1. Human Umbilical Cord Wharton’s Jelly Derived Mesenchymal Stromal Cells May Attenuate Sarcopenia in Aged Mice Induced by Hindlimb Suspension
- Author
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Yan-Zhen Bi, Yuzhong Wang, Jing Hu, Quan-Quan Wang, Yan-Xin Li, Xiao-Ma Jing, Yanlei Hao, Yun Chen, Baojun Qiao, and Xiao-Fu Cao
- Subjects
0301 basic medicine ,Male ,medicine.medical_specialty ,Sarcopenia ,Apoptosis ,Mesenchymal Stem Cell Transplantation ,Umbilical cord ,Umbilical Cord ,03 medical and health sciences ,Gastrocnemius muscle ,Mice ,Internal medicine ,Wharton's jelly ,medicine ,Myocyte ,Animals ,Humans ,Wharton Jelly ,Cell Proliferation ,Inflammation ,Mesenchymal Stromal Cells ,business.industry ,Animal Study ,Mesenchymal stem cell ,Skeletal muscle ,Cell Differentiation ,Mesenchymal Stem Cells ,General Medicine ,Hindlimb Suspension ,medicine.disease ,Mice, Inbred C57BL ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,business - Abstract
BACKGROUND Since the use of human umbilical cord Wharton's Jelly derived mesenchymal stromal cells (hWJ-MSCs) to treat sarcopenia has not been explored, we studied the effects of hWJ-MSCs in aged male C57BL/6J mice with sarcopenia induced by hindlimb suspension, and explored the potential mechanism. MATERIAL AND METHODS Hindlimb suspension was used to induce sarcopenia in 24-month-old C57BL/6J mice and green fluorescent protein-tagged hWJ-MSCs and controls were transplanted into mice via tail vein or local intramuscular injection. After hWJ-MSC transplantation, changes in whole body muscle strength and endurance, gastrocnemius muscle weight and myofiber cross-sectional area (CSA) were studied. Proliferation of skeletal muscle stem cell, apoptosis, and chronic inflammation were also investigated. RESULTS We demonstrated that whole body muscle strength and endurance, gastrocnemius muscle mass, and CSA were significantly increased in hWJ-MSC-transplanted mice than in controls (P
- Published
- 2018