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Isovitexin, a marker compound with various pharmacological activities, in Lespedeza cuneata, was analyzed using high performance liquid chromatography coupled with UV (HPLC/UV). There are no previous reports on using L. cuneata as the source material for the quantification of isovitexin. In this study, we developed an optimized method using HPLC-UV analysis, which was validated using various parameters. Our method demonstrated high specificity, and good separation of the chromatographic peak was achieved. Parameters such as linearity (r2>0.9997), precision, and accuracy indicated that our proposed analytical method had good reliability and sensitivity. These results demonstrate the utility and convenience of our method for rapidly quantifying isovitexin in L. cuneata extracts.

The aim of this research was to investigate the industrial application of Anthriscus sylvestris. Antibacterial activities of the n-hexane, methylene chloride (MC), ethyl acetate, and butanol fractions of A. sylvestris were tested against Escherichia coli, Staphylococcus aureus, and Helicobacter pylori. The n-hexane and MC fractions showed a stronger antibacterial activity against S. aureus than other fractions, with an inhibition zone greater than 11 mm in disc assays. At 100 μg·mL−1, all fractions extracted from A. sylvestris scavenged more than 85% of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals (·OH); however, the n-hexane and MC fractions showed the strongest scavenging activity of DPPH and ·OH. In addition, we found that treatment with the n-hexane and MC fractions inhibited the growth of H. pylori and gastric adenocarcinoma cells. Further testing of falcarindiol and deoxypodophyllotoxin from the MC fraction of A. sylvestris revealed the antibacterial effects against E. coli, S. aureus, and H. pylori, suggesting their potential use as antibacterial agents.

We investigated the effect of extracts(50 µg/mL) from 31 folk plants in Ulleung Island on nitric oxide(NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Actinidia arguta (leaf, twig), Hovenia dulcis (leaf), Camellia japonica (leaf, twig), Sambucus sieboldiana var . pendula (leaf, twig) and Fallopia sachalinensis (root) showed significant NO inhibition activity(42%~88% NO inhibition rate). Of them, we selected 3 samples( A. arguta, H. dulcis and C. japonica ) showing more than 50% NO inhibition activity and little effect on cell viability. We found that the extracts suppressed the NO and prostaglandin E 2 (PGE 2 ) produced by LPS-activated RAW264.7 cells in dose-dependent manners. Key words - Actinidia arguta , Hovenia dulcis , Camellia japonica , Nitric oxide, Prostaglandin E 2 * 교신저자(E-mail): jjku@forest.go.kr ⓒ 본 학회지의 저작권은 (사)한국자원식물학회지에 있으며, 이의 무단전재나 복제를 금합니다. 서 언 최근 세계 각국에서는 자국의 자생식물에 대한 전통지식을 기반으로 활용하는 민속식물에 관한 관심이 고조되고 있다. 특히 2010년 일본 나고야에서 개최된 생물다양성협약에서 유전자원과 전통지식의 접근 및 이익분배에 관한 나고야의정서가 채택되는 등, 전통지식을 공유하고, 나아가 민속식물의 유용성 탐색을 통한 소재식물의 발굴 및 개발이 절실히 필요한 실정이다 . 이에 관한 국내의 연구는 경기도 내륙 남부, 경상북도 내륙 및 울릉도, 경상남도 내륙, 강원도 남부, 강원도 북부 등 한반도 전역에서 민속식물 이용 정보를 수집 및 발굴하였다(Choi et al., 2010; Chung et al., 2010; Chung et al., 2011; Im et al., 2011; Jeong et al., 2012; Kim et al., 2012). 따라서 이를 활용한 건강기능 제품 및 의약품분야에 폭넓게 활용 될 수 있는 기능성 소재를 탐색하는 것이 필요한 실정이다 . 기능성 분야 연구는 매우 다양화되고 있는데, 그 중 염증으로 인한 질병을 예방 또는 치료하는 데 목적을 둔 항염증 소재 연구도 한 부분을 차지하고 있다.염증 반응은 외상이나 세균의 침입으로 인해 손상된 조직에 대한 생체 조직의 국소적 방어반응이다 . 염증 반응이 일어나면 여러 가지 염증매개인자들이 만들어지는 데 이로 인하여 통증, 발열, 홍반, 부종 및 기능 상실 등의 증상이 나타난다(Sarkar and Fisher, 2006; Fierro and Serhan, 2001; Park et al., 2007). 대식세포는 숙주의 항상성 유지와 같이 여러 종류의 숙주 반응에 관여하는 것으로 알려져 있으며, nitric oxide(NO), prostaglandin E

The phenolic compounds (quercitrin, isoquercitrin, and 1,5-di-O-caffeoylquinic acid) in chwinamul were determined by high performance liquid chromatography (HPLC). The phenolic compounds were quantified using a reverse-phase column with an elution program of an initial solvent gradient of H2O:ACN = 75:25 for 40 min, then 50:50 for 20 min, and finally 20:80 for 10 min. UV monitoring was at 330 nm. The most abundant phenolic compounds in chwinamul (Saussurea grandifolia stem, Synulus excelsus flower, and Aster pilosus) were quercitrin (18.57 mg·g−1), isoquercitrin (44.67 mg·g−1), and 1,5-di-O-caffeoylquinic acid (78.25 mg·g−1), respectively. The quantitative data and the HPLC method established in the present study could be used for rapid evaluation of the quantities of phenolic compounds in chwinamul.

Five Korean Calanthe species, C. discolor, C. bicolor, C. sieboldii, C. reflexa, and C. aristulifera, were studied using amplified fragment length polymorphism (AFLP) to assess their taxonomic and genetic rela- tionships. Sixteen accessions belonging to five native Calanthe spp. and mutants with yellow tepal and white lip (YW mutants) were studied. We identified 50 putative markers using AFLP analysis. The results of AMOVA showed that genetic variance was higher between species than within species. Genetic dissimilarity when com- pared with the rest of the species was the lowest for individuals of the YW mutants and the highest for indi- viduals of C. reflexa. The mutants clustered outside the major group. Calanthe bicolor clustered with C. discolor, suggesting that its genetic composition is closer to that of C. discolor. Though it is suggested to have originated as a result of natural hybridization between C. sieboldii and C. discolor, introgression is likely to have occurred in the direction of C. discolor based on the data of molecular marker, clustering and genetic dissim- ilarity. Calanthe reflexa and C. aristulifera were genetically the most diverse of the species studied. In conclu- sion, the results showed that there is genetic diversity in Korean Calanthe species, that C. bicolor introgressed in the direction of C. discolor and that the YW mutants are genetically closer to C. sieboldii.