truncation at [F.sup.754], [Y.sup.747, or [T.sup.741] completely abolished integrin activation. A point mutation replacing [Y.sup.759 with alanine also abolished integrin activation. Thus, the [T.sup.755]NIT[Y.sup.759] sequence of [[beta].sub.3], containing an NXXY motif, is critical to inside-out signaling, whereas the intact COOH terminus is important for outside-in signaling. In addition, we found that the calcium-dependent protease calpain preferentially cleaves at [Y.sup.759] in a population of [[beta].sub.3] during platelet aggregation and adhesion, suggesting that calpain may selectively regulate integrin outside-in signaling.
- Published
- 2003
19. A novel role for dp115 in the organization of tER sites in Drosophila
- Author
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Kondylis, Vangelis and Rabouille, Catherine
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Proteins -- Physiological aspects ,Proteins -- Genetic aspects ,Cytoplasm -- Genetic aspects ,Cytoplasm -- Physiological aspects ,Immunofluorescence -- Usage ,Fluorescent antibody technique -- Usage ,Golgi apparatus -- Genetic aspects ,Golgi apparatus -- Physiological aspects ,RNA -- Genetic aspects ,Drosophila -- Physiological aspects ,Drosophila -- Genetic aspects ,Cytology -- Research ,Biological sciences - Abstract
Here, we describe that depletion of the Drosophila homologue of p115 (dp115) by RNA interference in Drosophila S2 cells led to important morphological changes in the Golgi stack morphology and the transitional ER (tER) organization. Using conventional and immunoelectron microscopy and confocal immunofluorescence microscopy, we show that Golgi stacks were converted into clusters of vesicles and tubules, and that the tERs (marked by Sec23p) lost their focused organization and were now dispersed throughout the cytoplasm. However, we found that this morphologically altered exocytic pathway was nevertheless largely competent in anterograde protein transport using two different assays. The effects were specific for dp115. Depletion of the Drosophila homologues of GM130 and syntaxin 5 (dSed5p) did not lead to an effect on the tER organization, though the Golgi stacks were greatly vesiculated in the cells depleted of dSed5p. Taken together, these studies suggest that dp115 could be implicated in the architecture of both the Golgi stacks and the tER sites.
- Published
- 2003
20. Identification of synaptotagmin effectors via acute inhibition of secretion from cracked PC12 cells
- Author
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Tucker, Ward C., Edwardson, J. Michael, Bai, Jihong, Kim, Hyun-Jung, Martin, Thomas F.J., and Chapman, Edwin R.
- Subjects
Cytoplasm -- Genetic aspects ,Cytoplasm -- Physiological aspects ,Gene expression -- Physiological aspects ,Calcium compounds -- Physiological aspects ,Secretion -- Physiological aspects ,Secretion -- Genetic aspects ,Neurons -- Physiological aspects ,Neurons -- Genetic aspects ,Cell membranes -- Physiological aspects ,Cell membranes -- Genetic aspects ,Genetic regulation -- Physiological aspects ,Cytology -- Research ,Biological sciences - Abstract
The synaptotagmins (syts) are a family of membrane proteins proposed to regulate membrane traffic in neuronal and nonneuronal cells. In neurons, the [Ca.sup.2+]-sensing ability of syt I is critical for fusion of docked synaptic vesicles with the plasma membrane in response to stimulation. Several putative [Ca.sup.2+]-syt effectors have been identified, but in most cases the functional significance of these interactions remains unknown. Here, we have used recombinant C2 domains derived from the cytoplasmic domains of syts I-XI to interfere with endogenous syt-effector interactions during [Ca.sup.2+]-triggered exocytosis from cracked PC12 cells. Inhibition was closely correlated with syntaxin-SNAP-25 and phosphatidylinositol 4,5-bisphosphate (PI[P.sub.2])--binding activity. Moreover, we measured the expression levels of endogenous syts in PC12 cells; the major isoforms are I and IX, with trace levels of VII. As expected, if syts I and IX function as [Ca.sup.2+] sensors, fragments from these isoforms blocked secretion. These data suggest that syts trigger fusion via their [Ca.sup.2+]-regulated interactions with t-SNAREs and PI[P.sub.2], target molecules known to play critical roles in exocytosis.
- Published
- 2003
21. rpoN, mmoR and mmoG, genes involved in regulating the expression of soluble methane monooxygenase in Methylosinus trichosporium OB3b
- Author
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Stafford, Graham P., Scanlan, Julie, McDonald, Ian R., and Murrell, J. Colin
- Subjects
Nitrogen -- Physiological aspects ,Genetic regulation -- Physiological aspects ,Gene expression -- Physiological aspects ,Operons -- Genetic aspects ,Operons -- Physiological aspects ,Copper -- Physiological aspects ,Genetic transcription -- Physiological aspects ,Cytoplasm -- Physiological aspects ,Cytoplasm -- Genetic aspects ,Methanol -- Physiological aspects ,Methanobacteriaceae -- Physiological aspects ,Methane -- Physiological aspects ,Microbiology -- Research ,Biological sciences - Abstract
The methanotrophic bacterium Methylosinus trichosporium OB3b converts methane to methanol using two distinct forms of methane monooxygenase (MMO) enzyme: a cytoplasmic soluble form (sMMO) and a membrane-bound form (pMMO). The transcription of these two operons is known to proceed in a reciprocal fashion with sMMO expressed at Iow copper-to-biomass ratios and pMMO at high copper-to-biomass ratios. Transcription of the smmo operon is initiated from a [[sigma].sup.N] promoter 5' of mmoX. In this study the genes encoding [[sigma].sup.N] (rpoN) and a typical [[sigma].sup.N] -dependent transcriptional activator (mmoR) were cloned and sequenced, mmoR, a regulatory gene, and mmoG, a gene encoding a GroEL homologue, lie 5' of the structural genes for the sMMO enzyme. Subsequent mutation of rpoN and mmoR by marker-exchange mutagenesis resulted in strains Gm1 and JS1, which were unable to express functional sMMO or initiate transcription of mmoX. An rpoN mutant was also unable to fix nitrogen or use nitrate as sole nitrogen source, indicating that [[sigma].sup.N] plays a role in both nitrogen and carbon metabolism in Ms. trichosporium OB3b. The data also indicate that mmoG is transcribed in a [[sigma].sup.N] and MmoR-independent manner. Marker-exchange mutagenesis of mmoG revealed that MmoG is necessary for smmo gene transcription and activity and may be an MmoR-specific chaperone required for functional assembly of transcriptionally competent MmoR in vivo. The data presented allow the proposal of a more complete model for copper-mediated regulation of smmo gene expression.
- Published
- 2003
22. PKA phosphorylation activates the calcium release channel (ryanodine receptor) in skeletal muscle: defective regulation in heart failure
- Author
-
Reiken, Steven, Lacampagne, Alain, Zhou, Hua, Kherani, Aftab, Lehnart, Stephan E., Ward, Chris, Huang, Fannie, Gaburjakova, Marta, Gaburjakova, Jana, Rosemblit, Nora, Warren, Michelle S., He, Kun-lun, Yi, Geng-hua, Wang, Jie, Burkhoff, Daniel, Vassort, Guy, and Marks, Andrew R.
- Subjects
Cytology -- Research ,Calcium compounds -- Physiological aspects ,Sarcoplasmic reticulum -- Physiological aspects ,Sarcoplasmic reticulum -- Genetic aspects ,Cytoplasm -- Physiological aspects ,Cytoplasm -- Genetic aspects ,Carrier proteins -- Genetic aspects ,Carrier proteins -- Physiological aspects ,Nervous system, Sympathetic -- Physiological aspects ,Phosphorylation -- Physiological aspects ,Muscles -- Physiological aspects ,Muscles -- Genetic aspects ,Biological sciences - Abstract
The type 1 ryanodine receptor (RyR1) on the sarcoplasmic reticulum (SR) is the major calcium ([Ca.sup.2+]) release channel required for skeletal muscle excitation--contraction (EC) coupling. RyR1 function is modulated by proteins that bind to its large cytoplasmic scaffold domain, including the FK506 binding protein (FKBP12) and PKA. PKA is activated during sympathetic nervous system (SNS) stimulation. We show that PKA phosphorylation of RyR1 at [Ser.sup.2843] activates the channel by releasing FKBP12. When FKB12 is bound to RyR1, it inhibits the channel by stabilizing its closed state. RyR1 in skeletal muscle from animals with heart failure (HF), a chronic hyperadrenergic state, were PKA hyperphosphorylated, depleted of FKBP12, and exhibited increased activity, suggesting that the channels are 'leaky.' RyR1 PKA hyperphosphorylation correlated with impaired SR [Ca.sup.2+] release and early fatigue in HF skeletal muscle. These findings identify a novel mechanism that regulates RyR1 function via PKA phosphorylation in response to SNS stimulation. PKA hyperphosphorylation of RyR1 may contribute to impaired skeletal muscle function in HF, suggesting that a generalized EC coupling myopathy may play a role in HF.
- Published
- 2003
23. Formation of filopodia-like bundles in vitro from a dendritic network
- Author
-
Vignjevic, Danijela, Yarar, Defne, Welch, Matthew D., Peloquin, John, Svitkina, Tatyana, and Borisy, Gary G.
- Subjects
Cytology -- Research ,Actin -- Physiological aspects ,Actin -- Genetic aspects ,Proteins -- Physiological aspects ,Proteins -- Genetic aspects ,Cytoplasm -- Physiological aspects ,Cytoplasm -- Genetic aspects ,Chemical inhibitors -- Physiological aspects ,Biological sciences - Abstract
We report the development and characterization of an in vitro system for the formation of filopodia-like bundles. Beads coated with actin-related protein 2/3 (Arp2/3)--activating proteins can induce two distinct types of actin organization in cytoplasmic extracts: (1) comet tails or clouds displaying a dendritic array of actin filaments and (2) stars with filament bundles radiating from the bead. Actin filaments in these bundles, like those in filopodia, are long, unbranched, aligned, uniformly polar, and grow at the barbed end. Like filopodia, star bundles are enriched in fascin and lack Arp2/3 complex and capping protein. Transition from dendritic to bundled organization was induced by depletion of capping protein, and add-back of this protein restored the dendritic mode. Depletion experiments demonstrated that star formation is dependent on Arp2/3 complex. This poses the paradox of how Arp2/3 complex can be involved in the formation of both branched (lamellipodia-like) and unbranched (filopodia-like) actin structures. Using purified proteins, we showed that a small number of components are sufficient for the assembly of filopodia-like bundles: Wiskott-Aldrich syndrome protein (WASP)--coated beads, actin, Arp2/3 complex, and fascin. We propose a model for filopodial formation in which actin filaments of a preexisting dendritic network are elongated by inhibition of capping and subsequently cross-linked into bundles by fascin.
- Published
- 2003
24. The RasGAP-associated endoribonuclease G3BP assembles stress granules
- Author
-
Tourriere, Helene, Chebli, Karim, Zekri, Latifa, Courselaud, Brice, Blanchard, Jean Marie, Bertrand, Edouard, and Tazi, Jamal
- Subjects
Cytology -- Research ,Cytoplasm -- Physiological aspects ,Cytoplasm -- Genetic aspects ,Toxins -- Physiological aspects ,Messenger RNA -- Genetic aspects ,Metabolism -- Physiological aspects ,Phosphorylation -- Physiological aspects ,Gene expression -- Physiological aspects ,Gene mutations -- Physiological aspects ,Ribonuclease -- Genetic aspects ,Ribonuclease -- Physiological aspects ,Biological sciences - Abstract
Stress granules (SGs) are formed in the cytoplasm in response to various toxic agents, and are believed to play a critical role in the regulation of mRNA metabolism during stress. In SGs, mRNAs are stored in an abortive translation initiation complex that can be routed to either translation initiation or degradation. Here, we show that G3BP, a phosphorylation-dependent endoribonuclease that interacts with RasGAP, is recruited to SGs in cells exposed to arsenite. G3BP may thus determine the fate of mRNAs during cellular stress. Remarkably, SG assembly can be either dominantly induced by G3BP overexpression, or on the contrary, inhibited by expressing a central domain of G3BP. This region binds RasGAP and contains serine 149, whose dephosphorylation is induced by arsenite treatment. Critically, a phosphomimetic mutant (S149E) fails to oligomerize and to assemble SGs, whereas a nonphosphorylatable G3BP mutant (S149A) does both. These results suggest that G3BP is an effector of SG assembly, and that Ras signaling contributes to this process by regulating G3BP dephosphorylation.
- Published
- 2003
25. Tyrosine-phosphorylated and nonphosphorylated isoforms of [alpha]-dystrobrevin: roles in skeletal muscle and its neuromuscular and myotendinous junctions
- Author
-
Grady, R. Mark, Akaaboune, Mohammed, Cohen, Alexander L., Maimone, Margaret M., Lichtman, Jeff W., and Sanes, Joshua R.
- Subjects
Cytology -- Research ,Tyrosine -- Genetic aspects ,Tyrosine -- Physiological aspects ,Cytoplasm -- Physiological aspects ,Cytoplasm -- Genetic aspects ,Muscle cells -- Physiological aspects ,Muscle cells -- Genetic aspects ,Glycoproteins -- Physiological aspects ,Glycoproteins -- Genetic aspects ,Dystrophin -- Genetic aspects ,Dystrophin -- Physiological aspects ,Muscular dystrophy -- Causes of ,Genetic engineering -- Management ,Company restructuring/company reorganization ,Company organization ,Biological sciences - Abstract
[alpha]-Dystrobrevin (DB), a cytoplasmic component of the dystrophin--glycoprotein complex, is found throughout the sarcolemma of muscle cells. Mice lacking [alpha]DB exhibit muscular dystrophy, defects in maturation of neuromuscular junctions (NMJs) and, as shown here, abnormal myotendinous junctions (MTJs). In normal muscle, alternative splicing produces two main [alpha]DB isoforms, [alpha]DB1 and [alpha]DB2, with common N[H.sub.2]-terminal but distinct COOH-terminal domains. [alpha]DB1, whose COOH-terminal extension can be tyrosine phosphorylated, is concentrated at the NMJs and MTJs. [alpha]DB2, which is not tyrosine phosphorylated, is the predominant isoform in extrajunctional regions, and is also present at NMJs and MTJs. Transgenic expression of either isoform in [alpha]D[B.sup.-/-] mice prevented muscle fiber degeneration; however, only [alpha]DB1 completely corrected defects at the NMJs (abnormal acetylcholine receptor patterning, rapid turnover, and low density) and MTJs (shortened junctional folds). Site-directed mutagenesis revealed that the effectiveness of [alpha]DB1 in stabilizing the NMJ depends in part on its ability to serve as a tyrosine kinase substrate. Thus, [alpha]DB1 phosphorylation may be a key regulatory point for synaptic remodeling. More generally, [alpha]DB may play multiple roles in muscle by means of differential distribution of isoforms with distinct signaling or structural properties.
- Published
- 2003
26. A mechanism of coupling RCC1 mobility to RanGTP production on the chromatin in vivo
- Author
-
Li, Hoi Yeung, Wirtz, Denis, and Zheng, Yixian
- Subjects
Cytology -- Research ,Cell cycle -- Physiological aspects ,Cell cycle -- Genetic aspects ,Mitosis -- Genetic aspects ,Mitosis -- Physiological aspects ,Cytoplasm -- Physiological aspects ,Cytoplasm -- Genetic aspects ,Enzymes -- Genetic aspects ,Enzymes -- Physiological aspects ,Chromosomes -- Physiological aspects ,Chromosomes -- Genetic aspects ,Genetic regulation -- Physiological aspects ,Biological sciences - Abstract
The RanGTP gradient across the interphase nuclear envelope and on the condensed mitotic chromosomes is essential for many cellular processes, including nucleocytoplasmic transport and spindle assembly. Although the chromosome-associated enzyme RCC1 is responsible for RanGTP production, the mechanism of generating and maintaining the RanGTP gradient in vivo remains unknown. Here, we report that regulator of chromosome condensation (RCC1) rapidly associates and dissociates with both interphase and mitotic chromosomes in living cells, and that this mobility is regulated during the cell cycle. Our kinetic modeling suggests that RCC1 couples its catalytic activity to chromosome binding to generate a RanGTP gradient. Indeed, we have demonstrated experimentally that the interaction of RCC1 with the chromatin is coupled to the nucleotide exchange on Ran in vivo. The coupling is due to the stable binding of the binary complex of RCC1-Ran to chromatin. Successful nucleotide exchange dissociates the binary complex, permitting the release of RCC1 and RanGTP from the chromatin and the production of RanGTP on the chromatin surface.
- Published
- 2003
27. Evidence for involvement of the putative first extracellular loop in differential volume sensitivity of the Na (super)+/H (super)+ exchangers NHE1 and NHE2
- Author
-
Xiaohua Su, Tianxiang Pang, Wakabayashi, Shigeo, and Shigekawa, Munekazu
- Subjects
Biochemistry -- Research ,Sodium channels -- Physiological aspects ,Hydrogen -- Physiological aspects ,Gene mutations -- Physiological aspects ,Cells -- Genetic aspects ,Hydrogen-ion concentration -- Physiological aspects ,Cytoplasm -- Physiological aspects ,Cytoplasm -- Genetic aspects ,Biological sciences ,Chemistry - Abstract
Research has been conducted on PS120 cells expressing Na (super)+/H (super)+ exchanger isoforms and their mutants. The study of the hyperosmolarity-induced changes in cell volume and cytoplasmic pH in these cells is described.
- Published
- 2003
28. The role of the lissencephaly protein Pac1 during nuclear migration in budding yeast
- Author
-
Lee, Wei-Lih, Oberle, Jessica R., and Cooper, John A.
- Subjects
Cell research -- Analysis ,Mitosis -- Physiological aspects ,Mitosis -- Genetic aspects ,Brewer's yeast -- Physiological aspects ,Brewer's yeast -- Genetic aspects ,Cytoplasm -- Genetic aspects ,Cytoplasm -- Physiological aspects ,Microtubules -- Genetic aspects ,Microtubules -- Physiological aspects ,Biological sciences - Abstract
During mitosis in Saccharomyces cerevisiae, the mitotic spindle moves into the mother-bud neck via dyneindependent sliding of cytoplasmic microtubules along the cortex of the bud. Here we show that Pac1, the yeast homologue of the human lissencephaly protein LIS1, plays a key role in this process. First, genetic interactions placed Pac1 in the dynein/dynactin pathway. Second, cells lacking Pac1 failed to display microtubule sliding in the bud, resulting in defective mitotic spindle movement and nuclear segregation. Third, Pac1 localized to the plus ends (distal tips) of cytoplasmic microtubules in the bud. This localization did not depend on the dynein heavy chain Dyn1. Moreover, the Pac1 fluorescence intensity' at the microtubule end was enhanced in cells lacking dynactin or the cortical attachment molecule Num1. Fourth, dynein heavy chain Dyn1 also localized to the tips of cytoplasmic microtubules in wild-type cells. Dynein localization required Pac1 and, like Pac1, was enhanced in cells lacking the dynactin component Arp1 or the cortical attachment molecule Num1. Our results suggest that Pac1 targets dynein to microtubule tips, which is necessary for sliding of microtubules along the bud cortex. Dynein must remain inactive until microtubule ends interact with the bud cortex, at which time dynein and Pac1 appear to be offloaded from the microtubule to the cortex.
- Published
- 2003
29. Anthrax toxin triggers endocytosis of its receptor via a lipid raft-mediated clathrin-dependent process
- Author
-
Abrami, Laurence, Liu, Shihui, Cosson, Pierre, Leppla, Stephen H., and van der Goot, F. Gisou
- Subjects
Anthrax -- Physiological aspects ,Toxins -- Physiological aspects ,Antigens -- Physiological aspects ,Cytoplasm -- Genetic aspects ,Cytoplasm -- Physiological aspects ,Endocytosis -- Genetic aspects ,Endocytosis -- Physiological aspects ,Cell research -- Analysis ,Biological sciences - Abstract
The protective antigen (PA) of the anthrax toxin binds to a cell surface receptor and thereby allows lethal factor (LF) to be taken up and exert its toxic effect in the cytoplasm. Here, we report that clustering of the anthrax toxin receptor (ATR) with heptameric PA or with an antibody sandwich causes its association to specialized cholesterol and glycosphingolipid-rich microdomains of the plasma membrane (lipid rafts). We find that although endocytosis of ATR is slow, clustering it into rafts either via PA heptamerization or using an antibody sandwich is necessary and sufficient to trigger efficient internalization and allow delivery of LF to the cytoplasm. Importantly, altering raft integrity using drugs prevented LF delivery and cleavage of cytosolic MAPK kinases, suggesting that lipid rafts could be therapeutic targets for drugs against anthrax. Moreover, we show that internalization of PA is dynamin and Eps15 dependent, indicating that the clathrin-dependent pathway is the major route of anthrax toxin entry into the cell. The present work illustrates that although the physiological role of the ATR is unknown, its trafficking properties, i.e., slow endocytosis as a monomer and rapid clathrin-mediated uptake on clustering, make it an ideal anthrax toxin receptor.
- Published
- 2003
30. Dynactin is required for bidirectional organelle transport
- Author
-
Deacon, Sean W., Serpinskaya, Anna S., Vaughan, Patricia S., Fanarraga, Monica Lopez, Vernos, Isabelle, Vaughan, Kevin T., and Gelfand, Vladimir I.
- Subjects
Cell research -- Analysis ,Kinesin -- Genetic aspects ,Kinesin -- Physiological aspects ,Microtubules -- Genetic aspects ,Microtubules -- Physiological aspects ,Cell organelles -- Genetic aspects ,Cell organelles -- Physiological aspects ,Cytoplasm -- Genetic aspects ,Cytoplasm -- Physiological aspects ,Biochemistry -- Research ,Proteins -- Genetic aspects ,Proteins -- Physiological aspects ,Biological sciences - Abstract
Kinesin II is a heterotrimeric plus end-directed microtubule motor responsible for the anterograde movement of organelles in various cell types. Despite substantial literature concerning the types of organelles that kinesin II transports, the question of how this motor associates with cargo organelles remains unanswered. To address this question, we have used Xenopus laevis melanophores as a model system. Through analysis of kinesin II-mediated melanosome motility, we have determined that the dynactin complex, known as an anchor for cytoplasmic dynein, also links kinesin II to organelles. Biochemical data demonstrates that the putative cargo-binding subunit of Xenopus kinesin II, Xenopus kinesin II-associated protein (XKAP), binds directly to the p[150.sup.Glued] subunit of dynactin. This interaction occurs through aa 530-793 of XKAP and aa 600-811 of p[150.sup.Glued]. These results reveal that dynactin is required for transport activity of microtubule motors of opposite polarity, cytoplasmic dynein and kinesin II, and may provide a new mechanism to coordinate their activities.
- Published
- 2003
31. Regulation of the Bacillus subtilis bcrC bacitracin resistance gene by two extracytoplasmic function sigma factors
- Author
-
Cao, Min and Helmann, John D.
- Subjects
Bacteriology -- Research ,Bacillus subtilis -- Physiological aspects ,Bacillus subtilis -- Genetic aspects ,Genetic regulation -- Analysis ,Bacitracin -- Physiological aspects ,Bacitracin -- Genetic aspects ,Cytoplasm -- Genetic aspects ,Cytoplasm -- Physiological aspects ,Biological sciences - Abstract
Research has been conducted on Bacillus subtilis bcrC bacitracin resistance gene. Results demonstrate that this gene is important for bacitracin resistance, is controlled by sigma (super)x and sigma (super)m and is inducible by bacitracin.
- Published
- 2002
32. Associations between cytoplasmic and nuclear loci in hybridizing populations
- Author
-
Orive, Maria E. and Barton, Nicholas H.
- Subjects
Cytoplasm -- Genetic aspects ,Cytoplasm -- Physiological aspects ,Cell nuclei -- Genetic aspects ,Cell nuclei -- Physiological aspects ,Biological sciences - Abstract
We extend current multilocus models to describe the effects of migration, recombination, selection, and nonrandom mating on sets of genes in diploids with varied modes of inheritance, allowing us to consider the patterns of nuclear and cytonuclear associations (disequilibria) under various models of migration. We show the relationship between the multilocus notation recently presented by Kirkpatrick, Johnson, and Barton (developed from previous work by Barton and Turelli) and the cytonuclear parameterization of Asmussen, Arnold, and Avise and extend this notation to describe associations between cytoplasmic elements and multiple nuclear genes. Under models with sexual symmetry, both nuclear-nuclear and cytonuclear disequilibria are equivalent. They differ, however, in cases involving some type of sexual asymmetry, which is then reflected in the asymmetric inheritance of cytoplasmic markers. An example given is the case of different migration rates in males and females; simulations using 2, 3, 4, or 5 unlinked autosomal markers with a maternally inherited cytoplasmic marker illustrate how nuclear-nuclear and cytonuclear associations can be used to separately estimate female and male migration rates. The general framework developed here allows us to investigate conditions where associations between loci with different modes of inheritance are not equivalent and to use this nonequivalence to test for deviations from simple models of admixture.
- Published
- 2002
33. Evidence that Armadillo transduces Wingless by mediating nuclear export or cytosolic activation of pangolin
- Author
-
Chan, Siu-Kwong and Struhl, Gary
- Subjects
Cell research -- Analysis ,Glycoproteins -- Genetic aspects ,Secretion -- Genetic aspects ,Drosophila -- Genetic aspects ,DNA -- Genetic aspects ,Cytoplasm -- Genetic aspects ,Biological sciences - Abstract
Research has been conducted on the secreted proteins of the Wingless family. The experimental results suggest that beta-catenin may transduce Wingless protein signals via exporting TCF from the nucleus or via TCF activation in the cytoplasm.
- Published
- 2002
34. Histone chaperone ASF1 cooperates with the Brahma chromatin-remodeling machinery
- Author
-
Moshkin, Yuri M., Armstrong, Jennifer A., Maeda, Robert K., Tamkun, John W., Verrijzer, Peter, Kennison, James A., and Karch, Francois
- Subjects
Bacterial proteins -- Genetic aspects ,Histones -- Genetic aspects ,Gene mutations -- Physiological aspects ,Gene silencing -- Research ,Drosophila -- Genetic aspects ,Chromatin -- Genetic aspects ,Cell cycle -- Physiological aspects ,Cytoplasm -- Genetic aspects ,Cytoplasm -- Physiological aspects ,Biological sciences - Abstract
Research has been conducted on histone chaperone, anti-silencing function 1 protein (ASF1). Results demonstrate that Drosophila asf1 gene mutations depress silencing at heterochromatin and that ASF1 protein has cell cycle-specific cytoplasmic and nuclear localization suggesting the crucial role of this protein in chromatin assembly and Brahma chromatin-remodeling complex-mediated chromatin remodelling.
- Published
- 2002
35. The local environment at the cytoplasmic end of TM6 of the mu opioid receptor differes from those of rhodopsin and monoamine receptors: introduction of an ionic lock between the cytoplasmic ends of helices 3 and 6 by a L6.30(275)E mutation inactivates the mu opioid receptor and reduces the constitutive activity of its T6.34(279)K mutant
- Author
-
Huang, Peng, Visiers, Irache, Weinstein, Harel, and Liu-Chen, Lee-Yuan
- Subjects
Biochemistry -- Research ,Cytoplasm -- Genetic aspects ,Opioids -- Receptors ,Rhodopsin -- Physiological aspects ,Amines -- Physiological aspects ,Gene mutations -- Physiological aspects ,G proteins -- Physiological aspects ,Biological sciences ,Chemistry - Abstract
Research has been conducted on the rhodopsin and monoamine G protein-coupled receptors. The structural differences between these receptors and the mu opioid receptor and the role of these differences in the rhodopsin-like family have been investigated and the details are presented.
- Published
- 2002
36. Integrins: bidirectional, allosteric signaling machines
- Author
-
Hynes, Richard O.
- Subjects
Cell research -- Comparative analysis ,Integrins -- Genetic aspects ,Cell adhesion -- Physiological aspects ,Ligands (Biochemistry) -- Genetic aspects ,Gene expression -- Physiological aspects ,Cytoplasm -- Genetic aspects ,Biological sciences - Abstract
The author reviews the publications on adhesion receptors integrins. The topics of interest include the ability of integrins to transmit signals between their cytoplasmic domains and their extracellular ligand binding adhesion sites.
- Published
- 2002
37. The scw1 RNA-binding domain protein regulates septation and cell-wall structure in fission yeast
- Author
-
Karagiannis, Jim, Oulton, Rena, and Young, Paul G.
- Subjects
Genetics -- Research ,RNA -- Genetic aspects ,Carrier proteins -- Genetic aspects ,Plant cell walls -- Genetic aspects ,Enzymes -- Genetic aspects ,Cytoplasm -- Genetic aspects ,Cytokinesis -- Genetic aspects ,Yeast fungi -- Genetic aspects ,Suppression, Genetic -- Research ,Biological sciences - Abstract
Loss of the nonessential RNA-binding domain protein, Scw1, increases resistance to cell-wall-degrading enzymes in fission yeast. Surprisingly, scw1 null mutations also suppress the lethality of mutations (cdc11-136, cdc7-24, cdc14-118, sid1-239, sid2-250, sid3-106, sid4-A1, and mob1-1) at all levels of the sid pathway. This pathway forms part of the septation initiation network (SIN), which regulates the onset of septum formation and ensures the proper coupling of mitosis to cytokinesis. In contrast, scw[1.sup.-] mutations do not suppress ts alleles of the rng genes, cdc12 or cdc15. These mutations also prevent the formation of a septum and in addition block assembly and/or function of the contractile acto-myosin ring. sid mutants exhibit a hyper-sensitivity to cell-wall-degrading enzymes that is suppressed by loss of Scw1. Furthermore, scw[1.sup.-]-mediated rescue of sid mutants is abolished in the presence of calcofluor white, a compound that interferes with cell-wall synthesis. These data suggest that Scw1 acts in opposition to the SIN as a negative regulator of cell-wall/septum deposition. Unlike components of the SIN, Scw1 is predominantly a cytoplasmic protein and is not localized to the spindle pole body.
- Published
- 2002
38. DegS and YaeL participate sequentially in the cleavage of RseA to activate the sigma (super)E -dependent extracytoplasmic stress response
- Author
-
Alba, Benjamin M., Leeds, Jennifer A., Onufryk, Christina, Lu, Chi Zen, and Gross, Carol A.
- Subjects
Genetic research -- Analysis ,Proteins -- Genetic aspects ,Cytoplasm -- Genetic aspects ,Cells -- Genetic aspects ,Escherichia coli -- Genetic aspects ,Proteolysis -- Physiological aspects ,Genetic transcription -- Physiological aspects ,Biological sciences - Abstract
Research has been conducted on the stress response pathways. The conserved proteolytic mechanism used by mammalian and bacterial cells in activating membrane-associated transcription factors initiating intercompartmental cellular stress response is described.
- Published
- 2002
39. YaeL (EcfE) activates the sigma (super)E pathway of stress response through a site-2 cleavage of anti-sigma (super)E, RseA
- Author
-
Kanehara, Kazue, Ito, Koreaki, and Akiyama, Yoshinori
- Subjects
Genetic research -- Analysis ,Proteolysis -- Physiological aspects ,Escherichia coli -- Genetic aspects ,Metalloenzymes -- Genetic aspects ,Zinc -- Physiological aspects ,Gene expression -- Physiological aspects ,Cytoplasm -- Genetic aspects ,Biological sciences - Abstract
Research has been conducted on Escherichia coli YaeL (EcfE), a membrane-bound zinc metalloprotease which is involved in regulated intramembrane proteolysis. Results indicate that YaeL is required for the sigma (super)E extracytoplasmic stress response.
- Published
- 2002
40. Sodium dodecyl sulfate hypersensitivity of clpP and clpB mutants of Escherichia coli
- Author
-
Rajagopal, Soumitra, Sudarsan, Narashimhan, and Nickerson, Kenneth W.
- Subjects
Microbiological research -- Analysis ,Microbiology -- Environmental aspects ,Escherichia coli -- Genetic aspects ,Gene mutations -- Physiological aspects ,Sodium sulfate -- Physiological aspects ,Allergy -- Genetic aspects ,Cytoplasm -- Genetic aspects ,Biological sciences - Abstract
Research has been conducted on the hypersensitivity of Escherichia coli mutants to sodium dodecyl sulfate. Results indicate that sodium dodecyl sulfate hypersensitivity of these mutants is affected by the sodium dodecyl sulfate stress which involves formation of denaturated and aggregated proteins in bacterial cytoplasm.
- Published
- 2002
41. Cytoplasmic retraction of the amino terminus of human multidrug resistance protein 1
- Author
-
Chen, Qun, Yang, Youyun, Liu, Yang, Han, Baoguang, and Zhang, Jian-Ting
- Subjects
Biochemistry -- Research ,Proteins -- Genetic aspects ,Cytoplasm -- Genetic aspects ,Drug resistance -- Research ,Amines -- Physiological aspects ,Gene expression -- Physiological aspects ,Monoclonal antibodies -- Genetic aspects ,Biological sciences ,Chemistry - Abstract
Research has been conducted on the human multidrug resistance protein 1. The monoclonal antibody which is directed against the human multidrug resistance protein 1 amino terminus has been investigated in studying the putative extracellular amino terminus of this protein and the details are reported.
- Published
- 2002
42. Within- and between-population variation for Wolbachia-induced reproductive incompatibility in a haplodiploid mite
- Author
-
Vala, F., Weeks, A., Claessen, D., Breeuwer, J. A. J., and Sabelis, M. W.
- Subjects
Evolution -- Research ,Wolbachia -- Genetic aspects ,Reproduction -- Genetic aspects ,Haploidy -- Genetic aspects ,Population biology -- Genetic aspects ,Cytoplasm -- Genetic aspects ,Biological sciences - Abstract
Research has been conducted on Wolbachia papientis which induces cytoplasmic incompatibility. The hypothesis that the variability of cytoplasmic incompatibility induction is present in Tetranychus urticae populations has been tested and the results are discussed.
- Published
- 2002
43. The glycolytic flux in Escherichia coli is controlled by the demand for ATP
- Author
-
Koebmann, Brian J., Westerhoff, Hans V., Snoep, Jacky L., Nilsson, Dan, and Jensen, Peter R.
- Subjects
Bacteriology -- Research ,Adenosine triphosphate -- Genetic aspects ,Escherichia coli -- Genetic aspects ,Gene expression -- Physiological aspects ,Glycolysis -- Physiological aspects ,Cytoplasm -- Genetic aspects ,Biological sciences - Abstract
Research has been conducted on the nature of the glycolytic flux control. Results indicate that the expression of the F (sub)1 subunits from the H (super)+ -ATP synthase leads to the ATPase activity in Escherichia coli cytoplasm and that the added ATPase activity dramatically increases glycolysis rate.
- Published
- 2002
44. Functional interaction of region 4 of the extracytoplasmic function sigma factor FecI with the cytoplasmic portion of the FecR transmembrane protein of the Escherichia coli ferric citrate transport system
- Author
-
Mahren, Susanne, Enz, Sabine, and Braun, Volkmar
- Subjects
Bacteriology -- Research ,Cytoplasm -- Genetic aspects ,Escherichia coli -- Genetic aspects ,Membrane proteins -- Genetic aspects ,Bacterial proteins -- Genetic aspects ,Citrates -- Physiological aspects ,Iron -- Physiological aspects ,Biological sciences - Abstract
Research has been conducted on the Escherichia coli ferric citrate transport genes. The transcriptional regulation of these genes initiated by ferric citrate binding to the outer membrane protein FecA and the interaction between transmembrane protein FecR and sigma factor FecI has been investigated and discussed.
- Published
- 2002
45. Genetic locus encoding functions involved in biosynthesis and outer membrane localization of xanthomonadin in Xanthomonas oryzae pv. oryzae
- Author
-
Goel, Ajay Kumar, Rajagopal, Lakshmi, Nagesh, Narayana, and Sonti, Ramesh V.
- Subjects
Bacteriology -- Research ,Gene expression -- Physiological aspects ,Biosynthesis -- Genetic aspects ,Organic pigments -- Physiological aspects ,Bacteria -- Genetic aspects ,Cytoplasm -- Genetic aspects ,Bacterial proteins -- Genetic aspects ,Biological sciences - Abstract
Research has been conducted on the xanthomonadin, genus Xanthomonas' aryl-polyene pigments. The characterization of Xanthomonas oryzae pv. oryzae genetic locus containing open reading frames has been carried out and the results indicate that the putative cytoplasmic membrane protein encoded in this locus is required for the outer membrane localization of xanthomonadin in this bacterium.
- Published
- 2002
46. On the evolution of cytoplasmic incompatibility in haplodiploid species
- Author
-
Egas, Martin, Vala, Filipa, and Breeuwer, J. A. J., Hans
- Subjects
Evolution -- Research ,Cytoplasm -- Genetic aspects ,Population genetics -- Research ,Sex ratio -- Research ,Biological sciences - Abstract
Research has been conducted on the population dynamics and evolution in haplodiploid species' cytoplasmic incompatibility. The topics of interest include the understanding of cytoplasmic incompatibility and the effect of the threshold on the invasion by drift.
- Published
- 2002
47. Dichloromethane metabolism and C (sub)1 utilization genes in Methylobacterium strains
- Author
-
Kayser, Martin F., Ucurum, Zohre, and Vuilleumier, Stephane
- Subjects
Microbiological research -- Analysis ,Methylene chloride -- Genetic aspects ,Metabolism -- Genetic aspects ,Bacteria -- Growth ,Carbon -- Physiological aspects ,Enzymes -- Genetic aspects ,Cytoplasm -- Genetic aspects ,Gene expression -- Physiological aspects ,Plasmids -- Physiological aspects ,Biological sciences - Abstract
Research has been conducted on the ability of Methylobacterium strains to grow. The hypothesis that plasmid's dichloromethane dehalogenase expression affects the growth of Methylobacterium strains with dichloromethane as the carbon source has been tested and the results are reported.
- Published
- 2002
48. Characterization of the membrane domain Nqo11 subunit of the proton-translocating NADH-quinone oxidoreductase of Paracoccus denitrificans
- Author
-
Kao, Mou-Chieh, Di Bernardo, Salvatore, Matsuno-Yagi, Akemi, and Yagi, Takao
- Subjects
Biochemistry -- Research ,Bacteria -- Physiological aspects ,Quinone -- Physiological aspects ,Protons -- Physiological aspects ,Oxidoreductases -- Physiological aspects ,Cytoplasm -- Genetic aspects ,Biological sciences ,Chemistry - Abstract
Research has been conducted on Paracoccus denitrificans proton-translocating NADH-quinone oxidoreductase. The characterization of P. denitrificans Nqo11 subunit has been carried out and the results demonstrate that this subunit has transmembrane segments and that its C-terminus protrudes into the cytoplasmic phase.
- Published
- 2002
49. Interactions of cytoplasmic dynein light chains Tctex-1 and LC8 with the intermediate chain IC74
- Author
-
Makokha, Moses, Hare, Michael, Li, Mingang, Hays, Thomas, and Barbar, Elisar
- Subjects
Biochemistry -- Research ,Drosophila -- Physiological aspects ,Cytoplasm -- Genetic aspects ,Dynein -- Physiological aspects ,Proteolysis -- Physiological aspects ,Circular dichroism -- Analysis ,Biological sciences ,Chemistry - Abstract
Research has been conducted on the cytoplasmic dynein from Drosophila melanogaster. The dynein's subunit interactions have been characterized via the use of the limited proteolysis and circular dichroism spectroscopy and the results suggest that these subunits can regulate dynein complex assembly.
- Published
- 2002
50. Nuclear lamins: building blocks on nuclear architecture
- Author
-
Godman, Robert D., Gruenbaum, Yosef, Moir, Robert D., Shumaker, Dale K., and Spann, Timothy P.
- Subjects
Genetic research -- Analysis ,Cytoplasm -- Genetic aspects ,Chromatin -- Genetic aspects ,DNA -- Genetic aspects ,Nuclear membranes -- Genetic aspects ,Biological sciences - Abstract
Research has been conducted on the nuclear lamins, the main components of the nuclear lamina found at the chromatin and inner nuclear membrane interface. Results indicate that these lamins are the progenitors of the cytoplasmic intermediate filaments.
- Published
- 2002
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