1. Direct electron transfer and electrocatalysis of hemoglobin adsorbed on mesoporous carbon through layer-by-layer assembly
- Author
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Feng, Jiu-Ju, Xu, Jing-Juan, and Chen, Hong-Yuan
- Subjects
- *
CARBON electrodes , *HEMOGLOBINS , *CHARGE exchange , *ELECTROCATALYSIS - Abstract
Abstract: Using chitosan as an effective linker between CMK-3 and glassy carbon electrode surface, {Hb/CMK-3} n multilayer film-modified electrodes were constructed through layer-by-layer assembly. The morphology of thus-formed {Hb/CMK-3} n film was characterized by scanning electron micrographs, and the interaction of hemoglobin (Hb) with CMK-3 was studied by UV–vis spectroscopy and electrochemical methods. Under optimal conditions, {Hb/CMK-3}6 film showed a couple of stable and well-defined redox peaks at about −377 and −296mV in pH 7.0 buffers. Furthermore, the {Hb/CMK-3}6 film displayed excellent electrocatalysis to the reduction of both H2O2 and O2. Based on thus-formed film and its direct electron transfer behavior, a novel biosensor was presented for the determination of H2O2 ranging from 1.2 to 57μM with the detection limit of 0.6μM at S/N=3. CMK-3 provided a desirable matrix for protein immobilization and biosensor preparation. [Copyright &y& Elsevier]
- Published
- 2007
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