1. shRNA transgenic swine display resistance to infection with the foot-and-mouth disease virus
- Author
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Xiangtao Liu, Yuhang Wang, Haixue Zheng, Ning Li, Wenping Hu, Qiuyan Li, Wenhai Feng, Xiaoxiang Hu, Wenjie Liu, Chen Zhisheng, Cai Xuepeng, and Shen Liu
- Subjects
Swine ,viruses ,Science ,animal diseases ,Viremia ,Article ,Virus ,Animals, Genetically Modified ,Small hairpin RNA ,Immune system ,RNA interference ,medicine ,Animals ,RNA, Small Interfering ,Disease Resistance ,Swine Diseases ,Multidisciplinary ,biology ,medicine.disease ,biology.organism_classification ,Virology ,Foot-and-Mouth Disease Virus ,Foot-and-Mouth Disease ,Genetic engineering ,biology.protein ,RNA, Viral ,Medicine ,RNA Interference ,Foot-and-mouth disease virus ,Antibody ,CD8 ,Cloning - Abstract
Foot-and-mouth disease virus (FMDV) is one of the most important animal pathogens in the world. FMDV naturally infects swine, cattle, and other cloven-hoofed animals. FMD is not adequately controlled by vaccination. An alternative strategy is to develop swine that are genetically resistant to infection. Here, we generated FMDV-specific shRNA transgenic cells targeting either nonstructural protein 2B or polymerase 3D of FMDV. The shRNA-positive transgenic cells displayed significantly lower viral production than that of the control cells after infection with FMDV (P +, CD4+, CD8+, CD21+, and CD172+ cells, and the production of IFN-γ were analyzed, there were no significant differences observed between the TGCS and NS or Non-TGCS, suggesting that the FMDV resistance may be mainly derived from the RNAi-based antiviral pathway. Our work provides a foundation for a breeding approach to preventing infectious disease in swine.
- Published
- 2021