1. SERS Imaging of Cell-Surface Biomolecules Metabolically Labeled with Bioorthogonal Raman Reporters.
- Author
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Xiao, Ming, Lin, Liang, Li, Zefan, Liu, Jie, Hong, Senlian, Li, Yaya, Zheng, Meiling, Duan, Xuanming, and Chen, Xing
- Subjects
BIOMOLECULES ,RAMAN scattering ,ALKYNES ,AZIDES ,NITROGEN compounds - Abstract
Live imaging of biomolecules with high specificity and sensitivity as well as minimal perturbation is essential for studying cellular processes. Here, we report the development of a bioorthogonal surface-enhanced Raman scattering (SERS) imaging approach that exploits small Raman reporters for visualizing cell-surface biomolecules. The cells were cultured and imaged by SERS microscopy on arrays of Raman-enhancing nanoparticles coated on silicon wafers or glass slides. The Raman reporters including azides, alkynes, and carbondeuterium bonds are small in size and spectroscopically bioorthogonal (background-free). We demonstrated that various cell-surface biomolecules including proteins, glycans, and lipids were metabolically incorporated with the corresponding precursors bearing a Raman reporter and visualized by SERS microscopy. The coupling of SERS microscopy with bioorthogonal Raman reporters expands the capabilities of live-cell microscopy beyond the modalities of fluorescence and label-free imaging. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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