179 results on '"Cui An"'
Search Results
2. Cation−π Interaction Induced Excimer Formation: A New Strategy for High‐Efficiency Organic Solid‐State Luminescence.
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Cui, Xingyu, Hao, Yuxia, Guan, Weijiang, Liu, Lihong, Shi, Wenying, and Lu, Chao
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EXCIMERS , *SCIENTISTS , *LUMINESCENCE , *CHEMISTRY , *ACIDIFICATION , *BENZENE , *MOLECULES - Abstract
Organic solid‐state luminescence materials have shown great promise in many forefront areas of modern chemistry. However, the well‐developed organic luminescent solids usually provoke a mass of synthetic steps. To better utilize their performances, the development of simple strategies for host materials is a goal of general concern. Herein, a series of highly efficient solid‐state luminescence materials are attained with aid of one‐step acidification of commercially available molecules. The mechanism demonstrates that the luminous efficiency of the solid‐state molecules is efficiently improved due to the synergy from the emergence of cation–π interaction and the formation of excimer. The cation–π interaction replaces π–π packing, leading to the formation of dimers with higher rigidity in solid state accompanied by red‐shifted emissions. Therefore, this one‐step acidification design concept will light the great passion of scientists for the fabrication of cation−π‐triggered analogous organic solid‐state luminescence materials with different colors by changing the substituents on benzene moieties. [ABSTRACT FROM AUTHOR]
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- 2020
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3. Sedimentology and chemostratigraphy of the terminal Ediacaran Dengying Formation at the Gaojiashan section, South China.
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Cui, Huan, Xiao, Shuhai, Cai, Yaoping, Peek, Sara, Plummer, Rebecca E., and Kaufman, Alan J.
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CHEMOSTRATIGRAPHY , *SEDIMENTOLOGY , *EDIACARAN fossils , *FOSSIL animals , *ANALYTICAL geochemistry , *SEDIMENTATION & deposition , *CHEMISTRY , *BRECCIA - Abstract
The terminal Ediacaran Dengying Formation (c. 551.1–538.8 Ma) in South China is one of two successions where Ediacara-type macrofossils are preserved in carbonate facies along with skeletal fossils and bilaterian animal traces. Given the remarkable thickness of carbonate-bearing strata deposited in less than 12.3 million years, the Dengying Formation holds the potential for construction of a relatively continuous chemostratigraphic profile for the terminal Ediacaran Period. In this study, a detailed sedimentological and chemostratigraphic (δ13Ccarb, δ18Ocarb, δ13Corg, δ34Spyrite, and 87Sr/86Sr) investigation was conducted on the Dengying Formation at the Gaojiashan section, Ningqiang County of southern Shaanxi Province, South China. Sedimentological results reveal an overall shallow-marine depositional environment. Carbonate breccia, void-filling botryoidal precipitates and aragonite crystal fans are common in the Algal Dolomite Member of the Dengying Formation, suggesting that peritidal facies were repeatedly karstified. The timing of karstification was likely early, probably soon after the deposition of the dolomite sediments. The presence of authigenic aragonite cements suggests high alkalinity in the terminal Ediacaran ocean. Geochemical analysis of micro-drilled samples shows that distinct compositions are registered in different carbonate phases, which should be considered when constructing chemostratigraphic profiles representative of true temporal variations in seawater chemistry. Integrated chemostratigraphic data suggest enhanced burial of organic carbon and pyrite, and the occurrence of extensive marine anoxia (at least in the Gaojiashan Member). Rapid basinal subsidence and carbonate accumulation during a time of elevated seawater alkalinity and increased rates of pyrite burial may have facilitated the evolutionary innovation of early biomineralizing metazoans. [ABSTRACT FROM AUTHOR]
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- 2019
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4. Protective approaches and mechanisms of microencapsulation to the survival of probiotic bacteria during processing, storage and gastrointestinal digestion: A review.
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Liu, Huan, Cui, Steve W., Chen, Maoshen, li, Yue, Liang, Rong, Xu, Feifei, and Zhong, Fang
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MICROENCAPSULATION , *PROBIOTICS , *DIGESTION , *SPRAY drying , *BACTERIA , *HIGH temperatures , *STORAGE , *LOW temperatures , *CHEMISTRY , *FREEZE-drying - Abstract
In recent years, there is a rising interest in the number of food products containing probiotic bacteria with favorable health benefit effects. However, the viability of probiotic bacteria is always questionable when they exposure to the harsh environment during processing, storage, and gastrointestinal digestion. To overcome these problems, microencapsulation of cells is currently receiving considerable attention and has obtained valuable effects. According to the drying temperature, the commonly used technologies can be divided into two patterns: high temperature drying (spray drying and fluid bed drying) and low temperature drying (ultrasonic vacuum spray drying, spray chilling, electrospinning, supercritical technique, freeze drying, extrusion, emulsion, enzyme gelation, and impinging aerosol technique). Furthermore, not only should the probiotic bacteria maintain high viability during processing but they also need to keep alive during storage and gastrointestinal digestion, where they additionally suffer from water, oxygen, heat as well as strong acid and bile conditions. This review focuses on demonstrating the effects of different microencapsulation techniques on the survival of bacteria during processing as well as protective approaches and mechanisms to the encapsulated probiotic bacteria during storage and gastrointestinal digestion that currently reported in the literature. [ABSTRACT FROM AUTHOR]
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- 2019
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5. The prevalence and persistence of aberrant promoter DNA methylation in benzene-exposed Chinese workers.
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Ren, Jingchao, Cui, Jun-peng, Luo, Mengkai, Liu, Huan, Hao, Pengfei, Wang, Xiao, and Zhang, Guang-hui
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DNA methylation , *DNA mismatch repair , *POLLUTANTS , *DNA repair , *ORGANIC chemistry - Abstract
Aberrant DNA methylation patterns are common in cancers and environmental pollutant exposed subjects. Up to date, few studies have examined the aberrant DNA methylation patterns in benzene exposed workers. We recruited 141 benzene-exposed workers, including 83 benzene-exposed workers from a shoe factory in Wenzhou and 58 workers from a painting workshop in Wuhu, 35 workers in Wuhu were followed from 2009 to 2013, and 48 indoor workers as controls from Wenzhou. We used high-resolution melting (HRM) to quantitate human samples of DNA methylation in long interspersed nuclear element-1 (LINE-1), (6)-methylguanine-DNA methyltransferase (MGMT), and DNA mismatch repair gene human mutator L homologue 1 (hMLH1). AML-5 cells were treated with benzoquinone (BQ) and hydroquinone (HQ), and the promoter methylation of MGMT and hMLH1 was detected using the bisulfite sequencing PCR method. The degree of LINE-1 methylation in benzene-exposed workers was significantly lower than that of the controls (p<0.001), and the degree of MGMT (p<0.001) and hMLH1 (p = 0.01) methylation was significantly higher than that of the controls. The in vitro study validated the aberrant hypermethylation of hMLH1 after treatment with BQ. Among the cohort workers who were followed from 2009 to 2013, the LINE1 methylation elevated in 2013 than 2009 (p = 0.004), and premotor methylation in hMLH1 reduced in 2013 than 2009 (p = 0.045) with the reduction of the benzene exposure. This study provides evidence that benzene exposure can induce LINE-1 hypomethylation and DNA repair gene hypermethylation. [ABSTRACT FROM AUTHOR]
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- 2019
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6. Stability and Biological Activity Evaluation of Chlorantraniliprole Solid Nanodispersions Prepared by High Pressure Homogenization.
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Cui, Bo, Feng, Lei, Wang, Chunxin, Yang, Dongsheng, Yu, Manli, Zeng, Zhanghua, Wang, Yan, Sun, Changjiao, Zhao, Xiang, and Cui, Haixin
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CHLORANTRANILIPROLE , *PESTICIDE analysis , *AGRICULTURAL productivity , *FREEZE-drying , *PARTICLE size determination , *POLLUTION - Abstract
Poorly water-soluble compounds are difficult to develop as pesticide products and face great challenges in water-based and environmentally friendly formulation development. In this study, high pressure homogenization combined with lyophilization was adopted to prepare the solid nanodispersions of chlorantraniliprole with poor solubility and high melting point. The mean particle sizes of the solid nanodispersions with different pesticide contents were all less than 75 nm, even when the content was up to 91.5%. For the 2.5% chlorantraniliprole solid nanodispersion with the mean particle size of 29 nm, the suspensibility and wetting time in water were 97.32% and 13 s, respectively. The re-dispersibility and wettability were superior to those of conventional water dispersible granules. The retention on the rice leaf of 18.7 mg/cm2 was 1.5 and 3 times that of commercial aqueous suspension concentrate and pure water. The bioassay result to diamondback moths indicated that the toxicity of the solid nanodispersion was 3.3 and 2.8 times that of technical and aqueous suspension concentrate, respectively. Moreover, the solid nanodispersion has the advantages of total avoidance of organic solvents, significant reduction of surfactants and feasibility of obtaining high concentration nanoformulations. The solid nanodispersion is an attractive candidate for improving pesticide solubility and efficacy, and its application in crop production will reduce both residues in food and environmental pollution of pesticide. [ABSTRACT FROM AUTHOR]
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- 2016
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7. Host-dependent nitrogen recycling as a mechanism of symbiont control in Aiptasia.
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Cui, Guoxin, Liew, Yi Jin, Li, Yong, Kharbatia, Najeh, Zahran, Noura I., Emwas, Abdul-Hamid, Eguiluz, Victor M., and Aranda, Manuel
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SYMBIOSIS , *AMINO acid synthesis , *AMINO acid metabolism , *PHYSICAL sciences , *NITROGEN , *CARBOHYDRATE metabolism - Abstract
The metabolic symbiosis with photosynthetic algae allows corals to thrive in the oligotrophic environments of tropical seas. Different aspects of this relationship have been investigated using the emerging model organism Aiptasia. However, many fundamental questions, such as the nature of the symbiotic relationship and the interactions of nutrients between the partners remain highly debated. Using a meta-analysis approach, we identified a core set of 731 high-confidence symbiosis-associated genes that revealed host-dependent recycling of waste ammonium and amino acid synthesis as central processes in this relationship. Subsequent validation via metabolomic analyses confirmed that symbiont-derived carbon enables host recycling of ammonium into nonessential amino acids. We propose that this provides a regulatory mechanism to control symbiont growth through a carbon-dependent negative feedback of nitrogen availability to the symbiont. The dependence of this mechanism on symbiont-derived carbon highlights the susceptibility of this symbiosis to changes in carbon translocation, as imposed by environmental stress. [ABSTRACT FROM AUTHOR]
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- 2019
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8. Microbial origin of bioflocculation components within a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China.
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Mu, Jun, Cui, Xia, Shao, Mingjiao, Wang, Yuxia, Yang, Qiao, Yang, Guangfeng, and Zheng, Liying
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FLOCCULANTS , *MANILA clam , *NATURAL resources , *AQUACULTURE , *MUD , *PHYSICAL sciences , *EARTH sciences - Abstract
Ruditapes philippinarum conglutination mud (RPM) is a byproduct from the aquiculture of an important commercially bivalve mollusk R. philippinarum and has been recently reported as a promising natural bioflocculant resource. However the origin of bioflocculation components within RPM is still a pending doubt and impedes its effective exploitation. This study investigated the probability that RPM bioflocculation components originate from its associated microbes. RPM samples from an aquaculture farm in Zhoushan of China were applied to characterize its microbial community structure, screen associated bioflocculant-producing strains, and explore the homology between extracellular polysaccharides (EPS) from bioflocculant-producing isolates and RPM flocculation components. Results showed that RPM exhibited high bacterial biodiversity, with Proteobacteria, Bacteroidetes and Actinobacteria as the most abundant phyla; hgcI_clade, CL500_29_marine_group, Fusibacter, MWH_UniP1_aquatic_group and Arcobacter as the dominant genera. Fourteen highly efficient bioflocculant-producing strains were screened and phylogenetically identified as Pseudoalteromonas sp. (5), Psychrobacter sp. (3), Halomonas sp. (2), Albirhodobacter sp. (1), Celeribacter sp. (1), Kocuria sp. (1) and Bacillus sp. (1), all of which except Bacillus sp. were reported for the first time for their excellent flocculation capability. Furthermore, EPS from the bioflocculant-producing strains exhibited highly similar monosaccharide composition to the reported flocculation-effective RPM polysaccharides. On the other hand, the existence of fungi in RPM was rare and showed no flocculation functionality. Findings from Zhoushan RPM strongly supported that RPM flocculation components were of bacterial origin and make RPM reproduction possible by fermentation approach. [ABSTRACT FROM AUTHOR]
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- 2019
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9. Metal-organic framework-based heterogeneous catalysts for the conversion of C1 chemistry: CO, CO2 and CH4.
- Author
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Cui, Wen-Gang, Zhang, Guo-Ying, Hu, Tong-Liang, and Bu, Xian-He
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HETEROGENEOUS catalysts , *CHEMISTRY , *POROUS metals - Abstract
Graphical abstract This review firstly summarized recent research progresses on the syntheses and applications of MOF-based heterogeneous catalysts for the conversion of a selection of C1 compounds, namely CO, CO 2 and CH 4 , into value-added chemicals. Highlights • MOF-based heterogeneous catalysts for C1 chemistry are systematically reviewed. • The MOFs, MOF composites and MOF derivatives catalysts are rationally summarized. • Major challenges and perspectives toward the further exploration are presented. Abstract Catalysis of C1 chemistry, especially for carbon monoxide (CO), carbon dioxide (CO 2) and methane (CH 4), is critically important for the clean production of fuels and chemicals and future energy sustainability. However, due to the relatively inert nature and low reactivity of these C1 molecules, their activation and transformation into clean fuels and high value-added chemicals still remain formidable challenges. In this context, metal-organic frameworks (MOFs), as relatively new emerging crystalline porous materials, have been shown to be promising heterogeneous catalysts or supports/precursors in the design and synthesis of various functional nanomaterials for addressing these challenges. In this review, the recent advances in MOF-based heterogeneous catalysts for transforming CO, CO 2 and CH 4 into high value-added chemicals are systematically reviewed. Emphasis is mainly placed on the catalytic reactivity, reaction mechanism and catalyst design. Additionally, major challenges and opportunities for MOF catalysts in the conversion of C1 chemistry are discussed to outline aspects for further development in this ongoing research field. It is anticipated that this review will provide a useful guide to chemists and material scientists attempting to design better MOF-based catalysts for the chemical conversion of C1 compounds. [ABSTRACT FROM AUTHOR]
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- 2019
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10. Goal-directed design of metal–organic frameworks for liquid-phase adsorption and separation.
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Zhang, Qi, Cui, Yuanjing, and Qian, Guodong
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METAL organic chemical vapor deposition , *METAL ions , *LIQUID phase epitaxy , *CHEMISTRY , *MOLECULAR recognition - Abstract
Graphical abstract Highlights • MOFs and derived membranes for liquid-phase adsorption/separation are reviewed. • Goal-directed design methodologies of host–guest interactions are summarized. • Molecular recognition needs fine tuning of different host–guest interactions. • Advantage and deficiency of current task-specific design are included. Abstract Metal–organic frameworks (MOFs), which are constructed from metal ions/clusters with organic linkers, are in a stage from fundamentals to applications. The application of MOFs in liquid-phase adsorption/separation is one of the most significant headways in the environment and chemistry. Concerning tunable porosity, multiple functional sites and devisable host–guest interactions, goal-directed designs lead to task-specific MOFs and are requisite to perform precise molecular recognition in liquid-phase adsorption/separation. In this review, we give insights into the goal-directed design strategies of organic linkers and metal clusters for the elaboration of task-specific MOFs with targeting effects and high recognition capability, as well as the related progress of MOF-derived membranes. In addition, we put forward the cooperation of different recognition mechanisms to achieve high adsorption/separation performance. At the end, we address the challenges in these fields and propose the prospects and possible evolution for future development. [ABSTRACT FROM AUTHOR]
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- 2019
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11. Engineering electrospun multicomponent polyurethane scaffolding platform comprising grapeseed oil and honey/propolis for bone tissue regeneration.
- Author
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Chao, Cui Yan, Mani, Mohan Prasath, and Jaganathan, Saravana Kumar
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POLYURETHANES , *THERMOGRAVIMETRY , *ATOMIC force microscopy , *ERYTHROCYTES , *MULTIPHASE flow - Abstract
Essential oils play an important role in reducing the pain and inflammation caused by bone fracture.In this study, a scaffold was electrospun based on polyurethane (PU), grape seed oil, honey and propolis for bone tissue-engineering applications. The fiber diameter of the electrospun PU/grape seed oil scaffold and PU/grape seed oil/honey/propolis scaffold were observed to be reduced compared to the pristine PU control. FTIR analysis revealed the existence of grape seed oil, honey and propolis in PU identified by CH band peak shift and also hydrogen bond formation. The contact angle of PU/grape seed oil scaffold was found to increase owing to hydrophobic nature and the contact angle for the PU/grape seed/honey oil/propolis scaffold were decreased because of hydrophilic nature. Further, the prepared PU/grape seed oil and PU/grape seed oil/honey/propolis scaffold showed enhanced thermal stability and reduction in surface roughness than the control as revealed in thermogravimetric analysis (TGA) and atomic force microscopy (AFM) analysis. Further, the developed nanocomposite scaffold displayed delayed blood clotting time than the pristine PU in the activated prothrombin time (APTT) and partial thromboplastin time (PT) assay. The hemolytic assay and cytocompatibility studies revealed that the electrospun PU/grape seed oil and PU/grape seed oil/honey/propolis scaffold possess non-toxic behaviour to red blood cells (RBC) and human fibroblast cells (HDF) cells indicating better blood compatibility and cell viability rates. Hence, the newly developed electrospun nanofibrous composite scaffold with desirable characteristics might be used as an alternative candidate for bone tissue engineering applications. [ABSTRACT FROM AUTHOR]
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- 2018
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12. Physiological analysis of the effect of altitudinal gradients on Leymus secalinus on the Qinghai-Tibetan Plateau.
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Cui, Guowen, Li, Bing, He, Wenhua, Yin, Xiujie, Liu, Shengyong, Lian, Lu, Zhang, Yaling, Liang, Wenxue, and Zhang, Pan
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PEROXIDASE , *SUPEROXIDE dismutase , *MALONDIALDEHYDE , *LIGHT absorption , *REACTIVE oxygen species - Abstract
On the Qinghai-Tibetan Plateau, the high-altitudinal gradients can negatively affect plant distribution and limit plant growth and reproduction. Leymus secalinus (Georgi) Tzvel. is an important forage crop on the Qinghai-Tibetan Plateau and has an excellent ability to fix sand and improve soil. To evaluate the effect of altitude on the physiological characteristics of L. secalinus on the Qinghai-Tibetan Plateau, we measured the lipid peroxidation; chlorophyll a (Chl a), chlorophyll b (Chl b), total carotenoid (Car), soluble protein, proline and soluble sugar contents; and the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in leaves from eight different altitudes in Minhe County and Huangzhong County. The leaves were collected at the initial bloom stage, and the average vertical distance between two adjacent collection sites was approximately 100 meters. The reduction in Chl a and Chl b contents and the increase in Car contents can allow plants to weaken their light absorption and avoid photodamage to the chloroplast. The decrease in malondialdehyde (MDA) content associated with lower lipid peroxidation, and the changes of CAT, SOD and POD activities reflect a higher reactive oxygen species (ROS) scavenging capacity in high-altitude plants. The increase in proline and soluble sugar contents with elevation suggests that proline and soluble sugar may play a key role in the osmotic adjustment of plants in alpine regions. The results suggested that altitudinal gradients negatively affect L. secalinus on the Qinghai-Tibetan Plateau and that the adaptation mechanism and survival strategies of L. secalinus were attributed to the combined effects of multiple protective strategies. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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13. Transcriptome Sequencing and Analysis for Culm Elongation of the World’s Largest Bamboo (Dendrocalamus sinicus).
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Cui, Kai, Wang, Haiying, Liao, Shengxi, Tang, Qi, Li, Li, Cui, Yongzhong, and He, Yuan
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BAMBOO , *FURNITURE , *GENE ontology , *GENES , *PHOTOSYNTHESIS - Abstract
Dendrocalamus sinicus is the world’s largest bamboo species with strong woody culms, and known for its fast-growing culms. As an economic bamboo species, it was popularized for multi-functional applications including furniture, construction, and industrial paper pulp. To comprehensively elucidate the molecular processes involved in its culm elongation, Illumina paired-end sequencing was conducted. About 65.08 million high-quality reads were produced, and assembled into 81,744 unigenes with an average length of 723 bp. A total of 64,338 (79%) unigenes were annotated for their functions, of which, 56,587 were annotated in the NCBI non-redundant protein database and 35,262 were annotated in the Swiss-Prot database. Also, 42,508 and 21,009 annotated unigenes were allocated to gene ontology (GO) categories and clusters of orthologous groups (COG), respectively. By searching against the Kyoto Encyclopedia of Genes and Genomes Pathway database (KEGG), 33,920 unigenes were assigned to 128 KEGG pathways. Meanwhile, 8,553 simple sequence repeats (SSRs) and 81,534 single-nucleotide polymorphism (SNPs) were identified, respectively. Additionally, 388 transcripts encoding lignin biosynthesis were detected, among which, 27 transcripts encoding Shikimate O-hydroxycinnamoyltransferase (HCT) specifically expressed in D. sinicus when compared to other bamboo species and rice. The phylogenetic relationship between D. sinicus and other plants was analyzed, suggesting functional diversity of HCT unigenes in D. sinicus. We conjectured that HCT might lead to the high lignin content and giant culm. Given that the leaves are not yet formed and culm is covered with sheaths during culm elongation, the existence of photosynthesis of bamboo culm is usually neglected. Surprisedly, 109 transcripts encoding photosynthesis were identified, including photosystem I and II, cytochrome b6/f complex, photosynthetic electron transport and F-type ATPase, and 24 transcripts were characterized as antenna proteins that regarded as the main tool for capturing light of plants, implying stem photosynthesis plays a key role during culm elongation due to the unavailability of its leaf. By real-time quantitative PCR, the expression level of 6 unigenes was detected. The results showed the expression level of all genes accorded with the transcriptome data, which confirm the reliability of the transcriptome data. As we know, this is the first study underline the D. sinicus transcriptome, which will deepen the understanding of the molecular mechanisms of culm development. The results may help variety improvement and resource utilization of bamboos. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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14. Inhibition of mitochondrial respiration under hypoxia and increased antioxidant activity after reoxygenation of Tribolium castaneum.
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Wang, Lei, Cui, Sufen, Liu, Zhicheng, Ping, Yong, Qiu, Jiangping, and Geng, Xueqing
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RED flour beetle , *MITOCHONDRIAL physiology , *HYPOXEMIA , *ANTIOXIDANTS , *LACTIC acid - Abstract
Regulating the air in low-oxygen environments protects hermetically stored grains from storage pests damage. However, pests that can tolerate hypoxic stress pose a huge challenge in terms of grain storage. We used various biological approaches to determine the fundamental mechanisms of Tribolium castaneum to cope with hypoxia. Our results indicated that limiting the available oxygen to T. castaneum increased glycolysis and inhibited the Krebs cycle, and that accumulated pyruvic acid was preferentially converted to lactic acid via anaerobic metabolism. Mitochondrial aerobic respiration was markedly suppressed for beetles under hypoxia, which also might have led to mitochondrial autophagy. The enzymatic activity of citrate synthase decreased in insects under hypoxia but recovered within 12 h, which suggested that the beetles recovered from the hypoxia. Moreover, hypoxia-reperfusion resulted in severe oxidative damage to insects, and antioxidant levels increased to defend against the high level of reactive oxygen species. In conclusion, our findings show that mitochondria were the main target in T. castaneum in response to low oxygen. The beetles under hypoxia inhibited mitochondrial respiration and increased antioxidant activity after reoxygenation. Our research advances the field of pest control and makes it possible to develop more efficient strategies for hermetic storage. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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15. A group of Populus trichocarpa DUF231 proteins exhibit differential O-acetyltransferase activities toward xylan.
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Zhong, Ruiqin, Cui, Dongtao, and Ye, Zheng-Hua
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XYLANS , *BLACK cottonwood , *ACETYLTRANSFERASES , *ARABIDOPSIS thaliana , *PLANT biomass , *ACETYLATION - Abstract
Wood represents the most abundant biomass produced by plants and one of its major components is acetyl xylan. Acetylation in xylan can occur at O-2 or O-3 of a xylosyl residue, at both O-2 and O-3 of a xylosyl residue, and at O-3 of a xylosyl residue substituted at O-2 with glucuronic acid. Acetyltransferases responsible for the regiospecific acetylation of xylan in tree species have not yet been characterized. Here we report the biochemical characterization of twelve Populus trichocarpa DUF231-containing proteins, named PtrXOATs, for their roles in the regiospecific acetylation of xylan. The PtrXOAT genes were found to be differentially expressed in Populus organs and among them, PtrXOAT1, PtrXOAT2, PtrXOAT9 and PtrXOAT10 exhibited the highest level of expression in stems undergoing wood formation. Activity assays of recombinant proteins demonstrated that all twelve PtrXOAT proteins were able to transfer acetyl groups from acetyl CoA onto a xylohexaose acceptor with PtrXOAT1, PtrXOAT2, PtrXOAT3, PtrXOAT11 and PtrXOAT12 having the highest activity. Structural analysis of the PtrXOAT-catalyzed reaction products using 1H NMR spectroscopy revealed that PtrXOAT1, PtrXAOT2 and PtrXOAT3 mediated 2-O- and 3-O-monoacetylation and 2,3-di-O-acetylation of xylosyl residues and PtrXOAT11 and PtrXOAT12 only catalyzed 2-O- and 3-O-monoacetylation of xylosyl residues. Of the twelve PtrXOATs, only PtrXOAT9 and PtrXOAT10 were capable of transferring acetyl groups onto the O-3 position of 2-O-glucuronic acid-substituted xylosyl residues. Furthermore, when expressed in the Arabidopsis eskimo1 mutant, PtrXOAT1, PtrXAOT2 and PtrXOAT3 were able to rescue the defects in xylan acetylation. Together, these results demonstrate that the twelve PtrXOATs are acetyltransferases with different roles in xylan acetylation in P. trichocarpa. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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16. Serum metabolome changes in adult patients with severe dengue in the critical and recovery phases of dengue infection.
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Cui, Liang, Pang, Junxiong, Lee, Yie Hou, Ooi, Eng Eong, Ong, Choon Nam, Leo, Yee Sin, and Tannenbaum, Steven R.
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DENGUE , *DENGUE viruses , *METABOLOMICS , *DENGUE hemorrhagic fever , *METABOLITES , *ASPARTATE aminotransferase - Abstract
Dengue virus (DENV) is the most prevalent arbovirus leading to an estimated 100 million symptomatic dengue infections every year. DENV can cause a spectrum of clinical manifestations, ranging from mild dengue fever (DF) to more life threatening forms such as dengue hemorrhagic fever (DHF). The clinical symptoms of DHF become evident typically at the critical phase of infection (5–7 days after onset of fever), yet the mechanisms that trigger transition from DF to DHF are not well understood. We performed a mass spectrometry-based metabolomic profiling of sera from adult DF and DHF patients at the critical and recovery phases of infection. There were 29 differentially expressed metabolites identified between DF and DHF at the critical phase. These include bile acids, purines, acylcarnitines, phospholipids, and amino acids. Bile acids were observed up to 5 fold higher levels among DHF compared to DF patients and were significantly correlated to the higher levels of aspartate transaminase (AST) and alanine transaminase (ALT), suggestive of liver injury among DHF. Uric acid, the most abundant antioxidant in the blood, was observed to be 1.5 fold lower among DHF compared to DF patients. This could result in decreased capacity of endogenous antioxidant defense and elevated oxidative stress among DHF patients. In the recovery phase, the levels of eight metabolites were still significantly higher or lower among DHF patients, including chenodeoxyglycocholic acid, one of the bile acids observed at the critical phase. This indicates potential prolonged adverse impact on the liver due to DENV infection in DHF patients. Our study identified altered metabolic pathways linked to DHF in the critical and recovery phases of dengue infection and provided insights into the different host and DENV interactions between DF and DHF. The results advance our understanding on the mechanisms of DHF pathogenesis, alluding to possible novel therapeutic targets to dengue management. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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17. Deep reconstruction model for dynamic PET images.
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Cui, Jianan, Liu, Xin, Wang, Yile, and Liu, Huafeng
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POSITRON emission tomography , *IMAGE reconstruction , *MAXIMUM likelihood statistics , *PHOTON counting , *DEEP learning - Abstract
Accurate and robust tomographic reconstruction from dynamic positron emission tomography (PET) acquired data is a difficult problem. Conventional methods, such as the maximum likelihood expectation maximization (MLEM) algorithm for reconstructing the activity distribution-based on individual frames, may lead to inaccurate results due to the checkerboard effect and limitation of photon counts. In this paper, we propose a stacked sparse auto-encoder based reconstruction framework for dynamic PET imaging. The dynamic reconstruction problem is formulated in a deep learning representation, where the encoding layers extract the prototype features, such as edges, so that, in the decoding layers, the reconstructed results are obtained through a combination of those features. The qualitative and quantitative results of the procedure, including the data based on a Monte Carlo simulation and real patient data demonstrates the effectiveness of our method. [ABSTRACT FROM AUTHOR]
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- 2017
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18. A novel phloroglucinol and two new phenolic glycosides from Psidium littorale.
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Shu, Ji-Cheng, Cui, Hang-Qing, Huang, Yin-Zheng, Liu, Jian-Qun, and Huang, Hui-Lian
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BIOLOGICAL products , *CHEMISTRY , *GLYCOSIDES , *MEDICINAL plants , *ORGANIC compounds , *PHENOLS , *RESEARCH funding , *SPECTRUM analysis - Abstract
One novel phloroglucinol, psidosone A (1), and two new phenolic glycosides, psidoside A (2), and psidoside B (3), together with nine known phenol compounds (4–12), were isolated from the fruits ofPsidium littoraleRaddi. Their structures were elucidated using data obtained from MS,1H and13C NMR spectra, and correlation experiments (HMQC and HMBC), as well as by comparison with published data. [ABSTRACT FROM AUTHOR]
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- 2017
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19. Proteome dynamics and physiological responses to short-term salt stress in Leymus chinensis leaves.
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Li, Jikai, Cui, Guowen, Hu, Guofu, Wang, Mingjun, Zhang, Pan, Qin, Ligang, Shang, Chen, Zhang, Hailing, Zhu, Xiaocen, and Qu, Mingnan
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- *
PHYSIOLOGICAL effects of salt , *AGRICULTURE , *HYDRAULIC conductivity , *CARBON metabolism , *BIOSYNTHESIS - Abstract
Salt stress is becoming an increasing threat to global agriculture. In this study, physiological and proteomics analysis were performed using a salt-tolerant grass species, Leymus chinensis (L. chinensis). The aim of this study is to understand the potential mechanism of salt tolerance in L. chinensis that used for crop molecular breeding. A series of short-term (<48 h) NaCl treatments (0 ~ 700 mM) were conducted. Physiological data indicated that the root and leaves growth were inhibited, chlorophyll contents decreased, while hydraulic conductivity, proline, sugar and sucrose were accumulated under salt stress. For proteomic analysis, we obtained 274 differentially expressed proteins in response to NaCl treatments. GO analysis revealed that 44 out of 274 proteins are involved in the biosynthesis of amino acids and carbon metabolism. Our findings suggested that L. chinensis copes with salt stress by stimulating the activities of POD, SOD and CAT enzymes, speeding up the reactions of later steps of citrate cycle, and synthesis of proline and sugar. In agreement with our physiological data, proteomic analysis also showed that salt stress depress the expression of photosystem relevant proteins, Calvin cycle, and chloroplast biosynthesis. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
20. Does salt stress affect the interspecific interaction between regionally dominant Suaeda salsa and Scirpus planiculumis?
- Author
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Zhou, Jian, Cui, Lijuan, Pan, Xu, Li, Wei, Zhang, Manyin, and Kang, Xiaoming
- Subjects
- *
ESTUARINE ecology , *PLANT physiology , *ABIOTIC stress , *WETLANDS , *PLANT communities - Abstract
Plant-plant interactions that change along environmental gradients can be affected by different combinations of environmental characteristics, such as the species and planting density ratios. Suaeda salsa and Scirpus planiculumis are regionally dominant species in the Shuangtai estuarine wetland. Compared with non-clonal S. salsa, clonal S. planiculumis has competitive advantages because of its morphological plasticity. However, salt-tolerant S. salsa may grow faster than S. planiculumis in saline-alkali estuary soil. Whether the interactions between these two species along salinity gradients are affected by the level of salt stress and mixed planting density ratio remains unclear. Thus, to test the effects of salt stress and planting density ratios on the interactions between S. planiculumis and S. salsa in the late growing season, we conducted a greenhouse experiment consisting of 3 salinity levels (0, 8 and 15ppt) and 5 planting density ratios. Our results showed that the promotion of S. salsa growth and inhibition of S. planiculumis growth at low salinity levels (8 ppt) did not alter the interactions between the two species. Facilitation of S. salsa occurred at high salinity levels, and the magnitude of this net outcome decreased with increases in the proportion of S. salsa. These results suggest that competition and facilitation processes not only depend on the combinations of different life-history characteristics of species but also on the planting density ratio. These findings may contribute to the understanding of the responses of estuarine wetland plant-plant interactions to human modifications of estuarine salinity. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
21. Comparative analysis of the expression level of recombinant ginsenoside-transforming β-glucosidase in GRAS hosts and mass production of the ginsenoside Rh2-Mix.
- Author
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Siddiqi, Muhammad Zubair, Cui, Chang-Hao, Park, Seul-Ki, Han, Nam Soo, Kim, Sun-Chang, and Im, Wan-Taek
- Subjects
- *
GINSENOSIDES , *GLUCOSIDASES , *ANTINEOPLASTIC agents , *ESCHERICHIA coli , *SACCHAROMYCES cerevisiae - Abstract
The ginsenoside Rh2, a pharmaceutically active component of ginseng, is known to have anticancer and antitumor effects. However, white ginseng and red ginseng have extremely low concentrations of Rh2 or Rh2-Mix [20(S)-Rh2, 20(R)-Rh2, Rk2, and Rh3]. To enhance the production of food-grade ginsenoside Rh2, an edible enzymatic bioconversion technique was developed adopting GRAS host strains. A β-glucosidase (BglPm), which has ginsenoside conversion ability, was expressed in three GRAS host strains (Corynebacterium glutamicum, Saccharomyces cerevisiae and Lactococus lactis) by using a different vector system. Enzyme activity in these three GRAS hosts were 75.4%, 11.5%, and 9.3%, respectively, compared to that in the E. coli pGEX 4T-1 expression system. The highly expressed BglPm_C in C. glutamicum can effectively transform the ginsenoside Rg3-Mix [20(S)-Rg3, 20(R)-Rg3, Rk1, Rg5] to Rh2-Mix [20(S)-Rh2, 20(R)-Rh2, Rk2, Rh3] using a scaled-up biotransformation reaction, which was performed in a 10-L jar fermenter at pH 6.5/7.0 and 37°C for 24 h. To our knowledge, this is the first report in which 50 g of PPD-Mix (Rb1, Rb2, Rb3, Rc, and Rd) as a starting substrate was converted to ginsenoside Rg3-Mix by acid heat treatment and then 24.5-g Rh2-Mix was obtained by enzymatic transformation of Rg3-Mix through by BglPm_C. Utilization of this enzymatic method adopting a GRAS host could be usefully exploited in the preparation of ginsenoside Rh2-Mix in cosmetics, functional food, and pharmaceutical industries, thereby replacing the E. coli expression system. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
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22. SENP7 Potentiates cGAS Activation by Relieving SUMO-Mediated Inhibition of Cytosolic DNA Sensing.
- Author
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Cui, Ye, Yu, Huansha, Zheng, Xin, Peng, Rui, Wang, Qiang, Zhou, Yi, Wang, Rui, Wang, Jiehua, Qu, Bo, Shen, Nan, Guo, Qiang, Liu, Xing, and Wang, Chen
- Subjects
- *
SYNTHASES , *PROTEOLYTIC enzymes , *OLIGOMERIZATION , *NUCLEOTIDYLTRANSFERASES , *NATURAL immunity - Abstract
Cyclic GMP-AMP (cGAMP) synthase (cGAS, a.k.a. MB21D1), a cytosolic DNA sensor, catalyzes formation of the second messenger 2’3’-cGAMP that activates the stimulator of interferon genes (STING) signaling. How the cGAS activity is modulated remains largely unknown. Here, we demonstrate that sentrin/SUMO-specific protease 7 (SENP7) interacted with and potentiated cGAS activation. The small ubiquitin-like modifier (SUMO) was conjugated onto the lysine residues 335, 372 and 382 of cGAS, which suppressed its DNA-binding, oligomerization and nucleotidyl-transferase activities. SENP7 reversed this inhibition via catalyzing the cGAS de-SUMOylation. Consistently, silencing of SENP7 markedly impaired the IRF3-responsive gene expression induced by cGAS-STING axis. SENP7-knockdown mice were more susceptible to herpes simplex virus 1 (HSV-1) infection. SENP7 was significantly up-regulated in patients with SLE. Our study highlights the temporal modulation of the cGAS activity via dynamic SUMOylation, uncovering a novel mechanism for fine-tuning the STING signaling in innate immunity. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
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23. Effect of Straw Amendment on Soil Zn Availability and Ageing of Exogenous Water-Soluble Zn Applied to Calcareous Soil.
- Author
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Chen, Yanlong, Cui, Juan, Tian, Xiaohong, Zhao, Aiqing, Li, Meng, Wang, Shaoxia, Li, Xiushaung, Jia, Zhou, and Liu, Ke
- Subjects
- *
ZINC , *SOIL composition , *SOIL amendments , *CALCAREOUS soils , *HUMUS , *WHEAT straw - Abstract
Organic matter plays a key role in availability and transformation of soil Zn (zinc), which greatly controls Zn concentrations in cereal grains and human Zn nutrition level. Accordingly, soils homogenized with the wheat straw (0, 12 g straw kg-1) and Zn fertilizer (0, 7 mg Zn kg-1) were buried and incubated in the field over 210 days to explore the response of soil Zn availability and the ageing of exogenous Zn to straw addition. Results indicated that adding straw alone scarcely affected soil DTPA-Zn concentration and Zn fractions because of the low Zn concentration of wheat straw and the high soil pH, and large clay and calcium carbonate contents. However, adding exogenous Zn plus straw increased the DTPA-Zn abundance by about 5-fold and had the similar results to adding exogenous Zn alone, corresponding to the increased Zn fraction loosely bounded to organic matter, which had a more dominant presence in Zn reaction than soil other constituents such as carbonate and minerals in calcareous soil. The higher relative amount of ineffective Zn (~50%) after water soluble Zn addition also occurred, and at the days of 120–165 and 180–210when the natural temperature and rainfall changed mildly, the ageing process of exogenous Zn over time was well evaluated by the diffusion equation, respectively. Consequently, combining crop residues with exogenous water soluble Zn application is promising strategy to maximize the availability of Zn in calcareous soil, but the higher ageing rate of Zn caused by the higher Zn mobility should be considered. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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24. Fabrication of (Co,Mn)3O4/rGO Composite for Lithium Ion Battery Anode by a One-Step Hydrothermal Process with H2O2 as Additive.
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Li, Zuohua, Cui, Yanhui, Chen, Jun, Deng, Lianlin, and Wu, Junwei
- Subjects
- *
LITHIUM-ion batteries , *TRANSITION metal oxides , *FABRICATION (Manufacturing) , *ADDITIVES , *ENERGY storage , *ELECTRODES - Abstract
Binary transition metal oxides have been regarded as one of the most promising candidates for high-performance electrodes in energy storage devices, since they can offer high electrochemical activity and high capacity. Rational designing nanosized metal oxide/carbon composite architectures has been proven to be an effective way to improve the electrochemical performance. In this work, the (Co,Mn)3O4 spinel was synthesized and anchored on reduced graphene oxide (rGO) nanosheets using a facile and single hydrothermal step with H2O2 as additive, no further additional calcination required. Analysis showed that this method gives a mixed spinel, i.e. (Co,Mn)3O4, having 2+ and 3+ Co and Mn ions in both the octahedral and tetrahedral sites of the spinel structure, with a nanocubic morphology roughly 20 nm in size. The nanocubes are bound onto the rGO nanosheet uniformly in a single hydrothermal process, then the as-prepared (Co,Mn)3O4/rGO composite was characterized as the anode materials for Li-ion battery (LIB). It can deliver 1130.6 mAh g-1 at current density of 100 mA g-1 with 98% of coulombic efficiency after 140 cycles. At 1000 mA g-1, the capacity can still maintain 750 mAh g-1, demonstrating excellent rate capabilities. Therefore, the one-step process is a facile and promising method to fabricate metal oxide/rGO composite materials for energy storage applications. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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25. Cr(VI) Adsorption on Red Mud Modified by Lanthanum: Performance, Kinetics and Mechanisms.
- Author
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Cui, You-Wei, Li, Jie, Du, Zhao-Fu, and Peng, Yong-Zhen
- Subjects
- *
CHROMIUM in water , *METAL absorption & adsorption , *LANTHANUM , *WATER pollution , *ECOLOGICAL risk assessment , *HEALTH risk assessment - Abstract
Water pollution caused by the highly toxic metal hexavalent chromium (Cr(VI)) creates significant human health and ecological risks. In this study, a novel adsorbent was used to treat Cr(VI)-containing wastewater; the adsorbent was prepared using red mud (RM) generated from the alumina production industry and the rare earth element lanthanum. This study explored adsorption performance, kinetics, and mechanisms. Results showed that the adsorption kinetics of the RM modified by lanthanum (La-RM), followed the pseudo-second-order model, with a rapid adsorption rate. Cr(VI) adsorption was positively associated with the absorbent dose, pH, temperature, and initial Cr(VI) concentration; coexisting anions had little impact. The maximum Cr(VI) adsorption capacity was 17.35 mg/g. Cr(VI) adsorption on La-RM was a mono-layer adsorption pattern, following the Langmuir isotherm model. Thermodynamic parameters showed the adsorption was spontaneous and endothermic. The adsorption of Cr(VI) on La-RM occurred as a result of LaOCl formation on the RM surface, which in turn further reacted with Cr(VI) in the wastewater. This study highlighted a method for converting industrial waste into a valuable material for wastewater treatment. The novel absorbent could be used as a potential adsorbent for treating Cr(VI)-contaminating wastewater, due to its cost-effectiveness and high adsorption capability. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
26. Glucose Sensor MdHXK1 Phosphorylates and Stabilizes MdbHLH3 to Promote Anthocyanin Biosynthesis in Apple.
- Author
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Hu, Da-Gang, Sun, Cui-Hui, Zhang, Quan-Yan, An, Jian-Ping, You, Chun-Xiang, and Hao, Yu-Jin
- Subjects
- *
APPLES , *BIOSYNTHESIS , *ANTHOCYANINS , *GLUCOSE , *TRANSCRIPTION factors - Abstract
Glucose induces anthocyanin accumulation in many plant species; however, the molecular mechanism involved in this process remains largely unknown. Here, we found that apple hexokinase MdHXK1, a glucose sensor, was involved in sensing exogenous glucose and regulating anthocyanin biosynthesis. In vitro and in vivo assays suggested that MdHXK1 interacted directly with and phosphorylated an anthocyanin-associated bHLH transcription factor (TF) MdbHLH3 at its Ser361 site in response to glucose. Furthermore, both the hexokinase_2 domain and signal peptide are crucial for the MdHXK1-mediated phosphorylation of MdbHLH3. Moreover, phosphorylation modification stabilized MdbHLH3 protein and enhanced its transcription of the anthocyanin biosynthesis genes, thereby increasing anthocyanin biosynthesis. Finally, a series of transgenic analyses in apple calli and fruits demonstrated that MdHXK1 controlled glucose-induced anthocyanin accumulation at least partially, if not completely, via regulating MdbHLH3. Overall, our findings provide new insights into the mechanism of the glucose sensor HXK1 modulation of anthocyanin accumulation, which occur by directly regulating the anthocyanin-related bHLH TFs in response to a glucose signal in plants. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
27. Sterigmatocystin-Induced DNA Damage Triggers G2 Arrest via an ATM/p53-Related Pathway in Human Gastric Epithelium GES-1 Cells In Vitro
- Author
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Zhang, Donghui, Cui, Yu, Shen, Haitao, Xing, Lingxiao, Cui, Jinfeng, Wang, Juan, and Zhang, Xianghong
- Subjects
- *
STERIGMATOCYSTIN , *DNA damage , *FOOD toxicology , *EPITHELIUM , *IN vitro studies , *TOXIGENIC fungi , *HUMAN carcinogenesis , *CELLULAR signal transduction , *DISEASES - Abstract
Sterigmatocystin (ST), which is commonly detected in food and feed commodities, is a mutagenic and carcinogenic mycotoxin that has been recognized as a possible human carcinogen. Our previous study showed that ST can induce G2 phase arrest in GES-1 cells in vitro and that the MAPK and PI3K signaling pathways are involved in the ST-induced G2 arrest. It is now widely accepted that DNA damage plays a critical role in the regulation of cell cycle arrest and apoptosis. In response to DNA damage, a complex signaling network is activated in eukaryotic cells to trigger cell cycle arrest and facilitate DNA repair. To further explore the molecular mechanism through which ST induces G2 arrest, the current study was designed to precisely dissect the role of DNA damage and the DNA damage sensor ataxia telangiectasia-mutated (ATM)/p53-dependent pathway in the ST-induced G2 arrest in GES-1 cells. Using the comet assay, we determined that ST induces DNA damage, as evidenced by the formation of DNA comet tails, in GES-1 cells. We also found that ST induces the activation of ATM and its downstream molecules, Chk2 and p53, in GES-1 cells. The ATM pharmacological inhibitor caffeine was found to effectively inhibit the activation of the ATM-dependent pathways and to rescue the ST-induced G2 arrest in GES-1 cells, which indicating its ATM-dependent characteristic. Moreover, the silencing of the p53 expression with siRNA effectively attenuated the ST-induced G2 arrest in GES-1 cells. We also found that ST induces apoptosis in GES-1 cells. Thus, our results show that the ST-induced DNA damage activates the ATM/53-dependent signaling pathway, which contributes to the induction of G2 arrest in GES-1 cells. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
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28. Hydrothermal syntheses and characterizations of two novel heteropolytungstate supported coordination compounds
- Author
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Cui, Ji-Wen, Cui, Xiao-Bing, Yu, Hai-Hui, Xu, Ji-Qing, Yi, Zhi-Hui, and Duan, Wei-Jie
- Subjects
- *
ORGANIC compounds , *SPECTRUM analysis , *PHYSICAL & theoretical chemistry , *CHEMISTRY - Abstract
Abstract: Two novel heteropolytungstates, [Ni(2,2′-bpy)3]1.5{PW10.79V1.21O40(VO)0.5[Ni(2,2′-bpy)2L]}·0.5H2O (1) (2,2′-bpy=2,2′-bipyridine, L=0.5H2O+0.5OH−) and K[PW12O40[Ni(1,10-phen)2(OH)]2]·2H2O (2) (1,10-phen=1,10-phenanthroline), have been synthesized under hydrothermal conditions and characterized by IR, XPS, and X-ray diffraction analyses. The polyoxoanion of 1 is a mono-capped α-Keggin cluster which supports a metal coordination fragment [Ni(2,2′-bpy)2L]1.5+, while the structure of 2 exhibits a 1D chain constructed from pseudo-Keggin cluster bi-supported transition metal complexes linked by K+ ions. Studies of magnetic properties indicated the presence of paramagnetic behaviours for compounds 1 and 2. [Copyright &y& Elsevier]
- Published
- 2008
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29. Benzimidazobenzothiazole-Based Bipolar Hosts to Harvest Nearly All of the Excitons from Blue Delayed Fluorescence and Phosphorescent Organic Light-Emitting Diodes.
- Author
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Cui, Lin-Song, Kim, Jong Uk, Nomura, Hiroko, Nakanotani, Hajime, and Adachi, Chihaya
- Subjects
- *
ORGANIC light emitting diodes , *PHOSPHORESCENCE , *DELAYED fluorescence , *QUANTUM efficiency , *QUANTUM chemistry , *CHEMISTRY - Abstract
Much effort has been devoted to developing highly efficient organic light-emitting diodes (OLEDs) that function through phosphorescence or thermally activated delayed fluorescence (TADF). However, efficient host materials for blue TADF and phosphorescent guest emitters are limited because of their requirement of high triplet energy levels. Herein, we report the rigid acceptor unit benzimidazobenzothiazole (BID-BT), which is suitable for use in bipolar hosts in blue OLEDs. The designed host materials, based on BID-BT, possess high triplet energy and bipolar carrier transport ability. Both blue TADF and phosphorescent OLEDs containing BID-BT-based derivatives exhibit external quantum efficiencies as high as 20 %, indicating that these hosts allow efficient triplet exciton confinement appropriate for blue TADF and phosphorescent guest emitters. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
30. Changes in Biomass and Quality of Alpine Steppe in Response to N & P Fertilization in the Tibetan Plateau.
- Author
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Dong, Junfu, Cui, Xiaoyong, Wang, Shuping, Wang, Fang, Pang, Zhe, Xu, Ning, Zhao, Guoqiang, and Wang, Shiping
- Subjects
- *
MOUNTAIN plants , *BIOMASS , *FERTILIZATION (Biology) , *NITROGEN & the environment , *PHOSPHORUS & the environment - Abstract
In the alpine steppe zone on the Central Tibetan Plateau, a large amount of area has been degraded due to natural and artificial factors. N & P fertilization is widely accepted to recover degraded pastures in other regions all over the world. However, it is not clear how alpine steppe communities respond to N & P fertilization, and what is the optimal application rate, in the perspective of forage production. To attempt to explore these questions, in July 2013, two fencing sites were designed in Baingoin County with 12 treatments of different levels of nitrogen (N0: 0; N1: 7.5 g m-2 yr-1; N2: 15 g m-2 yr-1) & phosphate (P0: 0; P1: 7.5 gP2O5 m-2 yr-1; P2: 15 gP2O5 m-2 yr-1; P3: 30 gP2O5 m-2 yr-1). The results indicated N&P addition was capable to ameliorate the quality of the two sites in the Tibetan Plateau steppe. Increasing N application level resulted in significant increment in Gramineae and total biomass in the two sites. P addition significantly improved the quantity of Compositae, total biomass and the biomasss of other species in site II, while it only significantly improved the total biomass in site I. Gramineae was much more sensitive to N-induced changes than P-induced changes, and this indicated N addition was better to ameliorate the quality of plateau steppe than P-induced changes. No strong evidence was found for critical threshold within 15 g N m-2 yr-1, and there was decreasing tendency when P addition rate was above 15 g m-2 yr-1. N&P has the potential to accelerate soil acidification, which improved the content of available K, likely as a result of nonsignificant correlation between biomass and soil moisture. This work highlights the the tradeoffs that exist in N and P addition in recovering degraded steppe. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
31. Plant Density Effect on Grain Number and Weight of Two Winter Wheat Cultivars at Different Spikelet and Grain Positions.
- Author
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Li, Yong, Cui, Zhengyong, Ni, Yingli, Zheng, Mengjing, Yang, Dongqing, Jin, Min, Chen, Jin, Wang, Zhenlin, and Yin, Yanping
- Subjects
- *
PLANT spacing , *WHEAT varieties , *CULTIVATORS , *WHEAT yields , *PLANT growth - Abstract
In winter wheat, grain development is asynchronous. The grain number and grain weight vary significantly at different spikelet and grain positions among wheat cultivars grown at different plant densities. In this study, two winter wheat (Triticum aestivum L.) cultivars, ‘Wennong6’ and ‘Jimai20’, were grown under four different plant densities for two seasons, in order to study the effect of plant density on the grain number and grain weight at different spikelet and grain positions. The results showed that the effects of spikelet and grain positions on grain weight varied with the grain number of spikelets. In both cultivars, the single-grain weight of the basal and middle two-grain spikelets was higher at the 2nd grain position than that at the 1st grain position, while the opposite occurred in the top two-grain spikelets. In the three-grain spikelets, the distribution of the single-grain weight was different between cultivars. In the four-grain spikelets of Wennong6, the single-grain weight was the highest at the 2nd grain position, followed by the 1st, 3rd, and 4th grain positions. Regardless of the spikelet and grain positions, the single-grain weight was the highest at the 1st and 2nd grain positions and the lowest at the 3rd and 4th grain positions. Overall, plant density affected the yield by controlling the seed-setting characteristics of the tiller spike. Therefore, wheat yield can be increased by decreasing the sterile basal and top spikelets and enhancing the grain weight at the 3rd and 4th grain positions, while maintaining it at the 1st and 2nd grain positions on the spikelet. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
32. Exploration of Stereoselectivity in Embryo-Larvae (Danio rerio) Induced by Chiral PCB149 at the Bioconcentration and Gene Expression Levels.
- Author
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Chai, Tingting, Cui, Feng, Mu, Xiyan, Yang, Yang, Wang, Chengju, and Qiu, Jing
- Subjects
- *
ZEBRA danio , *GENE expression , *BIOCONCENTRATION , *ISOMERS , *ISOMERIZATION - Abstract
This paper was designed to study stereoselective enrichment and changes in gene expression when zebrafish (Danio rerio) embryo-larvae were exposed to racemic, (-)- or (+)- PCB149 (2,2’,3,4’,5’,6- hexachlorobiphenyl). Based on bioconcentration analysis, non-racemic enrichment was significantly observed after racemic exposure. No isomerization between the two isomers was found after (-)/(+)-PCB149 exposure. Furthermore, based on gene expression-data mining, CYPs genes (cyp2k6, cyp19a1b, and cyp2aa4) were differential genes after (+)-PCB149 exposure. No obvious differences of dysregulation of gene expression caused by racemic and (-)-PCB149, were observed in embryo-larvae. The above results suggested that (-)-PCB149 could be considered as the main factor causing the dysregulation of gene expression in embryo-larvae after racemic exposure; and (+)-PCB149 should be pursued apart from the racemate, when considering the toxicity of chiral PCB149. Thus, the information in our study could provide new insights to assess the environmental risk of chiral PCBs in aquatic systems. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
33. Modeling of Mechanical Stress Exerted by Cholesterol Crystallization on Atherosclerotic Plaques.
- Author
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Luo, Yuemei, Cui, Dongyao, Yu, Xiaojun, Chen, Si, Liu, Xinyu, Tang, Hongying, Wang, Xianghong, and Liu, Linbo
- Subjects
- *
ATHEROSCLEROTIC plaque , *CHOLESTEROL , *STRAINS & stresses (Mechanics) , *CRYSTALLIZATION , *THROMBOSIS , *FINITE element method - Abstract
Plaque rupture is the critical cause of cardiovascular thrombosis, but the detailed mechanisms are not fully understood. Recent studies have found abundant cholesterol crystals in ruptured plaques, and it has been proposed that the rapid expansion of cholesterol crystals in a limited space during crystallization may contribute to plaque rupture. To evaluate the effect of cholesterol crystal growth on atherosclerotic plaques, we modeled the expansion of cholesterol crystals during the crystallization process in the necrotic core and estimated the stress on the thin cap with different arrangements of cholesterol crystals. We developed a two-dimensional finite element method model of atherosclerotic plaques containing expanding cholesterol crystals and investigated the effect of the magnitude and distribution of crystallization on the peak circumferential stress born by the cap. Using micro-optical coherence tomography (μOCT), we extracted the cross-sectional geometric information of cholesterol crystals in human atherosclerotic aorta tissue ex vivo and applied the information to the model. The results demonstrate that (1) the peak circumference stress is proportionally dependent on the cholesterol crystal growth; (2) cholesterol crystals at the cap shoulder impose the highest peak circumference stress; and (3) spatial distributions of cholesterol crystals have a significant impact on the peak circumference stress: evenly distributed cholesterol crystals exert less peak circumferential stress on the cap than concentrated crystals. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
34. Metabolomics Revealed an Association of Metabolite Changes and Defective Growth in Methylobacterium extorquens AM1 Overexpressing ecm during Growth on Methanol.
- Author
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Cui, Jinyu, Good, Nathan M., Hu, Bo, Yang, Jing, Wang, Qianwen, Sadilek, Martin, and Yang, Song
- Subjects
- *
METABOLOMICS , *METABOLITES , *METHYLOBACTERIUM extorquens , *BACTERIAL growth , *METHANOL - Abstract
Methylobacterium extorquens AM1 is a facultative methylotroph capable of growth on both single-carbon and multi-carbon compounds. The ethylmalonyl-CoA (EMC) pathway is one of the central assimilatory pathways in M. extorquens during growth on C1 and C2 substrates. Previous studies had shown that ethylmalonyl-CoA mutase functioned as a control point during the transition from growth on succinate to growth on ethylamine. In this study we overexpressed ecm, phaA, mcmAB and found that upregulating ecm by expressing it from the strong constitutive mxaF promoter caused a 27% decrease in growth rate on methanol compared to the strain with an empty vector. Targeted metabolomics demonstrated that most of the central intermediates in the ecm over-expressing strain did not change significantly compared to the control strain; However, poly-β-hydroxybutyrate (PHB) was 4.5-fold lower and 3-hydroxybutyryl-CoA was 1.6-fold higher. Moreover, glyoxylate, a toxic and highly regulated essential intermediate, was determined to be 2.6-fold higher when ecm was overexpressed. These results demonstrated that overexpressing ecm can manipulate carbon flux through the EMC pathway and divert it from the carbon and energy storage product PHB, leading to an accumulation of glyoxylate. Furthermore, untargeted metabolomics discovered two unusual metabolites, alanine (Ala)– (mDAP) and Ala–mDAP–Ala, each over 45-fold higher in the ecm over-expressing strain. These two peptides were also found to be highly produced in a dose-dependent manner when glyoxylate was added to the control strain. Overall, this work has explained a direct association of ecm overexpression with glyoxylate accumulation up to a toxic level, which inhibits cell growth on methanol. This research provides useful insight for manipulating the EMC pathway for efficiently producing high-value chemicals in M. extorquens. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
35. PSIONplus: Accurate Sequence-Based Predictor of Ion Channels and Their Types.
- Author
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Gao, Jianzhao, Cui, Wei, Sheng, Yajun, Ruan, Jishou, and Kurgan, Lukasz
- Subjects
- *
AMINO acid sequence , *ION channels , *MOLECULAR structure , *MEMBRANE proteins , *DRUG synergism , *TARGETED drug delivery - Abstract
Ion channels are a class of membrane proteins that attracts a significant amount of basic research, also being potential drug targets. High-throughput identification of these channels is hampered by the low levels of availability of their structures and an observation that use of sequence similarity offers limited predictive quality. Consequently, several machine learning predictors of ion channels from protein sequences that do not rely on high sequence similarity were developed. However, only one of these methods offers a wide scope by predicting ion channels, their types and four major subtypes of the voltage-gated channels. Moreover, this and other existing predictors utilize relatively simple predictive models that limit their accuracy. We propose a novel and accurate predictor of ion channels, their types and the four subtypes of the voltage-gated channels called PSIONplus. Our method combines a support vector machine model and a sequence similarity search with BLAST. The originality of PSIONplus stems from the use of a more sophisticated machine learning model that for the first time in this area utilizes evolutionary profiles and predicted secondary structure, solvent accessibility and intrinsic disorder. We empirically demonstrate that the evolutionary profiles provide the strongest predictive input among new and previously used input types. We also show that all new types of inputs contribute to the prediction. Results on an independent test dataset reveal that PSIONplus obtains relatively good predictive performance and outperforms existing methods. It secures accuracies of 85.4% and 68.3% for the prediction of ion channels and their types, respectively, and the average accuracy of 96.4% for the discrimination of the four ion channel subtypes. Standalone version of PSIONplus is freely available from [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
36. Steroidal Saponins from the Rhizomes of Aspidistra typica.
- Author
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Cui, Jiang-Ming, Kang, Li-Ping, Zhao, Yang, Zhao, Jian-Yuan, Zhang, Jie, Pang, Xu, Yu, He-Shui, Jia, De-Xian, Liu, Chao, Yu, Li-Yan, and Ma, Bai-Ping
- Subjects
- *
ASPARAGACEAE , *SAPONINS , *REPRODUCTIVE isolation in plants , *MASS spectrometry , *CHEMICAL structure , *GLYCOSIDES - Abstract
Eleven new furostanol saponins, typaspidosides B-L (1–11), one new spirostanol saponin, typaspidoside M (12), and five known spirostanol saponins, 25S-atropuroside (13), neoaspidistrin (14), (25S)-pratioside D1 (15), 25S-aspidistrin (16) and 25S-neosibiricoside (17) were isolated from the rhizomes of Aspidistra typica Baill. The structures of the new compounds were established using 1D and 2D NMR (1H-1H COSY, HMQC, HMBC and ROESY) spectroscopy, high resolution mass spectrometry, and chemical methods. The aglycones of 1–3 (unusual furostanol saponins with opened E ring type), 9 and 10 (the methoxyl substituent at C-23 position) were found, identified from natural products for the first time. Moreover, the anti-HIV activities of the isolated steroidal glycosides were assessed, and compounds 13, 14, 16 and 17 exhibited high active against HIV-1. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
37. Novel Pectate Lyase Genes of Heterodera glycines Play Key Roles in the Early Stage of Parasitism.
- Author
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Peng, Huan, Cui, Jiangkuan, Long, Haibo, Huang, Wenkun, Kong, Lingan, Liu, Shiming, He, Wenting, Hu, Xianqi, and Peng, Deliang
- Subjects
- *
PECTATE lyase , *SOYBEAN cyst nematode , *PARASITISM , *ANTISENSE DNA , *AMINO acid sequence , *GENE expression , *SOUTHERN blot - Abstract
Pectate lyases are known to play a key role in pectin degradation by catalyzing the random cleavage of internal polymer linkages (endo-pectinases). In this paper, four novel cDNAs, designated Hg-pel-3, Hg-pel-4, Hg-pel-6 and Hg-pel-7, that encode pectate lyases were cloned and characterized from the soybean cyst nematode, Heterodera glycines. The predicted protein sequences of HG-PEL-3, HG-PEL-4 and HG-PEL-6 differed significantly in both their amino acid sequences and their genomic structures from other pectate lyases of H. glycines (HG-PEL-1, HG-PEL-2 and HG-PEL-7). A phylogenetic study revealed that the pectate lyase proteins of H. glycines are clustered into distinct clades and have distinct numbers and positioning of introns, which suggests that the pectate lyase genes of H. glycines may have evolved from at least two ancestral genes. A Southern blot analysis revealed that multiple Hg-pel-6-like genes were present in the H. glycines genome. In situ hybridization showed that four novel pectate lyases (Hg-pel-3, Hg-pel-4, Hg-pel-6 and Hg-pel-7) were actively transcribed in the subventral esophageal gland cells. A semi-quantitative RT-PCR assay supported the finding that the expression of these genes was strong in the egg, pre-parasitic second-stage juvenile (J2) and early parasitic J2 stages and that it declined in further developmental stages of the nematode. This expression pattern suggests that these proteins play a role in the migratory phase of the nematode life cycle. Knocking down Hg-pel-6 using in vitro RNA interference resulted in a 46.9% reduction of the number of nematodes that invaded the plants and a 61.5% suppression of the development of H. glycines females within roots compared to the GFP-dsRNA control. Plant host-derived RNAi induced the silencing of the Hg-pel-6gene, which significantly reduced the nematode infection levels at 7 Days post inoculation (dpi). Similarly, this procedure reduced the number of female adults at 40 dpi, which suggests the important roles of this gene in the early stages of parasitism. Our combined data suggest that two types of pectate lyases are present in the H. glycines genome and may have different roles during infection. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
38. Evaluating the Applicability of Phi Coefficient in Indicating Habitat Preferences of Forest Soil Fauna Based on a Single Field Study in Subtropical China.
- Author
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Cui, Yang, Wang, Silong, and Yan, Shaokui
- Subjects
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SOIL ecology , *FOREST animals , *SOIL animals , *HABITATS , *COEFFICIENTS (Statistics) , *EXPERIMENTAL agriculture - Abstract
Phi coefficient directly depends on the frequencies of occurrence of organisms and has been widely used in vegetation ecology to analyse the associations of organisms with site groups, providing a characterization of ecological preference, but its application in soil ecology remains rare. Based on a single field experiment, this study assessed the applicability of phi coefficient in indicating the habitat preferences of soil fauna, through comparing phi coefficient-induced results with those of ordination methods in charactering soil fauna-habitat(factors) relationships. Eight different habitats of soil fauna were implemented by reciprocal transfer of defaunated soil cores between two types of subtropical forests. Canonical correlation analysis (CCorA) showed that ecological patterns of fauna-habitat relationships and inter-fauna taxa relationships expressed, respectively, by phi coefficients and predicted abundances calculated from partial redundancy analysis (RDA), were extremely similar, and a highly significant relationship between the two datasets was observed (Pillai's trace statistic = 1.998, P = 0.007). In addition, highly positive correlations between phi coefficients and predicted abundances for Acari, Collembola, Nematode and Hemiptera were observed using linear regression analysis. Quantitative relationships between habitat preferences and soil chemical variables were also obtained by linear regression, which were analogous to the results displayed in a partial RDA biplot. Our results suggest that phi coefficient could be applicable on a local scale in evaluating habitat preferences of soil fauna at coarse taxonomic levels, and that the phi coefficient-induced information, such as ecological preferences and the associated quantitative relationships with habitat factors, will be largely complementary to the results of ordination methods. The application of phi coefficient in soil ecology may extend our knowledge about habitat preferences and distribution-abundance relationships, which will benefit the understanding of biodistributions and variations in community compositions in the soil. Similar studies in other places and scales apart from our local site will be need for further evaluation of phi coefficient. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
39. Syntheses of Chiral Ferrocenophanes and Their Application to Asymmetric Catalysis.
- Author
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Zhang, Qiying, Cui, Xiuling, Chen, Lianmei, Liu, Haitao, and Wu, Yangjie
- Subjects
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CHEMICAL synthesis , *CATALYSIS research , *CHEMISTRY , *ORGANOCATALYSIS , *ALDEHYDES - Abstract
N-Substituted 2-aza-[3]-ferrocenophanes were easily synthesized from 1,1′-ferrocenedicarbaldehyde and aliphatic amines in high yields. One of the ferrocenophanes served as a ligand for the copper-catalyzed oxidative coupling of 2-naphthol derivatives to give the products in good yields with up to 92 % ee, and it also efficiently catalyzed the asymmetric Michael addition reaction as an organocatalyst. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
40. Heavy Metal Accumulation by Periphyton Is Related to Eutrophication in the Hai River Basin, Northern China.
- Author
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Tang, Wenzhong, Cui, Jingguo, Shan, Baoqing, Wang, Chao, and Zhang, Wenqiang
- Subjects
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HEAVY metals , *FOOD chains , *FILAMENTOUS bacteria , *POPULATION density , *DIATOMS , *EUTROPHICATION - Abstract
The Hai River Basin (HRB) is one of the most polluted river basins in China. The basin suffers from various types of pollutants including heavy metals and nutrients due to a high population density and rapid economic development in this area. We assessed the relationship between heavy metal accumulation by periphyton playing an important role in fluvial food webs and eutrophication in the HRB. The concentrations of the unicellular diatoms (type A), filamentous algae with diatoms (type B), and filamentous algae (type C) varied along the river, with type A dominating upstream, and types B then C increasing in concentration further downstream, and this was consistent with changes in the trophic status of the river. The mean heavy metal concentrations in the type A, B and C organisms were Cr: 18, 18 and 24 mg/kg, respectively, Ni: 9.2, 10 and 12 mg/kg, respectively, Cu: 8.4, 19 and 29 mg/kg, respectively, and Pb: 11, 9.8 and 7.1 mg/kg respectively. The bioconcentration factors showed that the abilities of the organisms to accumulate Cr, Ni and Pb decreased in the order type A, type B, then type C, but their abilities to accumulate Cu increased in that order. The Ni concentration was a good predictor of Cr, Cu and Pb accumulation by all three periphyton types. Our study shows that heavy metal accumulation by periphyton is associated with eutrophication in the rivers in the HRB. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
41. Serum Metabolome and Lipidome Changes in Adult Patients with Primary Dengue Infection.
- Author
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Cui, Liang, Lee, Yie Hou, Kumar, Yadunanda, Xu, Fengguo, Lu, Kun, Ooi, Eng Eong, Tannenbaum, Steven R., and Ong, Choon Nam
- Subjects
- *
METABOLOMICS , *DENGUE , *LIQUID chromatography , *OXIDATION , *PHOSPHOLIPIDS , *METABOLISM , *DISEASE risk factors - Abstract
Background: Dengue virus (DENV) is the most widespread arbovirus with an estimated 100 million infections occurring every year. Endemic in the tropical and subtropical areas of the world, dengue fever/dengue hemorrhagic fever (DF/DHF) is emerging as a major public health concern. The complex array of concurrent host physiologic changes has hampered a complete understanding of underlying molecular mechanisms of dengue pathogenesis. Methodology/Principle Findings: Systems level characterization of serum metabolome and lipidome of adult DF patients at early febrile, defervescence, and convalescent stages of DENV infection was performed using liquid chromatography- and gas chromatography-mass spectrometry. The tractability of following metabolite and lipid changes in a relatively large sample size (n = 44) across three prominent infection stages allowed the identification of critical physiologic changes that coincided with the different stages. Sixty differential metabolites were identified in our metabolomics analysis and the main metabolite classes were free fatty acids, acylcarnitines, phospholipids, and amino acids. Major perturbed metabolic pathways included fatty acid biosynthesis and β-oxidation, phospholipid catabolism, steroid hormone pathway, etc., suggesting the multifactorial nature of human host responses. Analysis of phospholipids and sphingolipids verified the temporal trends and revealed association with lymphocytes and platelets numbers. These metabolites were significantly perturbed during the early stages, and normalized to control levels at convalescent stage, suggesting their potential utility as prognostic markers. Conclusions/Significance: DENV infection causes temporally distinct serum metabolome and lipidome changes, and many of the differential metabolites are involved in acute inflammatory responses. Our global analyses revealed early anti-inflammatory responses working in concert to modulate early pro-inflammatory processes, thus preventing the host from development of pathologies by excessive or prolonged inflammation. This study is the first example of how an omic- approach can divulge the extensive, concurrent, and dynamic host responses elicited by DENV and offers plausible physiological insights to why DF is self limiting. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
42. Whole Cell-SELEX Aptamers for Highly Specific Fluorescence Molecular Imaging of Carcinomas In Vivo.
- Author
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Shi, Hui, Cui, Wensi, He, Xiaoxiao, Guo, Qiuping, Wang, Kemin, Ye, Xiaosheng, and Tang, Jinlu
- Subjects
- *
LUNG cancer diagnosis , *FLUORESCENCE , *CARCINOMA , *MOLECULAR probes , *CANCER cell culture , *CANCER cell differentiation , *FLOW cytometry - Abstract
Background: Carcinomas make up the majority of cancers. Their accurate and specific diagnoses are of great significance for the improvement of patients' curability. Methodology/Principal Findings: In this paper, we report an effectual example of the in vivo fluorescence molecular imaging of carcinomas with extremely high specificity based on whole cell-SELEX aptamers. Firstly, S6, an aptamer against A549 lung carcinoma cells, was adopted and labeled with Cy5 to serve as a molecular imaging probe. Flow cytometry assays revealed that Cy5-S6 could not only specifically label in vitro cultured A549 cells in buffer, but also successfully achieve the detection of ex vivo cultured target cells in serum. When applied to in vivo imaging, Cy5-S6 was demonstrated to possess high specificity in identifying A549 carcinoma through a systematic comparison investigation. Particularly, after Cy5-S6 was intravenously injected into nude mice which were simultaneously grafted with A549 lung carcinoma and Tca8113 tongue carcinoma, a much longer retention time of Cy5-S6 in A549 tumor was observed and a clear targeted cancer imaging result was presented. On this basis, to further promote the application to imaging other carcinomas, LS2 and ZY8, which are two aptamers selected by our group against Bel-7404 and SMMC-7721 liver carcinoma cells respectively, were tested in a similar way, both in vitro and in vivo. Results showed that these aptamers were even effective in differentiating liver carcinomas of different subtypes in the same body. Conclusions/Significance: This work might greatly advance the application of whole cell-SELEX aptamers to carcinomas-related in vivo researches. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
43. A Multi-Paradigm Modeling Framework to Simulate Dynamic Reciprocity in a Bioreactor.
- Author
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Kaul, Himanshu, Cui, Zhanfeng, and Ventikos, Yiannis
- Subjects
- *
BIOREACTORS , *TECHNOLOGICAL innovations , *CELL growth , *CELL populations , *CHEMOTAXIS , *CELL migration , *CELL proliferation , *BIOTECHNOLOGY - Abstract
Despite numerous technology advances, bioreactors are still mostly utilized as functional black-boxes where trial and error eventually leads to the desirable cellular outcome. Investigators have applied various computational approaches to understand the impact the internal dynamics of such devices has on overall cell growth, but such models cannot provide a comprehensive perspective regarding the system dynamics, due to limitations inherent to the underlying approaches. In this study, a novel multi-paradigm modeling platform capable of simulating the dynamic bidirectional relationship between cells and their microenvironment is presented. Designing the modeling platform entailed combining and coupling fully an agent-based modeling platform with a transport phenomena computational modeling framework. To demonstrate capability, the platform was used to study the impact of bioreactor parameters on the overall cell population behavior and vice versa. In order to achieve this, virtual bioreactors were constructed and seeded. The virtual cells, guided by a set of rules involving the simulated mass transport inside the bioreactor, as well as cell-related probabilistic parameters, were capable of displaying an array of behaviors such as proliferation, migration, chemotaxis and apoptosis. In this way the platform was shown to capture not only the impact of bioreactor transport processes on cellular behavior but also the influence that cellular activity wields on that very same local mass transport, thereby influencing overall cell growth. The platform was validated by simulating cellular chemotaxis in a virtual direct visualization chamber and comparing the simulation with its experimental analogue. The results presented in this paper are in agreement with published models of similar flavor. The modeling platform can be used as a concept selection tool to optimize bioreactor design specifications. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
44. Isolation of Rare Tumor Cells from Blood Cells with Buoyant Immuno-Microbubbles.
- Author
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Shi, Guixin, Cui, Wenjin, Benchimol, Michael, Liu, Yu-Tsueng, Mattrey, Robert F., Mukthavaram, Rajesh, Kesari, Santosh, Esener, Sadik C., and Simberg, Dmitri
- Subjects
- *
CELL separation , *CANCER cells , *BLOOD cells , *MICROBUBBLES , *CANCER prognosis , *CELL adhesion , *NANOTECHNOLOGY , *BIOMEDICAL engineering - Abstract
Circulating tumor cells (CTCs) are exfoliated at various stages of cancer, and could provide invaluable information for the diagnosis and prognosis of cancers. There is an urgent need for the development of cost-efficient and scalable technologies for rare CTC enrichment from blood. Here we report a novel method for isolation of rare tumor cells from excess of blood cells using gas-filled buoyant immuno-microbubbles (MBs). MBs were prepared by emulsification of perfluorocarbon gas in phospholipids and decorated with anti-epithelial cell adhesion molecule (EpCAM) antibody. EpCAM-targeted MBs efficiently (85%) and rapidly (within 15 minutes) bound to various epithelial tumor cells suspended in cell medium. EpCAM-targeted MBs efficiently (88%) isolated frequent tumor cells that were spiked at 100,000 cells/ml into plasma-depleted blood. Anti-EpCAM MBs efficiently (>77%) isolated rare mouse breast 4T1, human prostate PC-3 and pancreatic cancer BxPC-3 cells spiked into 1, 3 and 7 ml (respectively) of plasma-depleted blood. Using EpCAM targeted MBs CTCs from metastatic cancer patients were isolated, suggesting that this technique could be developed into a valuable clinical tool for isolation, enumeration and analysis of rare cells. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
45. Metal-Free Intramolecular Aziridination of Allylic Carbamates Mediated by Hypervalent Iodine Compounds.
- Author
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Qin-Hai Deng, Jing-Cui Wang, Zhen-Jiang Xu, Cong-Ying Zhou, and Chi-Ming Che
- Subjects
- *
AZIRIDINES , *CARBAMATES , *HYPERVALENCE (Theoretical chemistry) , *IODINE compounds , *AMINO alcohols , *CHEMISTRY - Abstract
An efficient and practical hypervalent iodine compound mediated metal-free intramolecular aziridination reaction of allylic carbamates was developed. Analytically pure aziridines were obtained in high yields by simple filtration of the reaction mixture. In situ ring opening of the aziridines provides an easy access to useful vicinal amino alcohol derivatives and diamine compounds. Up to 16% ee was obtained when chiral hypervalent iodine compound was used. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
46. Ab Initio Trajectory Surface-Hopping Study on Ultrafast Deactivation Process of Thiophene.
- Author
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Ganglong Cui and Weihai Fang
- Subjects
- *
THIOPHENES , *ORGANOSULFUR compounds , *ORGANIC cyclic compounds , *PHYSICAL & theoretical chemistry , *CHEMISTRY - Abstract
The ultrafast S1(¹ππ*)→ Sodeactivation process of thiophene in the gas phase has been simulated with the complete active space self-consistent field (CASSCF) based fewest switch surface hopping method. It was found that most of the calculated trajectories (~80%) decay to the ground state (So) with an averaged time constant of 65 ± 5 fs. This is in good agreement with the experimental value of about 80 fs. Two conical intersections were determined to be responsible for the ultrafast S1(¹ππ*)→ So internal conversion process. After thiophene is excited to the S1(¹ππ*) state in the Franck-Condon region, it quickly relaxes to the minimum of the S1(¹ππ*) state, then overcomes a small barrier near the conical intersection (CI(¹ππ*/¹πσ*)), and eventually arrives at the minimum of one C-S bond fission (S1(¹πσ*)). In the vicinity of this minimum, the conical intersection (CI(¹πσ*/So)) funnels the electron population to the ground state (So), completing the ultrafast S1(¹ππ*)→ So internal conversion process. This decay mechanism matches well with previous experimental and theoretical studies. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
47. Synthesis and characterization of a nickel-organic framework encapsulating hetero-chiral helical water chains in the 1-D channels.
- Author
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Wang, Cui-Juan, Ren, Ping-Di, Zhang, Zhi-Bin, Yi-Fang, and Wang, Yao-Yu
- Subjects
- *
CHEMISTRY , *COORDINATION polymers , *METAL clusters , *BIOMOLECULES , *HYDROGEN bonding - Abstract
A porous coordination polymer based on nickel(II) and rigid multitopic ligands, {[Ni(dpdapt)(BDC)(H2O)] · 3.5H2O}n (1) (dpdapt = N, N'-di(2-pyridyl)-2,4-diamino-6-phenyl-1,3,5-triazine), has been synthesized and characterized. Compound 1 crystallizes in the space group C2/m and possesses a 3-D open framework with 1-D rhombic hydrophilic channels, in which hetero-chiral helical water chains are located. Two 1-D water chains are further stabilized by hydrogen-bonding interactions with the host, inducing a 10-oxygen ring propagated along the channel. TGA, PXRD analyses, and magnetic properties have also been studied. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
48. High-Yield Synthesis of 1,3-Dimesityl-propane-1,3-dione: Isolation of Its Aluminum Complex as a Stable Intermediate.
- Author
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Zhang, Cui, Yang, Peiju, Yang, Yue, Huang, Xiaojuan, Yang, Xiao-Juan, and Wu, Biao
- Subjects
- *
ALUMINUM , *PROPANE , *FRIEDEL-Crafts reaction , *MESITYLENE , *CATALYSTS , *CHEMISTRY - Abstract
1,3-Dimesityl-propane-1,3-dione was synthesized in high yield, via a stable intermediate (its aluminum complex) from malonyl dichloride and mesitylene by Friedel-Crafts reaction using anhydrous aluminum chloride as catalyst. The intermediate aluminum complex was isolated and characterized by X-ray diffraction analysis, which decomposed upon reflux in concentrate hydrochloric acid to give the title compound. [ABSTRACT FROM AUTHOR]
- Published
- 2008
- Full Text
- View/download PDF
49. Preparation of red phosphor (Y, Gd)BO3:Eu by soft chemistry methods
- Author
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Cui, Xiangzhong, Zhuang, Weidong, Yu, Zhijian, Xia, Tian, Huang, Xiaowei, and Li, Hongwei
- Subjects
- *
FIRE , *CHEMISTRY , *FIREPLACES , *ARSON - Abstract
Abstract: The three soft chemistry methods were employed to prepare the red phosphor (Y, Gd)BO3:Eu, such as coprecipitation–combustion method, salt assisted combustion method and emulsion method. The main factors affecting particle size, particle distribution and luminescent properties of the product were investigated in detail, and as a result, the preparation processes were optimized. The phosphors were characterized by X-ray diffraction (XRD), scanning electronic microscope (SEM), transmission electronic microscope (TEM) and vacuum ultraviolet (VUV) spectra. Results reveal that phosphors with different morphology, small particle size and high luminescence intensity could be obtained by soft chemistry methods. The difference between the luminescence properties of phosphors in this work and commercial rare earth borate phosphor is discussed. The phosphor with grain shape and high luminescence intensity could be prepared by coprecipitation–combustion method, nanophosphor could be prepared by salt assisted combustion method, and spherical phosphor with a narrow size distribution could be obtained by using emulsion method. [Copyright &y& Elsevier]
- Published
- 2008
- Full Text
- View/download PDF
50. A facile synthesis and fungicidal activities of novel fluorine-containing pyrido[4,3-d]pyrimidin-4(3H)-ones
- Author
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Ren, Qingyun, Cui, Zeping, He, Hongwu, and Gu, Yucheng
- Subjects
- *
FLUORINE compounds , *SUBSTITUTION reactions , *CHEMICAL reactions , *CHEMISTRY - Abstract
Abstract: Sixteen novel 2-substituted-5,8,9-trimethyl-3-(4-fluoro-substituted)phenyl-thieno[3′,2′-5,6] pyrido[4,3-d]pyrimidin-4(3H)-ones 5a–5p were designed and have been successfully synthesized via tandem aza-Wittig and annulation reactions of the corresponding iminophosphorances 1, para-fluoro phenyl isocyanate, and substituted phenols or amines in 73–90% isolated yields. Their structures were clearly verified by IR, 1H NMR, EI-MS spectroscopy and elemental analysis, and in the case of compound 5a, analyzed by single-crystal X-ray diffraction further. The results of preliminary bioassay indicated that some compounds possess inhibition activities against Rhizoctonia solani and Botrytis cinereapers at a dosage of 50mg/L. [Copyright &y& Elsevier]
- Published
- 2007
- Full Text
- View/download PDF
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