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A study was conducted to examine that sanguinari, a benzophenanthridine alkaloid strongly induced filamentation in both Gram-positive and Gram-negative bacteria and prevented bacterial cell division by inhibiting cytokinesis. Results of the study suggested that sanguinari inhibited cytokinesis in B. subolis by inhibiting Z-ring formation without reflecting nucleoid segregation.

YfiT, a 19-kDa polypeptide form Bacillus subtilis, belongs to a small sequence family with members predominantly from Gram positibe bacteria. The crystal sturcture of YfiT in complex with Ni(super 2+) to resolution of 1.7 angstrom is demonstrated.

The purification of three membrane-bound c cytochromes from Bacillus azotoformans is described. Their apparent molecular masses on SDS-PAGE are approximately 11 kDa. At neutral pH, these c cytochromes are negatively charged and the Em for all is close to 150 mV.

Pyrene fluorescence spectroscopy and circular dichroism is used to analyze the assembly, structure and stability of nisin pores. The interaction between nisin and its membrane-anchored target lipid II leads to the assembly of pores with a defined number of Lipid II and nisin molecules.

Research has been conducted on the Gram-positive bacteria express cell surface proteins. The interactions between fibronectin-binding protein' ligand binding repeats and fibronectin N-terminal region module pair have been examined.

The discovery of an anionic amphiphilic dendrimer possessing Gram-positive antibacterial activity and minimal eukaryotic cell toxicity is described. This selectivity is of chemical, biological and clinical interest, as antibacterials such as these might be maximally effective against microbial infections without harming the host.

The development of an efficient method to convert thiazolyl peptide antibiotic nocathiacin I isolated from Amicolaptosis species to nocathiacin acid is described. The procedure involved a facile cleavage of dehydroalanine using trifluoroacetic anhydride and pyridine in THF which could also be applied to obtain C-terminal dehydroalanine.

Fosfomycin is a simple phosphonate found to have antibacterial activity against both Gram-positive and Gram-negative microorganisms. The study of the FosA-catalyzed reaction reveals that the early transition state and phosphonoformate acts as a minimal transition state analogue inhibitor.

Combination of Van (vanomycin)-FePt magnetic nanoparticles with a polyvalent ligand- receptor interaction is used for sensitive and quick assay of bacteria at concentration less that 10(super 2) cfu/mL. The protocol that uses such biofunctional magnetic nanoparticles to detect vancomycin-resistant enterococci (VRE) demonstrated capturing of Gram-negative bacteria at a slightly high concentration (15 cfu/mL).