1. Monocarboxylate Transporter-1 Mediates the Protective Effects of Neutral-pH Bicarbonate/Lactate-Buffered Peritoneal Dialysis Fluid on Cell Viability and Apoptosis
- Author
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Tetsu Miyamoto, Yoshikazu Harada, Ryota Serino, Nana Ishimatsu, Hiroto Izumi, Yutaka Otsuji, Masahito Tamura, Yoko Nakano, Akihiro Kuma, and Yumi Furuno
- Subjects
0301 basic medicine ,Small interfering RNA ,Bicarbonate ,030232 urology & nephrology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Medicine ,Viability assay ,Gene knockdown ,TUNEL assay ,biology ,business.industry ,fungi ,Transporter ,Hematology ,Molecular biology ,body regions ,030104 developmental biology ,Monocarboxylate transporter 1 ,nervous system ,Biochemistry ,chemistry ,Nephrology ,Apoptosis ,biology.protein ,business - Abstract
We investigated the effects of bicarbonate/lactate-buffered peritoneal dialysis fluid (B/L-PDF) and lactate-buffered PDF (L-PDF) on cell viability and apoptosis, focusing on monocarboxylate transporters (MCTs). MCT-1 transports lactate into cells. Cell viability and apoptosis of human peritoneal mesothelial cells (HPMCs) were examined by water-soluble tetrazolium salt-1 and TUNEL assays, respectively. The relative number of viable HPMCs was significantly decreased by L-PDF at 48 h (8.8 ± 0.4%) compared with cells cultured in M199, but not by B/L-PDF (66.7 ± 1.1%). Apoptosis was markedly induced by L-PDF at 48 h (69.3 ± 16.2%), but not by B/L-PDF (2.6 ± 0.3%). Knockdown of MCT-1 by small interfering RNA (siRNA) attenuated the L-PDF-induced reduction of viable cells and increased apoptosis compared with control siRNA, but MCT-4 knockdown had no effect. B/L-PDF had lesser effects on cell viability and apoptosis of HPMCs compared with L-PDF. These results suggest that B/L-PDF biocompatibility occurs by avoiding the induction of apoptosis in HPMCs.
- Published
- 2016