29 results on '"Jana Petrová"'
Search Results
2. The Impact of Growth-Promoting Streptomycetes Isolated from Rhizosphere and Bulk Soil on Oilseed Rape (Brassica napus L.) Growth Parameters
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Ladislav Ducsay, Jana Maková, Jana Petrová, Juraj Medo, Soňa Javoreková, Nikola Lipková, and Renata Cinkocki
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Siderophore ,oilseed rape ,Geography, Planning and Development ,Bulk soil ,Brassica ,TJ807-830 ,Management, Monitoring, Policy and Law ,TD194-195 ,Streptomyces ,Renewable energy sources ,03 medical and health sciences ,GE1-350 ,030304 developmental biology ,0303 health sciences ,Rhizosphere ,biology ,Environmental effects of industries and plants ,030306 microbiology ,Renewable Energy, Sustainability and the Environment ,Inoculation ,fungi ,food and beverages ,biology.organism_classification ,Environmental sciences ,Horticulture ,Seedling ,PGPS ,Shoot - Abstract
Inoculation of Streptomyces to improve oilseed rape (Brassica napus L.) yields and minimise the use of chemical fertilisers is a promising sustainable strategy. In this study, we isolated 72 actinobacterial strains from rhizosphere of oilseed rape and maize and from bulk soil for screening and characterising their antimicrobial activity. Nine promising strains, identified as Streptomyces sp. by morphology, physiological characteristics, and 16S rRNA gene sequencing, were selected for their plant growth-promoting traits and in planta experiments. The actinobacterial strains were positive for IAA production, siderophore production, and HCN production. In planta experiments were conducted by soaking the oilseed rape seeds in the actinobacterial suspension, followed by plant growth under controlled conditions in a cultivate chamber (22–28 °C, 8 h dark/16 h light, constant humidity 80%). We recorded root and shoot length (cm) and seedling fresh weight (g). For most of the abovementioned parameters, a significant enhancement was observed with strain KmiRC20A118 treatment. The length of the root increased by 53.14%, the shoot length increased by 65.6%, and the weight of the fresh plant by 60% compared to the control. The integrated application of PGPS (Plant Growth Promoting Streptomyces) from the rhizosphere of oilseed rape is a promising strategy to improve the growth of oilseed rape.
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- 2021
3. IN VITRO SCREENING OF ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF MEDICINAL PLANTS GROWING IN SLOVAKIA
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Lukáš Hleba, Jana Petrová, Eva Ivanišová, Miroslava Kačániová, Sabina Gayibova, Tomáš Tóth, Takhir Aripov, Marek Slávik, Július Árvay, and Miroslava Hŕstková
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Antioxidant ,Traditional medicine ,Polyphenol ,medicine.medical_treatment ,medicine ,Biology ,Antimicrobial ,Medicinal plants ,Molecular Biology ,Microbiology ,In vitro ,Food Science ,Biotechnology - Abstract
Traditional medicine usage at the preventive medicine level is comprehensive and plant-based remedies are being prescribed for the treatment of a large scale of diseases by indigenous medical practitioners around the world. The phytochemicals being observed in the fruits and vegetables as well as herbal plants are getting investigated intensively as their important role in the avoidance of diverse human diseases is undoubtful. Antioxidant and antimicrobial activities of selected medicinal herbs were evaluated in this study. Aerial parts of plants from Hypericaceae, Lamiaceae, Fabaceae, Plantaginaceae, Rubiaceae growing wild in Slovakia (locality Zobor) were investigated to characterize their polyphenol content, flavonoid content, as well as antioxidant and antimicrobial activity. Inspection of the metabolic profile combined with HPLC data showed that the ethanol extract contained mainly eight polyphenols with apigenin, daidzein, kaempferol, rutin, quercetin, vitexin, and cynaroside as the main chemicals. Among them, neochlorogenic acid, chlorogenic acid, trans-p-caffeic acid, trans-p-coumaric acid, trans-p-sinapic acid, trans-p-ferulic acid, rosmarinic acid were identified. The antioxidant capacity of ethanol extracts was evaluated by employing DPPH radical scavenging and phosphomolybdenum assays. All samples were also tested against Gram-positive (Staphylococcus aureus, Bacillus thuringiensis, Micrococcus luteus, Staphyloccocus epidermidis) and Gram-negative (Citrobacter koseri, Escherichia coli, Pseudomonas proteolytica, Hafnia alvei, Salmonella enterica, Yersinia enterocolitica) bacterial species using the disk diffusion and minimum inhibitory concentration assays
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- 2019
4. SOIL AMENDED WITH ORGANIC FERTILIZERS AS A SOURCE OF ACTINOMYCETES WITH HIGH POTENTIAL AS BIOCONTROL AGENTS
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Lukáš Hleba, Silvia Kovácsová, Daniela Košťálová, Juraj Medo, Jana Petrová, Renata Cinkocki, Soňa Javoreková, and Jana Maková
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Compost ,Biological pest control ,engineering.material ,Biology ,Pathogenicity ,Microbiology ,Fungicide ,Agronomy ,engineering ,Natural enemies ,Molecular Biology ,Vermicompost ,High potential ,Food Science ,Biotechnology - Abstract
Plant diseases caused by pathogenic fungi are a severe problem for agriculture, and some organic fertilizers have shown a suppressive capacity due to the antagonistic action of microbial communities inhabiting those substrates. The purpose of this work was to isolate and identify actinomycetes from soil, compost and vermicompost able to antagonize phytopathogens. In total, out of 352 actinomycetes successfully recovered on Pochon medium and of which were selected representative 22 mycelium forming isolates, which were subjected to morphological and genotypic characterization. Genetic characterization based on 16S rDNA fragment sequencing revealed that, all the actinomycete isolates belong to the genus Streptomyces. The antifungal activity of isolates was tested against eight phytopathogenic fungi, and they were most activated against Rhizoctonia solani, Alternaria tenuissima, Aspergillus niger and Penicillium expansum. Isolates 51VK13 (Streptomyces sampsonii) and 12VK13 (Streptomyces flavovariabilis) isolated from soil amended with vermicompost showed the greatest antagonistic activity. Their bioactive potential was also confirmed by presence of genes for nonribosomal peptide synthetase (NRPS) and polyketide synthase types I and II (PKS-I and PKS-II). It can, therefore, be concluded that soil amended with organic fertilizers such as compost, and in particular vermicompost, provides a lower incidence of phytopathogens, especially fungi. Isolated actinomycetes have also shown high potential for the production of bioactive compounds useful in the control of plant diseases.
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- 2019
5. Growth Promotion of Rapeseed (Brassica napus L.) and Blackleg Disease (Leptosphaeria maculans) Suppression Mediated by Endophytic Bacteria
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Jana Petrová, Jana Maková, Nikola Lipková, Renata Artimová, Jaroslav Michalko, Soňa Javoreková, and Juraj Medo
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Siderophore ,Rapeseed ,biology ,Bacillus pumilus ,fungi ,Blackleg ,Brassica ,food and beverages ,Agriculture ,plant growth promoting ,biology.organism_classification ,Brassica napus ,Microbiology ,Crop ,endophytic bacteria ,Leptosphaeria maculans ,biocontrol ,Agronomy and Crop Science ,Microbial inoculant - Abstract
Rapeseed is an important oil crop strongly dependent on high agrochemical inputs. Some pathogens, including Leptosphaeria maculans, cause blackleg disease and can drastically decrease yields. Microbial inoculants seem to be a promising solution to these problems. However, a selection of potent bacterial strains able to improve growth and/or suppress disease is needed. Endophytic bacteria (n = 38) isolated from rapeseed plants with exceptionally good growth were screened for plant growth promoting (PGP) traits and L. maculans antifungal activity. A majority of isolates (35) showed the ability to produce siderophores, 17 isolates solubilized phosphate, and 28 isolates inhibited the growth of L. maculans. The six most promising isolates belonging to Bacillus genera were characterized in detail and compared to two previously published PGP strains. Plant growth measured as total weight and root length of rapeseed seedlings was stimulated by all isolates in comparison to control. The best isolate, 1L6, preliminary identified as Bacillus pumilus showed the highest phosphate solubilization, IAA and HCN production, and growth promotion of plants. Isolates with high antifungal activity in screening showed good potential to suppress disease on plants, with 87% reduction of lesions caused by L. maculans. These strains are good candidates to be explored under field use either solely or in combination.
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- 2021
6. MICROBIOTA OF PINUS POLLEN AS ADJUVANT FACTOR OF ALLERGY
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Ján Brindza, Tetiana Shevtsova, Kateryna Garkava, Miroslava Kačániová, and Jana Petrová
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Allergy ,010504 meteorology & atmospheric sciences ,medicine.medical_treatment ,lcsh:QR1-502 ,Pinus species ,Biology ,medicine.disease_cause ,01 natural sciences ,Microbiology ,lcsh:Microbiology ,03 medical and health sciences ,0302 clinical medicine ,Pollen contamination ,Pollen ,Botany ,microbiota ,medicine ,microbiological quality ,Molecular Biology ,0105 earth and related environmental sciences ,flowering ,food and beverages ,pollen contamination ,Microbiological quality ,allergy ,medicine.disease ,%22">Pinus ,030228 respiratory system ,Immunology ,Adjuvant ,Food Science ,Biotechnology - Abstract
Bacteria, their endotoxin and mold found on pollen can be a reason of respiratory symptoms in sensitized individuals. This question concerns an anemophilous pollen more acute. In this work quantitative by dilution plating method and qualitative microbial analysis by MALDI-TOF MS Biotyper of pollen and other plants organs of Pinus sylvestris L., P. nigra Arnold, P. mugo Turra, P. armandii Franch., P. wallichiana A.B. Jacks from Nitra, Slovakia are performed which shows quantitative and species differences in mesophilic aerobic (0.00-6.27 log cfu/g) and anaerobic bacteria (0.00-3.70 log cfu/g), enterococci (0.00 log cfu/g), coliform bacteria (0.00-5.29 log cfu/g), lactobacilli (0.00-4.20 log cfu/g), microscopic fungi and yeasts (2.60-5.29 log cfu/g) content. Representatives of Pseudomonas (14), Bacillus (2), Acinetobacter (1), Arthrobacter (1), Pantoea (1), Klebsiella (1), Penicillium (6), Aspergillus (4), Cladosporium (1), Debaryomyces (1) genera were revealed on pine trees. The allergenic potential of the identified association of microorganisms on pollen has been evaluated based on published data. The results may be useful for aerobiologists, allergists and microbiologists, at least at the local level.
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- 2016
7. Microbial Diversity of Betula Trees: Pollen, Catkins, Leaves Relatively of Flowering
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Kateryna Garkava, Tetiana Shevtsova, Ján Brindza, Miroslava Kačániová, and Jana Petrová
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0301 basic medicine ,medicine.disease_cause ,lcsh:Agriculture ,03 medical and health sciences ,Pollen ,Botany ,microbiota ,medicine ,microbiological quality ,Cultivar ,lcsh:QH301-705.5 ,flowering ,biology ,fungi ,lcsh:S ,food and beverages ,pollen contamination ,biology.organism_classification ,Pantoea agglomerans ,Yeast ,Coliform bacteria ,030104 developmental biology ,lcsh:Biology (General) ,Catkin ,Betula verrucosa Ehrh ,Bee pollen ,General Agricultural and Biological Sciences ,Mesophile - Abstract
Quantitative microbiological analysis by dilution plating method of pollen and additional male and female catkins, leaves of Betula verrucosa Ehrh. and its two cultivars: ‘Purpurea’ and ‘Youngii’ relatively of flowering period of Betula has been realized with the aim to provide new knowledge of the microbiological quality of anemophilous pollen for processing and its further application. Qualitative microbiological analysis with MALDI-TOF MS Biotyper was used in the identification of aerobic, anaerobic mesophilic bacteria and coliforms. Mixed microbiota was determined, consisting of aerobic (4.68–4.89 log cfu/g) and anaerobic (3.30–3.48 log cfu/g) mesophilic bacteria, lactobacilli (0–3.48 log cfu/g), coliform bacteria (0–4.57 log cfu/g), fungi and yeast (3.78–3.95 log cfu/g) on the pollen grains, that indicates acceptable quality in comparison with the microbiological quality parameters for bee pollen. Pantoea agglomerans was found associated with pollen of Betula verrucosa Ehrh. Recommendations on the collection of anemophilous pollen were established.
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- 2016
8. ANTIMICROBIAL ACTIVITY OF CRUDE ETHANOLIC EXTRACTS FROM SOME MEDICINAL MUSHROOMS
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Lukáš Hleba, Jana Petrová, Miroslava Kačániová, Martin Rajtar, Jana Hutková, Attila Kántor, Matúš Kompas, and Juraj Čuboň
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0301 basic medicine ,Gram-negative bacteria ,Traditional medicine ,Gram-positive bacteria ,030106 microbiology ,Biology ,010402 general chemistry ,biology.organism_classification ,Antimicrobial ,01 natural sciences ,Microbiology ,0104 chemical sciences ,03 medical and health sciences ,chemistry.chemical_compound ,chemistry ,Medicinal fungi ,Molecular Biology ,Food Science ,Biotechnology - Abstract
In this paper the antimicrobial activity of 1 year old crude ethanolic extracts obtained from Cordyceps sinesis, Laricifomes officinalis, Oudemansiella mucida and Coprinus comatus were investigated. The antimicrobial activities of extracts against two Gram-positive bacteria (Bacillus thuringiensis, Staphylococcus aureus) and two Gram-negative bacteria (Klebsiella pneumoniae, Enterobacter aerogenes) were determined by disk diffusion and microbroth dilution method according by EUCAST in 96-well microplates. Microorganisms were obtained from Czech Collection of Microorganisms. Absorbance after and before the experiment were substracted, converted to binary system and obtained values to Probit analysis were used. Not all macromycetes ethanolic extracts showed antimicrobial activity against tested bacteria. Antimicrobial activity determined by MIC methodology showed extracts from Oudemansiella mucida, Cordyceps sinesis, Coprinus comatus in the tested range. Conversely, the best antimicrobial activity tested by disc diffusion methods showed extract from Laricifomes officinalis. Equally, more better studying of antimicrobial activity in these mushrooms will needed.
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- 2016
9. Microbiological quality of chicken thighs meat after four essential oils combination, EDTA and vaccum packing
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Jana Hutková, Rafal Kordiaka, Simona Kunová, Attila Kántor, Maciej Kluz, Juraj Čuboň, Czesław Puchalski, Margarita Terentjeva, Miroslava Kačániová, Martin Mellen, Peter Haščík, and Jana Petrová
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Preservative ,Food industry ,lcsh:TX341-641 ,Vacuum packing ,law.invention ,meat ,chemistry.chemical_compound ,0404 agricultural biotechnology ,vaccum ,law ,Pimpinella anisum ,Botany ,Food science ,microorganisms ,essential oils ,Essential oil ,biology ,business.industry ,EDTA ,04 agricultural and veterinary sciences ,Origanum ,biology.organism_classification ,Antimicrobial ,040401 food science ,Lactic acid ,chemistry ,business ,lcsh:Nutrition. Foods and food supply ,Food Science - Abstract
The aim of the present work to monitoring chicken the microbiological quality of vaccum packaged thighs after treatment by ethylenediaminetetraacetate (EDTA), anise (Pimpinella anisum), spearmint (Mentha spicata var. crispa), thyme (Thymus vulgaris L.) oregano (Origanum vulgare L.) essential oils and stored in at 4 ±0.5 °C for a period of 16 days. The following treatments of chicken thighs were used: air-packaged control samples, control vacuum-packaged samples, vacuum-packaging with EDTA solution 1.5% w/w, control samples, vacuum-packaging after treatment with Pimpinella anisum, Mentha spicata var. crispa essential oil at concentrations 0.2% v/w, vacuum-packaging after treatment with Thymus vulgaris L., Origanum vulgare L. essential oil at concentration 0.2% v/w. The quality assessment of all samples was done microbiologically and following microbiological parameters were detected: the anaerobic plate count, Enterobacteraceae counts, lactic acid bacteria and Pseudomonas spp. counts. The number of anaerobic plate count ranged from 3.69 log CFU.g-1 in all tested group on 0 day to 5.68 log CFU.g-1 on 16 day in control group stored in air condition. The number of lactic acid bacteria ranged from 2.00 log CFU.g-1 in all tested group on 0 day to 4.82 log CFU.g-1 on 16 day in group with oregano, thyme essential oils combination. Enterobacteriacea counts in chicken thighs was 0.68 log CFU.g-1 on 0 day to 7.58 CFU.g-1 on 16 day in air-packed meat samples. The Pseudomonas spp. was not found in all tested samples. Among the antimicrobial combination treatments examined in this work, the as application of vacuum packaging, EDTA and essential oils treatment was the most effective against the growth of Enterobactericeae, inhibitory effect on anaerobic plate count also was observed. The results of this present study suggest the possibility of application the Pimpinella anisum, Mentha spicata var. crispa, Thymus vulgaris L., Origanum vulgare L. essential oil of as natural food preservatives and potential sources of antimicrobial ingredients for food industry for chicken thighs meat treatment.
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- 2016
10. Indicience of bacteria nad antibacterial activity of selected types of tea
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Miroslava Kačániová, Jana Petrová, Margarita Terentjeva, Maciej Kluz, Attila Kántor, Czesław Puchalski, Jana Hutková, Simona Kunová, and Rafal Kordiaka
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Klebsiella ,tea ,Gram-positive bacteria ,MALDI TOF MS Biotyper ,food and beverages ,lcsh:TX341-641 ,Total Viable Count ,Pathogenic bacteria ,04 agricultural and veterinary sciences ,Biology ,Antimicrobial ,biology.organism_classification ,medicine.disease_cause ,040401 food science ,Enterococcus durans ,Microbiology ,Minimum inhibitory concentration ,0404 agricultural biotechnology ,antibacterial activity ,medicine ,Food science ,bacteria ,Antibacterial activity ,lcsh:Nutrition. Foods and food supply ,Food Science - Abstract
The purpose of this study was to determine in vitro antibacterial activity of selected teas (Assam: Indian black tea fromCamellia sinensis, Pu-erh: darkpu-erh (shu) fromCamellia sinensis, Sencha: Japanese green tea from Camellia sinensis) against five species of pathogenic microorganisms. In our study, we determined the total viable count (TVC), number of yeasts (Y) and number of Enterobacteriaceae genera (E). Then MALDI-TOF MS Biotyper identified colonies after cultivation. Evaluation of the antimicrobial activity was performed by disc diffusion method, well diffusion method and detection of minimum inhibitory concentration (MIC). For antibacterial activity, bacteria Escherichia coli CCM 2024, Yersinia enterocolitica CCM 5671, Klebsiella pneumonie CCM 2318, Staphylococus aureus CCM 2461 and Bacillus thurigiensis CCM19 were used. The inhibition zones were measured in mm in disc diffusion method and well diffusion method. The MIC of the individual extracts was measured spectrophotometrically. The high number of total viable count was found in Pu-erh tea (2.1 log CFU.g-1) and lowest number was found in Assam tea (0.7 log CFU.g-1). The high number of Enterobacteriacea genera was found in Pu-erh tea (2.03 log CFU.g-1) and lowest number was found in Assam tea (0 log CFU.g-1). The higher number of yeasts was found in Pu-erh tea (1.83 log CFU.g-1) and lowest number was found in Assam tea (0.3 log CFU.g-1). Mass spectrometry revealed seven Gram positive bacteria Bacillus cereus, B. mycoides, B. pumilus, Enterococcus durans, Staphylococcus epidermis, S. hominis, S. warneri, four Gram negative bacteria Acinetobacter junii, Hafnia alvei, Klebsiella pneumoniae, Sphingomonas spp. andtwo yeast Candida glabrata, Cryptococcus albidus. The results show that certain tea extracts are particularly active against various pathogenic bacteria. Tea extracts (Sencha, Rooibos, Mate, Assam) were found to have the strongest antibacterial activity against bacteria Staphylococcus aureus CCM 2461.
- Published
- 2016
11. The extension of shelf-life of chicken meat after application of caraway and anise essential oils and vacuum packaging
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Czeslav Puchalski, Jana Hutková, Simona Kunová, Miroslava Kačániová, Peter Haščík, Margarita Terentjeva, Jana Petrová, Martin Mellen, Juraj Čuboň, Rafal Kordiaka, Attila Kántor, and Maciej Kluz
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Preservative ,Gram-positive bacteria ,vacuum ,lcsh:TX341-641 ,Vacuum packing ,Shelf life ,law.invention ,chemistry.chemical_compound ,0404 agricultural biotechnology ,law ,Food science ,bacteria ,Essential oil ,biology ,Food preservation ,EDTA ,04 agricultural and veterinary sciences ,chicken breast ,caraway and anise essential oils ,Antimicrobial ,biology.organism_classification ,040401 food science ,Lactic acid ,chemistry ,lcsh:Nutrition. Foods and food supply ,Food Science - Abstract
The effect of caraway (CEO) and anise (AEO) essential oils as well as vacuum packaging (VP) in extending of the shelf life of fresh chicken breast meat stored at 4 °C was investigated. CEO and AEO were used at concentrations 0.2% v/w with and without VP. Microbiological properties of chicken breast meat were monitored over a 16 day period. The microbiological parameters as the anaerobic plate count (AC), Enterobacteraceae, lactic acid bacteria and Pseudomonas spp. counts were detected. The anaerobic plate counts ranged from 2.77 log cfu.g-1 in all tested group on 0 day to 5.45 log cfu.g-1 on 16 day in control group stored in air condition. The number of lactic acid bacteria ranged from 3.20 log cfu.g-1 in all tested group on 0 day to 4.75 log cfu.g-1 on 16 day in control group stored in air condition. Enterobacteriaceae counts ranged from 0.00 to 4.25 log cfu.g-1 on 16 day in control group stored in air condition. The number of Pseudomonas spp. ranged from 0.00 log cfu.g-1 in all tested group on 0 day to 2.65 log cfu.g-1 on 16 day in control group stored in air condition. Statistically significant differences (P≤0.001) were found among tested group in all tested microorganisms. Among the antimicrobial combination treatments were examined in the study, the as application of vacuum packaging, EDTA, and essential oils were the most effective against the growth of lactic acid bacteria and Enterobactericeae and to a less extent on anaerobic plate count. The results of this present study suggest the possibility of using the essential oil of caraway and anise as natural food preservatives and potential source of antimicrobial ingredients for chicken breast meat.
- Published
- 2016
12. Yeast diversity in new, still fermenting wine 'federweisser'
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Jana Petrová, Attila Kántor, Miroslava Kačániová, and Jana Hutková
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0301 basic medicine ,Wine ,new wine ,food.ingredient ,biology ,030106 microbiology ,yeasts ,lcsh:TX341-641 ,Saccharomyces cerevisiae ,biology.organism_classification ,Yeast ,Pichia occidentalis ,03 medical and health sciences ,Horticulture ,food ,Hanseniaspora uvarum ,Botany ,Agar ,MALDI-TOF MS ,Fermentation ,Malt extract agar ,lcsh:Nutrition. Foods and food supply ,Metschnikowia pulcherrima ,Food Science - Abstract
The aim of this study was to isolate and identify yeasts in different new wine "federweisser" samples. We collected the samples at the end of the August 2015 and in the middle of the September 2015. Used 15 new wine samples in this study (5 white and 10 red) were from the local Slovak winemakers. Irsai Oliver (3), Moravian Muscat (2), Agria/Turan (1), Dornfelder (3), Blue Frankish (3), Pinot Noir (1) and Saint Laurent (2). Three cultivation media were used for detection of yeasts in "federweisser" samples. Malt extract agar base (MEA), Wort agar (WA) and Wild yeast medium (WYM) were used for the cultivation of yeasts. Cultivation was performed by spread plate method. Ethanol/formic acid extraction procedure was used for preparation of samples. MALDI-TOF Mass Spectrometer (Microflex LT/SH) (Bruker Daltonics, Germany) was used for the identification of yeasts. We identified seven different strains of Saccharomyces cerevisiae (23; 70%), two strains of Kloeckera apiculata [teleomorph Hanseniaspora uvarum] (7; 21%), and one strain of Pichia kluyveri (1; 3%), Pichia occidentalis [anamorph Candida sorbosa] (1; 3%) and Metschnikowia pulcherrima (1; 3%) in 15 new wine "federweisser" samples. Saccharomyces cerevisiae was dominant species in each new wine sample, and formed creamy convex colonies with circular edge. Metschnikowia pulcherrima formed convex to pulvinate, circular white-pink colored colonies, Kloeckera apiculata formed flat, circular smooth colonies with turquoise center with gray edge, Pichia occidentalis formed irregular pulvinate light-cream colored colonies, and Pichia kluyveri formed turquoise, convex, undulate and smooth colonies on Malt extract agar base with bromocresol green.  
- Published
- 2016
13. Application of lavender and rosemary essential oils improvement of the microbiological quality of chicken quarters
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Margarita Terentjeva, Jana Petrová, Attila Kántor, Miroslava Kačániová, Maciej Kluz, Czesław Puchalski, Martin Mellen, Jana Hutková, Juraj Čuboň, Peter Haščík, Simona Kunová, and Rafal Kordiaka
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food.ingredient ,Lavender ,vacuum ,EDTA ,lcsh:TX341-641 ,Vacuum packing ,Plate count agar ,Biology ,law.invention ,Microbiology ,Lactic acid ,chickens quarters ,chemistry.chemical_compound ,food ,chemistry ,law ,lavender and rosemary essential oils ,Agar ,Food science ,microorganisms ,lcsh:Nutrition. Foods and food supply ,Anaerobic exercise ,Incubation ,Essential oil ,Food Science - Abstract
The aim of the present work was monitoring of chicken quarters microbiological indicators after treatment by ethylenediaminetetraacetate (EDTA), lavender (Lavandula angustifolia L.) and rosemary (Rosmarinus officinalis L.) essential oil, stored under vacuum packaging, at 4 ±0.5°C for a period of 16 days. The following treatments of chicken quarters were used: Air-packaging control samples, control vacuum-packaging samples, vacuum-packaging with EDTA solution 1.50% w/w, control samples, vacuum-packaging with Lavandula angustifolia essential oil at concentrations 0.2% v/w and vacuum-packaging with Rosmarinus officinalis essential oil at concentration 0.2% v/w. The quality assessment of all samples was established by microbiological analysis. Sampling was carried out after certain time intervals: 0, 4, 8, 12 and 16 days. Chicken quarters were stored under vacuum packaging, at 4 ±0.5°C during experiment. Microbiological analyses were conducted by using standard microbiological methods. Anaerobic plate count were determined using Plate Count Agar, after incubation for 2 days at 35°C under anaerobic condition. Pseudomonas spp. were determined on Pseudomonas Isolation agar after incubation at 48 h at 25°C. For lactic acid bacteria were inoculated into Rogosa and Sharpe agar after incubation 48-78 h at 37°C in an aerobic atmosphere supplemented with carbon dioxide (5% CO2). For members of the family Enterobacteriaceae violet red bile glucose agar were used and samples were incubated at 37°C for 24 h. The initial APC value of chicken quarter was 3.00 log CFU.g-1 on 0 day. The number of anaerobic plate count ranged from 3.00 log CFU.g-1 in all tested group on 0 day to 6.11 log CFU.g-1 on 16 day in control group stored in air condition. The initial LAC value of chicken quarter was 3.00 log CFU.g-1 on 0 day. The number of lactic acid bacteria ranged from 3.00 log CFU.g-1 in all tested group on 0 day to 3.58 log CFU.g-1 on 16 day in control group stored in air condition. The initial Enterobacteriacea genera value of chicken quarter was 2.00 log CFU.g-1 on 0 day. Presences of these bacteria were found on all groups at 16 days. The results of this present study suggest the possibility of application the Lavandula angustifolia and Rosmarinus officinalis essential oil as natural food preservatives and potential sources of antimicrobial ingredients for food industry. 
- Published
- 2015
14. Bee bread - perspective source of bioactive compounds for future
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Jana Petrová, Valeryii Brovarskyi, Miroslava Kačániová, Leonora Adamchuk, Serhii Velychko, Jana Hutková, Eva Ivanišová, Janette Musilová, Helena Frančáková, and Zuzana Schubertová
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Antioxidant ,DPPH ,medicine.medical_treatment ,Flavonoid ,Trolox equivalent antioxidant capacity ,antioxidant activity ,lcsh:TX341-641 ,Biology ,chemistry.chemical_compound ,Botany ,medicine ,Food science ,Gallic acid ,polyphenols ,chemistry.chemical_classification ,antimicrobial activity ,fungi ,food and beverages ,chemistry ,Polyphenol ,Bee pollen ,pollen ,flavonoids ,Quercetin ,lcsh:Nutrition. Foods and food supply ,Food Science - Abstract
Bee bread is product with long history used mainly in folk medicine. Nowadays, bee bread is growing in commercial interest due to its high nutritional properties. The objective of this study was to determine biological activity of ethanolic extract of bee bread obtained from selected region of Ukraine - Poltava oblast, Kirovohrad oblast, Vinnica oblast, Kyiv oblast, Dnepropetrovsk oblast. The antioxidant activity was measured with the radical scavenging assays using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical as well as phosphomolybdenum assay. Total polyphenol content was determined with Folin-Ciocalteau reagent and total flavonoid content by aluminium-chloride method. Secondary was also evaluated antimicrobial activity in bee bread samples with disc diffusion method and minimum inhibitory concentrations. Antioxidant activity expressed as mg TEAC per g of dry weight (Trolox equivalent antioxidant capacity) was the highest in bee bread from Poltava oblast in DPPH and also phosphomolybdenum method. Samples of bee bread contained high levels of total polyphenols (12.36 - 18.24 mg GAE - gallic acid equivalent per g of dry weight) and flavonoids (13.56 - 18.24 μg QE - quercetin equivalent per g of dry weight) with the best values of bee bread from Poltava oblast. An elevated level of antioxidant potential in the bee bread determines its biological properties, which conditioned of the biological active substances. The best antibacterial activity of bee bred with disc diffusion method was found against Bacillus thuringiensis CCM 19. The antibacterial activity inhibited by the bee bread extract in the present study indicate that best minimal inhibition concentration was against bacteria Escherichia coli CCM 3988 and Salmonella enterica subs. enterica CCM 3807.
- Published
- 2015
15. ANTIMICROBIAL ACTIVITY OF PULCHERRIMIN PIGMENT PRODUCED BY METSCHNIKOWIA PULCHERRIMA AGAINST VARIOUS YEAST SPECIES
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Lukáš Hleba, Miroslava Kačániová, Attila Kántor, Jana Hutková, and Jana Petrová
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0301 basic medicine ,biology ,030106 microbiology ,biology.organism_classification ,Antimicrobial ,Microbiology ,Yeast ,03 medical and health sciences ,Pigment ,visual_art ,Botany ,visual_art.visual_art_medium ,Molecular Biology ,Metschnikowia pulcherrima ,Food Science ,Biotechnology - Abstract
Metschnikowia pulcherrima is common yeast on grape berries and can grow on special cultivation media with iron (e.g. FeSO4.7H2O) (max. 0.02 g/L) to produce reddish (maroon) pigment pulcherrimin. Many studies confirm that pulcherrimin has antimicrobial activity against postharvest pathogens, microscopic fungi and other yeast species. In this study, two strains of M. pulcherrima, isolated from grapes and identified by MALDI-TOF mass spectrometry, were used : VML and CBS 610NT. Sixteen yeast species: Aureobasidium (1), Candida (5), Hanseniaspora (1), Kregenvanrija (1), Pichia (2), Rhodotorula (2), Saccharomyces (1), Zygosaccharomyces (2) and Wickerhamomyces (1) were selected to test the antimicrobial capacity of these two M. pulcherrima strains. The results showed CBS 610NT had a stronger ability than VML to inhibit the tested yeasts. Among the yeast, Candida spp. and Pichia manshurica were the most sensitive to the pigment pulcherrimin.
- Published
- 2015
16. THE MICROBIOLOGICAL QUALITY OF ANEMOPHILOUS POLLEN WITH ALLERGENIC POTENTIAL AFTER COLLECTION AND STORAGE
- Author
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Tetiana Shevtsova, Jana Petrová, Miroslava Kačániová, Ján Brindza, Kateryna Garkava, and Rafal Kordiaka
- Subjects
%22">Pinus ,Pollen ,Botany ,Anemophily ,medicine ,Microbiological quality ,Biology ,medicine.disease_cause ,Molecular Biology ,Microbiology ,Food Science ,Biotechnology ,Woody plant - Abstract
The aim of this study is to determine and compare microbiological contamination of anemophilous pollens (Betula verrucosa Ehrh., Betula verrucosa Ehrh. 'Youngii', Pinus sylvestris L., Pinus nigra Arnold, Pinus mugo Turra, Pinus armandii Franch., Pinus wallichiana A.B. Jacks) after collection and after not long-term storage. Samples were picked by hands aseptically in the territory of the same environmental conditions in Slovakia. Colonies of mesophilic aerobic and anaerobic bacteria, lactobacilli, total coliforms, fungi and yeast were found on pollen samples by dilution plating method. It was found significant differences in content of lactobacilli for birch pollens and fungi and yeast on pine pollens, depending on the stages – collection and storage. The microbiological quality of Betula pollen is better than Pinus pollen after collection and storage. Species factor affects the microbiota of anemophilous pollen.
- Published
- 2015
17. ANTIBIOTIC RESISTANCE IN ENTEROBACTERIACEAE STRAINS ISOLATED FROM CHICKEN AND MILK SAMPLES
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Lukáš Hleba, Juraj Čuboň, Jana Petrová, Miroslava Kačániová, and Attila Kántor
- Subjects
milk ,Antibiotic resistance ,medicine.drug_class ,business.industry ,chicken ,Antibiotics ,lcsh:QR1-502 ,Drug resistance ,Biology ,Food safety ,Microbiology ,lcsh:Microbiology ,Multiple drug resistance ,Enterobacteriaceae ,Streptomycin ,Ampicillin ,medicine ,business ,Molecular Biology ,Food Science ,Biotechnology ,medicine.drug ,Food contaminant - Abstract
Antibiotic resistance and identification of strains in Enterobacteriaceae genera isolated from milk, milk products and rectal swabs of chicken was examined in this experiment. After samples collection cultivation and identification of bacterial strain was done. MALDI TOF MS Biotyper for identification of Enterobacteriaceae strains was used. For susceptibility testing disc diffusion methodology was used according by EUCAST. Results showed high level of ampicillin resistance in isolates from milk and milk samples. The highest streptomycin resistance was detected in isolates from rectal swabs of chicken. After identification, we determined that S. enterica ser. Typhimurium, which was isolated from rectal swabs of chicken showed the most multi-resistance from all identificated strains of Enterobacteriaceae. The most isolates bacterial strain was E. coli, which showed resistance against four antibiotics from rectal swabs of chicken. Also our results showed that the higher resistance level is in rectal swabs of chicken like in milk samples.
- Published
- 2015
18. MICROBIOLOGY OF RAW MATERIALS USED FOR CONFECTIONARY PRODUCTION
- Author
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Simona Kunová, Jana Petrová, Henrieta Blaškovičová, Miroslava Kačániová, Ľubomíra Juhaniaková, and Margarita Terentjeva
- Subjects
raw material products ,biology ,business.industry ,Microorganism ,digestive, oral, and skin physiology ,Microorganisms ,lcsh:QR1-502 ,filamentous microscopic fungi ,food and beverages ,Raw material ,biology.organism_classification ,Microbiology ,lcsh:Microbiology ,Coliform bacteria ,Biotechnology ,Production (economics) ,Food science ,bacteria ,Sugar ,business ,Molecular Biology ,Bacteria ,Food Science - Abstract
The aim of our study was to evaluate the microbiological quality of raw materials used for preparation of confectionery products. For microbiological evaluation total count of bacteria, mesophilic aerobic bacteria, coliform bacteria, yeast and microscopic filamentous fungi in samples of raw materials used in the manufacture and creams of confectionery products were detected. In addition to these groups of microorganisms the presence of pathogenic microorganisms Salmonella spp. and Staphylococcus aureus in creams was monitored. Products are assessed according to the limit values of the number of microorganisms defined in the Codex Alimentary of the Slovak Republic. For microbiological analysis of confectionery products, sampling of components of confectionary products and cream was carried out according to current health regulations and altogether 65 samples of components and creams were collected: 10 samples of raw materials sugar, 10 samples of flour, 10 samples of butter and 10 samples of eggs, 5 samples of butter yolk from cream-filled disposable bag without rum addition, 5 samples of butter yolk from cream-filled disposable bag with rum addition, 5 samples of cream-filled multiple use paid bag, 5 samples of cream-filled newly purchased paid bag, 5 samples of Venček corpus and 5 samples of the French cubes corpus. From raw material the highest TBC (2.65log CFU) was in flour, but the lowest in sugar (1.35 log CFU), the highest years counts was found on flour (2.42), but lowest in butter (1.18), while wasn’t in egg. In samples of creams and corpus were increased occurrence of yeast, coliform bacteria. Salmonella spp. and Staphylococcus aureus weren’t isolated from any tested sample.
- Published
- 2015
19. IMPACT OF ANISE (PIMPINELLA ANISUM) AND MINT (MENTHA PIPERITA) ESSENTIAL OILS TO MICROBIAL ACTIVITY IN CHICKEN MEAT
- Author
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Lukáš Hleba, Juraj Čuboň, Margarita Terentjeva, Jana Petrová, Peter Haščík, Simona Kunová, Henrieta Blaškovičová, Martin Mellen, and Miroslava Kačániová
- Subjects
Gram-positive bacteria ,lcsh:QR1-502 ,Vacuum packing ,Microbiology ,essential oil ,lcsh:Microbiology ,law.invention ,Anise ,chicken thigh ,law ,Antimicrobial effect ,Pimpinella anisum ,antimicrobial effect ,Food science ,Molecular Biology ,Essential oil ,biology ,business.industry ,biology.organism_classification ,Food safety ,mint ,Food packaging ,business ,Food Science ,Biotechnology ,Food contaminant - Abstract
The goal of the present study was to evaluate fresh chicken thighs quality (microbiological and sensory) after treatment by ethylenediaminetetraacetate (EDTA), Pimpinella anisum L. and Mentha piperita essential oils in 1% concentration, stored under vacuum packaging (VP), at 4±0.5°C for a period of 16 days. The following treatments of chicken thighs were applied: air-packaged (AC, control samples), vacuum-packaged (VPC, control samples), vacuum-packed with EDTA solution 1.50% v/w (VPEC, control samples), VP with Pimpinella anisum L. and Mentha piperita essential oil at concentrations 0.1% v/w (VP+AEO and VP+MEO). The quality assessment of VP product after EDTA treatment, Pimpinella anisum L. and Mentha piperita oils was done by microbiological testing and the total viable counts, Enterobacteriaceae, lactic acid bacteria and Pseudomonas aeruginosa were detected. The using of Pimpinella anisum L. and Mentha piperita oils and EDTA with combination of vacuum packaging has significant effect (P < 0.05) to reduction of microorganisms compared with control group without vacuum packaging and untreated control group.
- Published
- 2015
20. MICROORGANISMS IN SELECTED CONFECTIONARY PRODUCTS DURING THE MANUFACTURING PROCESS
- Author
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Margarita Terentjeva, Miroslava Kačániová, Ľubomíra Juhaniaková, Simona Kunová, Henrieta Blaškovičová, and Jana Petrová
- Subjects
Venček ,biology ,Bacteria ,Manufacturing process ,microscopic filamentous fungi ,Microorganism ,lcsh:QR1-502 ,yeasts ,confectionary products ,biology.organism_classification ,Microbiology ,lcsh:Microbiology ,Coliform bacteria ,Botany ,Food science ,Molecular Biology ,punch balls ,French cubes ,Food Science ,Biotechnology - Abstract
The aim of our study was to evaluate the microbiological quality confectionery products during production. A total of 135 samples were analyzed: 45 samples of the punch balls, 45 Venček samples and 45 samples French cubes from home, school and private production. For microorganism cultivation VRBL agar for the isolation of coliform bacteria, DRBC and DG18 for microscopic fungi and yeasts, Plate Count Agar for total viable count, Meat peptone agar for mesophilic aerobic bacteria, XLD agar for Salmonella sp. and Baird Parker agar for Staphylococcus aureus were used. Following microbiological parameters were tested: total viable count, mesophilic anaerobic microorganisms, coliform bacteria, yeast and microscopic filamentous fungi, Salmonella spp. and Staphylococcus aureus. Products are assessed according to the limit values of the number of microorganisms contained in the Codex Alimentary of the Slovak Republic. The overall assessment of the microbiological quality of the punch balls, we found that two samples from school factory and one sample from private producer did not meet CA SR for the total viable count. Comparing the microbiological quality of Venček with CA SR, we found that one sample of home production did not meet the requirements for this type of product. All the tested samples were Staphylococcus aureus and Salmonella spp. negative. Comparing the results of the samples with French cubes CA SR, we found that all the samples satisfy requirements.
- Published
- 2015
21. CHARACTERIZATION OF ENDOPHYTIC MICROFLORA OF ROSA CANINA FRUITS
- Author
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Margarita Terentjeva, Jana Petrová, Ladislav Bakay, Jana Černá, Miroslava Kačániová, and Katarína Rovná
- Subjects
biology ,Rosa canina ,microscopic filamentous fungi ,lcsh:QR1-502 ,yeasts ,fruits ,biology.organism_classification ,Microbiology ,lcsh:Microbiology ,microbiological properties ,Botany ,bacteria ,Molecular Biology ,Food Science ,Biotechnology - Abstract
There aren’t a lot of studies about the bacterial communities associated with the Rosa canina and the aim of this study was to characterize endophytic bacteria from fruit of Rosa canina. The fruits of R. canina, which is growing wild in Slovakia, were collected in May 2013 from four locations: Nitra-Zobor, Vrbové-Baraní dvor, Rišňovce, Modra pažiť, Slovakia. Microbiological analyses were conducted by use of standard microbiological methods by spreading of fruits homogenates onto agar plate. Total viable count and mesophilic anaerobic sporulating bacteria were determined on Plate Count Agar after incubation for 2 days at 37 °C. Pseudomonas aeruginosa enumeration was carried out after incubation of Pseudomonas Isolation agar at 48 h at 35 °C. For members of the family Enterobacteriaceae (45 °C) Violet Red Bile Glucose agar were used and incubation was carried out for 24 h at 37 °C. For determinations of fungal colonies Malt agar and Czapek-Dox agar were inoculated using the spread-plate technique and incubated at 25 °C for 5 days. The yeasts were grown in Glucose Yeast Peptone agar (aerobiosis) at 25 °C during 72 hours. The total viable count of fruits ranged from 4.07 log cfu.g-1 in Rišňovce to 4.84 log cfu.g-1 in Vrbové Baraní dvor. Number of mesophilic anaerobic sporulating bacteria ranged from 4.09 in Vrbové Baraní dvor to 4.82 log cfu.g-1 in Modrá pažiť. Number of Pseudomonas aeruginosa count ranged from 2.00 in Nitra Zobor and Vrbové Baraní dvor to 3.94 log cfu.g-1 in Modrá pažiť. In our study the number of Enterobacteriaceae genera ranged from 3.38 in Nitra Zobor to 4.25 log cfu.g-1 in Vrbové Baraní dvor. Number of yeasts ranged from 3.36 in Vrbové Baraní dvor to 3.85 log cfu.g-1 in Modrá pažiť. Number of microscopic filamentous fungi ranged from 2.60 in Modrá Pažiť to 3.52 log cfu.g-1 in Nitra Zobor. Our findings indicate that Rose plant is naturally associated with a variety of endophytic microorganisms, which have different physiological and biochemical properties.
- Published
- 2015
22. Antifungal activity of essential oils against selected terverticillate penicillia
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Elena Horská, Jana Petrová, Lukáš Hleba, Nenad Vuković, Katarína Rovná, Miroslava Kačániová, Soňa Felšöciová, Michal Stričík, and Zuzana Hajduová
- Subjects
Penicillium griseofulvum ,Antifungal Agents ,Penicillium species ,essential oils ,antifungal activity ,disc diffusion method ,Penicillium brevicompactum ,Microbial Sensitivity Tests ,lcsh:Agriculture ,chemistry.chemical_compound ,food ,Species Specificity ,Pimpinella anisum ,Botany ,Oils, Volatile ,Plant Oils ,Penicillium citrinum ,Antifungal activity ,Waste Management and Disposal ,Penicillium crustosum ,Ecology, Evolution, Behavior and Systematics ,lcsh:Environmental sciences ,lcsh:GE1-350 ,biology ,Salvia officinalis ,Penicillium ,Public Health, Environmental and Occupational Health ,lcsh:S ,Origanum ,biology.organism_classification ,food.food ,chemistry ,Penicillium expansum - Abstract
The aim of this study was to screen 15 essential oils of selected plant species, viz. [i]Lavandula angustifolia[/i], [i]Carum carvi[/i], [i]Pinus mungo var. pulmilio[/i], [i]Mentha piperita[/i], [i]Chamomilla recutita[/i] L.,[i] Pinus sylvestris[/i], [i]Satureia hortensis[/i] L., [i]Origanum vulgare[/i] L., [i]Pimpinella anisum[/i], [i]Rosmarinus officinali[/i]s L., [i]Salvia officinalis[/i] L., [i]Abietis albia etheroleum[/i], [i]Chamomilla recutita[/i] L. [i]Rausch[/i], [i]Thymus vulgaris[/i] L., [i]Origanum vulgare[/i] L. for antifungal activity against five [i]Penicillium[/i] species: [i]Penicillium brevicompactum[/i], [i]Penicillium citrinum[/i], [i]Penicillium crustosum[/i], [i]Penicillium expansum[/i] and [i]Penicillium griseofulvum[/i]. The method used for screening included the disc diffusion method. The study points out the wide spectrum of antifungal activity of essential oils against [i]Penicillium[/i] fungi. There were five essential oils of the 15 mentioned above which showed a hopeful antifungal activity: [i]Pimpinella anisum[/i], [i]Chamomilla recutita[/i] L., [i]Thymus vulgaris[/i], [i]Origanum vulgare[/i] L. The most hopeful antifungal activity and killing effect against all tested penicillia was found to be [i]Origanum vulgare[/i] L. and [i]Pimpinella anisum[/i]. The lowest level of antifungal activity was demonstrated by the oils [i]Pinus mungo var. pulmilio[/i], [i]Salvia officinalis[/i] L., [i]Abietis albia etheroleum[/i], [i]Chamomilla recutita[/i] L.[i] Rausch[/i], [i]Rosmarinus officinalis[/i].
- Published
- 2015
23. ANTIBACTERIAL ACTIVITY OF DROSERA ROTUNDIFOLIA L. AGAINST GRAM-POSITIVE AND GRAM-NEGATIVE BACTERIA
- Author
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Jana Petrová, Margarita Terentjeva, Miroslava Kačániová, Dominika Ďurechová, Lukáš Hleba, and Iwona Kata
- Subjects
Gram-negative bacteria ,Traditional medicine ,biology ,010405 organic chemistry ,Gram-positive bacteria ,biology.organism_classification ,01 natural sciences ,Microbiology ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,Drosera rotundifolia ,Drosera rotundifolia L ,Antibacterial activity ,Medicinal plants ,Molecular Biology ,Food Science ,Biotechnology ,Gram - Abstract
The medicinal use of genus Drosera, as an important antitussive for different respiratory diseases, has been known for centuries. Many of extracts from carnivorous plants exhibit various antibacterial and antifungal activities. Naphthoquinones containing extracts from Drosera have antiviral, antibacterial, antifungal, aphrodisiac, antispasmodic, antileprosy, antisclerotic and anticancer properties. The aim of the present study was to detect antibacterial activity of Drosera rotundifolia against Gram-positive and Gram-negative bacteria by the testing of MIC. For the study six strains of microorganisms were selected and there were Gram-positive bacteria -Bacillus thuringiensis (CCM 19T), Clostridium perfringens (CCM 4991), and Listeria monocytogenes (CCM 4699), as well as and Gram-negative bacteria - Escherichia coli (CCM 3988), Salmonella enterica subsp. enterica (CCM 3807) and Yersinia enterocolitica (CCM 5671). Plant extracts were isolated from three plants of Drosera rotundifolia L. (S1, S2 and S3) in different time range. The most effective extract with MIC50 value of 17.07 μg.ml-1 was S3, while forMIC90 of 19.05 μg.ml-1 were extracts S2 and S3 exhibiting antimicrobial activity against Bacillus thuringiensis, Clostridium perfringens and Listeria monocytogenes. Extracts S1, S2 showed MIC50 value 25.53 μg.ml-1for all the microorganism tested, but S3 extract revealed the same antimicrobial activity against Yersinia enterocolitica, Salmonella enterica subsp. enterica and Escherichia coli. Extract S1 has MIC90 value of 27.14 μg.ml-1 against all the microorganism tested, but S2 and S3 shared the same MIC90 for Yersinia enterocolitica, Salmonella enterica subsp. enterica and Escherichia coli.
- Published
- 2016
24. Phenolic profile and antimicrobial activities to selected microorganisms of some wild medical plant from Slovakia
- Author
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Lukáš Hleba, Elena Horská, Slobodan Sukdolak, Nenad Vuković, Jana Petrová, and Miroslava Kačániová
- Subjects
Microbiology (medical) ,Hydroxybenzoic acid ,lcsh:Arctic medicine. Tropical medicine ,biology ,Minimum inhibitory concentration ,lcsh:RC955-962 ,lcsh:R ,Chemical composition ,food and beverages ,lcsh:Medicine ,Antimicrobial activity ,Sambucus nigra ,biology.organism_classification ,Antimicrobial ,Ferulic acid ,chemistry.chemical_compound ,Infectious Diseases ,chemistry ,Botany ,Equisetum arvense ,Caffeic acid ,Food science ,Gallic acid ,Kaempferol ,Wild medical plants - Abstract
Objective To investigate the chemical composition and antimicrobial activity of the methanol extracts of Tussilago farfara (T. farfara), Equisetum arvense, Sambucus nigra (S. nigra) and Aesculus hippocastanum . Methods The antimicrobial activities of the extracts against Enterococcus raffinosus, Escherichia coli, Lactobacillus rhamnosus, Pseudomonas aeruginosa, Serratia rubidaea, Saccharomyces cerevisiae and Staphylococcus epidermis were determined by the microbroth dilution method according to Clinical and Laboratory Standards Institute, while the concentrations of main phenolic acids and flavonoids in the form of trimethylsilyl ethers were analysed using gas chromatography-mass spectrometry. The probit analysis was used for statistical evaluation. Results Of the 4 plant tested, all extracts showed a significant antimicrobial activity against one or more species of examined microorganisms. The most active antimicrobial plant extract was gathered from T. farfara , followed by Aesculus hippocastanum and Equisetum arvense . The extract from S. nigra showed no antimicrobial effects. The flavonoids quercetin and kaempferol, as well as several phenolic acids ( p -hydroxybenzoic acid, gallic acid, ferulic acid and caffeic acid) were identified in all extracts. The highest concentrations of bioactive compounds were detected in the extracts of T. farfara (9 587.6 μg/mg quercetin and 4 875.3 μg/mg caffeic acid) as well as S. nigra (4788.8 μg/mg kaempferol). Conclusions We can state that the methanolic plant extract of T. farfara showed the strongest antimicrobial activity against Saccharomyces cerevisiae as well as other tested microorganisms. At the same time, a good antimicrobial activity was found in the other medical plant extracts as well. No antimicrobial effect of the S. nigra extract was found with respect to the growth of Pseudomonas aeruginosa, Enterococcus raffinosus and Saccharomyces cerevisiae .
- Published
- 2014
25. Antibacterial activity againstClostridiumgenus and antiradical activity of the essential oils from different origin
- Author
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Lukáš Hleba, Nenad Vuković, Jana Petrová, Miroslava Kačániová, Ivan Salamon, Elena Horská, Marek Bobko, Alica Bobková, Alexander Vatľák, and Martina Fiskelová
- Subjects
DPPH ,Lavandula ,medicine.disease_cause ,Antioxidants ,chemistry.chemical_compound ,food ,Picrates ,Disk Diffusion Antimicrobial Tests ,Botany ,Oils, Volatile ,medicine ,Plant Oils ,Food science ,Clostridium ramosum ,Clostridium butyricum ,Clostridium ,Lavandula angustifolia ,biology ,Biphenyl Compounds ,Salvia officinalis ,General Medicine ,Origanum ,Clostridium perfringens ,biology.organism_classification ,Pollution ,food.food ,Anti-Bacterial Agents ,chemistry ,Food Science - Abstract
In the present study, the antimicrobial and antiradical activities of 15 essential oils were investigated. The antimicrobial activities were determined by using agar disc diffusion and broth microdilution methods against Clostridium genus and antioxidant properties of essential oils by testing their scavenging effect on DPPH radicals activities. We determined the antibacterial activity of Clostridium butyricum, Clostridium hystoliticum, Clostridium intestinale, Clostridium perfringens and Clostridium ramosum. We obtained the original commercial essential oils samples of Lavandula angustifolia, Carum carvi, Pinus montana, Mentha piperita, Foeniculum vulgare Mill., Pinus sylvestris, Satureia montana, Origanum vulgare L. (2 samples), Pimpinella anisum, Rosmarinus officinalis L., Salvia officinalis L., Abies alba Mill., Chamomilla recutita L. Rausch and Thymus vulgaris L. produced in Slovakia (Calendula a.s., Nova Lubovna, Slovakia). The results of the disk diffusion method showed very high essential oils activity against all tested strains of microorganisms. The best antimicrobial activity against C. butyricum was found at Pimpinella anisum, against C. hystoliticum was found at Pinus sylvestris, against C. intestinale was found at Satureia hortensis L., against C. perfringens was found at Origanum vulgare L. and against C. ramosum was found at Pinus sylvestris. The results of broth microdilution assay showed that none of the essential oils was active against C. hystoliticum. The best antimicrobial activity against C. butyricum was found at Abies alba Mill., against C. intestinale was found at Abies alba Mill., against C. perfringens was found at Satureia montana and against C. ramosum was found at Abius alba and Carum carvi. Antioxidant DPPH radical scavenging activity was determined at several solutions of oil samples (50 μL.mL(-1)-0.39 μL.mL(-1)) and the best scavenging effect for the highest concentration (50 μL.mL(-1)) was observed. The antioxidant properties were different in particular plant species. The highest% of inhibition after 30 min. of reaction was observed at Origanum vulgare (93%), Satureia montana (90.66%) and Lavandula augustifolia (90.22%).
- Published
- 2014
26. ANTIMICROBIAL ACTIVITY OF ROSA CANINA FLOWERS AGAINST SELECTED MICROORGANISMS
- Author
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Katarína Rovná, Miroslava Kačániová, Jana Petrová, Jana Černá, and Margarita Terentjeva
- Subjects
Gram-negative bacteria ,antimicrobial activity ,biology ,Bacteria ,flowers of Rosa canina ,Rosa canina ,microscopic filamentous fungi ,Microorganism ,fungi ,lcsh:QR1-502 ,food and beverages ,Antimicrobial ,biology.organism_classification ,Microbiology ,lcsh:Microbiology ,Botany ,Molecular Biology ,Food Science ,Biotechnology - Abstract
Rosa canina flowers were screened against various plant pathogenic microbial strains to study the antimicrobial properties of the plant. Ethanolic and methanolic extracts of flowers were screened applying agar well diffusion method against two Gram-negative bacteria including Escherichia coli CCM 3988 and Pseudomonas aeruginosa CCM 1960 and three microscopic filamentous fungi strains Aspergillus niger, Fusarium culmorum and Alternaria alternata, respectively. The best antimicrobial effect of ethanolic extract of Rosa canina flowers was found against Pseudomonas aeruginosa and the best antimicrobial effect of methanolic extract of Rosa canina flowers was found against Escherichia coli.
- Published
- 2015
27. The effects of bee pollen extracts on the broiler chicken’s gastrointestinal microflora
- Author
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Juraj Čuboň, Jana Petrová, Lukáš Hleba, Robert Chlebo, Henrieta Arpášová, Adriana Pavelková, Peter Haščík, Miroslava Kačániová, Katarína Rovná, Michal Stričík, and Alica Bobková
- Subjects
Enterococcus avium ,Colony Count, Microbial ,medicine.disease_cause ,Microbiology ,Random Allocation ,Lactobacillus acidophilus ,Enterobacteriaceae ,Pollen ,medicine ,Animals ,Food science ,General Veterinary ,biology ,food and beverages ,Klebsiella oxytoca ,Bees ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,Antimicrobial ,Proteus mirabilis ,Gastrointestinal Tract ,Lactobacillaceae ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Bee pollen ,bacteria ,Chickens - Abstract
The aim of this study was to investigate the effects of bee pollen ethanolic extracts on the in vivo gastrointestinal tract microflora colonization of broiler chickens. A completely randomized experiment based on six treatments (different concentrations of bee pollen – 0, 5, 15, 25, 35 and 45 g kg −1 diet) was used during 7 weeks. The highest count of faecal Enterococci was found in the experimental group with the addition of 15 g of pollen (8.85 ± 0.87 log CFU g −1 ) per 1 kg of feed mixture. The highest count of Lactobacilli was detected in the experimental group with 35 g of pollen per 1 kg of feed mixture and the highest number of the Enterobacteriaceae genera count was found in the control group (8.43 ± 0.15 log CFU g −1 ). Moreover, the MALDI TOF MS Biotyper identified the following genera: Escherichia coli , Proteus mirabilis , Klebsiella oxytoca , as well as Lactobacillus acidophilus , L. crispatus , L. fermentum and L. salivarius from the Lactobacilli group and Enterococcus avium , E. casseliflavus , E. cecorum , E. faecalis , E. faecium , E. gallinarum , E. hirae and E. malodoratus from the Enterococci group. Additionally, the in vitro antimicrobial activities of pollen against five bacteria species isolated from gastrointestinal tracts of chickens were tested. The best antimicrobial effect of the pollen extract was detected against K. oxytoca .
- Published
- 2013
28. ANTIMICROBIAL ACTIVITY OF NONTRADITIONAL PLANT POLLEN AGAINST DIFFERENT SPECIES OF MICROORGANISMS
- Author
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Maciej Kluz, Jana Petrová, Nenad Vuković, Tetiana Shevtsova, Lukáš Hleba, Miroslava Kačániová, Margarita Terentjeva, and Przemyslaw Rožek
- Subjects
antimicrobial activity ,microscopic filamentous fungi ,Microorganism ,fungi ,lcsh:QR1-502 ,food and beverages ,Pathogenic bacteria ,pathogenic bacteria ,Biology ,medicine.disease_cause ,Antimicrobial ,Aspergillosis ,medicine.disease ,Microbiology ,lcsh:Microbiology ,Pollen ,Botany ,medicine ,Molecular Biology ,Nontraditional plant pollen ,Food Science ,Biotechnology - Abstract
The aim of this study was to detect the antimicrobial activity of four plant pollen samples to pathogenic bacteria, microscopic fungi and yeasts. Pollens of dogwood common (Cornus mas), ray mountain (Secale strictum spp. strictum), pumpkin rape (Cucurbita pepo var. styriaca) and grape vine (Vitis vinifera) were collected in 2010 in Slovakia. The antimicrobial effects of the four nontraditional plant pollens were tested using the agar well diffusion method. For extraction, 70% ethanol (aqueous, v/v) was applied. Antimicrobial susceptibility of five different strains of bacteria - three gram positive (Listeria monocytogenes CCM 4699, Pseudomonas aeruginosa CCM 1960, Staphylococcus aureus CCM 3953) and gram negative (Salmonella enterica CCM 4420, Escherichia coli CCM 3988), as well as three different strains of microscopic fungi, Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger, and three different strains of yeasts Candida albicans, Geotrichum candidum and Rhodotorula mucilaginosa, were examinated. L. monocytogenes was the most sensitive among bacteria to the three ethanol extracts of plant pollen after 24 hours of inoculation, A. flavus and C. albicans were the most sensitive microscopic fungi and yeast species, respectively.
- Published
- 2014
29. The effect of vacuum packaging, EDTA, oregano and thyme oils on the microbiological quality of chicken's breast
- Author
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Katarína Rovná, Jana Petrová, Lukáš Hleba, Elena Horská, Adriana Pavelková, and Miroslava Kačániová
- Subjects
Preservative ,food.ingredient ,Meat ,Vacuum ,Food spoilage ,Vacuum packing ,Biology ,Plate count agar ,Shelf life ,Microbiology ,Thymus Plant ,chemistry.chemical_compound ,food ,Refrigeration ,Origanum ,Oils, Volatile ,Agar ,Animals ,Food science ,Edetic Acid ,Biological Products ,Food Packaging ,biology.organism_classification ,Bacterial Load ,Food packaging ,Lactobacillus ,Infectious Diseases ,chemistry ,Consumer Product Safety ,Pseudomonas aeruginosa ,Food Preservatives ,Chickens - Abstract
The effect of ethylenediaminetetraacetate (EDTA), oregano (Origanum vulgare) and thyme (Thymus vulgaris) oils, on the chicken breast fillets was examined in this study. The chicken breast fillets were stored under vacuum packaging (VP), at 4 ± 0.5 °C for a period of 18 days. There were used the following treatments of chicken breast fillets: Air-packaged (AC, control samples), vacuum-packaged (VPC, control samples), VP with EDTA solution 1.50% w/w (VPEC, control samples), VP with oregano oil 0.20% v/w (VP + O) and VP with thyme oil 0.20% v/w, (VP + T). The quality assessment for vacuum packaging of the product in accordance with the terms above and EDTA treatment, oregano and thyme oil was established by microbiological analyzes. The microbiological properties as the total viable counts on Plate Count Agar, after incubation for 2 days at 37 °C and coliform bacteria on Violet Red Bile Glucose agar incubated at 37 °C for 24 h, lactobacilli on Rogosa and Sharpe agar after incubation 48-78 h at 37 °C in an aerobic atmosphere supplemented with carbon dioxide (5% CO2) and Pseudomonas aeruginosa on Pseudomonas Isolation agar (PIA, Oxoid, UK) after incubation at 48 h at 35 °C were monitored. The using of oregano, thyme oil and EDTA with combination of vacuum packaging has significant effects to reduction of all followed groups of microorganisms compared with control group without vacuum packaging and untreated control group. The natural preservatives can be used as alternatives to chemical additives which could extend the meat and meat products shelf life. The knowledge about them can have an important economic feedback by reducing losses attributed to spoilage and by allowing the products to reach distant and new markets. This study shows how using of natural antimicrobials can extend the shelf-life of the meat product.
- Published
- 2013
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