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IV ÖZET Para konumunda birbirine bağlanmış benzen halkalarından oluşan poliparafenilen, PPP, katkılanmış türü ile iyi bir iletken olup yüksek mekanik dayanıklılığa, ısısal ve kimyasal kararlılığa sahiptir. PPP, hem katodik (n- katkılama) hem de anodik (p-katkılama) olarak katkılanabilmektedir. Bu nedenle de doldurulabilir pillerde hem anot hem de katot olarak kullanılabilmektedir. Bu polimerin monomeri olan benzenin susuz ortamda elektroyükseltgenmesi için yüksek potansiyel gerekmektedir. Elektroliz sırasında ortamda asit bulunması yükseltgenmeyi kolaylaştırmaktadır. Çözücü ve destek elektrolit de elde edilen filmlerin yapısını, iletkenliğini, elektrokimyasal davranışını ve yüzeye tutunmasını etkilemektedir. Ortamda su bulunması platin oksit oluşumu ile film eldesini engellemektedir. Bu çalışmada PPP, benzenin ve bifenilin florosülfonik asit (FHSO3) ve tetrabütilamonyum tetrafloraborat (TBABF4) veya tetrabütilamonyum perklorat (TBAP) içeren asetonitril veya metilenklorür çözeltisinde elektroyükseltgenmesi ile Pt elektrot üzerinde elde edilmiştir. Elektropolimerleşme için en uygun monomer ve asit derişimleri belirlenmiş ve elde edilen filmlerin kuru iletkenlikleri ölçülmüştür. Bu iletken filmlerin elektroaktif olup olmadıklarını incelemek amacı ile ferrosen, benzokinon ve potasyumferrosiyanürün elektrokimyasal davranışları dönüşümlü voltametri yöntemi ile incelenmiş ve davranışlar aynı maddelerin Pt elektrot ile ölçülen dönüşümlü elektrokimyasal davranışları ile karşılaştırılmıştır. Ayrıca elde edilen bu filmlerin İR spektrumları alınmış ve TG analizleri yapılmıştır. Bu çalışmalara ek olarak ortamdaki Ag+, Cu+ ve Pb^+ gibi iyonların elektrolizdeki katalizör etkisi incelenmiştir. ANAHTAR KELİMELER: Poliparafenilen, iletken polimerler, benzen, bifenil, metal iyonu katalizörü. ABSTRACT Poly(p-phenylene) (PPP) which is obtained from para-position-connected benzene rings attracted considerable amount of attention as a conducting polymer due to its high mechanical strength, thermal and chemical stability in air. It can be doped with anions (p-doping) as well as with cations (n- doping). This way it can function as an anode or a cathode material in rechargeable batteries. The electrooxidation of its monomer, benzene, requires very high applied potential in nonaqueous media. The presence of acids in electrolysis solution causes relatively easy oxidation. The type of the solvent and supporting electrolyte used during electrolysis influence the morphology, conductivity, electrochemical behavior and adhesion of the resulting films. The presence of water leads to the formation of platinum oxide film which inhibits the polymer formation. In this study, PPP was obtained by the electrooxidation of benzene and biphenyl in acetonitrile or methylenechloride containing fluorosulphonic acid and tetrafluoroammonium tetrafluoroborate (TBABF4) or tetrabutyl- ammonium perchlorate (TBAP) salts on the Pt electrode. The optimum electropolymerization conditions with respect to the monomer and acid concentrations were established and dry conductivity values were measured. In order to understand whether these films are electroactive or not, the electrochemical behaviors of ferrocene, benzoquinone and potassium- ferrocynide were investigated using cyclic voltametry and compared with measured by using Pt electrode. The IR spectra and TG analysis of these films were also examined. In addition to these studies, the effect of catalysts on the electropolymerization such as Ag+, Cu+ and Pb^+ ions in the medium was also examined. KEY WORDS: Polyparaphenylene, conducting polymers, benzene, biphenyl, metal ion catalysis 76

ÖZET nükleer magnetik rezonans spektroskopi ve demir çöktürme metodlari ile türk yiyeceklerinde fitat tayini ERSOZ, Arzu Yüksek Lisans Tezi, Kimya Bölümü Tez Yöneticisi: Prof. Dr. Namık K. Araş Eylül, 1988 İkili porsiyon tekniği ile sosyal-ekonomik durumu farklı iki değişik guruptan, birbirini takip eden üç gün boyunca diyet örnekleri toplandı. Numuneler parçalandı ve kurutuldu. Fitat miktarları demir çöktürme ve nükleer magnetik rezonans (NMR) teknikleri ile ölçüldü. Pişirme, ıslatma, çimlendirme gibi prosesler fitatın hidrolizine neden olur, bu yüzden 31P-NMR çalışmalarında fosfor pikleri daha düşük inositol verecek şekilde bozulur. Fitatın katı numuneden alınabilme basamağı optimize edildi ve 40 mi 0.5% HC1, 2.0 g numune için en iyi ayırıcı olarak kabul edildi. viDiyet numunelerinin yanı sıra, buğday ve pirinç analizleri de yapıldı. Ortanın üzerinde geliri olan gurubu kapsayan ODTÜ gurubunun fitat miktarı 0.3-2.9 g/kg arasında değişmektedir ve ortalama miktarda 1.55 g/kg'dır. Ortalama buğday miktarı 7.5 g/kg ve pirinç miktarı 1.51 g/kg' dır. Diğer ülkelerin toplam diyetlerindeki fitat miktar analizleri henüz bitmiş değil ve yayınlarda diyetlerdeki fitat derişimleri ile ilgili bilgi çok az. Anahtar kelimeler: Fitat, fitik asit, inositol, 3 İP Fourier dönüşüm nükleer magnetik rezonans, demir çöktürme. vii ABSTRACT DETERMINATION OF PHYTATE IN TURKISH DIETS BY SiP-NMR AND IRON-PRECIPITATION METHOD ERSÖZ, Arzu M. S. in Chemistry Supervisor: Prof. Dr. Namık K. Aras September, 1988 We have collected diet samples three consequtive days from two different socio-economic population group by applying duplicate portion technique. They were homogenized and freeze dried. Their phytate contents were determined by iron-precipitation and 31P-NMR methods. We have also analyzed phytate content of wheat and rice samples. Some process such as cooking, soaking, fermentation, germination cause the hydrolysis of phytate, so that phosphorus peaks are broken in 31P-NMR studies, giving rice lower inositol phosphates. Extraction of phytate from the solid samples was optimized, 40 ml 0.5% HC1 was found best extractant up to 2.0g diet samples. The phytate content of METU samples which corresponds to middle-higher income population group, varies between 0.3-2.9 g/kg ivdried diet with an average 1.55 g/kg. The average phytate content of wheat and rice were found to be 6.25 g/kg and 1.51 g/kg, respectively. Phytate content of total diet of other countries are not well established and very few results are givin in literature. Keywords: Phytate, phytic acid, inositol, 3 IP Fourier Transform Nuclear Magnetic Resonance.iron-precipitation. 69

WOS: 000470572900001, In this study, we have focused our attention on developing a novel immunoadsorbent while combining cryogel-based specific sorbents with immunoaffinity (anti-LDL antibody). A novel method has been developed by our group abbreviated as ANADOLUCA in which biomolecules could be converted into protein-like fluorescent nanoparticles through ruthenium-mediated polymerization and crosslinking reactions. Herein, anti-LDL antibody nanoparticles that have an average particle size as 50 nm with a narrow size distribution have been prepared via ANADOLUCA method. These nanoparticles were then embedded in the polymeric structure to give immunospecificity to the cryogens during the cryo-polymerization process under semi-frozen conditions. After characterization of the materials by scanning electron microscopy and swelling tests and optimization of depletion, conditions have been investigated by varying affecting parameters like initial LDL concentration and pH of the solution. Finally, the cryogel column developed has been conducted to fast protein liquid chromatography system as a stationary phase to evaluate its chromatographic performance not only from aqueous LDL solution and but also from human serum in a single step separation process. As a result, an efficient LDL depletion performance has been achieved for the cryogel column in short treatment time. Therefore, the cryogel column might be classified as potential cost-friendly and efficient candidate for immunoaffinity depletion of LDL molecules from hypercholesterolemic human serum., Anadolu University, 'The Scientific Research Project - BAP [BAP-1106 F122], This work was supported by the Anadolu University, 'The Scientific Research Project - BAP [BAP-1106 F122].

In the present study, N-Acetylneuraminic acid (NANA)-imprinted poly(2-hydroxyethyl methacrylate-N-methacryloyl-(L)-histidin-Cu(II)) p(HEMA-(MAH) 2 -Cu(II)) has been synthesized by radical polymerization for site recognition of ceruloplasmin. Prepared imprinted cryogel has been characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). When the binding capacity of NANA-imprinted cryogel (MIP) was compared with non-imprinted cryogel (NIP), it was found that MIP has higher adsorption capacity. The maximum amount of binding NANA has found as 83.20 mgg -1 at pH 7.0 with flow rate of 1 mLmin -1 at 25°C. Selectivity experiments for MIP and NIP cryogel have been carried out via the NANA/D-mannose and ceruloplasmin/immunoglobulin G (IgG) pair separately and the relative selectivity coefficient (k’) has found as 77.46 and 17.97, respectively. Applicable of the MIP in human serum has been studied and resulted successfully

WOS: 000440300100012, This study demonstrates the preparation and characterization of a novel ion imprinted cryogel which exhibits high affinity and selectivity towards Ce(III) ions in aqueous solutions and bastnasite ore samples. 2-Hydroxyethyl methacrylate (HEMA) and N-methacryloylamido antipyrine (MAAP) were used as functional monomers for the preparation of Ce(III) imprinted cryogel. The effects of various factors such as initial Ce(III) concentration, flow rate, pH, interaction time and ionic strength on the Ce(III) binding to the prepared ion imprinted cryogels were also studied. The binding equilibrium for Ce(III) is obtained in 30 min at the flow rate of 0.5 mL/min. The maximum binding capacity of the prepared ion imprinted cryogel towards Ce(III) is obtained as 36.58 mg/g at optimum conditions. The selectivity of the prepared ion imprinted cryogel towards Ce(III) in the presence of other possible interfering lanthanide ions such as La(III) and Nd(III) were also performed. The obtained results showed that the prepared ion imprinted cryogel exhibits high selectivity and sensitivity towards Ce(III) ions. The limit of detection (LOD) was found as 50 mu g/L.

WOS: 000404704300041, PubMed ID: 28629026, A multistate biomemory device consisting of cytochrome c (Cyt-c) photosensitively cross-linked by MACysRu(bipyr)(2)-MACys hapten molecules, which have memory effect through a charge transfer mechanism, has been developed. In this study, it has suggested a highly resolute surface-confined switch composed a signal-enhanced electro-active protein (Cyt-c) co-polymerized on the gold substrates that can be controlled by redox property through Ruthenium based cysteine monomer hapten, MACys-Ru(bipyr)(2)-MACys as an ANADOLUCA photosensitive cross-linker. The photosensitive cross-linking of the Cyt-c protein on the gold surface topography has been determined by the scanning electron microscopy (SEM). Two state memory functions, writing and erasing of the developed biomemory device, have been investigated by the chronoamperometry (CA) and open-circuit potential amperometry (OCPA). The polymeric proteinous memory device, p(MACys-Ru(bipyr)(2)-MACys-coCyt-c) layer, on the gold electrode is stable and repeatable up to with 10(4) times continuous cycle

WOS: 000383930900027, PubMed ID: 27612708, In this study, a simple, rapid and sensitive method based on novel molecular imprinted polymeric sensor has been developed and validated for the determination of prostate cancer metabolite biomarker. The molecularly imprinted polymer (MIP) has been synthesized by emulsion polymerization, using sarcosine as template molecule, methacryloylamido histidine (MAH) as functional monomer and ethylene glycol dimethacrylate (EDMA) as cross-linking agent. The performance of the developed sarcosine sensor has been evaluated, and the results have indicated that a sensitive potentiometric sensor has been fabricated. The sarcosine sensor has showed high-selectivity, shorter response time (5.5 months)

WOS: 000481724000023, This study describes the development of cryogel columns prepared by ATP having ruthenium hapten cross-linker based photosensitive approach "ANADOLUCA" for the biotinylation of Crimean Congo Hemorrhagic Fever (CCHF) primers through 5' end as a first. In this process, which does not require ATP ligand, CCHF recognition has been performed using stable and quantum dots (QDs) based avidity nanoparticles cross-linked with streptavidin. p(HEMA-co-DNA ligase) photosensitive copolymerized cryogel column as a biotinylated continuous system have prepared using ATP ligand having photosensitive ruthenium chelate based hapten cross-linker and characterized. Then, the biotinylated efficiency of the prepared column has performed through continuous biotin binding without any need of ATP ligand having to Crimean congo hemorrhagic fever primer (CCHF). The efficiency of the biotinylated CCHF primer has investigated by polymerase chain reaction (PCR). The characteristic of a sustainable biotinylating column synthesized from the same ligand that we normally develop using DNA ligase while working with ATP in the DNA ligase biotinylation process is that it can biotinize without ATP. In addition, virus-identifying ssDNA was observed as fluorescence after avidity interaction with the QD combination for viral identification after biotinylation. Then, the fluorescence intensity changes through the hybridization of 3'-ssDNA complements have determined., Bionkit Limited Company; Scientific and Technological Research Council of Turkey (TUBITAK) [1130016], This study has been partially supported by Bionkit Limited Company and Scientific and Technological Research Council of Turkey (TUBITAK) (Project number 1130016 within the scope of Tubitak 1511 Priority Areas Research Technology Development and Innovation Project Support Program).

Yüksek kan kaybına neden olan cerrahi operasyonlar, kan transfüzyonu gerektiren acil durumlar ve transfüzyon sırasında ortaya çıkan hemolitik reaksiyonlar, eşleştirme işleminin yol açtığı zaman kaybı, viral hastalıkların bulaşma riski sebebiyle oksijen taşıyan kan ikamelerine olan gereksinim, son dünya savaşı itibari ile fazlasıyla artmıştır. Salgın hastalıklar, doğal ve insan kaynaklı felaketler nedeniyle ortaya çıkan kan ihtiyacını karşılama amacıyla kullanılması hedeflenen oksijen taşıyan kan ikameleri ile ilgili çalışmalar, son yıllarda yeni bir biyoteknoloji araştırma alanı oluşturmuştur. Bu çalışmada ise oksijen taşıyan kan ikamesi olarak kullanılması amaçlanan hemoglobin kapsüllenmiş lipozom sistemleri geliştirilmiştir. Hemoglobinin zamanla oksitlenip methemoglobine (met-Hb) dönüşmesini engellemesi amacıyla antioksidan olarak kullanılan seryum oksit nanopartiküller de (nanoseria) lipozomun içine enkapsüle edilmiştir. Karakterizasyon işlemi Taramalı Elektron Mikroskobu (SEM), boyut analizi için ZetaSizer ve kapsülleme veriminin belirlenmesi için UV-vis spektroskopisi kullanılarak gerçekleştirilmiştir. Sonuç olarak, hemoglobin bazlı oksijen taşıyan kan ikamesi, toksik ve antijenik olmayan, stabilitesini uzun süre koruyabilen nano-taşıyıcı sistem olarak geliştirilmiştir. Çalışmanın ikinci aşmasında ise likit kristale dayalı, kan grubu tayininde alternatif olarak kullanılabilecek evet/hayır prensibi doğrultusunda, yeni nesil ve hızlı teşhise yönelik bir yöntem geliştirilmiştir. Çalışmada öncelikle (4'-siyano-[1,1'-bifenil]-4-yl) boronik asit sentezlenip, NMR ile karakterize edilmiştir. Boronik asit, glikoza olan yüksek afinitesi sayesinde, anti-A ve anti-B antikorlarının yapısında bulunan glikoz ile etkileştirilmiştir. Nematik özellik kazandırılan antikorlar kan antijeni ile etkileştirildiğinde likit kristalin yönleniminde oluşan farklılık polarize ışık mikroskobu altında görüntülenmiştir. Anahtar Sözcükler: Lipozom, Hemoglobin, Nanoseria, met-Hb, Likit kristal Surgical operations leading to high blood loss, emergency cases requiring blood transfusion and hemolytic reactions during transfusion, loss of time caused by pairing process, the need for oxygen-carrier blood substitute due to the risk of transmission of viral diseases have increased greatly as of the last world war. Studies on blood substitution with oxygen-carrying, intended to be used for the purpose of meeting the blood need for epidemics, natural and man-made destructions, have recently formed another biotechnology research area. In this study, hemoglobin encapsulated liposome which is intended to be used as oxygen-carrying blood substitution has been developed. Cerium oxide nanoparticles (nanoceria), which are used as antioxidants, have encapsulated into the liposome in order to prevent hemoglobin being oxidized over time and converted to methemoglobin (metHb). Characterization process has performed by using Scanning Electron Microscopy (SEM), Zetasizer for size analysis and UV-Vis spectroscopy to determine the encapsulation efficiency. As a result, hemoglobin-based oxygen-carrying blood substitution, non-toxic and non-antigenic, has been developed as a nano-carrier system that can maintain its stability for a long time. In the second stage of the study, a new generation and rapid diagnosis method was developed based on the liquid crystal and the yes / no principle which can be used as an alternative in blood group determination. (4'-cyano- [1,1'-biphenyl] -4-yl) boronic acid was synthesized and characterized by NMR. Due to the high affinity of boronic acid to glucose, boronic acid is interacted with glucose in the structure of antibodies. When antibodies with nematic properties were interacted by blood antigen, the difference in liquid crystal orientation was observed under polarized light microscope.Keywords: Liposome, Hemoglobin, Nanoceria, metHb, Liquid crystal 86

Yapılan bu çalışmanın amacı, klorofil sentezinin ilk basamağı olan Magnezyum protoporfirini, protoporfirin IX ve Mg+2 varlığında ve magnezyum şelataz enzimi katalizliğinde laboratuvar koşulları çerçevesinde sentezlemektir. Bu doğrultuda yeşil bitki yapraklarından magnezyum şelataz enzimi izole edilerek saflaştırılmıştır. Magzeyum şelataz enzimini saflaştırmak için boya afinite kromatografi tekniği ve sibakron mavisi boya olarak kullanıştır. Sentezlenen boya afinite kolonunun karakterizasyonu, kızılötesi spektroskopi (IR), şişme testi ve taramalı elektron mikroskobu (SEM) görüntüsü gibi parametreler kullanılarak gerçekleştirilmiştir. Saflaştırılan magnezyum şelataz enzimi ve magnezyum içeren protein karışımı, Magnezyum protoporfirin sentezi için destek materyal olarak, ANADOLUCA metodu ile Poli (HEMA-ko-Magzenyum-şelataz) kolonuna immobilize edilmiştir. Sentezlenen kriyojel kolonların karakterizasyonu için şişme testleri yapılmış ve IR ve SEM görüntüleri alınmıştır. Protoporfirin IX ve Mg+2 iyonunu içeren çözeltisinin kolondan geçirilmesiyle sentezlenen Magnezyum protoporfirin fluorimetrik yöntemle belirlenmiştir. The purpose of this study is to synthesize Protoporphyrin IX, Mg2+ in the laboratory conditions of the resultant Magnesium Porphyrin which is the first step of chlorophyll synthesis in the catalysis of magnesium chelatase enzyme. Accordingly, enzymes of magnesium chelatase have been extracted from the green plant leaves and purification procedures have been applied. Dye affinity chromatography has been used to purify the magnesium chelatase enzyme and cibacron blue has used as a dye. The characterization of the synthesized dye affinity column has performed by using parameters such as infrared spectroscopy (IR), swelling tests and scanning electron microscopy (SEM) images. Subsequently, a protein mixture containing poly (HEMA-co-magnesium chelatase) column and magnesium chelatase enzyme according to the Aminoacid (monomer) Decorated and Light Underpinning Conjugation Approach (ANADOLUCA) method has immobilized to provide support material for Magnesium Porphyrin synthesis. Swelling tests have performed for the characterization of the synthesized column material, IR and SEM images have been taken. Magnesium Porphyrin synthesized by passing Protoporphyrin IX and Mg2+ solution through the column has been determined by the fluorimetric method. 59

WOS: 000470939700021, In this study, binanoenzyme (BNE) was synthesized using FateDH and Cyt-C in same bionano-interface; by formation of formate dehydrogenase-co-cytochrome C by using ANADOLUCA method. The synthesized novel binanoenzyme was embedded into HEMA based cryogel column and the conversion of CO2 to formic acid was investigated by using this cryogel column. To maintain the conversion, CO2 saturated water was passed through the cryogel column and the converted formic acid was analyzed using HPLC. The parameters which has an effect on conversion such as pH, sample volume and CO2 concentration were analyzed. The results showed that the conversion process was succeeded effectively., Anadolu University [BAP-1605F431], This study was supported by Anadolu University, 'The Scientific Research Project-BAP' (BAP-1605F431).

WOS: 000474571400010, PubMed ID: 30963369, This study demonstrates the preparation of photosensitively orientated and crosslinked proteinous polymeric shell having quantum dot based nanocrystals through Amino acid Decorated and Light Underpinning Conjugation Approach (ANADOLUCA). ANADOLUCA is based on photo-electron transfer method and uses these decorated nanocrystals for specifically and effectively recognition and detection of Immunoglobulin M in the aqueous environment. The conjugation method effectively provides an orientation of affinity pairs on the surface of quantum dots nanocrystals. This photosensitive ruthenium-based amino acid monomer is a synthetic and inexpensive material for the preparation of bioconjugates. The nanocrystals give advantages for using a wide pH and temperature range. The construction and preparation method is applicable to silica materials, superparamagnetic particles, quantum dots, carbon nanotubes, Ag/Au nanoparticles, Au surfaces, and polymeric materials. This prepared proteinous polymeric shell decorated nanocrystals are of great potential in applications in life sciences and can be used in infection case studies or allergy symptoms.

WOS: 000382372100015, For biomarker discovery, simultaneous removal of albumin and immunoglobulin G (IgG) become more important for both concentrating other species including potential biomarkers and getting rid of their masking effect. In this study, we have proposed a covalent and photosensitive cross-linking conjugation of the biomolecules on nanostructures for the depletion of target proteins from aqueous solution and serum. The effect of concentration, pH, temperature and ionic strength on the adsorption of twin affinity nanotraps based on Cibacron Blue F3GA and Protein A were investigated. The efficiency of albumin and IgG depletion from human serum was performed using sodium dodecylsulfate-polyacrylamide gel electrophoresis.

WOS: 000374638400001, The aim of this study is to prepare supermacroporous cryogel with embedded ion imprinted polymer (IIP) particles which can be used for the selective removal of Tl(I) from aqueous solution. In the first step, Tl(I) has complexed with chitosan and crosslinked with ephychlorohydrin. In the second step, the supermacroporous poly(hydroxyethyl methacrylate) (PHEMA) cryogel with embedded IIP particles [PHEMA/MIP composite cryogel] which have been produced by free radical polymerization initiated by N,N,N',N'-tetramethylene diamine (TEMED) and ammonium persulfate (APS) pair in ice bath. The template, Tl(I), has been removed from the polymer using 0.1 M EDTA solution. The cryogel is characterized by swelling test, scanning electron microscopy, and Fourier transform infrared spectroscopy. The effective parameters on Tl(I) adsorption process have evaluated by investigating the dependency of the adsorption capacity on flow-rate, Tl(I) concentration and contact time. The maximum adsorption capacity of Tl(I) ions has been found to be 1.55 mu gg(-1). The selectivity of the IIP biopolymer cryogel has investigated by competitive adsorption of Cd(II), Cu(II), K(I), and Fe(II). The relative selectivity coefficients of IIP biopolymer cryogel are 3.98 and 62.5 for Cd(II) and Cu(II), respectively. The IIP biopolymer cryogel could be used many times without any significant decrease in the adsorption capacity. As a conclusion it can be said that the IIP biopolymer cryogel could be used for specific separation of Tl(I) ions from nuclear, industrial, and environmental waste.

WOS: 000372031000005, Potentimetric sensors based on molecularly imprinted polymers are reported for the selective determination of clenbuterol. The work involved the modification of multiwalled carbon nanotubes, the modification of chitosan with diethylene triamine pentaacetic acid, the synthesis of the clenbuterol-imprinted polymer, and the preparation of potentiometric sensors using the clenbuterol-imprinted polymer. The effect of pH, response time, lifetime, selectivity, reusability, reproducibility, accuracy, and stability of the sensors were characterized. The optimum pH was 6.0 for the prepared poly(vinyl chloride) membrane and carbon paste electrodes. The limit of detection of the poly(vinyl chloride) membrane electrode was 0.49x10(-8)mM, with the linear dynamic range from 1.0x10(-5) to 1.0x10(-9)mM. The response time for the membrane electrode was 2min and the operational lifetime was 23 weeks. For the carbon paste electrode, the limit of detection was 0.91x10(-8)mM, the linear dynamic range was from 1.0x10(-4) to 1.0x10(-9)mM, the response time was 1min, and the operational lifetime was 24 weeks., Eskisehir Osmangazi University [2013-16], This project was financially supported by Eskisehir Osmangazi University. The Project number is: 2013-16.

WOS: 000442710600009, Carbonic anhydrase (carbonic dehydratase) (CA) is a metalloenzyme that contains zinc (Zn2+) ion in its active site. CA catalyzes the reversible conversion of carbon dioxide and water to bicarbonate and protons. Zn2+ ions, which are present in the active site of the enzyme, interact with the substrate molecules directly and cause catalytic effect. In this study, a nano-enzyme system was designed in aqueous solutions at room temperature and under nitrogen atmosphere to use the CA enzyme without any pre-treatment and deformation in its structure. The novel concept ANADOLUCA (AmiNo Acid (monomer) Decorated and Light Underpinning Conjugation Approach) was used for this process, nano CA enzyme of size 93 nm was synthesized. The activity of the synthesized nano CA was measured following the change in absorbance during the conversion of 4-nitrophenylacetate (NPA) to 4-nitrophenylate ion at 348 nm for a period of 10 mM at 25 degrees C compared with free CA enzyme. Km and Vmax values for nano CA enzyme were found to be 0.442 mM and 1.6 x 10(-3) mM min(-1) respectively, whereas Km and Vmax values for free CA were found to be 0.471 mM and 1.5 x 10(-3) mM min(-1) respectively. In addition to these, the Zn2+ ion present in the active site of the nano CA enzyme was replaced by rodium metal. This nanorodium-substituted CA has been investigated as a new reductase enzyme for the stereoselective hydrogenation of olefins. Then, the Zn2+ ion in the active site of the nano CA enzyme was replaced with manganese metal to enhance the enzyme structure, thereby gaining characteristics of peroxidase. This newly synthesized nano manganese-substituted CA enzyme was investigated for its role as a peroxidase, which could be an alternative for hydrogen peroxidases., Turkish Scientific and Technical Research Council-TUBITAK [TBAG-112T824], This study was supported by Turkish Scientific and Technical Research Council-TUBITAK (Project number: TBAG-112T824). We thank Medicinal Plant, Drug and Scientific Research Center, Anadolu University (AUBIBAM), for 1H-NMR analyses.

WOS: 000423765300006, A large-area, all-flexible, microwaveable polydimethoxysilane microfluidic reactor was fabricated by using a 3D printing system. The sacrificial microchannels were printed on polydimethoxysilane substrates by a direct ink writing method using water-soluble Pluronic F-127 ink and then encapsulated between polydimethoxysilane layers. The structure of micron-sized channels was analyzed by optical and electron microscopy techniques. The fabricated flexible microfluidic reactors were utilized for the acetylation of different amines under microwave irradiation to obtain acetamides in shorter reaction times and good yields by flow organic synthesis.

WOS: 000432038400025, The present study describes an easy and efficient procedure for the purification of horseradish peroxidase from horseradish roots. For this purpose, supermacroporous cryogels having Concanavalin A were prepared by photosensitive cross-linking polymerization. Horseradish peroxidase binding and elution from the prepared cryogels were carried out changing various parameters such as initial peroxidase concentration and pH. The best binding performance was obtained at pH 7.0. The maximum horseradish peroxidase binding of the cryogels was found to be 3.85 mg g(-1) cryogel. Horseradish peroxidase purification from crude extract resulted in 115.1-fold. SDS-PAGE analysis and circular dichroism measurements indicated that the horseradish peroxidase purification from horseradish roots was successfully carried out., Scientific and Technological Research Council of Turkey (TUBITAK) [1130019], The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) (Project Number: 1130019).

WOS: 000412610100012, PubMed ID: 28917810, In this study, a biomemory device, consisting of hemoglobin (Hb) cross-linked by MACys-Ru(bipyr)(2)-MACys) photosensitive monomer cross-linkers, which have memory effect through both Ru3+/2+ in hapten monomer and Fe3+/2+ in redox active center of Hb through multi-charge transfer mechanism, has been improved. Cyclic voltammetry (CV) has been used to determine the redox property of the Hb cross-linked MACys-Ru(bipyr)(2)-MACys) hapten. Three memory functions, writing, reading and erasing of the fabricated biomemory device, have been accomplished by chronoamperometry (CA) and open-circuit potential amperometry (OCPA). The reliability and repeatability of the biodevice consisting of the p(Hb-co-MACys-Ru(bipyr)(2)-MACys) sextet state bio-memory layer have been analysed. The Hb film based biodevice on gold electrodes has shown >= 2 months the retention time and switched until 10(6) times continuous cycling without degradation in efficiency. Other hand, the topography of p(Hb-co-MACys-Ru(bipyr)(2)-MACys) layer on the gold surface has investigated by scanning electron microscopy (SEM) and EDX data.

WOS: 000428134700001, In this study, Ce3+-based cryogel with methacryloyl antipyrine (MAAP) and 2-hydroxyethyl methacrylic acid (HEMA) [p(HEMA-MAAP-Ce3+] was prepared. MAAP-Ce3+ complex was characterized by UV-near infrared and energy-dispersive X-ray spectroscopy. Cryogel was characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, and swelling test. Pore size of the cryogel was found to be about 30-50 mu m. The effects of flow rate, pH, temperature, and initial enzyme concentration have been investigated. Maximum adsorption capacity was found to be 57.84 mg g(-1) cryogel at pH 6.0. After seven times of adsorption-desorption cycles of same cryogel, it was observed that there is negligible decrease in the adsorption capacity. [GRAPHICS] .

3rd International Turkish Congress on Molecular Spectroscopy (TURCMOS) -- AUG 26-29, 2017 -- Bodrum, TURKEY, WOS: 000449132500025, The present work demonstrates the selective recognition of myoglobin in human serum in the presence of various competitive proteins such as lysozyme, cytochrome c and hemoglobin by using molecularly imprinted affinity cryogels. The imprinted affinity cryogels towards myoglobin were prepared by using cryopolymerization technique. For this purpose, N-methacryloylamido antipyrine (MAAP)-Ce(III) and myoglobin were used as the complex functional monomer and template molecule, respectively. (MAAP)-Ce(III) complex functional in the imprinted cryogel provided a luminescence feature to the cryogel and the detection of the myoglobin was successfully achieved by UV-VIS-Near Infrared (UV-VIS-NIR) Spectroscopy. The effects of some variables such as initial myoglobin concentration, temperature, pH and ionic strength on myoglobin binding to the prepared cryogels were studied. The myoglobin binding capacity of the prepared cryogels was determined to be 68 mgg(-1). Myoglobin binding to the cryogels in the presence of competitive proteins lysozyme, cytochrome c and hemoglobin were also performed. The imprinted cryogels exhibited high selectivity towards myoglobin and the obtained binding order under competitive conditions was myoglobin > lysozyme > cytochrome c > hemoglobin

WOS: 000355054400015, This study describes the selective recognition of Nd(III) in aqueous, solutions in the presence of various lanthanide ions by molecularly imprinted polymers. Molecularly imprinted polymers (MIPs) were prepared by suspension polymerization. N-methacryloylamido folic acid (MAFol) was used as the functional monomer. The effects of different variables such as pH, time, and initial Nd(III) concentration on binding characteristics were investigated. Binding, equilibrium time was achieved in 30 min. The maximum binding capacity was found to be 14.6 mg Nd(III) g(-1) polymer. Binding studies of W(III) in the presence of Ce(III), La(III), and Eu(III),ions were also carried out using Nd(III)-imprinted polymers. The imprinted polymers displayed high selectivity toward Nd(III) ions. The obtained binding order under competitive conditions was Nd(III) > La(III) > Ce(III) > Eu(III).

WOS: 000358744700018, This paper describes a dimethoate imprinted polymeric potentiometric sensor for the determination of dimethoate that was designed by dispersing the dimethoate-imprinted polymer in dibutyl phthalate plasticizer and then embedded in polyvinyl chloride matrix. The method is based on molecularly imprinted polymer (MIP), which can be synthesized by using dimethoate as a template molecule, chitosan-Cd(II), and 3-mercaptopropyl-trimethoxysilane as a functional monomer and TEOS, and ephychlorohydrin as a cross-linking agent. We have studied a method for the selective binding behavior of dimethoate compounds on the surface of imprinted polymers which is prepared using ligand-exchange (LE) (chitosan-Cd(II)) monomer. The MIP-LE-1 and MIP-LE-2 based membranes have shown a detection limit of 4.3x10(-6) mmolL(-1) and 3.5.10(-5) mmolL(-1), respectively. The sensor has exhibited a response time of 35min for MIP-LE-1 and 120min for MIP-LE-2. They are stable for more than three months.

WOS: 000350247900015, PubMed ID: 25376531, As one of the most important components copying DNA molecules in the PCR system, Taq DNA polymerase has a high processivity, however, lower persistence when compared to other polymerases. Studies for the enhancement of stability of Taq DNA polymerase is of great importance. The present study describes the integration of PCR application of cross-linked Taq DNA polymerase enzyme in a nanochamber using a ruthenium based MATyr-Ru-(bipyr)(2))-MATyr monomer hapten prepared by photosensitive microemulsion polymerization technique. The conjugation and cross-linking have achieved using our previously invented Aminoacid (monomer) Decorated and Light Underpining Conjugation Approach (ANADOLUCA) method. Microemulsion polymerization media has prepared by dispersing PVA in deionized water. The nano enzyme could be easily prepared at room temperature, in daylight and under nitrogen atmosphere using ruthenium based photosensitive cross-linking agents. The nano copy machine particles (nano Taq DNA polymerase) are very stable against more acidic or more basic conditions, high temperatures and could be reusable in PCR analysis for many times without any deformation in their structures

WOS: 000416545300078, Both recovery and effective removing of radioactive thallium and iodine are important subject to protect human health and the environment. In this study, we have studied synergistic and cost-effective cryogel based sorbents by combining the high selectivity adsorption ability of MIP and the high porosity of cryogels. For thallium-imprinted cryogel; N-methacryloyl-(l)-cysteine (MAC) has synthesized, MAC has complexed with thallium ion and polymer cryogel has been prepared. For iodine-imprinted cryogel; MAC-thallium has complexed with iodine; and polymer cryogel has been prepared. Then, the adsorption, desorption, selectivity and reusability experiments have been carried out., ESOGU [2014-492], This work was supported by ESOGU (Project Number is 2014-492).

WOS: 000329200400002, In this study, we have proposed a novel organophosphate mimic surface molecular imprinted polymer for selective binding of the nerve agent by chitosan-Cd(II) as a new metal-chelating monomer via metal coordination-chelation interactions and dimethoate templates. We have improved a method for the selective binding behavior of dimethoate compounds on the surface of molecular imprinted polymer, which was prepared using ligand-exchange (chitosan-Cd(II)) monomers. The influence of ligand exchange imprinting on the creation of recognition sites toward dimethoate has determined application of adsorption isotherms. The effect of initial concentration of dimethoate, adsorption time, and imprinting efficiency on the adsorption selectivity for molecular imprinted polymer-ligand exchange has been investigated. The number of accessible sites (Qmax), relative selectivity coefficients (k), and binding abilities have also been evaluated. Then, molecular imprinted polymer-ligand exchange was treated with formaldehyde to remove dimethoate templates.

WOS: 000328593100001, PubMed ID: 23928053, In this study, we aimed to develop an alternative matrix able to deplete the albumin (Alb) and immunoglobulin G (IgG) from blood plasma simultaneously to prepare plasma samples for large-scale applications of blood-related proteomics. As a first step, nano-protein A nanoparticles (nanoProA) were prepared and characterized. Subsequently, cibacron blue F3GA (CB) was immobilized onto the nanoProA's to enhance their specific affinity for Alb molecules. Finally, both nanoparticles, specifically, nanoProA and CB-nanoProA, were separately embedded into cryogel structures to combine advantages of the nanoparticles with those of the cryogels. The protein adsorption was optimized using aqueous Alb and IgG solutions separately. Subsequently, competitive protein adsorption was performed using a protein mixture prepared with Alb and IgG adhering to their plasma protein ratios. Because of the CB-immobilization, the Alb depletion performance of the cryogels increased whereas the IgG depleting performance decreased. Using the nanoProA, embedded cryogel removed 99.3% of the IgG, while using the CB-nanoProA embedded cryogel removed 97.5% of the Alb content. The simultaneous depletion performances of the cryogels for Alb and IgG were characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In this study, the monolithic cryogel-based adsorbents were classified as an alternative matrix to prepare plasma samples for proteomics applications at the preparative scale

WOS: 000326317200005, In this study, a novel quartz crystal microbalance (QCM) based on the modification of paraoxonimprinted polymer onto a QCM with a high selectivity and sensitivity has been developed for the determination of paraoxon. The QCM sensor has characterized using AFM and ellipsometer. The performance of the QCM sensor has evaluated and presented good reproducibility, shorter response time (20 min), wider linear range (0.02-10 mu M) and low detection limit (0.02 mu M). The results have shown that the selectivity of QCM sensor has found as being very high in the presence of parathion which is similar in structure with paraoxon., Eskisehir Osmangazi University, Commission of Scientific Research Projects [200619004], This work has been supported by Eskisehir Osmangazi University, Commission of Scientific Research Projects (Project No: 200619004).

WOS: 000323591100008, PubMed ID: 23896429, Hyaluronic acid (HA) has been used in many applications such as pharmaceutical, clinical and cosmetics, so its separation and purification is very important. In this study, firstly D-glucuronic acid imprinted polymers (MIPs) have been synthesized for the separation of HA which has glucuronic acid part in its structure. MIP particles have characterized by elemental analysis, Fourier Transform Infrared Spectroscopy (FT-IR) and swelling tests. Then, synthesized MIP particles have embedded into polyacrylamide based cryogel. Cryogel has prepared by free radical cryogelation process initiated by N,N,N',N'-Tetramethylethylenediamine (TEMED) and ammonium persulfate CAPS) as redox initiators. This cryogel material was characterized by FT-IR, swelling tests, scanning electron microscopy (SEM) and surface adsorption analyze including pore size analyzer (BET) method. The adsorption of HA has investigated by spectrophotometric method using MIPs embedded into cryogel columns (GAIPEC) and the maximum HA adsorption capacity was found to be 318 mgg(-1). The selectivity of GAIPEC column has estimated using N-acetylglucose amine as interfering agent since this molecule is a part of HA and the results have shown that GAIPEC has been nearly 35 times selective for HA than N-acetylglucose amine. The optimum chromatographic conditions for separation of HA were investigated. pH 7.0 buffer solution for elution and 0.1 M of NaCl solution as desorption agent were used at 0.5 mL min(-1) flow rate. Also, recovery of GAIPEC was investigated and the results have shown that GAIPEC could be used many times without decreasing its adsorption capacity significantly. Here in, combining selectivity of MIP particles and mechanical properties of cryogel, a rigid and stable material was prepared for the separation and purification of HA. To point out this, HA has been isolated from fish eye and fermentation of Streptococcus equi RSKK 679 cell culture. After that, it has characterized and Fast Protein Liquid Chromatography (FPLC) applications have been investigated (C) 2013 Elsevier B.V. All rights reserved.

WOS: 000316519900015, In this study, a novel quartz crystal microbalance (QCM) based on the modification of paraoxon imprinted polymer (TCM-Cd(II)-paraoxon) film onto a quartz crystal sensor has been developed for the determination of paraoxon. The sensor is based on a molecular imprinted polymer (MIP) which can be synthesized using paraoxon as a template molecule, Thiourea Modified Chitosan-Cd(II) (TCM-Cd(II)) as the metal-chelate monomer, ephychlorohydrin as a crosslinking agent. The MIP particles have been characterized by FTIR measurements and QCM sensor has characterized using AFM and ellipsometer. The performance of the paraoxon imprinted sensor has indicated that a selective and sensitive paraoxon imprinted sensor could be fabricated. The sensor is able to discriminate paraoxon in solution owing to the specific binding of the imprinted sites. The obtained paraoxon imprinted sensor has 0.02-1 mu M linear range and low detection limit (0.02 mu M). The selectivity studies have shown that the selectivity of prepared paraoxon imprinted sensor has found as being very high in the presence of parathion which is similar in structure with paraoxon. The paraoxon imprinted sensor has been repeatedly used for more than 7 months in many continuous experiments, Eskisehir Osmangazi University, Commission of Scientific Research Projects [200619004], This work has been supported by Eskisehir Osmangazi University, Commission of Scientific Research Projects (Project No: 200619004).

WOS: 000315761700036, PubMed ID: 25427493, As a potential pandemic threat to human health, there has been an urgent need for rapid, sensitive, simpler and less expensive detection method for the highly pathogenic influenza A virus. For this purpose, Quartz Crystal Microbalance (QCM) and Surface Plasmon Resonance (SPR) sensors have been developed for the recognition of hemagglutinin (HA) which is a major protein of influenza A virus. 4-Aminophenyl boronic acid (4-APBA) has been synthesized and used as a new ligand for binding of sialic acid (SA) via boronic acid sugar interaction. SA has an important role in binding of HA. QCM and SPR sensor surfaces have been modified with thiol groups and then 4-APBA and SA have been immobilized on sensor surfaces, respectively. Sensor surfaces have been screened with AFM and used for the determination of HA from aqueous solution. The selective recognition of the QCM and SPR sensors toward Concanavalin A has been reported in this work. Also, the binding capacity and detection limits of QCM and SPR sensors have been calculated and detection limits were found to be 4.7 x 10(-2) mu M, (0.26 mu g ml(-1)) and 1.28 x 10(-1) mu M, (0.72 mu g ml(-1)) in the 95% confidence interval, respectively, Turkey National Boron Research Institute (BOREN) [2008.C0155], Authors thank to Turkey National Boron Research Institute (BOREN) (project number: 2008.C0155) for supporting this study.

WOS: 000303305700055, PubMed ID: 22483924, Molecular imprinted polymers (MIPs) as a recognition element for sensors are increasingly of interest and MIP-nanoparticles have started to appear in the literature. In this study, we have proposed a novel thiol ligand-capping method with polymerizable methacryloylamido-cysteine (MAC) attached to gold-silver-nanoclusters reminiscent of a self-assembled monolayer and have reconstructed surface shell by synthetic host polymers based on molecular imprinting method for cholic acid recognition. In this method, methacryloylamidohistidine-Pt(II)IMAH-Pt(II)] has used as a new metal-chelating monomer via metal coordination-chelation interactions and cholic acid. Nanoshell sensors with templates give a cavity that is selective for cholic acid. The cholic acid can simultaneously chelate to Pt(II) metal ion and fit into the shape-selective cavity. Thus, the interaction between Pt(II) ion and free coordination spheres has an effect on the binding ability of the gold-silver-nanoclusters nanosensor. The binding affinity of the cholic acid imprinted nanoparticles have investigated by using the Langmuir and Scatchard methods and determined affinity constant (K-affinity) has found to be 2.73 x 10(4) mol L-1 and 2.13 x 10(8) mol L-1. respectively. At the last step of this procedure, cholic acid level in blood serum and urine which belong to a healthy people were determined by the prepared gold-silver-nanoclusters

WOS: 000301214700023, Molecular imprinted polymers (MIPs) as a recognition element for sensors are increasingly of interest and MIP-nanoparticles have started to appear in the literature. In this study, we have proposed a novel thiol ligand-capping method with polymerizable methacryloylamido-cysteine (MAC) attached to gold nanoparticles, reminiscent of a self-assembled monolayer and have reconstructed surface shell by synthetic host polymers based on molecular imprinting method for recognition. In this method, methacryloylamidohistidine-Pt(II) [MAH-Pt(II)] has used as a new metal-chelating monomer via metal coordination-chelation interactions and cholic acid. Nanoshell sensors with templates give a cavity that is selective for cholic acid. The cholic acid can simultaneously chelate to Pt(II) metal ion and fit into the shape-selective cavity. Thus, the interaction between Pt(II) ion and free coordination spheres has an effect on the binding ability of the gold nanoparticles nanosensor. The binding affinity of the cholic acid imprinted nanoparticles has investigated by using the Langmuir and Scatchard methods and determined affinity constant (K-affinity) were found as 1.48 x 10(4) mol L-1 and 6.59 x 10(6) mol L-1, respectively. At the last step of this procedure, cholic acid level in blood serum and urine which belong to healthy people was determined by the prepared Au nanosensor

WOS: 000290279000009, PubMed ID: 21437619, This study presents the development of targeted and antibody cross-linked QDs and explores whether these bioconjugates could specifically and effectively label Cu/Zn superoxide dismutase (SOD1) on fixed cells and tissues. QD-antibody conjugation was achieved by using our previously invented AmiNoacid (monomer) Decorated and Light Underpining Conjugation Approach (ANADOLUCA) method. In this method, we have used a photosensitive aminoacid monomer having ruthenium complex which is a synthetic and inexpensive material for the preparation of bioconjugates. Its specificity was demonstrated by extracting the active enzyme from rat liver lysate by using the bioconjugate. It provided accurate antibody orientation, high specificity and mechanic stability. The protocol steps for QD-antibody conjugation and specimen preparation were described in detail. The nanobioconjugates were prepared under mild conditions (for example in day light), independent of pH and temperature, without affecting conformation and function of protein. This protocol is simple, inexpensive and can be successfully adapted to detect other targets on different cell types and tissues.

WOS: 000290577600025, The nanoparticle comprises a superparamagnetic iron oxide nanoparticle core conjugated with trimethoxylsilyl propylmethacrylate (TMSPM) and methacryloylamido serine (MASE), methacryloylamido histidine (MAH), methacryloylamido glutamic acid (MAGA) monomers, and p-nitrophenyl palmitate (p-NPP) which is a substrate of lipase as a template molecule, which enables the creation of lipase active region. The resulting hybrid superparamagnetic nanotraps are magnetically separable, highly active, and stable under harsh conditions. In this study, the advantages of high selectivity of molecular imprinting technique have used to get mimic lipase for the synthesis of methyl jasmonate and methyl oleate.

WOS: 000275134200008, We have used the imprinting technique to create a polymer/organo-smectite nanocomposite catalyst. The aim was preparing a synthetic enzyme which shows high selectivity for paraoxon hydrolysis. Paraoxon imprinted polymer/organo-smectite composites (PIPO) and non-imprinted polymer/organo-smectite composites (NIPO) were prepared using methacryloylhistidine-Cu2+ (MAH-Cu(II)) and [2-methacryloyloxy)ethyl]dimethylhexadecylammonium bromide] (MOEDMAC(16)) modified smectite. These nanocomposites were applied as a catalyst in the hydrolysis of paraoxon which is an organophosphate ester and used as a pesticide. The catalytic activity of these composites was evaluated according to the enzyme kinetics model of Michaelis-Menten. PIPO showed better catalytic activity than NIPO. In addition, the paraoxon imprinted nanocomposite had greater catalytic activity (based on k(cat)/K-m values) for paraoxon hydrolysis by a factor of 391 compared to that of parathion hydrolysis

WOS: 000280240600013, Molecular imprinted polymers (MIPs) as a recognition element for sensors are increasingly of interest and MIP nanoclusters have started to appear in the literature. In this study, we have proposed a novel thiol ligand-capping method with polymerizable methacryloylamido-cysteine (MAC) attached to gold-silver nanoclusters, reminiscent of a self-assembled monolayer and have reconstructed surface shell by synthetic host polymers based on molecular imprinting method for recognition. In this method, methacryloylamidoantipyrine-terbium ((MAAP)(2)-Tb(III)) has been used as a new metal-chelating monomer via metal coordination-chelation interactions and dipicolinic acid (DPA) which is main participant of Bacillus cereus spores used as a model. Nanoshell sensors with templates give a cavity that is selective for DPA. The DPA can simultaneously chelate to Tb(III) metal ion and fit into the shape-selective cavity. Thus, the interaction between Tb(III) ion and free coordination spheres has an effect on the binding ability of the gold-silver nanoclusters nanosensor. The binding affinity of the DPA imprinted nanoclusters has been investigated by using the Langmuir and Scatchard methods, and the respective affinity constants (K (affinity)) determined were found to be 1.43 x 10(4) and 9.1 x 10(6) mol L(-1).

WOS: 000266055400019, PubMed ID: 19362197, Molecular imprinted polymers (MIPs) as a recognition element for sensors are increasingly of interest and MIP nanoclusters have started to appear in the literature. in this study, we have proposed a novel thiol ligand-capping method with polymerizable methacryloylamidocysteine (MAC) attached to gold-silver nanoclusters, reminiscent of a self-assembled monolayer and have reconstructed surface shell by synthetic host polymers based on molecular imprinting method for recognition. In this method, methacryloyl iminodiacetic acid-chrome (MAIDA-Cr(III)) has been used as a new metal-chelating monomer via metal coordination-chelation interactions and dipicolinic acid (DPA) which is a main participant of Bacillus spores has been used as a template. Nanoshell sensors with templates give a cavity that is selective for DPA. The DPA can simultaneously chelate to Cr(III) metal ion and fit into the shape-selective cavity. Thus, the interaction between Cr(III) ion and free coordination spheres has an effect on the binding ability of the gold-silver nanoclusters nanosensor. The binding affinity of the DPA imprinted nanoclusters has been investigated by using the Langmuir and Scatchard methods and determined affinity constants (K-affinity) were found as 18 x 10(6) mol L-1 and 9 x 10(6) mol L-1, respectively

WOS: 000269139900058, The purification of hyaluronic acid (HA) is relatively significant to use in biomedical applications. The structure of HA is formed by the repetitive units of glucuronic acid and N-acetyl glucosamine. In this study, glucuronic acid-imprinted microbeads have been supplied for the purification of HA from cell culture (Streptococcus equi). Histidine-functional monomer. methacryloylamidohistidine (MAH) was chosen as the metal-complexing monomer. The glucuronic acid-imprinted poly(ethyleneglycoldimethacrylate-MAH-Copper(II)) [p(EDMA-MAH-Cu2+)] microbeads have been synthesized by typical suspension polymerization procedure. The template glucuronic acid has been removed by employing 5 M methanolic KOH solution. p (EDMA-MAH-Cu2+) microbeads have been characterized by elemental analysis, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) images and swelling studies. Moreover, HA adsorption experiments have been performed in a batch experimental set-up. Purification of HA from cell culture supernatant has been also investigated by determining the hyaluronidase activity using purified HA as substrate. The glucuronic acid imprinted p(EDMA-MAH-Cu2+) particles can be used many times with no significant loss in adsorption capacities. Also, the selectivity of prepared molecular imprinted polymers (MIP) has been examined. Results have showed that MIP particles are 19 times more selective for glucuronic acid than N-acetylglucose amine

WOS: 000265763500012, PubMed ID: 19362624, The routine measurement of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in biological samples is a difficult analytical problem due to the low levels of the analyte and complex matrix. A new 8-OHdG imprinted quartz crystal microbalance (QCM) sensor has been developed for selective determination of 8-OHdG in serum samples. To fulfil the desired results, we have used methacryloyl aminoantipyrine-Fe(III) [MAAP-Fe(III)] and methacryloyl histidine-Pt(II) [MAH-Pt(II)] as metal-chelating monomers via double metal coordination-chelation interactions for the preparation of additional selective molecular imprinted polymers (MIP). The study includes the measurement of binding interaction of 8-OHdG imprinted quartz crystal microbalance (QCM) sensor, selectivity experiments and analytical performance of QCM chip. The obtained results have showed that the application of double metal-chelate monomer systems has been more effective than single metal-chelate monomer systems. In this study, the detection limit and the linear working range were found to be 0.0075 and 0.0100-3.5 mu M, respectively. The affinity constant (K-affinity) was found to be 1.54 x 10(5) M-1 for 8-OHdG using MAH-Pt-8-OHdG-MAAP-Fe based thin film. Also, selectivity of prepared QCM sensor was found as being very high in the presence of competitive species. At the last step of this procedure, 8-OHdG level in blood serum which belongs to a intestinal cancer patient was determined by the prepared QCM sensor

WOS: 000261262000037, PubMed ID: 18718752, Recently, the 8-hydroxy-2'-deoxyguanosine (8-OHdG) has been used as a marker to determine the oxidative stress. There is no any cheap and easy determination method based on chips and sensor systems for the determination of 8-OHdG. In this study, we have proposed imprinting methods for 8-OHdG recognition and determination using methacryloylamidohistidine-platinum(II) [MAH-Pt(II)] as a new metal-chelating monomer. The study includes the solid-phase extraction (SPE) of blood sample by a new 8-OHdG imprinted sorbent and the measurement of binding interaction of 8-OHdG imprinted quartz crystal microbalance (QCM) sensor via ligand interaction. 8-OHdG imprinted sorbent has prepared by bulk polymerization of MAH-Pt(II) and N-N'-methylenebisacrylamide, 8-OHdG imprinted sensor has prepared on a QCM chip coating the thiol pretreated Au electrode. At the end of these steps, a thin molecular imprinted polymer (MIP) film for the detection of 8-OHdG has developed and analytical performance of QCM sensor which has prepared using MIP was investigated. The affinity constant (K.) for 8-OHdG using MAH-Pt-based thin film has determined by using the Scatchard method. The average percentage recovery of 8-OHdG from plasma samples was found as 80% by using of 8-OHdG imprinted SPE material. At the last step, 8-OHdG level in several blood plasma has been determined by this improved QCM sensor. The obtained results confirmed that the 8-OHdG level in cancer patient's blood was significantly higher than in general subjects, The Scientific and Technical Research Council of Turkey [TOBITAK-MAG-104M374], This study was supported by "The Scientific and Technical Research Council of Turkey" (TOBITAK-MAG-104M374). Furthermore, it has dedicated to the 50th anniversary of Anadolu University.

WOS: 000244369600029, PubMed ID: 19071362, Solid-phase extraction (SPE) based on molecularly imprinted polymers (MIPs) were used to develop selective separation and preconcentration for methylmercury ion from complex matrixes. In this study, an ion-imprinting polymer was prepared to make artificial organomercury lyase preorganizing three methacryloyl-(L)-Cysteine methylester (MAC) monomers and one methylmercury ion in a three-coordinate form by template polymerization, with the goal preparing a solid-phase which has the high selectivity for methylmercury ions. Methylmercury-imprinted beads were produced by a dispersion polymerization technique with use methylmercury-methacryloyl-(L)-cysteine (MM-MAC) complex monomer and ethylene glycoldimethacrylate (EDMA). After removal of methylmercury ions, methylmercury-imprinted beads were used for solid-phase extraction and determination of mercury compounds. Methylmercury adsorption and selectivity studies of methylmercury versus other metal ions which Hg(II), Zn(II), Pb(II) and Cd(II) were reported and distribution and selectivity coefficients of these ions with respect to methylmercury were calculated here. ICP-OES and HPLC-DAD determinations of methylmercury and mercury ions in the certified reference, LUTS-1 from the National Research Council of Canada and synthetic sea water showed that the interfering matrix had been almost removed during preconcentration. The methylmercury-imprinted solid-phase as mimic receptor was good enough for methylmercury determination in complex matrixes

WOS: 000244362500011, PubMed ID: 17074437, The purpose of this study is to prepare a novel molecular imprinted adsorbent to Cr(III) analysis with high selectivity. Initially, chromium (III)-methacryloylhistidine (MAH/Cr(III)) complex monomer was synthesized and then Cr(III) ion imprinted ethyleneglycoldimethacrylate-methacryloylhistidine (poly(EDMA-MAH/Cr(III))) was polymerized. Cr(III) ions were removed from the Cr(III)imprinting polymer. Selective cavity for the chromium (III) ions was obtained in the (poly(EDMA-MAH/Cr(III))) beads. These Cr(III)-imprinted beads were used in the sorption-desorption process. The effect of initial concentration of metal, the sorption rate and the pH of the medium on sorption capacity of Cr(III)-imprinting sorbents were studied. Sorption equilibrium time was achieved in about 30 min. The maximum sorption of Cr(HI) ions onto imprinted beads was about 69.28 mg/g. Sorption studies of CO(H), Ni(II), Cr(III) and Cr(VI) ions were also investigated using Cr(III)-imprinting polymers. The obtained results show that selectively adsorbed amount of Cr(III) ion on Cr(III)-imprinting polymers is higher than all other studied ions. When the beads were used repeatedly, their adsorption capacity was showed feasibility

WOS: 000243674800014, PubMed ID: 16950507, The aim of this study is to prepare ion-imprinted polymers which can be used for the selective removal of mercury ions [Hg2+] from human serum. N-Methacryloyl-(L)-cysteine (MAC) was chosen as the complexing monomer. In the first step, Hg2+ was complexed with MAC and the Hg2+-imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-cysteine) (MIP) beads were synthesized by suspension polymerization. After that. the template ions (i.e., Hg2+) were removed using thiourea (0.5%, v/v) in 0.05 M HCl. The specific surface area of the MIP beads was found to be 59.04 m(2)/g with a size range of 63-140 mu m in diameter and the swelling ratio was 91.5%. According to the elemental analysis results, the MIP beads contained 87.0 mu mol MAC/g polymer. The maximum adsorption capacity was 0.45 mg Hg2+/g beads. The applicability of two kinetic models including pseudo-first order and pseudo-second order model was estimated on the basis of comparative analysis of the corresponding rate parameters, equilibrium capacity and correlation coefficients. Results suggest that chemisorption processes could be the rate-limiting step in the adsorption process. The relative selectivity coefficients of MIP beads for Hg2+/Cd2+, Hg2+/Zn2+ were 14.7 and 21.5 times greater than the non-imprinted (NIP) matrix. respectively. The MIP beads could be used many times without decreasing in their adsorption capacities significantly

Tez (doktora) - Anadolu Üniversitesi, Anadolu Üniversitesi, Fen Bilimleri Enstitüsü, Kimya Anabilim Dalı, Kayıt no: 384405, Multiple Skleroz (MS); özellikle genç erişkinlerde görülen, normal gelişimini tamamlamış olan miyelinin (sinirlerin etrafındaki kılıfın) yıkımı (demiyelinizasyon) ile şekillenen, sebebi tam olarak bilinmeyen santral sinir sisteminde dağınık lezyonların bulunduğu bir hastalıktır. Merkezi sinir sistemi, miyelinin yaklaşık % 35'i farklı izoform ve izomerlerin karışımını içeren miyelin kılıf ile korunmaktadır. Bu miyelin kılıfda en çok bulunan proteinlerden biri olan Miyelin Temel Protein (MBP) dir. MS hastalarında, MBP yapısındaki argininlerin sitruline dönüşmesi söz konusudur. Bu nedenle, sitrulin aminoasidinin tayininin gerçekleştirilmesi ile MS hastalığının varlığı tespit edilebilir. Romatoid Artrit (RA) ise, dünyada ve ülkemizde en sık rastlanılan otoimmün hastalıktır. RA'de sitrulinlenmiş yapılara karşı üretilmiş otoantikorlar uzun süredir bilinmekte ve tanı amacıyla kullanılmaktadır. Anti sitrulinlenmiş siklik protein (anti-CCP) antikorlarının keşfi RA tanısı için önemli bir aşama olmuştur. Bu çalışmada, sitrulin tayini için moleküler baskılama tekniği ile karbon nanotüp (CNT) ve Altın (Au) nanoparçacıkları yüzeyinde sitrulin aminoasidine duyarlı polimerler elde edilmiştir. Bu modifiye polimerler sayesinde MS hastalığında MBP'nin demiyelinizasyonu sonucu ortaya çıkan sitrulin aminoasidinin sitruline özgü bağlanma bölgeleri ile bağlanarak dedeksiyonunun sağlanıp sağlanmadığı araştırılmıştır. Diğer taraftan, RA'de ortaya çıkan ve sitrülinlenmiş yapılara spesifik bir şekilde bağlanabilen Anti-CCP otoantikorlarının sitrülin modifiye polimerle etkileştirildiğinde RA teşhisi gerçekleştirilip gerçekleştirilmediği araştırılmıştır.

WOS: 000349082200113, Tumor necrosis factor (TNF)-alpha promotes rheumatoid arthritis (RA) symptoms, including inflammation and bone erosion. The role of TNF-alpha. in RA is considered to be complex because cytokine is responsible for a wide-range of functions, such as activation of immune cells, stimulation of osteoclast differentiation, and induction of cellular apoptosis. It has appeared that the determination of TNF-alpha concentration in serum might be helpful in the staging and prognosis of diseases. In this study, a simple and sensitive potentiometric sensor method has described for the determination of TNF-alpha in serum. Influences of the membrane composition, concentration and equilibrium time have been investigated on the response properties of the electrode. The sensor has exhibited a visibly enhanced response toward TNF-alpha over the concentration range of 0.1-1.0 mg L-1 with a lower detection limit (LDL) of 0.015 mg L-1 and a slope of 62.2 mV decade(-1). The sensor has demonstrated a quick and reliable response, ability to be reproduced, as well as long-term stability

Tez (yüksek lisans) - Anadolu Üniversitesi, Anadolu Üniversitesi, Fen Bilimleri Enstitüsü, Kimya Anabilim Dalı, Kayıt no: 383411, Bu çalışmada; yaban turbu horseradish peroksidaz (HRP) enziminin iyon değişim kromatografisi ile saflaştırılmasına yönelik 2-akrilamido-2-metil-1-propan sülfonik asit (AMPS) bağlı katyonik değiştirici özellikte kriyojel kolon geliştirilmiş ve bu kolonun HRP saflaştırılmasındaki etkinliği araştırılmıştır. Daha sonra, saflaştırılan HRP kullanılarak kriyojel kolon geliştirilmiş ve bu kolon kullanılarak enzimatik polimerizasyon yöntemi ile iletken poli(3,4-etilendioksitifoyen (PEDOT) polimeri sentezlenmiştir. Bu doğrultuda ilk aşamada; hidroksi etilmetakrilat (HEMA) monomeri, N,N' metilenbisakrilamit (MBA) varlığında polimerleştirilerek AMPS kriyojel kolon hazırlanmıştır. Şişme testi, BET, SEM ve FT-IR analizleri ile hazırlanan kriyojel kolonun karakterizasyonu, Bradford yöntemi ile saflaştırılan HRP' nin protein içeriği ve SDS Page analizi ile molekül ağırlığı belirlenmiştir. Çalışmanın ikinci aşamasında; MBA, HEMA ve rutenyum tabanlı amino asit monomerleriyle fotosensitif çapraz bağlama (ANADOLUCA) yöntemi kullanılarak HRP kriyojel kolon hazırlanmıştır. Daha sonra, HRP kriyojel kolondan poli(sodyum 4-sitirensülfonat) (Na/PSS) ve 3,4-etilendioksitiyofen (EDOT) monomeri ile peristaltik pompa kullanılarak PEDOT/PSS polimeri sentezlenmiştir. Sentezlenen polimerin Jel Geçirgenlik Kromatografisi (GPC) ile molekül ağırlığı belirlenmiştir.

WOS: 000351171600044, In this study, we have aimed to prepare quartz crystal microbalance (QCM) sensor using paraoxon-imprinted particles. Firstly, methacryloyl antipyrine (MAAP)-based metal-chelate-coordinated pre-complex has been prepared and used for paraoxon templation. Then, paraoxon-imprinted nanofilms were formed on QCM sensor after modification of the gold surfaces with allyl mercaptan. By this way, specific and selective memories, which depend on metal-chelate interactions between Eu(III) ions and template, for paraoxon molecules have been obtained on the electrode surface. QCM sensor has characterized using AFM and ellipsometer. The detection limit and the affinity constant have found to be 0.09 mu M and 5.71 x 10(3) M-1 for MAAP-Eu paraoxon-based nanofilm, respectively. The specificity of the QCM sensor has shown using parathion as a competitor molecule., Eskisehir Osmangazi University, Commission of Scientific Research Projects [200619004], This work was been supported by Eskisehir Osmangazi University, Commission of Scientific Research Projects (Project No: 200619004).

WOS: 000237373300003, The aim of this study is to prepare double-imprinted polymers which can be used for the selective removal of Cu(II) ions from synthetic waters. Chitosan-succinate (CS) was choosen as the metal complexing polymer. In the first step, chitosan was modified with succinic anhydrides. Modified chitosan was complexed with copper(II) ions and then reacted with 3-(2-aminoethylamino) propyltrimethoxysilane (AAPTS). In the prepared imprinted sorbent, both Cu(II) and AAPTS were used as templates. Then polymeric beads were crosslinked with tetraethoxysilane (TEOS). After that, the template (i.e., Cu(II) ions) was removed using 0.1 M HNO3 solution. These Cu(II)-imprinted microbeads were used in the adsorption-desorption process. The effect of initial concentration of metal, the adsorption equilibria and the pH of the medium on adsorption capacity of Cu(II)-imprinting sorbents were studied. The maximum adsorption capacity and the relative selectivity coefficients of imprinted beads for Cu(II)/Zn(II), Cu(II)/Ni(II) and Cu(II)/Co(II) were also calculated. The double-imprinted polymers could be used many times without decreasing their adsorption capacities significantly