67 results on '"Dong, A-liang"'
Search Results
2. Remote asymmetric conjugate addition catalyzed by a bifunctional spiro-pyrrolidine-derived thiourea catalyst
- Author
-
Fu-Min Zhang, Ai-Jun Ma, Ming-Hui Xu, Jin-Bao Peng, Yong-Hai Yuan, Kun Zhang, Yong-Qiang Tu, Dong-Dong Liang, and Xiao-Ming Zhang
- Subjects
chemistry.chemical_compound ,Thiourea ,chemistry ,Organic Chemistry ,Enantioselective synthesis ,Surface modification ,Stereoselectivity ,Bifunctional ,Combinatorial chemistry ,Pyrrolidine ,Catalysis ,Conjugate - Abstract
A novel spiro-pyrrolidine (SPD)-derived bifunctional thiourea catalyst has been developed, enabling the development of a stereoselective conjugate addition of furfurals to β,γ-unsaturated α-ketoesters. The reaction represents an example of asymmetric trienamine catalysis in the benzylic C–H functionalization of 5-benzylfurfurals, and the rigid spirocyclic framework of the catalyst is proven to be essential for a highly enantioselective transformation.
- Published
- 2021
3. Effect of C/N on partial nitrification in an MBBR at low temperature
- Author
-
Ding Fan, Jun Li, Dong-Bo Liang, Wei Bian, Yaodong Wu, and Dongyue Li
- Subjects
chemistry.chemical_classification ,Environmental Engineering ,biology ,Moving bed biofilm reactor ,0208 environmental biotechnology ,Biofilm ,02 engineering and technology ,010501 environmental sciences ,Polysaccharide ,biology.organism_classification ,01 natural sciences ,020801 environmental engineering ,chemistry.chemical_compound ,Activated sludge ,chemistry ,Nitrifying bacteria ,Nitrification ,Nitrite ,Effluent ,0105 earth and related environmental sciences ,Water Science and Technology ,Nuclear chemistry - Abstract
Two main problems in partial nitrification at low temperature are its difficult start-up and easy inhibition, and this issue significantly limits the broad application of advanced partial nitrification technology. To promote rapid culturing and stabilization of partial nitrification biofilms, two experiments were carried out to investigate the influence of C/N on partial nitrification through ratio control (R, dissolved oxygen (DO)/effluent total ammonia nitrogen (TANeff)) in a lab-scale moving bed biofilm reactor (MBBR) at 12 °C. One experiment looked at the effect of C/N on the stable operation of partial nitrification at 12 °C, which inoculated a mature biofilm with good partial nitrification performance; the other looked at the effect of C/N on the biofilm cultivation and start-up of partial nitrification at 12 °C, which inoculated activated sludge and Pall rings. As for the startup experiments, only the C/N ratio was investigated, as the DO/TANeff ratio was kept constant. The effect of C/N on the mature partial nitrification biofilm showed that the increase of C/N could increase the resistance of partial nitrification to high DO. The nitrite accumulation ratio (NAR, (NO2−-N/(NO2−-N + NO3−-N) × 100%)) increased from 40.41% to 98.78% when R was 0.25 and C/N increased from 0 to 1. Meanwhile, when C/N increased to 2 and the R value slightly increased to 0.3, partial nitrification failed. The penetration of DO in the biofilm indicated that the increase of C/N enhanced the mass transfer resistance of DO, but it was similar when C/N was 1 and 2. Adding an appropriate amount of C/N can reduce the negative effect of partial nitrification damage caused by DO/TAN enlargement. The start-up experiment showed that C/N = 1 and C/N = 2 were essential for culturing biofilms and achieving partial nitrification at 12 °C, which can be successfully achieved (NAR was 85% and 96%) on the 56th and 52nd days. Neither C/N = 0 nor C/N = 3 could effectively achieve the partial nitrification. The increase of C/N will reduce the proportion of nitrifying bacteria in the biofilm, which is harmful to the partial nitrification. This will also lead to the decrease of protein/polysaccharide (PN/PS) and affect the stability of the biofilm.
- Published
- 2020
4. Association Between Hemoglobin Glycation Index and Risk of Cardiovascular Disease and All Cause Mortality in Type 2 Diabetic Patients: A Meta-Analysis
- Author
-
Dong-liang Liang, Hui-qi Hu, Jian-di Wu, Dan Sun, Hai-hong Chen, Yue Xie, and Mei-yu Chen
- Subjects
medicine.medical_specialty ,Disease ,Cardiovascular Medicine ,030204 cardiovascular system & hematology ,hemoglobin glycation index ,Gastroenterology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,cardiovascular disease ,Glycation ,Internal medicine ,Diabetes mellitus ,medicine ,Diseases of the circulatory (Cardiovascular) system ,030212 general & internal medicine ,risk ,business.industry ,Hazard ratio ,medicine.disease ,Confidence interval ,chemistry ,RC666-701 ,Meta-analysis ,all-cause mortality ,Systematic Review ,Hemoglobin ,Glycated hemoglobin ,Cardiology and Cardiovascular Medicine ,business ,glycated hemoglobin - Abstract
Background: The hemoglobin glycation index (HGI) has been proposed as a marker to quantify inter-individual variation in hemoglobin glycosylation. However, whether HGI is associated with an increased risk of diabetic complications independent of glycated hemoglobin (HbA1c) remains unclear. This meta-analysis aimed to determine the association between HGI and the risk of all cause mortality and composite cardiovascular disease (CVD).Methods: PubMed, and EMBASE databases were searched for related studies up to March 31, 2021. Observational studies reported associations between HGI levels and composite CVD and all cause mortality were included for meta-analysis. A random effect model was used to calculate the hazard ratios (HRs) and 95% confidence intervals (CI) for higher HGI.Results: A total of five studies, comprising 22,035 patients with type two diabetes mellitus were included for analysis. The median follow-up duration was 5.0 years. After adjusted for multiple conventional cardiovascular risk factors, an increased level of HGI was associated with a higher risk of composite CVD (per 1 SD increment: HR = 1.14, 95% CI = 1.04–1.26) and all cause mortality (per 1 SD increment: HR = 1.18, 95% CI = 1.05–1.32). However, when further adjusted for HbA1c, the association between HGI and risk of composite CVD (per 1 SD increment of HGI: HR = 1.01, 95% CI = 0.93–1.10) and all cause mortality (per 1 SD increment of HGI: HR = 1.03, 95% CI = 0.96–1.10) became insignificant.Conclusions: High HGI was associated with an increased risk of composite CVD and all cause mortality after adjustment for multiple conventional cardiovascular risk factors. However, the association was mainly mediating by the level of HbA1c.
- Published
- 2021
5. The influence of the novel composite material LiNbO3@Fe3O4 on the denitrification efficiency of bacterium Achromobacter sp. A14
- Author
-
Jia Xing Wang, Jun Feng Su, Yuan ming Zhang, Dong hui Liang, Lei He, and Xue chen Bai
- Subjects
Denitrification ,biology ,Strain (chemistry) ,0208 environmental biotechnology ,02 engineering and technology ,General Medicine ,010501 environmental sciences ,biology.organism_classification ,01 natural sciences ,020801 environmental engineering ,Achromobacter sp ,chemistry.chemical_compound ,Nitrate ,chemistry ,Environmental Chemistry ,Autotroph ,Composite material ,Waste Management and Disposal ,Bacteria ,0105 earth and related environmental sciences ,Water Science and Technology - Abstract
The effect of the novel composite material LiNbO3@Fe3O4 on the nitrate removal, and Mn2+ oxidation efficiency by autotrophic denitrification strain Achromobacter sp. A14 was investigated in this st...
- Published
- 2019
6. Multifunctional sugar-cube-like Fe3O4@Cu/PVA biomaterials for enhanced removal of nitrate and Mn(II) from moving bed biofilm reactor (MBBR)
- Author
-
Dong hui Liang, Li Wei, Hai rong Zhu, Xue Chen Bai, Yi chou Gao, and Jun feng Su
- Subjects
0106 biological sciences ,chemistry.chemical_compound ,Nitrate ,Chemical engineering ,Chemistry ,Moving bed biofilm reactor ,010608 biotechnology ,010501 environmental sciences ,Cube ,Sugar ,01 natural sciences ,0105 earth and related environmental sciences ,Water Science and Technology - Abstract
A novel Fe3O4@Cu/PVA biomaterial as a new adsorbent and bacterial cell immobilized carrier was synthesized in this work. The structure and morphology were characterized by scanning electron microscopy (SEM). Effects of factors on Mn(II)-based autotrophic denitrification were investigated in a moving bed biofilm reactor (MBBR). The results indicate that the highest nitrate removal and Mn(II) oxidation efficiency occurred under the conditions of initial Mn(II) concentration of 80 mg·L−1, hydraulic retention time (HRT) of 10 h and pH 7. Meteorological chromatography analysis showed that N2 was produced as an end-product, and that gas compositions were different depending on the concentration of Mn(II) in the MBBR. The community diversity in the MBBR was markedly influenced by the concentration of Mn(II) and Pseudomonas sp. H-117 played a primary role in the process of nitrate removal and Mn(II) oxidation.
- Published
- 2019
7. Fabrication of h-BN@PbWO4 with a facile sol-gel method towards enhanced photocatalytic and radiation shielding properties
- Author
-
Dui-Gong Xu, Ming Wei, Xue-Yuan Bai, You Wu, Jun-Hua Liu, Yuan-Lin Zhou, Dong-Ming Liang, Quan-Ping Zhang, and Wen-Fan Zhu
- Subjects
Materials science ,Fabrication ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Inorganic Chemistry ,chemistry.chemical_compound ,Materials Chemistry ,Rhodamine B ,Neutron ,Physical and Theoretical Chemistry ,Fourier transform infrared spectroscopy ,Sol-gel ,business.industry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,0104 chemical sciences ,Electronic, Optical and Magnetic Materials ,Radiation shielding ,chemistry ,Chemical engineering ,Ceramics and Composites ,Photocatalysis ,Radiation protection ,0210 nano-technology ,business - Abstract
Designing versatile materials for radiation protection is of great significance to the rapid development of nuclear technology. Here, BN@PbWO4 core-shell particles are successfully prepared via a facile sol-gel method. The structure and morphology of products are determined by XRD, SEM, TEM and FTIR techniques. Compared with PbWO4 and h-BN, BN@PbWO4 exhibits much more excellent photocatalytic ability to degrade the organic dye Rhodamine B (RhB). Furthermore, it also has a good ability to jointly shield neutron and gamma radiations, especially better gamma radiation shielding in comparison with PbWO4. This work provides an integrated solution with the material that could deal with organic wastes and shield complex radiations in nuclear industry.
- Published
- 2019
8. Up-regulation of DcR3 in microbial toxins-stimulated HUVECs involves NF-κB signalling
- Author
-
Hongwei Chen, Dong-Yu Liang, Yang Liu, Xiaoli Lou, and Yanqiang Hou
- Subjects
Lipopolysaccharides ,0301 basic medicine ,Proline ,Lipopolysaccharide ,Pyridines ,lcsh:Animal biochemistry ,Pharmacology ,Biochemistry ,Jurkat cells ,Umbilical vein ,NF-κB ,Sepsis ,lcsh:Biochemistry ,Jurkat Cells ,03 medical and health sciences ,chemistry.chemical_compound ,Thiocarbamates ,Human Umbilical Vein Endothelial Cells ,medicine ,Humans ,lcsh:QD415-436 ,Protein Kinase Inhibitors ,Molecular Biology ,lcsh:QP501-801 ,HUVECs ,Anthracenes ,Receptors, Tumor Necrosis Factor, Member 6b ,Zymosan ,Imidazoles ,NF-kappa B ,medicine.disease ,Up-Regulation ,Teichoic Acids ,030104 developmental biology ,chemistry ,Lipoteichoic acid ,Decoy receptor 3 ,DcR3 ,Research Article ,Signal Transduction - Abstract
Background Sepsis is a severe condition characterised by the body’s systemic inflammatory response to infection. The specific sepsis-related biomarkers should be used in clinical diagnosis, therapeutic response monitoring, rational use of antibiotics, and prognosis (risk stratification), etc. Results In this study, we investigated the expression level of Decoy Receptor 3 (DcR3) and the mechanism of high expression in sepsis patients. Septic cell model experiments were performed by treating human umbilical vein endothelial cells (HUVECs) and Jurkat cells with lipopolysaccharide (LPS), lipoteichoic acid (LTA) and zymosan, respectively. SP600125, SB203580 and ammonium pyrrolidinedithiocarbamate (PDTC) were used to inhibit JNK1/2, p38MAPK and NF-κB signalling pathways in septic cell model, respectively. These results showed that DcR3 levels were higher in sepsis group than control. DcR3 mRNA and protein levels in HUVECs were increased following treatment with LPS, LTA and zymosan, and also increased in Jurkat cells treated by LPS, but not by LTA or zymosan. When HUVECs were treated with the NF-κB inhibitor PDTC, DcR3 expression was decreased compared with controls. However, SP600125 and SB203580 had no effect on DcR3 mRNA or protein levels. Conclusions The results indicated that DcR3 secretion proceeded through the NF-κB signalling pathway in HUVECs.
- Published
- 2018
9. SuFExable polymers with helical structures derived from thionyl tetrafluoride
- Author
-
Dong-Dong Liang, Gencheng Li, Peng Wu, Feng Zhou, K. Barry Sharpless, Jianmei Lu, Bing Gao, Hafedh Driss, Xiaoyan Chen, Yi Liu, John E. Moses, Sidharam P. Pujari, Hyunseok Kim, Liana M. Klivansky, Han Zuilhof, and Suhua Li
- Subjects
chemistry.chemical_classification ,Thionyl tetrafluoride ,General Chemical Engineering ,Aryl ,Organic Chemistry ,General Chemistry ,Polymer ,Combinatorial chemistry ,Organische Chemie ,chemistry.chemical_compound ,chemistry ,Polymerization ,Covalent bond ,Chemical Sciences ,Click chemistry ,Copolymer ,Life Science ,Functional polymers ,VLAG - Abstract
Sulfur(vi) fluoride exchange (SuFEx) is a category of click chemistry that enables covalent linking of modular units through sulfur(vi) connective hubs. The efficiency of SuFEx and the stability of the resulting bonds have led to polymer chemistry applications. Now, we report the SuFEx click chemistry synthesis of several structurally diverse SOF4-derived copolymers based on the polymerization of bis(iminosulfur oxydifluorides) and bis(aryl silyl ethers). This polymer class presents two key characteristics. First, the [–N=S(=O)F–O–] polymer backbone linkages are themselves SuFExable and undergo precise SuFEx-based post-modification with phenols or amines to yield branched functional polymers. Second, studies of individual polymer chains of several of these new materials indicate helical polymer structures. The robust nature of SuFEx click chemistry offers the potential for post-polymerization modification, enabling the synthesis of materials with control over composition and conformation. Sulfur(vi) fluoride exchange (SuFEx)—a type of click chemistry that generates SVI-centred covalent linkages—has previously been used for polymer synthesis. Now, modular SuFEx polymerization using SOF4 has been used to generate helical polymers. Unlike previous examples of SuFEx polymerization, the backbone retains SVI–F motifs and therefore is able to undergo further SuFEx click reactions, enabling facile and efficient post-polymerization modification.
- Published
- 2021
10. Effect of preservation time of formalin-fixed paraffin-embedded tissues on extractable DNA and RNA quantity
- Author
-
Nai-Yan Zeng, Shuang Sha, Rong Yang, Qingqing Yi, Qing Chang, Junfeng Shi, and Dong-Yu Liang
- Subjects
0301 basic medicine ,China ,Medicine (General) ,Time Factors ,Tissue Fixation ,storage time ,Formalin fixed paraffin embedded ,preservation ,DNA and RNA quantity ,Real-Time Polymerase Chain Reaction ,Biochemistry ,Specimen Handling ,FFPE tissues ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,R5-920 ,Formaldehyde ,Humans ,nucleic acid extraction ,Medicine ,Paraffin Embedding ,business.industry ,Gene Expression Profiling ,Biochemistry (medical) ,RNA ,DNA ,Cell Biology ,General Medicine ,Molecular biology ,030104 developmental biology ,chemistry ,030220 oncology & carcinogenesis ,internal reference gene amplification ,business ,Retrospective Clinical Research Report - Abstract
Objectives This study aimed to investigate the factors affecting the quantity of DNA and RNA extractable from human formalin-fixed paraffin-embedded (FFPE) tissues stored for different lengths of time. Methods We randomly selected 20 FFPE specimens harvested from hysteromyoma patients with uterine fibroids during 2010, 2015, and 2017 at the Department of Pathology, Jiading District Central Hospital Affiliated Shanghai University of Medicine and Health Sciences. DNA and RNA extractions were performed using a DNA/RNA FFPE kit. DNA and RNA concentrations and their OD260/OD280 ratios were determined by a NanoDrop 2000 spectrophotometer. The human β-globin gene and aldehyde dehydrogenase-2 (ALDH2) gene were amplified from nucleic acids using a LightCycler 480 Real-Time PCR System, and PCR amplification products were electrophoresed on 1% agarose gels. Results Specimens that were stored for longer showed more degradation and a reduced concentration of DNA and RNA after nucleic acid extraction. However, there was no significant difference in DNA or RNA purity. β-globin and ALDH2 genes could be amplified from more than 99% of specimens. Conclusion We found that FFPE tissues stored for longer had a reduced quantity of extractable DNA and RNA. However, these tissues could be used for the analysis of some small target genes.
- Published
- 2020
11. Preparation and antitumor effects of 4-amino-1,2,4-triazole Schiff base derivative
- Author
-
Guo-Wei Jiang, Dong-Yu Liang, Yong-Jun Meng, Qing Chang, Yu-Ting Zhang, and Qingqing Yi
- Subjects
4-amino-1,2,4-triazole Schiff base derivative ,Medicine (General) ,Cell Survival ,reduction ammonification reaction ,Antineoplastic Agents ,030204 cardiovascular system & hematology ,anticancer ,Biochemistry ,Pre-Clinical Research Report ,Nucleophilic substitution ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,R5-920 ,Cell Line, Tumor ,Humans ,Medicine ,MTT assay ,Schiff Bases ,Cell Proliferation ,Schiff base ,Dose-Response Relationship, Drug ,Triazines ,business.industry ,Biochemistry (medical) ,1,2,4-Triazole ,product characterization ,Cell Biology ,General Medicine ,Triazoles ,Combinatorial chemistry ,chemistry ,A549 Cells ,Benzaldehydes ,030220 oncology & carcinogenesis ,Hepatocytes ,business ,Derivative (chemistry) - Abstract
Objectives The purpose of this paper was to synthesize 4-amino-1,2,4-triazole Schiff base derivative and to evaluate its antitumor activity. Methods 2,4,6-Tris (4-formylphenoxy)-1,3,5-triazine was synthesized by nucleophilic substitution with reaction of p -hydroxybenzaldehyde with cyanuric chloride. Then, the intermediate was reacted with 1,2,4-triazole to form 4-amino-1,2,4-triazole Schiff base derivative by reduction and ammonification of aldehydes. The 4-amino-1,2,4-triazole Schiff base derivative was characterized by infrared spectroscopy, nuclear magnetic resonance, and elemental analyses. Finally, the anticancer effect of 4-amino-1,2,4-triazole Schiff base derivative was evaluated by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay on a lung adenocarcinoma cell line (A549) and a human hepatoma cell line (Bel7402). Results The product synthesized in this study was confirmed to be 4-amino-1,2,4-triazole Schiff base derivative, and it significantly suppressed the proliferation of cancer cell lines in a dose-dependent manner. Conclusions We successfully prepared 4-amino-1,2,4-triazole Schiff base derivative and verified its antitumor activity.
- Published
- 2020
12. Silicon-Free SuFEx Reactions of Sulfonimidoyl Fluorides : Scope, Enantioselectivity, and Mechanism
- Author
-
Jorden Wagemakers, Daan Jordaan, Han Zuilhof, Jacob Baggerman, Dong Dong Liang, and Dieuwertje E. Streefkerk
- Subjects
Reaction mechanism ,Silicon ,Kinetics ,chemistry.chemical_element ,010402 general chemistry ,01 natural sciences ,Catalysis ,chemistry.chemical_compound ,enantioselectivity ,Phenol ,Moiety ,SuFEx ,Research Articles ,VLAG ,010405 organic chemistry ,Organic Chemistry ,Enantioselective synthesis ,General Medicine ,General Chemistry ,Combinatorial chemistry ,Organische Chemie ,0104 chemical sciences ,reaction mechanisms ,chemistry ,Mechanism (philosophy) ,kinetics ,Yield (chemistry) ,Click Chemistry ,Research Article - Abstract
SuFEx reactions, in which an S−F moiety reacts with a silyl‐protected phenol, have been developed as powerful click reactions. In the current paper we open up the potential of SuFEx reactions as enantioselective reactions, analyze the role of Si and outline the mechanism of this reaction. As a result, fast, high‐yielding, “Si‐free” and enantiospecific SuFEx reactions of sulfonimidoyl fluorides have been developed, and their mechanism shown, by both experimental and theoretical methods, to yield chiral products., The SuFEx reaction is shown to proceed quantitatively and fast with wide scope without silyl protection of the phenol. Mechanistic studies reveal a stereospecific bimolecular nucleophilic substitution.
- Published
- 2020
13. Effect of organic matters on anammox coupled denitrification system: when nitrite was sufficient
- Author
-
Xiaoran Ma, Liangang Hou, Jingyue Yang, Zhaoming Zheng, Dong-Bo Liang, Yi-Qi Sun, and Jun Li
- Subjects
0106 biological sciences ,Denitrification ,cod/no2−-n ratios ,010501 environmental sciences ,01 natural sciences ,Biochemistry and Biophysics ,Denitrifying bacteria ,chemistry.chemical_compound ,Ammonia ,010608 biotechnology ,Organic matter ,Nitrite ,nitrite ,lcsh:Science ,0105 earth and related environmental sciences ,chemistry.chemical_classification ,nitrogen removal performance ,Multidisciplinary ,Chemical oxygen demand ,chemistry ,Anammox ,Environmental chemistry ,carbon source ,anammox ,lcsh:Q ,Sodium acetate ,Research Article - Abstract
Anaerobic ammonia oxidation (anammox) and denitrification can work together to weaken the influence of organic matter on anaerobic ammonia oxidation bacteria (AAOB) and improve nitrogen removal performance. As the common substrate of anammox and denitrification, nitrite will also affect nitrogen removal performance when it is insufficient, which is not conducive to reflect the endurance of anammox reactor to organic matter. The UASB continuous flow experiment was carried out to investigate the effect of the concentration of glucose and sodium acetate on nitrogen removal performance of anammox reactor under the condition of sufficient nitrite. With glucose as the organic matter, when the chemical oxygen demand (COD) concentration increased to 200 mg l −1 , nitrogen removal performance of the system began to deteriorate significantly, and the anammox activity was significantly inhibited. With sodium acetate as the organic substance, the anammox activity was affected when the COD was 20 mg l −1 . Adequate nitrite could relieve the inhibition of the coupling system by a low concentration (COD < 200 mg l −1 ) of glucose organic matter. However, it could not relieve the inhibitory effect of sodium acetate. With the increase of organic concentration, the biological density of AAOB in granular sludge gradually decreased, while the biological density of denitrifying bacteria increased gradually.
- Published
- 2019
14. Liquid-like surface modification for effective anti-scaling membrane distillation with uncompromised flux
- Author
-
Yuanyuan Zhang, Cheng-Hui Luo, Fangang Meng, Dong-Qing Liang, and Yu-Xi Huang
- Subjects
Materials science ,Silanes ,Nanoparticle ,Filtration and Separation ,Membrane distillation ,Biochemistry ,Silane ,Polyvinylidene fluoride ,Surface coating ,chemistry.chemical_compound ,Membrane ,Chemical engineering ,chemistry ,Surface modification ,General Materials Science ,Physical and Theoretical Chemistry - Abstract
Membrane scaling is a critical challenge for membrane distillation (MD), especially when treating hypersaline brines. In this study, an anti-scaling membrane was developed with a liquid-like surface coating. This fabrication approach combined electrospun polyvinylidene fluoride (PVDF) membrane with surface fluorination without the addition of nanoparticles. Two fluoro silanes with the same alkyl-chain length but different hydrolysis reaction sites were investigated as the fluorination agents. Membrane characterizations indicated that surface modification with the single hydrolysis site fluoro silane (17-FAS-S) did not change the membrane morphology or structure. On the other hand, surface modification with the triple hydrolysis site fluoro silane (17-FAS-T) generated nanoparticles on the membrane surface, resulting in increased roughness. While both the 17-FAS-S and 17-FAS-T modified membranes exhibited excellent scaling resistance in the MD scaling tests, the 17-FAS-S membrane provided a much higher vapor flux than the 17-FAS-T one. Finally, the mechanism of scaling resistance and vapor permeability provided by the liquid-like modification was discussed.
- Published
- 2021
15. Simultaneous removal of Fe3+ and nitrate in the autotrophic denitrification immobilized systems
- Author
-
Dong hui Liang, Ting lin Huang, Jun feng Su, Wen dong Wang, and Ting ting Lian
- Subjects
0301 basic medicine ,Denitrification ,Chromatography ,Hydraulic retention time ,Strain (chemistry) ,biology ,Pellets ,Enterobacter ,010501 environmental sciences ,biology.organism_classification ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,Nitrate ,chemistry ,Response surface methodology ,Autotroph ,0105 earth and related environmental sciences ,Water Science and Technology - Abstract
In this study, strain CC76, identified as Enterobacter sp., was tested for the reduction of Fe3+ and denitrification using immobilized pellets with strain CC76 as experimental group (IP) and immobilized pellets with strain CC76 and magnetite powder as experimental group (IPM) in the autotrophic denitrification immobilized systems (ADIS). Compared with IP, a higher nitrate removal rate was obtained with IPM by using three levels of influent Fe3+ (0, 5, and 10 mg/L), four levels of pH (5.0, 6.0, 7.0, and 8.0), and three levels of hydraulic retention time (HRT) (12, 14, and 16 h), respectively. Furthermore, response surface methodology (RSM) analysis demonstrated that the optimum removal ratios of nitrate of 87.21% (IP) and 96.27% (IPM) were observed under the following conditions: HRT of 12 h, pH of 7.0 and influent Fe3+ concentration of 5 mg/L (IP) and 1 mg/L (IPM).
- Published
- 2017
16. Microcystis aeruginosa flour as carbon and nitrogen source for aerobic denitrification and algicidal effect of Raoultella sp. R11
- Author
-
Ting lin Huang, Jin suo Lu, Jun feng Su, Min Ma, Dong hui Liang, and Ting ting Lian
- Subjects
0106 biological sciences ,Environmental Engineering ,Denitrification ,biology ,chemistry.chemical_element ,010501 environmental sciences ,Management, Monitoring, Policy and Law ,biology.organism_classification ,01 natural sciences ,Algal bloom ,chemistry.chemical_compound ,Nitrate ,chemistry ,010608 biotechnology ,Aerobic denitrification ,Environmental chemistry ,Botany ,Microcystis aeruginosa ,Sewage treatment ,Response surface methodology ,Carbon ,0105 earth and related environmental sciences ,Nature and Landscape Conservation - Abstract
Harmful algal blooms (HABs) could be deemed hazardous materials in aquatic environments. In this study, we investigated aerobic denitrification and algicidal effects of the Raoultella sp. R11. Based on PCR amplification, the denitrification genes nap A was detected. A response surface methodology (RSM) analysis demonstrated that the maximum nitrate removal ratio was 96.06%, and optimal conditions occurred for inoculum of 13.02% (v/v), initial pH of 6.65, C/N ratio of 7.59 and temperature of 27.08 °C based on a ridge analysis. When the Microcystis aeruginosa flour as carbon and nitrogen source for denitrification in aerobic conditions, the NO 3 − -N and TOC removal ratios were 72.36% and 85.56%, respectively. Additionly, the denitrification rate was 0.0123 mg L −1 h −1 . Furthermore, the infrared spectrogram and SEM images showed the M. aeruginosa cell structure was destroyed, indicating that R11 could use algal death for denitrification. Therefore, Raoultella sp. R11 played a crucial role for simultaneous denitrification and algicidal effects in wastewater treatment.
- Published
- 2017
17. Enhancement of simultaneous algicidal and denitrification of immobilized Acinetobacter sp. J25 with magnetic Fe3O4 nanoparticles
- Author
-
Jun feng Su, Jinsuo Lu, Li Wei, Min Ma, Ting lin Huang, and Dong hui Liang
- Subjects
0301 basic medicine ,Denitrification ,Health, Toxicology and Mutagenesis ,010501 environmental sciences ,01 natural sciences ,Microbiology ,03 medical and health sciences ,chemistry.chemical_compound ,Nitrate ,Microcystis ,Environmental Chemistry ,Microcystis aeruginosa ,0105 earth and related environmental sciences ,biology ,General Medicine ,Acinetobacter ,equipment and supplies ,biology.organism_classification ,Pollution ,030104 developmental biology ,Microbial population biology ,chemistry ,Eutrophication ,human activities ,Bacteria ,Nuclear chemistry - Abstract
In this study, immobilization technique was employed to improve simultaneous algicidal and denitrification of immobilized Acinetobacter sp. J25 with magnetic Fe3O4 in eutrophic landscape water. After 7 days of operation, the maximum superoxide dismutase (SOD) activity (54.43 U mg-1), nitrate removal efficiency (100% (0.2127 mg L-1 h-1)), and chlorophyll-a removal efficiency (89.71%) were obtained from the immobilized J25 with magnetic Fe3O4. The results suggest that immobilized J25 with magnetic Fe3O4 had better nitrogen removal efficiency and algicidal activity in eutrophic landscape water. High-throughput sequencing data profiled the strain J25 that was immobilized with magnetic Fe3O4 which changed the composition of the microbial community. The results indicated a novel concept of enhancing the algicidal and denitrification property of immobilized bacteria with magnetic Fe3O4 in eutrophic landscape water.
- Published
- 2017
18. Mixed electron donor autotrophic denitrification processes for groundwater treatment by immobilized biological filters
- Author
-
Ting ting Lian, Jun feng Su, Dong hui Liang, Wen dong Wang, and Ting lin Huang
- Subjects
chemistry.chemical_classification ,Denitrification ,Hydraulic retention time ,Strain (chemistry) ,0208 environmental biotechnology ,Inorganic chemistry ,Electron donor ,02 engineering and technology ,010501 environmental sciences ,Electron acceptor ,01 natural sciences ,020801 environmental engineering ,chemistry.chemical_compound ,Nitrate ,chemistry ,Autotroph ,Groundwater ,0105 earth and related environmental sciences ,Water Science and Technology - Abstract
An immobilized biological filter (IBF) using Fe(II) and Mn(II) as mixed electron donors was evaluated for nitrate removal in groundwater. Results of the single factor experiments of strain SZ28 under the conditions of electron donor:electron acceptor ratio (1:2, 1.45:1, 3:1), Fe(II):Mn(II) ratio (1:9, 3:7, 5:5) demonstrated that the highest nitrate removal ratio was 100%, 49.6% (Mn(II)) and 100% (Fe(II)) under the conditions of electron donor:electron acceptor ratio of 3:1, Fe(II):Mn(II) ratio of 5:5. Mn(II) and Fe(II) as electron donor was tested for the effects on denitrification in the IBF reactor. Optimal conditions were obtained at an electron donor:electron acceptor ratio of 2:1, hydraulic retention time of 12 h and Fe(II):Mn(II) ratio of 5:5 with the highest removal ratio of nitrate-N (100%), Mn(II) (50.25%) and Fe(II) (99.2%). Results suggest that the optimal condition obtained from the IBF was feasible.
- Published
- 2017
19. Synthesis and conformational structure of hydrazo-bridged homo calix[2]pyridine[2]triazines
- Author
-
Dong-Dong Liang and Mei-Xiang Wang
- Subjects
Denticity ,010405 organic chemistry ,Stereochemistry ,Organic Chemistry ,Hydrazine ,010402 general chemistry ,Ring (chemistry) ,01 natural sciences ,Medicinal chemistry ,0104 chemical sciences ,chemistry.chemical_compound ,Molecular recognition ,chemistry ,Nucleophilic aromatic substitution ,Pyridine ,Moiety ,Triazine - Abstract
Presented in this paper are the practical synthesis and conformational structure of hydrazo-bridged homo calix[4]arenes. The step-wise fragment coupling method based on the nucleophilic aromatic substitution reaction of 2,6-dihydrazinylpyridine with 6-alkoxy-2,4-dichlorotriazine afforded effectively hydrazo-linked homo calix[2]pyridine[3]triazines. The exhaustive methylation of –NHNH– linkages led to lipophilic macrocycles, while the introduction of a THP-protected triglyme moiety on a triazine ring produced a water soluble hydrazo-bridged homo calix[2]pyridine[2]triazine. The acquired hydrazo-bridged homo calix[4]arenes adopted a cone conformational structure with two nitrogen atoms of the hydrazine linkage forming respectively a conjugation system with pyridine and triazine rings. The unique macrocyclic conformation and multidentate binding sites would render hydrazo-bridged homo calix[2]pyridine[2]triazines powerful hosts in molecular recognition and self-assembly.
- Published
- 2017
20. Correlation between liver cancer pain and the HIF-1 and VEGF expression levels
- Author
-
Gui-Yin Feng, Yuan Yuan, Geng Zhang, Yan-Ru Guo, Dong-Qi Liang, and Hai-Lun Wang
- Subjects
0301 basic medicine ,Oncology ,Cancer Research ,medicine.medical_specialty ,Visual analogue scale ,Biology ,medicine.disease_cause ,liver cancer ,visual analogue scale ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Internal medicine ,medicine ,pain ,hypoxia-inducible factor ,Hepatitis ,vascular endothelial growth factor ,Oncogene ,Cancer ,Articles ,medicine.disease ,Molecular medicine ,Vascular endothelial growth factor ,030104 developmental biology ,chemistry ,correlation ,030220 oncology & carcinogenesis ,Carcinogenesis ,Liver cancer - Abstract
A possible correlation between liver cancer pain and the hypoxia-inducible factor (HIF)-1 and vascular endothelial growth factor (VEGF) expression levels was examined. From January, 2015 to January, 2016, 30 patients suffering from liver cancer with pain, 30 patients with liver cancer without pain and 30 hepatitis patients with pain were enrolled in the study. Pain level was evaluated by visual analogue scale (VAS), the expression levels of HIF-1 and VEGF mRNA were determined by RT-PCR and the expression levels of HIF-1 and VEGF proteins were examined by ELISA. Before intervention, the VAS in the hepatitis group was significantly higher than that of the liver cancer pain group. However, after intervention the VAS in the two groups was reduced. HIF-1 and VEGF mRNA expression levels in the liver cancer pain group were significantly higher than those in the liver cancer group before and after intervention. The expression levels of HIF-1 and VEGF mRNA in the hepatitis group were the lowest. The expression levels of HIF-1 and VEGF mRNA in the liver cancer pain group considerably increased after intervention. The expression levels of HIF-1 and VEGF mRNA in the other two groups showed no changes before or after intervention. Before and after the intervention, VAS in the liver cancer pain group was positively correlated to the expression levels of HIF-1 and VEGF. Thus, pain occurrence and the pain level in liver cancer patients were correlated with the expression levels of HIF-1 and VEGF. As the regular three-step medicine analgesic ladder is ineffective in these cases, verification of HIF-1 and VEGF expression levels may be considered the new target for pain release.
- Published
- 2016
21. Simultaneous sulfamethoxazole biodegradation and nitrogen conversion by Achromobacter sp. JL9 using with different carbon and nitrogen sources
- Author
-
Yongyou Hu and Dong hui Liang
- Subjects
0106 biological sciences ,Nitrogen balance ,Environmental Engineering ,Sulfamethoxazole ,Nitrogen ,chemistry.chemical_element ,Bioengineering ,Achromobacter ,010501 environmental sciences ,Bacterial growth ,urologic and male genital diseases ,01 natural sciences ,chemistry.chemical_compound ,010608 biotechnology ,medicine ,Waste Management and Disposal ,0105 earth and related environmental sciences ,Renewable Energy, Sustainability and the Environment ,General Medicine ,Biodegradation ,bacterial infections and mycoses ,female genital diseases and pregnancy complications ,Carbon ,Achromobacter sp ,Biodegradation, Environmental ,chemistry ,Environmental chemistry ,Sodium acetate ,medicine.drug - Abstract
This study investigated sulfamethoxazole (SMX) biodegradation and nitrogen conversion by Achromobacter sp. JL9 using different carbon and nitrogen sources. Results showed that SMX and sodium acetate could be co-metabolized as carbon sources for bacterial growth and nitrogen conversion with highest removal efficiencies of 82.44%, 80.2%, and 79.45% for NH4+-N, NO3--N, and SMX, respectively. Strain JL9 was able to utilize SMX as its sole nitrogen source for growth, with an SMX biodegradation efficiency of 63.10%. In addition, carbon and nitrogen balance analyses showed that approximately 35.31% and 63.22% of carbon and nitrogen, respectively, were lost as gaseous products. Finally, medium toxicity gradually decreased during the carbon and nitrogen dependence experiments. This study, thus, suggests that carbon and nitrogen play vital roles in SMX biodegradation and biotoxicity reduction.
- Published
- 2019
22. Performance and microbial community of an immobilized biofilm reactor (IBR) for Mn(II)-based autotrophic and mixotrophic denitrification
- Author
-
Yuan ming Zhang, Zhao Wang, Dong hui Liang, Jia Xing Wang, Jun Feng Su, and Min Li
- Subjects
0106 biological sciences ,Environmental Engineering ,Denitrification ,Hydraulic retention time ,Bioengineering ,010501 environmental sciences ,complex mixtures ,01 natural sciences ,chemistry.chemical_compound ,Bioreactors ,Nitrate ,010608 biotechnology ,Autotroph ,Waste Management and Disposal ,0105 earth and related environmental sciences ,Autotrophic Processes ,Nitrates ,biology ,Renewable Energy, Sustainability and the Environment ,Microbiota ,Biofilm ,General Medicine ,biology.organism_classification ,chemistry ,Microbial population biology ,Environmental chemistry ,Biofilms ,Proteobacteria ,Mixotroph - Abstract
An immobilized biofilm reactor (IBR) was established to treat nitrate using different electron donors. A novel material, Fe3O4@Cu/PVA, was synthesized as an adsorbent and bacterial immobilized carrier in the reactor. The optimum condition of nitrate removal were pH 7.0, hydraulic retention time (HRT) of 10 h under autotrophic and mixotrophic conditions. Strain H-117 in the mixotrophic reactor had better adaptability to changes in the initial pH. The metabolism in the mixotrophic reactor was more vigorous than that in autotrophic reactor. The microbial communities and structures were evaluated to determine the nitrate removal mechanisms in this system. Microbial analyses demonstrated that different electron donor could influence the bacterial abundance and species in the IBR system. Proteobacteria was the most dominant phylum in all IBRs and accounted for more than 50% of the total phyla. Pseudomonas and Rhizobium were the dominant contributor to the effective removal of nitrate in the IBRs.
- Published
- 2019
23. Polyfunctionalized biaryls accessed by a one-pot nucleophilic aromatic substitution and sigmatropic rearrangement reaction cascade under mild conditions
- Author
-
Dong-Dong Liang, Shuo Tong, Shen-Yi Guo, and Mei-Xiang Wang
- Subjects
010405 organic chemistry ,Carbazole ,Organic Chemistry ,Sigmatropic reaction ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Medicinal chemistry ,0104 chemical sciences ,chemistry.chemical_compound ,chemistry ,Nucleophilic aromatic substitution ,Drug Discovery ,Rearrangement reaction - Abstract
A practical synthetic method has been developed for polyfunctionalized biaryls based on a facile one-pot nucleophilic aromatic substitution (SNAr) reaction and [5,5]- or [3,3]-sigmatropic rearrangement reaction cascade. Under mild basic conditions, N-arylhydroxylamines reacted with o-activated fluoro (het)arenes to form N,O-diarylhydroxylamine intermediates which underwent spontaneously selective [5,5]-sigmatropic rearrangement reaction to produce diverse functionalized 4-amino-4′-hydroxy-1,1′-biaryls. A sequential SNAr reaction and [3,3]-sigmatropic rearrangement took place between N-arylhydroxylamines and 2-fluoropyridine derivatives or 4-fluorobenzonitrile to afford functionalized 2-amino-2′-hydroxy-1,1′-biaryls. As invaluable and unique building blocks, the resulting biaryls were applied in the straightforward synthesis of N2,O2-coronarene, carbazole, aza- and diaza carbazole derivatives.
- Published
- 2021
24. IRF3 is an important molecule in the UII/UT system and mediates immune inflammatory injury in acute liver failure
- Author
-
Liang-ming Liu, Tong Zhu, Huan Zhong, Dong-yu Liang, Wen-juan Tu, De-Yong Gao, Xiao-ting Wang, and Zhi-li Tan
- Subjects
Male ,0301 basic medicine ,Lipopolysaccharide ,viruses ,Galactosamine ,p38 Mitogen-Activated Protein Kinases ,Receptors, G-Protein-Coupled ,Rats, Sprague-Dawley ,Mice ,chemistry.chemical_compound ,Interferon gamma ,RNA, Small Interfering ,Liver injury ,Mice, Inbred BALB C ,Research Paper: Immunology ,urotensin II ,Oncology ,Immunology and Microbiology Section ,Tumor necrosis factor alpha ,medicine.symptom ,medicine.drug ,medicine.medical_specialty ,Kupffer Cells ,Urotensins ,Active Transport, Cell Nucleus ,Inflammation ,Adenoviridae ,Interferon-gamma ,03 medical and health sciences ,immune-mediated inflammation ,Internal medicine ,medicine ,Animals ,Immune response ,Interleukin-6 ,business.industry ,Immunity ,Transcription Factor RelA ,acute liver failure ,Interferon-beta ,IRF3 ,Liver Failure, Acute ,medicine.disease ,Peptide Fragments ,Rats ,030104 developmental biology ,Endocrinology ,chemistry ,Interferon Regulatory Factor-3 ,business ,Urotensin-II ,Interferon regulatory factors - Abstract
The urotensin II/urotensin receptor (UII/UT) system can mediate inflammatory liver injury in acute liver failure (ALF); however; the related mechanism is not clear. In this study, we confirmed that lipopolysaccharide/D-galactosamine (LPS/D-GalN) induced up-regulation of liver interferon regulatory factor 3 (IRF3) in ALF mice, whereas the UT antagonist urantide inhibited the up-regulated liver IRF3. LPS stimulation induced IRF3 transcription and nuclear translocation and promoted the secretion of interleukin-6 (IL-6), interferon (IFN)-β, and IFN-γ in Kupffer cells (KCs); these effects in LPS-stimulated KCs were inhibited by urantide. Knockdown of IRF3 using an adenovirus expressing an IRF3 shRNA inhibited IFN-β transcription and secretion as well as tumor necrosis factor (TNF)-α and IL-1β secretion from LPS-stimulated KCs; additionally, IL-10 transcription and secretion were promoted in response to LPS. However, LPS-stimulated TNF-α and IL-1β mRNA was not affected in the KCs. The IRF3 shRNA also did not have a significant effect on the NF-κB p65 subunit and p38MAPK protein phosphorylation levels in the nuclei of LPS-stimulated KCs. Therefore, IRF3 expression and activation depended on the signal transduction of the UII/UT system, and played important roles in UII/UT-mediated immune inflammatory injury in the liver but did not affect NF-κB and p38 MAPK activity.
- Published
- 2016
25. Characterization of the Cd(II) and nitrate removal by bacterium Acinetobacter sp. SZ28 under different electron donor conditions
- Author
-
Jun Feng Su, Lei He, Xue Chen Bai, Chun yu Gao, Dong hui Liang, and Ting lin Huang
- Subjects
Denitrification ,Health, Toxicology and Mutagenesis ,Iron ,chemistry.chemical_element ,Electron donor ,Electrons ,Manganese ,010501 environmental sciences ,Nitrate reductase ,01 natural sciences ,Nitrate Reductase ,Water Purification ,chemistry.chemical_compound ,Adsorption ,Nitrate ,Bacterial Proteins ,X-Ray Diffraction ,Environmental Chemistry ,0105 earth and related environmental sciences ,Cadmium ,Nitrates ,Acinetobacter ,General Medicine ,Pollution ,Nitrogen ,chemistry ,Microscopy, Electron, Scanning ,Water Pollutants, Chemical ,Nuclear chemistry - Abstract
In this study, zero-valent iron (ZVI), nanoscale zero-valent iron (nZVI), Fe(II), and Mn(II) were investigated for their effects on mixotrophic denitrification coupled with cadmium (Cd(II)) adsorption process by Acinetobacter sp. SZ28. The removal rates of nitrate were 0.228 mg L−1 h−1 (ZVI), 0.133 mg L−1 h−1 (nZVI), 0.309 mg L−1 h−1 (Fe(II)) and 0.234 mg L−1 h−1 (Mn(II)), respectively. The Cd(II) removal efficiencies were 97.23% (ZVI), 95.79% (nZVI), 80.63% (Fe(II)), and 84.58% (Mn(II)), respectively. Meteorological chromatography analysis indicated that the characteristics of gas composition were different under different electron donor conditions. Moreover, characterization of bacterial metabolites produced by strain SZ28 under different conditions was analyzed. Sequence amplification identified the presence of the nitrate reductase gene (napA) and Mn(II)-oxide gene (cumA) in strain SZ28. The results of XRD and SEM indicated that ZVI, nZVI, Fe(II), and Mn(II) were oxidized into corresponding oxides. XPS spectra indicated that the Cd(II) was adsorbed onto biogenic precipitation.
- Published
- 2018
26. HOXB9 inhibits proliferation in gastric carcinoma cells via suppression of phosphorylated-Akt and NF-κB-dependent Snail expression
- Author
-
Qing Chang, Dong-Yu Liang, Changyu He, Luyi Li, Rong Yang, Qingqing Yi, Qinghua Wu, Li Zhang, Shuang Sha, Boshun Wan, and Junfeng Shi
- Subjects
Mice, Nude ,Apoptosis ,Snail ,Protein Serine-Threonine Kinases ,Real-Time Polymerase Chain Reaction ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Stomach Neoplasms ,biology.animal ,Cell Line, Tumor ,Medicine ,Animals ,Humans ,Gene ,Transcription factor ,Cell Proliferation ,Homeodomain Proteins ,Gene knockdown ,Hepatology ,biology ,Cadherin ,business.industry ,Gastroenterology ,NF-κB ,Transfection ,Cadherins ,Gene Expression Regulation, Neoplastic ,chemistry ,030220 oncology & carcinogenesis ,Cancer research ,030211 gastroenterology & hepatology ,Snail Family Transcription Factors ,business ,Signal Transduction - Abstract
Background HOXB9 is a homeobox transcription factor which plays an important role in carcinoma development. This protein has been shown to inhibit cancer cell proliferation. However, the mechanisms that underpin HOXB9-mediated inhibition of cellular proliferation remain to be elucidated. Methods In this study, two gastric cancer cell lines, SGC7901 and MKN45, were transfected with plasmids pLVX-HOXB9 and shHOXB9. These transfections resulted in the over-expression of the HOXB9 gene in the SGC7901/HOXB9 cells and knockdown of the HOXB9 gene in the MKN45/shHOXB9 cells. Results Over-expression of the HOXB9 gene in the SGC7901/HOXB9 cells caused an increase in the apoptotic rate and a concomitant reduction in metastatic ability compared with the knocked-down MKN45/shHOXB9 cells. Moreover, a reduction in the expression of the phosphorylated-Akt protein was observed in the SGC7901/HOXB9 cells, while an increase in expression of the same protein was observed in the MKN45/shHOXB9 cells. We also observed that HOXB9 mediated a reduction in both NF-κB and N-cadherin and Snail protein expression. Conversely, HOXB9 caused an increase in the expression of E-cadherin. Conclusions In summary, this study reports that HOXB9 can suppress both phosphorylated-Akt expression and NF-κB activity. The latter phenomenon affects Snail protein expression and the inhibition of gastric carcinoma proliferation.
- Published
- 2018
27. Nod2–Rip2 Signaling Contributes to Intestinal Injury Induced by Muramyl Dipeptide Via Oligopeptide Transporter in Rats
- Author
-
Hong-ze Zhang, Sheng-xia Wan, Jingquan Liu, Guoguang Ma, Zijun YinTao, Xiaoli Lou, Bin Shi, Yanqiang Hou, Dong-Yu Liang, and Wan-hua Yang
- Subjects
Male ,Physiology ,Nod2 Signaling Adaptor Protein ,Inflammation ,Pharmacology ,Peptide Transporter 1 ,Rats, Sprague-Dawley ,Jejunum ,chemistry.chemical_compound ,Receptor-Interacting Protein Serine-Threonine Kinase 2 ,NOD2 ,parasitic diseases ,medicine ,Animals ,Intestinal Mucosa ,Oligopeptide ,Dipeptide ,Symporters ,biology ,Glycylglycine ,Chemistry ,Peptide transporter 1 ,NF-kappa B ,Gastroenterology ,Enteritis ,Small intestine ,body regions ,medicine.anatomical_structure ,Biochemistry ,biology.protein ,Cytokines ,Inflammation Mediators ,medicine.symptom ,Acetylmuramyl-Alanyl-Isoglutamine ,Muramyl dipeptide ,Signal Transduction - Abstract
PepT1 can transport bacterial oligopeptide products and induce intestinal inflammation. Our aim was to investigate the mechanism of the small intestine injury induced by bacterial oligopeptide product muramyl dipeptide (MDP) which is transported by PepT1. We perfused the jejunum with a solution with or without MDP, or with a solution of MDP + Gly-Gly and explored the degree of inflammation to determine the role of PepT1–Nod2 signaling pathway in small intestine mucosa. MDP perfusion induced inflammatory cell accumulation and intestinal damage, accompanied by an increase in mucosal Nod2 and Rip2 transcript expression. NFκB activity and inflammatory cytokine expression, including serum levels of TNF-α, IL-1β, and IL-6, increased in the MDP group compared to the controls; these effects were reversed by perfusion of the nutritional dipeptide Gly-Gly. MDP can be transported through PepT1, causing inflammatory damage in the rat small intestine. Nod2–Rip2–NFκB signaling involved in the small intestinal inflammatory injury caused by MDP which is transported through PepT1.
- Published
- 2015
28. Terpenoids with alpha-glucosidase inhibitory activity from the submerged culture of Inonotus obliquus
- Author
-
Lin-Yan Zhang, Dong-E Liang, Xia Zhang, Zha-Jun Zhan, Hai-Bo Bai, Lie-Feng Ma, Wei-Guang Shan, and You-Min Ying
- Subjects
Stereochemistry ,Plant Science ,Fungus ,Horticulture ,Inhibitory postsynaptic potential ,Biochemistry ,Lanosterol ,chemistry.chemical_compound ,medicine ,Glycoside Hydrolase Inhibitors ,Nuclear Magnetic Resonance, Biomolecular ,Molecular Biology ,Acarbose ,Inotodiol ,Mushroom ,Molecular Structure ,biology ,alpha-Glucosidases ,General Medicine ,biology.organism_classification ,Terpenoid ,chemistry ,Alpha-glucosidase ,biology.protein ,Inonotus obliquus ,Agaricales ,Heterocyclic Compounds, 3-Ring ,medicine.drug - Abstract
Lanostane-type triterpenoids, inotolactones A and B, a drimane-type sesquiterpenoid, inotolactone C, and five known terpenoids 6β-hydroxy-trans-dihydroconfertifolin, inotodiol, 3β,22-dihydroxyanosta-7,9(11),24-triene, 3β-hydroxycinnamolide, and 17-hydroxy-ent-atisan-19-oic acid, were isolated from the submerged culture of chaga mushroom, Inonotus obliquus. Their structures were characterized by spectroscopic methods, including MS and NMR (1D and 2D) spectroscopic techniques. Inotolactones A and B, examples of lanostane-type triterpenoids bearing α,β-dimethyl, α,β-unsaturated δ-lactone side chains, exhibited more potent alpha-glucosidase inhibitory activities than the positive control acarbose. This finding might be related to the anti-hyperglycemic properties of the fungus and to its popular role as a diabetes treatment. In addition, a drimane-type sesquiterpenoid and an atisane-type diterpenoid were isolated from I. obliquus.
- Published
- 2014
29. A rhodamine–quinoline type molecular switch as a highly selective sequential sensor for Al3+and F−in aqueous solution
- Author
-
Yongsheng Mi, Dong-Ming Liang, Xubiao Luo, Yating Chen, and Jiannan Xiang
- Subjects
Molecular switch ,Aqueous solution ,General Chemical Engineering ,Metal ions in aqueous solution ,Inorganic chemistry ,Quinoline ,Analytical chemistry ,General Chemistry ,Job plot ,Rhodamine ,chemistry.chemical_compound ,chemistry ,Titration ,Naked eye - Abstract
A novel optical rhodamine–quinoline type chemosensor (REQ) was successfully synthesized and characterized as a reversible molecular switch. It displayed high selectivity toward Al3+ among 19 metal ions and the resultant complex [REQ–Al3+] acted as a sequential chemosensor toward F− among 16 anions in neutral aqueous media (ethanol–water, v/v = 3/7, Tris–HNO3, 20 mM, pH 7.0). Moreover, detecting fluoride at concentrations below the maximum contaminant level in drinking water defined by the U. S. Environmental Protection Agency (4.0 mg L−1, 211 μM) could be achieved with a detection limit (LOD) estimated to be down to 8.0 μM. The reversible ring-open mechanism of the rhodamine spirolactam induced by Al3+ binding and the 1 : 1 stoichiometric structure between REQ and Al3+ were adequately supported by Job plot evaluation, optical titration, and 1H NMR study. Finally, it could be conveniently used for the determination of Al3+ and F− with the “naked eye” due to the obvious color change of the testing solution, showing its potential practical application.
- Published
- 2014
30. A reversible switch as highly selective sequential chemosensor for Al3+ cation followed by F− anion
- Author
-
Dong-Ming Liang, Yongsheng Mi, Yating Chen, Qiufen Xie, Shu Long, Wenping Zhu, Zhong Cao, and Jiannan Xiang
- Subjects
Detection limit ,Metal ions in aqueous solution ,Inorganic chemistry ,Metals and Alloys ,Condensed Matter Physics ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Job plot ,Rhodamine ,chemistry.chemical_compound ,chemistry ,Materials Chemistry ,Proton NMR ,Titration ,Naked eye ,Electrical and Electronic Engineering ,Instrumentation ,Fluoride - Abstract
A novel optical Rhodamine-Furan type chemosensor (RF) was easily synthesized and characterized as a revisable switch. It dispalyed high selectivity toward Al 3+ among 19 metal ions and the resultant complex [RF–Al 3+ ] as a sequential chemosensor toward F − among 16 anions in neutral aqueous media (ethanol/water, v/v = 2/3, Tris–HNO 3 , 10 mM, pH 7.0). Moreover, detecting fluoride at concentration below the maximum contaminant level in drinking water defined by the U.S. Environmental Protection Agency (4.0 mg L −1 , 211 μM) could be achieved with a detection limit (LOD) estimated down to 1.6 μM. Both sensors were very easily to construct and only required simple synthesis. The reversible ring-open mechanism of the rhodamine spirolactam induced by Al 3+ binding and the 1:1 stoichiometric structure between RF and Al 3+ were adequately supported by job plot evaluation, optical titration, and 1 H NMR study. Finally, it could be conveniently used for the determination of fluorides in dental products such as toothpastes with “naked eye” due to the obvious color change of testing solution, showing its potentially practical application.
- Published
- 2014
31. N-Bearing Furanone Derivatives from an Endophytic Fungus inHuperzia serrata
- Author
-
Wei-Guang Shan, You-Min Ying, Dong-E Liang, Jian-Wei Wang, Jia-Gui Xiang, Zha-Jun Zhan, and Li-She Gan
- Subjects
biology ,Stereochemistry ,Organic Chemistry ,Absolute configuration ,Huperzia serrata ,Endophytic fungus ,biology.organism_classification ,Biochemistry ,Endophyte ,Catalysis ,Inorganic Chemistry ,Peyronellaea sp ,Polyketide ,chemistry.chemical_compound ,Biosynthesis ,chemistry ,Drug Discovery ,Pyridine ,Physical and Theoretical Chemistry - Abstract
Two new polyketide derivatives, huaspenones C and D (1 and 2, resp.), were isolated from the cultures of an endophytic fungus Peyronellaea sp. HS-12, derived from the stems of Huperzia serrata. They share N-bearing furan-3(2H)-one backbone, and 2 has an unprecedented furo[3,2-c]pyridine skeleton. Their structures including the absolute configuration were elucidated by extensive spectroscopic analysis combined with quantum-chemical calculations. (2E,4E)-6-hydroxy-2-methylocta-2,4-dienoic acid (3), a key intermediate of the biosynthesis of 1 and 2, was also obtained from the endophyte.
- Published
- 2013
32. An Experimental and Numerical Hybrid Technique for Analysis the Fracture Behaviors in Natural Fiber Reinforced Composites
- Author
-
Min Shen, Dong Chao Liang, Yan Li Duan, and Yang Liu
- Subjects
Polypropylene ,chemistry.chemical_compound ,Digital image correlation ,Toughness ,Materials science ,Ductile materials ,chemistry ,Fracture (geology) ,General Medicine ,Deformation (engineering) ,Composite material ,Natural fiber ,Displacement (vector) - Abstract
Natural fiber reinforced composites have become excellent candidates for automotive applications and civil engineering facilities. The design procedures will require precisely evaluating the toughness of the materials, which demand accurate measurements of strain or displacement that are difficult to obtain for ductile materials. The primary objectives of this paper were to develop reliable experimental approaches which can effectively evaluate the fracture behaviors of random short natural fiber reinforced polypropylene (PP) composites. The digital image correlation method (DICM) was applied to compute the deformation fields around the crack tip of a compact-tension specimen in a process of loading. An experimental and numerical hybrid method was used to describe the fracture behaviors of the composites. The relationship between the micro-structure of random fibers and the macroscopic mechanical properties of the composites can also be understood.
- Published
- 2013
33. Numerical Study on Effects of Interphase in Natural Short Fiber Reinforced Polymer Composite Based on Cohesive Zone Model
- Author
-
Yan Li Duan, Yang Liu, Min Shen, and Dong Chao Liang
- Subjects
Polypropylene ,Cohesive zone model ,chemistry.chemical_compound ,Materials science ,Computer simulation ,chemistry ,Composite number ,Interphase ,General Medicine ,Fiber ,Fibre-reinforced plastic ,Composite material - Abstract
Natural short fiber reinforced polypropylene (PP) composite has great significance both in commercial and environmental and is widely used in motor industry. Its local inhomogeneity and interphase both affect the macroscopic properties of the composite. These phenomena are still difficult to observe and study accurately in the experiment. A cohesive zone model (CZM) based numerical simulation method is presented in this paper. The three-phase (matrix-interphase-fiber) model considering some different factors was developed to study the effects of interphase parameters on the mechanical properties of the composite.
- Published
- 2013
34. Porphyromonas gingivalis infected macrophages upregulate CD36 expression via ERK/NF-κB pathway
- Author
-
Yilong Zhou, Feng Liu, Baoxue Ge, Dong-Yu Liang, Xiao-Li He, Jianxia Chen, and Li-Jun Luo
- Subjects
0301 basic medicine ,MAPK/ERK pathway ,CD36 Antigens ,MAP Kinase Signaling System ,CD36 ,Models, Biological ,03 medical and health sciences ,chemistry.chemical_compound ,Transactivation ,Mice ,Interferon ,parasitic diseases ,medicine ,Bacteroidaceae Infections ,Animals ,Humans ,Scavenger receptor ,Promoter Regions, Genetic ,Porphyromonas gingivalis ,Foam cell ,biology ,NF-kappa B ,hemic and immune systems ,NF-κB ,Cell Biology ,biology.organism_classification ,Cell biology ,Up-Regulation ,Protein Subunits ,030104 developmental biology ,HEK293 Cells ,RAW 264.7 Cells ,chemistry ,Immunology ,biology.protein ,Macrophages, Peritoneal ,Female ,circulatory and respiratory physiology ,medicine.drug ,Foam Cells ,Protein Binding - Abstract
CD36, a scavenger receptor, plays an important role in the progression of atherosclerosis through its interaction with oxidized low-density lipoprotein (ox-LDL). Porphyromonas gingivalis (P. gingivalis, Pg) has been shown to promote macrophage-derived foam cell formation by affecting the expression of CD36. However, the regulatory role of CD36 in macrophages infected with Pg remains largely unknown. Therefore, the aim of the present study was to explore the molecular mechanism of Pg induced CD36 expression in macrophages. Our results showed that Pg promoted ox-LDL uptake by macrophages and the formation of foam cells. Pg infection increased CD36 mRNA and protein levels in ox-LDL-untreated macrophages. Moreover, small interferon RNA (siRNA) targeting CD36 significantly reduced foam cell formation induced by Pg. Additionally, Pg stimulated nuclear translocation of p65, which directly bound to the promoters of CD36 to facilitate its transcription. Inhibition of p65, NF-κB or ERK1/2 blocked Pg-induced CD36 production; whereas, overexpression of NF-κB subunits p65 and p50 upregulated CD36. Furthermore, Ras inhibitors significantly attenuated ERK1/2 activation and CD36 expression. Taken together, the data indicated that stimulation of the ERK/NF-κB pathway by Pg led to transactivation of the CD36 promoters, thereby upregulating CD36 expression in the infected macrophages. These findings may help design new treatment strategies in atherosclerosis.
- Published
- 2016
35. Roscovitine Targets, Protein Kinases and Pyridoxal Kinase
- Author
-
Olivier Lozach, Laurent Meijer, Sophie Schmitt, Flavio Meggio, Christoph Schächtele, Yongqin Wan, Amancio Carnero, Lin Tang, Frank Totzke, Stéphane Bach, Nathanael S. Gray, Stephen P. Coburn, Jens Reinhardt, Marcel Koken, Jean-Francois Dierick, Tao Jiang, Marie Knockaert, Lorenzo A. Pinna, Hervé Galons, Dong-Cai Liang, Blandine Baratte, Andrea S. Lerman, Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Mer et santé (MS), Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Molécules et cibles thérapeutiques (MCT), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Indiana University - Purdue University Indianapolis (IUPUI), Indiana University System, Chinese Academy of Sciences [Beijing] (CAS), Laboratoire de Chimie Organique 2 Glycochimie (LCO2), Méthodologie de synthèse et molécules bioactives (MSMB), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Università degli Studi di Padova = University of Padua (Unipd), Centro Nacional de Investigaciones Oncologicas / Spanish National Cancer Research Centre [Madrid, Espagne] (CNIO), Genomics Institute of the Novartis Research Foundation, and Novartis Research Foundation
- Subjects
Models, Molecular ,CDK ,[SDV]Life Sciences [q-bio] ,Molecular Conformation ,[CHIM.THER]Chemical Sciences/Medicinal Chemistry ,pharmacological selectivity ,Biochemistry ,Chromatography, Affinity ,CLK1 ,Mice ,chemistry.chemical_compound ,Adenosine Triphosphate ,0302 clinical medicine ,Tissue Distribution ,Pyridoxal Kinase ,Cells, Cultured ,Mice, Knockout ,chemistry.chemical_classification ,0303 health sciences ,Molecular Structure ,biology ,Kinase ,Cell Cycle ,Pyridoxal kinase ,3. Good health ,Cell biology ,cyclin-dependent kinase ,Pyridoxal Phosphate ,030220 oncology & carcinogenesis ,Protein kinase inhibitor ,Phosphorylation ,Pyridoxal ,Cell Survival ,Molecular Sequence Data ,Affinity chromatography ,03 medical and health sciences ,Cyclin-dependent kinase ,Roscovitine ,Animals ,Humans ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Amino Acid Sequence ,Protein Kinase Inhibitors ,Molecular Biology ,Seliciclib ,030304 developmental biology ,Cyclin-dependent kinase 2 ,Cell Biology ,Fibroblasts ,Protein Structure, Tertiary ,Rats ,Enzyme ,chemistry ,Purines ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,biology.protein ,Protein Kinases - Abstract
International audience; Roscovitine (CYC202) is often referred to as a "selective inhibitor of cyclin-dependent kinases." Besides its use as a biological tool in cell cycle, neuronal functions, and apoptosis studies, it is currently evaluated as a potential drug to treat cancers, neurodegenerative diseases, viral infections, and glomerulonephritis. We have investigated the selectivity of (R)-roscovitine using three different methods: 1) testing on a wide panel of purified kinases that, along with previously published data, now reaches 151 kinases; 2) identifying roscovitine-binding proteins from various tissue and cell types following their affinity chromatography purification on immobilized roscovitine; 3) investigating the effects of roscovitine on cells deprived of one of its targets, CDK2. Altogether, the results show that (R)-roscovitine is rather selective for CDKs, in fact most kinases are not affected. However, it binds an unexpected, non-protein kinase target, pyridoxal kinase, the enzyme responsible for phosphorylation and activation of vitamin B 6. These results could help in interpreting the cellular actions of (R)-roscovitine but also in guiding the synthesis of more selective roscovitine analogs.
- Published
- 2005
36. Crystal structure studies on rubrerythrin: enzymatic activity in relation to the zinc movement
- Author
-
Jun Liao, Wenrui Chang, Dong-Cai Liang, Jiping Zhang, Mei Li, Tao Jiang, Ming-Yih Liu, Lulu Gui, and Jean LeGall
- Subjects
Models, Molecular ,Iron ,chemistry.chemical_element ,Rubrerythrin ,Zinc ,Crystal structure ,Crystallography, X-Ray ,Biochemistry ,Inorganic Chemistry ,Crystal ,Metal ,chemistry.chemical_compound ,Bacterial Proteins ,Molecule ,Desulfovibrio vulgaris ,Pyrophosphatases ,biology ,Rubredoxins ,biology.organism_classification ,Hemerythrin ,Crystallography ,Monomer ,chemistry ,visual_art ,visual_art.visual_art_medium ,Ferredoxins ,Dimerization ,Oxidation-Reduction - Abstract
Rubrerythrin (Rr) is a non-heme iron protein isolated from anaerobic sulfate-reducing bacteria. Rr is a dimeric molecule, each monomer contains a Fe(SCys)4 center in the C-terminal domain and a binuclear metal center in the N-terminal domain. Rr structures with different protein sources and/or preparation procedures have been studied. Two Rr crystal structures have been solved with significant differences in their binuclear metal centers. The first structure, which was obtained from expressed protein under aerobic conditions, has a diiron–oxo center. The second structure, which was obtained from native protein of Desulfovibrio vulgaris under aerobic conditions, has an Fe–Zn center with the zinc position differing from the corresponding iron position in the former structure by approximately 2 A. The crystal structures of Rr isolated from D. vulgaris (Hildenborough, NCIB 8303), the same as the second structured but prepared under anaerobic conditions, are reported in this paper. The binuclear metal center in these structures is an Fe–Zn center. When the crystal was exposed to air, the zinc atom moved gradually, approximately 2 A, accompanied by the entrance of a water molecule (or hydroxyl group) and changes in the binuclear metal center microenvironment. This finding can explain the differences between the two different structures. The results suggest that the zinc movement may be related to the enzymatic activity of Rr. Electronic supplementary material is available if you access this article at http://dx.doi.org/10.1007/s00775-002-0400-0. On that page (frame on the left side), a link takes you directly to the supplementary material.
- Published
- 2003
37. Effects of urotensin-II on cytokines in early acute liver failure in mice
- Author
-
Liang Zhao, Fang-ping Yu, Tong Zhu, Dong-yu Liang, Wen-juan Tu, Liang-ming Liu, and Chang-gen Ye
- Subjects
Lipopolysaccharides ,Male ,medicine.medical_specialty ,Time Factors ,Lipopolysaccharide ,Urotensins ,Interleukin-1beta ,Priming (immunology) ,Galactosamine ,Receptors, G-Protein-Coupled ,Pathogenesis ,chemistry.chemical_compound ,Downregulation and upregulation ,Internal medicine ,medicine ,Animals ,Receptor ,Mice, Inbred BALB C ,business.industry ,Tumor Necrosis Factor-alpha ,digestive, oral, and skin physiology ,Gastroenterology ,Interleukin ,General Medicine ,Liver Failure, Acute ,Basic Study ,Peptide Fragments ,Disease Models, Animal ,Endocrinology ,chemistry ,Liver ,Tumor necrosis factor alpha ,Inflammation Mediators ,business ,Urotensin-II ,Signal Transduction - Abstract
AIM: To investigate urotensin-II (UII) and its effects on tumor necrosis factor (TNF)-α and interleukin (IL)-1β in early acute liver failure (ALF). METHODS: We investigated the time-dependent alteration in UII levels and its effects on TNF-α and IL-1β in liver and blood in the early stage of lipopolysaccharide/D-galactosamine-induced ALF. RESULTS: After lipopolysaccharide/D-galactosamine challenge, UII rose very rapidly and reached a maximal level 0.5 h, and the level remained significantly elevated after 2 h (P < 0.05). Six hours after challenge, UII began to degrade, but remained higher than at 0 h (P < 0.05). Pretreatment with urantide, an inhibitor of the UII receptor, suppressed the degree of UII increase in liver and blood at 6 h after challenge (P < 0.05 vs paired controls). In addition, liver and blood TNF-α increased from 1 to 6 h, and reached a peak at 1 and 2 h, respectively; however, IL-1β did not rise until 6 h after challenge. Urantide pretreatment inhibited the degree of TNF-α and IL-1β increase following downregulation of UII post-challenge (all P < 0.05). CONCLUSION: UII plays a role in the pathogenesis and priming of ALF by triggering an inflammatory cascade and driving the early release of cytokines in mice.
- Published
- 2014
38. A novel Alu-based real-time PCR method for the quantitative detection of plasma circulating cell-free DNA: sensitivity and specificity for the diagnosis of myocardial infarction
- Author
-
Xiaoli Lou, Yanqiang Hou, Hongwei Chen, Dong-Yu Liang, Shanyuan Ma, Liang Peng, and Lurong Zhang
- Subjects
Adult ,Male ,Pathology ,medicine.medical_specialty ,Alu ,Myocardial Infarction ,Biology ,Real-Time Polymerase Chain Reaction ,Sensitivity and Specificity ,cell-free DNA ,chemistry.chemical_compound ,Alu Elements ,Lactate dehydrogenase ,Troponin I ,Genetics ,medicine ,Humans ,Aged ,Aged, 80 and over ,Receiver operating characteristic ,Reproducibility of Results ,General Medicine ,DNA ,Articles ,Middle Aged ,Molecular biology ,Circulating Cell-Free DNA ,Real-time polymerase chain reaction ,Cell-free fetal DNA ,chemistry ,ROC Curve ,Case-Control Studies ,Human genome ,Female ,Myocardial infarction diagnosis ,real-time PCR - Abstract
In the present study, we aimed to develop and validate a rapid and sensitive, Alu-based real-time PCR method for the detection of circulating cell-free DNA (cfDNA). This method targeted repetitive elements of the Alu reduplicative elements in the human genome, followed by signal amplification using fluorescence quantification. Standard Alu-puc57 vectors were constructed and 5 pairs of specific primers were designed. Valuation was conducted concerning linearity, variation and recovery. We found 5 linear responses (R1–5=0.998–0.999). The average intra- and inter-assay coefficients of variance were 12.98 and 10.75%, respectively. The recovery was 82.33–114.01%, with a mean recovery index of 101.26%. This Alu-based assay was reliable, accurate and sensitive for the quantitative detection of cfDNA. Plasma from normal controls and patients with myocardial infarction (MI) were analyzed, and the baseline levels of cfDNA were higher in the MI group. The area under the receiver operating characteristic (ROC) curve for Alu1, Alu2, Alu3, Alu4, Alu5 and Alu (Alu1 + Alu2 + Alu3 + Alu4 + Alu5) was 0.887, 0.758, 0.857, 0.940, 0.968 and 0.933, respectively. The optimal cut-off value for Alu1, Alu2, Alu3, Alu4, Alu5 and Alu to predict MI was 3.71, 1.93, 0.22, 3.73, 6.13 and 6.40 log copies/ml. We demonstrate that this new method is a reliable, accurate and sensitive method for the quantitative detection of cfDNA and that it is useful for studying the regulation of cfDNA in certain pathological conditions. Alu4, Alu5 and Alu showed better sensitivity and specificity for the diagnosis of MI compared with cardiac troponin I (cTnI), creatine kinase MB (CK-MB) isoenzyme and lactate dehydrogenase (LDH). Alu5 had the best prognostic ability.
- Published
- 2014
39. Molecular replacement studies on crystal structure of allophycocyanin from red algaePorphyra yezoensis
- Author
-
Jin-Yu Liu, Tao Jiang, Wenrui Chang, Jiping Zhang, and Dong-Cai Liang
- Subjects
Allophycocyanin ,biology ,Mineralogy ,Crystal structure ,Red algae ,Chromophore ,biology.organism_classification ,General Biochemistry, Genetics and Molecular Biology ,chemistry.chemical_compound ,Crystallography ,Reflection (mathematics) ,Monomer ,chemistry ,Molecular replacement ,Porphyra yezoensis ,General Agricultural and Biological Sciences ,General Environmental Science - Abstract
Using the crystal structure of allophycocyanin from cyanobacteriumSpirulina platensis (APC-SP) as a search model, the crystal structure of allophycocyanin from red algaePorphyra yezoensis (APC-PY) has been studied by molecular replacement methods. The APC-PY crystals (Form 3) belong to the space group of R32, cell dimensions a =b = 10.53 nm,c = 18.94 nm, alpha= beta = 90 degrees , gamma= 120 degrees ; there is one alphabeta monomer in each crystallographic asymmetric unit in the cell. The translation function search gave a unique peak with a correlation coefficient (Cc) of 67.0% and an R-factor of 36.1 % for reflection data from 1.0 to 0.4 nm. Using the results by molecular replacement, the initial model of APC-PY was built, and the coincidence of the chromophore in APC-PY initial model with its2F (o)- F(c) OMIT map further confirms the results by molecular replacement.
- Published
- 2000
40. Structure of desheptapeptide (B24–B30) insulin in a new crystal form
- Author
-
Jiping Zhang, Dong-Cai Liang, Sujin Bao, and Wenrui Chang
- Subjects
Chemistry ,Dimer ,Resolution (electron density) ,Mineralogy ,Crystal structure ,General Biochemistry, Genetics and Molecular Biology ,law.invention ,Solvent ,Crystal ,Crystallography ,chemistry.chemical_compound ,law ,X-ray crystallography ,Molecule ,Crystallization ,General Agricultural and Biological Sciences ,General Environmental Science - Abstract
The structure of desheptapeptide (B24-B30) insulin (DHPI) in a new crystal form (form B) has been determined and refined to 0.2 nm resolution. The crystals were obtained under the same crystallization condition as previously reported crystal form (form A). The overall structures of the two crystal forms are similar but obvious differences can be observed in crystal packing and local conformation. The crystal structures of the two forms show that the two independent molecules in an asymmetric unit from a DHPI dimer, and the dimer formation buries more than 18.20 and 16.95 nm(2) of solvent accessible surfaces for form A and form B DHPI, respectively, the largest among insulin and insulin analogs ever reported. Close examination at crystal packing shows that the dimer-forming surface of DHPI, namely Surface II, is normally present in the association of insulin and insulin analogs in their crystal structures. The results demonstrate that Surface II is crucially important for the formation of two crystal forms under the same crystallization condition.
- Published
- 1999
41. Structure and function of chromophores in R-phycoerythrin at 1.9 � resolution
- Author
-
Jiping Zhang, Tao Jiang, and Dong-Cai Liang
- Subjects
Electron density ,Phycourobilin ,Resolution (electron density) ,Crystal structure ,Chromophore ,Biochemistry ,chemistry.chemical_compound ,symbols.namesake ,Crystallography ,Protein structure ,Fourier transform ,chemistry ,Structural Biology ,symbols ,Molecule ,Molecular Biology - Abstract
The crystal structure of R-Phycoerythrin (R-PE) from Polysiphonia urceolata has been refined to a resolution of 1.9 A, based on the atomic coordinates of R-PE determined at 2.8 A resolution, through the use of difference Fourier method and steorochemistry parameters restrained refinement with model adjustment according to the electron density map. Crystallographic R-factor of the refined model is 0.195 (Rfree = 0.282) from 8-1.9 A. High resolution structure of R-PE showed precise interactions between the chromophores and protein residues, which explained the spectrum characteristic and function of chromophores. Four chiral atoms of phycourobilin (PUB) were identified as C(4)-S, C(16)-S, C(21)-S, and C(20)-R. In addition to the coupling distances of 19 A to 45 A between the chromophores which were observed and involved in the energy transfer pathway, high resolution structure of R-PE suggested other pathways of energy transfer, such as the ultrashort distance between alpha140a and beta155. It has been proposed that aromatic residues in linker proteins not only influence the conformation of chromophore, but may also bridge chromophores to improve the energy transfer efficiency.
- Published
- 1999
42. Study on the relationship between serum high density lipoprotein and Pi-deficiency syndrome in patients with coronary heart disease
- Author
-
Yun-qin Chen, Xiao-min Li, Zhi-liang Li, and Dong-hui Liang
- Subjects
Adult ,Male ,medicine.medical_specialty ,Blood lipids ,Coronary Disease ,chemistry.chemical_compound ,High-density lipoprotein ,Internal medicine ,medicine ,Humans ,Pharmacology (medical) ,In patient ,Medicine, Chinese Traditional ,skin and connective tissue diseases ,Serum high density lipoprotein ,Aged ,Splenic Diseases ,business.industry ,Metabolic disorder ,Pi deficiency ,General Medicine ,Middle Aged ,Blood Protein Electrophoresis ,medicine.disease ,Coronary heart disease ,Endocrinology ,Complementary and alternative medicine ,chemistry ,Medicine public health ,Female ,lipids (amino acids, peptides, and proteins) ,sense organs ,Lipoproteins, HDL ,business - Abstract
To explore the relationship of Pi-deficiency syndrome in TCM with the change of serum high density lipoprotein (HDL) in blood lipid metabolic disorder.Sixty-eight patients with confirmed coronary heart disease (CHD) were selected for TCM syndrome typing into Pi-deficiency (PD) group and non-Pi-deficiency (NPD) group. Routine blood lipids and serum lipoprotein electrophoretogram (SLPG) were determined in all patients to analyze the total content of HDL and its relative contents of sub-components HDL(1-5), as well as their relation with PD syndrome. Besides, a healthy control group (62 cases) was set up.The level of serum HDL-C was lowered, SLPG abnormality rate increased in the patients with CHD, with total HDL and the relative contents of subcomponent HDL(1) and HDL(3) significantly lower than those in the healthy control group (P0.01). The total HDL, HDL(1) and HDL(3) in the PD group were also lower than those in the NPD group (P0.05, P0.01).Serum HDL and its sub-components showed a definite relation with TCM PD syndrome type, therefore, further exploring the granular specificity of HDL and its sub-components as well as their influence on reverse cholesterol transport (RCT) may hopefully provide clues for developing RCT regulatory Chinese new drugs and for CHD prevention and treatment.
- Published
- 2007
43. Structural analysis of desheptapeptide(B24-B30) insulin by molecular replacement
- Author
-
Sujin Bao, Dong-Cai Liang, Jiping Zhang, Wenrui Chang, and Dian-lin Xie
- Subjects
Diffraction ,medicine.medical_specialty ,Materials science ,Insulin ,medicine.medical_treatment ,Resolution (electron density) ,General Biochemistry, Genetics and Molecular Biology ,chemistry.chemical_compound ,Crystallography ,Monomer ,Endocrinology ,chemistry ,Internal medicine ,medicine ,Molecule ,Molecular replacement ,General Agricultural and Biological Sciences ,General Environmental Science - Abstract
Desheptapeptide (B24-B30) insulin (DHPI), a virtually inactive insulin analog, has been crystallized in space group P2, 2, 2, with two DHPI molecules in an asymmetric unit. The orientatin and positions of the molecules were determind by molecular replacement. and a structural model was built at 0.3 nm resolution. The current model shows that the two DMHI monomers are related by a non-crystallographic 2-fold axis, nearly parallel to the crystallographicc axis. This structural feature complicated the determination of the orientation of the local 2-fold axis, which was later confirmed by analysing the diffraction data of DHPI crystals.
- Published
- 1998
44. Crystal structure of desheptapeptide(B24–B30)insulin at 1.6 Å resolution: Implications for receptor binding
- Author
-
Sujin Bao, Wen-rui Chang, Jiping Zhang, Dian-lin Xie, and Dong-cai Liang
- Subjects
Multidisciplinary ,Stereochemistry ,Relative motion ,Insulin ,medicine.medical_treatment ,Molecular Sequence Data ,Resolution (electron density) ,Plasma protein binding ,Crystal structure ,Biological Sciences ,Crystallography, X-Ray ,Structural framework ,Peptide Fragments ,chemistry.chemical_compound ,Models, Chemical ,chemistry ,Functional group ,medicine ,Structured model ,Protein Binding - Abstract
The crystal structure of desheptapeptide (B24–B30) insulin (DHPI), a virtually inactive analog of insulin, was determined at 1.6 Å resolution. In the refined structure model, DHPI retains three α-helices (A1–A8, A12–A18, and B9–B19) as its structural framework, while great conformational changes occur in the N and C termini of B-chain. The β-turn, which lies in B20–B30 in insulin and insulin analogs with high potency, no longer exists in DHPI. Relative motion is observed among the three α-helices, each as a rigid functional group. In contrast, a region covering B5–B6 and A6–A11 exhibits a relatively stable conformation. We interpret our results as identifying: ( i ) the importance of β-turn in determining the receptor-binding potency of insulin and ( ii ) a leading role of Phe B24 in maintaining the β-turn structure.
- Published
- 1997
45. RecOR complex including RecR N-N dimer and RecO monomer displays a high affinity for ssDNA
- Author
-
Pu Gao, Shun Liu, Ang Gao, Xiao-Xue Yan, Xiao-Min An, Dong-Cai Liang, Qun Tang, and Yan-Ping Liu
- Subjects
Models, Molecular ,Stereochemistry ,Dimer ,Lysine ,Mutant ,DNA, Single-Stranded ,Thermoanaerobacter ,Plasma protein binding ,Biology ,Hydrophobic effect ,chemistry.chemical_compound ,Monomer ,Tetramer ,chemistry ,Biochemistry ,Bacterial Proteins ,Structural Biology ,Genetics ,Binding site ,Protein Multimerization ,Dimerization ,DNA ,Protein Binding - Abstract
RecR is an important recombination mediator protein in the RecFOR pathway. RecR together with RecO and RecF facilitates RecA nucleoprotein filament formation and homologous pairing. Structural and biochemical studies of Thermoanaerobacter tengcongensis RecR (TTERecR) and its series mutants revealed that TTERecR uses the N-N dimer as a basic functional unit to interact with TTERecO monomer. Two TTERecR N-N dimers form a ring-shaped tetramer via an interaction between their C-terminal regions. The tetramer is a result of crystallization only. Hydrophobic interactions between the entire helix-hairpin-helix domains within the N-terminal regions of two TTERecR monomers are necessary for formation of a RecR functional N-N dimer. The TTERecR N-N dimer conformation also affects formation of a hydrophobic patch, which creates a binding site for TTERecO in the TTERecR Toprim domain. In addition, we demonstrate that TTERecR does not bind single-stranded DNA (ssDNA) and binds double-stranded DNA very weakly, whereas TTERecOR complex can stably bind DNA, with a higher affinity for ssDNA than double-stranded DNA. Based on these results, we propose an interaction model for the RecOR:ssDNA complex.
- Published
- 2012
46. High-resolution structures of AidH complexes provide insights into a novel catalytic mechanism for N-acyl homoserine lactonase
- Author
-
Ang Gao, Xiao Xue Yan, Gui Ying Mei, Qun Tang, Shun Liu, Xiao Min An, Hui Wen, Li-Qun Zhang, Ping Wang, Yan Ping Liu, and Dong Cai Liang
- Subjects
Cell signaling ,Stereochemistry ,Homoserine ,Virulence ,Cell Communication ,Ochrobactrum ,medicine.disease_cause ,α/β-hydrolases ,Catalysis ,Enzyme catalysis ,Substrate Specificity ,chemistry.chemical_compound ,Structural Biology ,Catalytic Domain ,Hydrolase ,medicine ,covalent catalysis ,lactonases ,biology ,acid–base catalysis ,Hydrolysis ,food and beverages ,Active site ,quorum sensing ,Pathogenic bacteria ,General Medicine ,biochemical phenomena, metabolism, and nutrition ,Hydrogen-Ion Concentration ,Research Papers ,Quorum sensing ,chemistry ,Biochemistry ,biology.protein ,Mutagenesis, Site-Directed ,Carboxylic Ester Hydrolases - Abstract
Crystal structures of the AHL-lactonase AidH in complex with substrate and product are reported at high resolution and a catalytic mechanism is proposed for the metal-independent AHL-lactonase., Many pathogenic bacteria that infect humans, animals and plants rely on a quorum-sensing (QS) system to produce virulence factors. N-Acyl homoserine lactones (AHLs) are the best-characterized cell–cell communication signals in QS. The concentration of AHL plays a key role in regulating the virulence-gene expression and essential biological functions of pathogenic bacteria. N-Acyl homoserine lactonases (AHL-lactonases) have important functions in decreasing pathogenicity by degrading AHLs. Here, structures of the AHL-lactonase from Ochrobactrum sp. (AidH) in complex with N-hexanoyl homoserine lactone, N-hexanoyl homoserine and N-butanoyl homoserine are reported. The high-resolution structures together with biochemical analyses reveal convincing details of AHL degradation. No metal ion is bound in the active site, which is different from other AHL-lactonases, which have a dual Lewis acid catalysis mechanism. AidH contains a substrate-binding tunnel between the core domain and the cap domain. The conformation of the tunnel entrance varies with the AHL acyl-chain length, which contributes to the binding promiscuity of AHL molecules in the active site. It also supports the biochemical result that AidH is a broad catalytic spectrum AHL-lactonase. Taken together, the present results reveal the catalytic mechanism of the metal-independent AHL-lactonase, which is a typical acid–base covalent catalysis.
- Published
- 2012
47. Influence of the Subtle Structure of Montmorillonite on Zeta-potential of Montmorillonite-water Colloid Systems
- Author
-
Dong Zhen-Liang and Jiang Yin-Shan
- Subjects
chemistry.chemical_compound ,Colloid ,Montmorillonite ,chemistry ,Chemical engineering ,Zeta potential ,Physical and Theoretical Chemistry - Published
- 1994
48. Crystallization and preliminary crystallographic studies of pyridoxal kinase from sheep brain
- Author
-
Chi Kong Lau, Sheng Quan Liu, Tao Jiang, Wenrui Chang, Jiping Zhang, Yun Chung Leung, Minghui Li, Francis Kwok, Dong Cai Liang, and Xiao Min An
- Subjects
chemistry.chemical_classification ,Sheep ,biology ,Protein Conformation ,Stereochemistry ,Brain ,General Medicine ,Metabolism ,Crystallography, X-Ray ,Phosphate ,Pyridoxal kinase ,Cofactor ,Amino acid ,Crystallography ,chemistry.chemical_compound ,Enzyme ,chemistry ,Biochemistry ,Structural Biology ,Sodium citrate ,biology.protein ,Animals ,Crystallization ,Pyridoxal - Abstract
Pyridoxal kinase (ATP:pyridoxal 5'-phosphotransferase; EC 2.7.1.35) is a key enzyme in the transformation of vitamin B(6) to pyridoxal-5'-phosphate. Pyridoxal-5'-phosphate is the crucial cofactor required by numerous enzymes involved in the metabolism of amino acids and the synthesis of many neurotransmitters. Pyridoxal kinase from sheep brain was crystallized in an orthorhombic form using the hanging-drop vapour-diffusion method with sodium citrate as the precipitant. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 59.8, b = 94.4, c = 128.2 A, and diffract to a resolution of 2.1 A. Crystals were transferred into a soaking liquid without citrate and two heavy-atom derivatives were prepared.
- Published
- 2002
49. Crystallization and preliminary crystallographic analysis of manganese superoxide dismutase from Bacillus halodenitrificans
- Author
-
Tschining Chang, Ming-Yih Liu, Dong-Cai Liang, Jiping Zhang, Mei Li, Jun Liao, Jean Le Gall, Wenrui Chang, and Lulu Gui
- Subjects
chemistry.chemical_classification ,biology ,Protein Conformation ,Superoxide Dismutase ,Biophysics ,Bacillus ,Cell Biology ,Crystallography, X-Ray ,Biochemistry ,Manganese Superoxide Dismutase ,Halophile ,Catalysis ,law.invention ,Superoxide dismutase ,Crystallography ,chemistry.chemical_compound ,Enzyme ,Monomer ,chemistry ,law ,biology.protein ,Crystallization ,Molecular Biology ,Bacillus halodenitrificans - Abstract
Manganese superoxide dismutase (GP-MnSOD), a component of the so-called `green protein' (green protein complex) from the facultative anaerobic halodenitrifier Bacillus halodenitrificans , has been crystallized using the hanging-drop vapor diffusion method. Crystals have unit-cell parameters a = b =93.4 A, c =65.0 A, and belong to the space group P4 3 2 1 2. Preliminary analysis indicates there is one monomer in each asymmetric unit. The structural information from this enzyme will enrich our knowledge on its high catalytic activity and its possible role in green protein complex.
- Published
- 2002
50. Structure of HsdS Subunit from Thermoanaerobacter tengcongensis Sheds Lights on Mechanism of Dynamic Opening and Closing of Type I Methyltransferase
- Author
-
Xiao-Min An, Xiao-Xue Yan, Qun Tang, Pu Gao, and Dong-Cai Liang
- Subjects
Macromolecular Assemblies ,DNA, Bacterial ,Models, Molecular ,Methyltransferase ,Stereochemistry ,Protein Conformation ,Protein subunit ,Biophysics ,lcsh:Medicine ,Thermoanaerobacter ,Biology ,Crystallography, X-Ray ,DNA Restriction-Modification Enzymes ,Biochemistry ,chemistry.chemical_compound ,Protein structure ,Bacterial Proteins ,Transferases ,DNA-binding proteins ,Electrophoretic mobility shift assay ,lcsh:Science ,Protein chemistry ,Multidisciplinary ,Enzyme Classes ,lcsh:R ,Proteins ,DNA ,Genomics ,Methyltransferases ,biology.organism_classification ,Enzyme structure ,Enzymes ,body regions ,Nucleic acids ,Protein Subunits ,chemistry ,Enzyme Structure ,Mutation ,lcsh:Q ,Structural Genomics ,DNA modification ,Research Article ,Protein Binding - Abstract
Type I DNA methyltransferases contain one specificity subunit (HsdS) and two modification subunits (HsdM). The electron microscopy model of M.EcoKI-M2S1 methyltransferase shows a reasonable closed state of this clamp-like enzyme, but the structure of the open state is still unclear. The 1.95 A crystal structure of the specificity subunit from Thermoanaerobacter tengcongensis (TTE-HsdS) shows an unreported open form inter-domain orientation of this subunit. Based on the crystal structure of TTE-HsdS and the closed state model of M.EcoKI-M2S1, we constructed a potential open state model of type I methyltransferase. Mutational studies indicated that two α-helices (aa30-59 and aa466-495) of the TTE-HsdM subunit are important inter-subunit interaction sites in the TTE-M2S1 complex. DNA binding assays also highlighted the importance of the C-terminal region of TTE-HsdM for DNA binding by the TTE-M2S1 complex. On the basis of structural analysis, biochemical experiments and previous studies, we propose a dynamic opening and closing mechanism for type I methyltransferase.
- Published
- 2011
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.