1. Inhibition on Numb/Notch signal pathway enhances radiosensitivity of lung cancer cell line H358.
- Author
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Song SG, Yu HY, Ma YW, Zhang F, and Xu XY
- Subjects
- Animals, Apoptosis radiation effects, Biomarkers, Tumor genetics, Biomarkers, Tumor metabolism, Blotting, Western, Carcinoma, Non-Small-Cell Lung metabolism, Carcinoma, Non-Small-Cell Lung pathology, Cell Movement radiation effects, Cell Proliferation radiation effects, Gene Expression Regulation, Neoplastic radiation effects, Humans, Lung Neoplasms metabolism, Lung Neoplasms pathology, Membrane Proteins genetics, Membrane Proteins metabolism, Mice, Mice, Inbred BALB C, Mice, Nude, Nerve Tissue Proteins genetics, Nerve Tissue Proteins metabolism, Radiation, Ionizing, Real-Time Polymerase Chain Reaction, Receptors, Notch genetics, Receptors, Notch metabolism, Tumor Cells, Cultured, Xenograft Model Antitumor Assays, Carcinoma, Non-Small-Cell Lung radiotherapy, Lung Neoplasms radiotherapy, Membrane Proteins antagonists & inhibitors, Nerve Tissue Proteins antagonists & inhibitors, Radiation Tolerance, Receptors, Notch antagonists & inhibitors, Signal Transduction radiation effects
- Abstract
The objective of the study is to investigate the effects of the Numb/Notch signal pathway on the radiosensitivity of lung cancer cell line H358. MTT assay and colony forming assay were used to detect the effects of different doses of X-rays and MW167 on the in vitro proliferation of the lung cancer cell line H358. Flow cytometry was applied to evaluate the effects of X rays on the apoptosis of H358. Scratch assay and Transwell invasion assay were used to examine the effects of X-rays on the migration and invasion abilities of H358. The mRNA and protein expressions in the signal pathway were detected by real-time PCR and western blot. Assays in vitro confirmed the effects of the Numb/Notch pathway inhibitor on the radiosensitivity to lung cancer. MW167 enhanced the inhibiting effects of X-ray on the proliferation of H358 cell line. After the addition of MW167, the apoptosis rates significantly increased, but the invasion and migration abilities decreased significantly. Meanwhile, MW167 could dose-dependently promote the increase of expression of Numb, which is the upstream gene of the Numb/Notch signaling pathway, but inhibit the expression of and HES1. In vivo experiments revealed that cell proliferation was suppressed in the radiation, pathway inhibitor, and pathway inhibitor + radiation groups, and the pathway inhibitor + radiation group exhibited more active anti-tumor ability when compared with the blank group (all P < 0.05); Numb expression was up-regulated, but Notch1 and HES1 expressions were down-regulated in those three groups, and also, the pathway inhibitor + radiation group exhibited more significant alternation when compared with the blank group (all P < 0.05); cell apoptosis was promoted in those three groups, and the pathway inhibitor + radiation group showed more active apoptosis when compared with the blank group (all P < 0.05). Repression of the Numb/Notch pathway enhances the effects of radiotherapy on the radiosensitivity of the lung cancer cell line H358, and thus the Numb/Notch pathway may be a new target of radiotherapy for lung cancer.
- Published
- 2016
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