Search

Owing to the threat of cadmium (Cd 2+ ) to public health, it is an urgent demand to develop effective, sensitive, and rapid methods for the detection of cadmium. In this study, indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and immunochromatographic test strips (ICTS) were established for the determination of Cd 2+ based on the obtained mAb with high specificity and high affinity (Kaff = 3.0 × 10 9 L/moL). The linear range of ic-ELISA detection was 0.03-1.11 ng/mL and 50% inhibitive concentration (IC50) of cadmium ion was determined to be 0.15 ng/mL. The visual limit of detection (vLOD) of the AuNS-based strip was 0.375 ng/mL. The vLOD of AuNF-based strip using higher intensity reporter determined to be 0.03 ng/mL, which was enhanced 12 times compared to the traditional strip. In summary, the developed immunoassays based on mAb shows great potential for monitoring the cadmium ion in environmental samples., (Copyright © 2021 Elsevier B.V. All rights reserved.)

The stress effect of cadmium absorption between aloe and Brassica junica was studied through a simulation experiment with different cadmium contents in soil. The results showed that the growth characteristics, biomass and cadmium content of aloe and B. junica plants were related to pH and cadmium content in the soil. When aloe was intercropped with B. junica, soil pH was higher than that of single planting, showing that intercropping aloe and B. junica can reduce aloe's absorption of Cd. When Cd content in the soil was high, the absorption coefficient of aloe was lower than that of B. junica, and Cd content in the leaves of intercropped aloe was 15.8%-79.2% lower than that of aloe cropped only. In addition, planting B. junica in the soil with different contents of Cd had certain stress effect on aloe's absorption of Cd, thereby protecting aloe well, improving safety of aloe planting and showing its potential tor application in phytoremediation. [ABSTRACT FROM AUTHOR]

The stress effect of cadmium absorption between aloe and Solarium nigrum L was studied through a simulation experiment with different cadmium contents in soil. The results showed that the growth characteristics, biomass and Cd content in the plants of aloe and S. nigrum L were closely related to Cd content in the soil, and S. nigrum L and aloe had a strong tolerance to Cd in the experiment. pH of rhizosphere soil with S. nigrum L and aloe planted was generally higher than that of non-rhizosphere soil, which is related to the planting pattern. Compared with aloe, S. nigrum L had a stronger capacity to accumulate Cd, and intercropping S. nigrum L and aloe could inhibit aloe's absorption of Cd, which is favorable to the sate planting of aloe. In addition, S. nigrum L has the potential to restore polluted soil. [ABSTRACT FROM AUTHOR]