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1. Greatly reduced amino acid alphabets in directed evolution: making the right choice for saturation mutagenesis at homologous enzyme positions.

Author

Manfred T. Reetz and Sheng Wu

Subjects
*AMINO acids, *MUTAGENESIS, *RADIOGENETICS, *ENANTIOSELECTIVE catalysis, *MONOOXYGENASES, *CHEMICAL reactions
Abstract

Enantioselective mutants of the thermally robust phenyl acetone monooxygenase (PAMO) as catalysts in Baeyer–Villiger reactions have been evolved by utilizing saturation mutagenesis in which drastically reduced amino acid alphabets are employed at homologous enzyme positions. [ABSTRACT FROM AUTHOR]

Published
2008
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