3 results on '"Wang, Bingyun"'
Search Results
2. Manufacturing and banking canine adipose-derived mesenchymal stem cells for veterinary clinical application
- Author
-
Wang Bingyun, Huina Luo, Li Dongsheng, Chen Shengfeng, and Chen Zhisheng
- Subjects
Male ,Quality Control ,Veterinary medicine ,Sterility ,Carcinogenicity Tests ,Adipose ,Cell Culture Techniques ,Adipose tissue ,Cell Separation ,Mice, SCID ,Tissue Banks ,Mesenchymal Stem Cell Transplantation ,Cryopreservation ,Cell therapy ,Canine ,Parvoviridae Infections ,Parvovirus ,03 medical and health sciences ,0302 clinical medicine ,Dogs ,In vivo ,Medicine ,Animals ,Cell isolation ,Bioprocess ,030304 developmental biology ,0303 health sciences ,lcsh:Veterinary medicine ,General Veterinary ,business.industry ,Mesenchymal stem cell ,General Medicine ,Leukopenia ,Banking ,Adipose Tissue ,030220 oncology & carcinogenesis ,Mesenchymal stem cells ,lcsh:SF600-1100 ,Female ,business ,Research Article - Abstract
Background Mesenchymal stem cells (MSCs) have generated a great amount of interest in recent years as a novel therapeutic application for improving the quality of pet life and helping them free from painful conditions and diseases. It has now become critical to address the challenges related to the safety and efficacy of MSCs expanded in vitro. In this study, we establish a standardized process for manufacture of canine adipose-derived MSCs (AD-MSCs), including tissue sourcing, cell isolation and culture, cryopreservation, thawing and expansion, quality control and testing, and evaluate the safety and efficacy of those cells for clinical applications. Results After expansion, the viability of AD-MSCs manufactured under our standardized process was above 90 %. Expression of surface markers and differentiation potential was consistent with ISCT standards. Sterility, mycoplasma, and endotoxin tests were consistently negative. AD-MSCs presented normal karyotype, and did not form in vivo tumors. No adverse events were noted in the case treated with intravenously AD-MSCs. Conclusions Herein we demonstrated the establishment of a feasible bioprocess for manufacturing and banking canine AD-MSCs for veterinary clinical use.
- Published
- 2021
3. A Comparative Study of Biological Characteristics and Transcriptome Profiles of Mesenchymal Stem Cells from Different Canine Tissues
- Author
-
Chen Shengfeng, Can-Ying Liu, Huina Luo, Bai Yinshan, Saeed El-Ashram, Wang Bingyun, Chen Zhisheng, Luo Dongzhang, Ji Huiqin, and Zhan Xiaoshu
- Subjects
0301 basic medicine ,040301 veterinary sciences ,RNA-sequencing ,CD34 ,canine ,Adipose tissue ,Article ,Catalysis ,lcsh:Chemistry ,0403 veterinary science ,Inorganic Chemistry ,Cell therapy ,Transcriptome ,03 medical and health sciences ,Dogs ,biological characteristics ,Cell Adhesion ,medicine ,Animals ,Cluster Analysis ,Physical and Theoretical Chemistry ,lcsh:QH301-705.5 ,Molecular Biology ,Cells, Cultured ,Spectroscopy ,Cell Proliferation ,mesenchymal stem cells ,biology ,Organic Chemistry ,CD44 ,Mesenchymal stem cell ,Reproducibility of Results ,Cell Differentiation ,04 agricultural and veterinary sciences ,General Medicine ,Computer Science Applications ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,lcsh:Biology (General) ,lcsh:QD1-999 ,Gene Expression Regulation ,Organ Specificity ,Adipogenesis ,biology.protein ,Bone marrow ,Biomarkers - Abstract
Mesenchymal stem cells (MSCs) are the most promising seed cells for cell therapy. Comparing the biological and transcriptome gene characteristics of MSCs from different sources provides an important basis for the screening of clinically used cells. The main purpose of this experiment was to establish methods for the isolation and culture of MSCs from five different canine sources, including adipose tissue, bone marrow, umbilical cord, amniotic membrane, and placenta, and compare biological and transcriptome characteristics of MSCs, in order to provide a basis for the clinical application of canine MSCs. MSCs were isolated from Chinese pastoral dogs, and the following experiments were performed: (1) the third, sixth, and ninth generations of cells were counted, respectively, and a growth curve was plotted to calculate the MSC population doubling time, (2) the expression of CD34 and CD44 surface markers was studied by immunofluorescence, (3) the third generation of cells were used for osteogenetic and adipogenic differentiation experiments, and (4) MSC transcriptome profiles were performed using RNA sequencing. All of the five types of MSCs showed fibroblast-like adherent growth. The cell surface expressed CD44 instead of CD34, the third-generation MSCs had the highest proliferative activity. The average population doubling time of adipose mesenchymal stem cells (AD-MSCs), placenta mesenchymal stem cells (P-MSCs), bone marrow mesenchymal stem cells (BM-MSCs), umbilical cord mesenchymal stem cells (UC-MSCs), and amniotic mesenchymal stem cells (AM-MSCs) were 15.8 h, 21.2 h, 26.2 h, 35 h, and 41.9 h, respectively. All five types of MSCs could be induced to differentiate into adipocytes and osteoblasts in vitro, with lipid droplets appearing after 8 days and bone formation occurring 5 days after AD-MSC induction. However, the multilineage differentiation for the remaining of MSCs was longer compared to that of the AD-MSCs. The MSC transcriptome profiles showed that AD-MSC and BM-MSCs had the highest homology, while P-MSCs were significantly different compared to the other four types of MSCs. All the isolated MSCs had the main biological characteristics of MSCs. AD-MSCs had the shortest time for proliferation, adipogenesis, and osteogenic differentiation.
- Published
- 2019
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.