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This study was conducted to investigate the effects of chitosan oligosaccharide (COS) supplementation on intestinal development and functions, inflammatory response, antioxidant capacity and the related signaling pathways in broilers aged d 1 to 14. A total of 240 one-day old male Arbor Acres broilers (40.47 ± 0.30 g) were randomly allotted to 4 groups, and each group consisted of 6 replicate pens with 10 broilers per replicate. Broilers fed a basal diet supplementation with COS at 0 (CON group), 200 (COS 200 group), 400 (COS 400 group), and 800 mg/kg (COS 800 group) for 14 d, respectively. Broilers in the COS supplementation groups had no significant effects on growth performance. Compared to the CON group, dietary COS supplementation increased (P < 0.05) the relative weight of duodenum, jejunal lipase activity, duodenal and ileal villus surface area, and lower (P < 0.05) ileal amylase and alkaline phosphatase activity, and crypt depth. The expression level of duodenal glucose transporter 1 (GLUT1), Na + -glucose cotransporter 1 (SGLT1), peptide transporter 1 (PepT1), occludin, zonula occludens-1 (ZO-1), toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), and interleukin-10 (IL-10), jejunal SGLT1, PepT1, occludin, tumor necrosis factor-α (TNF-α), and ileal SGLT1, PepT1, and fatty acid binding protein 1 (FABP1) was upregulated by COS. However, the expression level of duodenal FABP1 and TNF-α, jejunal GLUT1, ZO-1, TLR4, MyD88, nuclear factor kappa-B p65 (NF-κB p65), and IL-1β, and ileal GLUT1, NF-κB p65, and IL-1β was downregulated by COS. Furthermore, dietary COS supplementation increased duodenal catalase (CAT), glutathione peroxidase (GSH-Px), and total superoxide dismutase (T-SOD) activity, jejunal CAT and T-SOD activity, upregulated the expression level of duodenal nuclear factor-erythroid 2-related factor 2 (Nrf2), CAT, glutathione peroxidase 1 (GPX1), and copper and zinc superoxide dismutase (Cu/Zn SOD), jejunal CAT, and ileal Nrf2, CAT, and GPX1. These results suggested that COS could promote intestinal development and functions in broilers aged d 1 to 14, which might be mediated by alleviating intestinal inflammatory response and enhancing antioxidant capacity., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)

The purpose of this study was to evaluate the effects of chitosan oligosaccharides (COS) on acute heat stress (AHS) induced poor meat quality by alleviating oxidative damage through mitogen-activated protein kinase-nuclear factor-erythroid 2-related factor 2-antioxidant responsive element (MAPK-Nrf2-ARE) signaling pathway. A total of 108 thirty-five-day-old Chinese indigenous broilers (Luhua chicken) was used for this 42-d experiment. The broilers were randomly allocated to 3 treatments: control group (CON), AHS group, and AHS with 400 mg/kg COS supplementation (AHS-C) group. Both CON and AHS groups given the basal diet, and the AHS-C group given the basal diet with 400 mg/kg COS supplementation. On d 42, broilers in the AHS and AHS-C groups treated with AHS (increasing temperature from 24 to 34°C in 2-h and held for another 2-h), and the CON group under normal temperature (24°C). AHS exposure elevated (P < 0.05) body temperature (rectal, comb, eyelids, and feet) of broilers, increased (P < 0.05) breast muscle lightness (L*), drip loss, share force, hydrogen peroxide (H 2 O 2 ) scavenging activity, reactive oxygen species (ROS) production, malondialdehyde (MDA) content, and catalase (CAT) activity, however, decreased (P < 0.05) pH 45min , pH 24h , redness (a*), and relative expression of heme oxygenase-1 (HO-1). Compared to the AHS group, dietary COS supplementation increased (P < 0.05) breast muscle pH 45min , pH 24h , and a*, H 2 O 2 scavenging activity, as well as relative expression of HO-1 and glutathione peroxidase (GSH-Px), however, decreased (P < 0.05) drip loss, share force, superoxide anion free radicals (O 2 •- ) scavenging activity, ROS production, and MDA content. It was concluded that AHS impaired meat quality, which may be related to oxidative damage, as evidenced by increasing ROS production, MDA content, and decreasing the relative expression of HO-1. Dietary COS supplementation could effectively elevate the meat quality of broilers exposed to AHS via decreasing ROS production, activating the Nrf2 pathway, and Nrf2-mediated HO-1 and GSH-Px gene expression., (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)

This study was to verify the effects of chitosan oligosaccharides (COS) on intestinal integrity, oxidative status, and inflammatory response in a heat-stressed rat model. A total of 24 male Sprague Dawley rats were randomly divided into 3 treatment: CON, the control group; HS, the heat stress group; HSC, the heat stress group with 200 mg/kg COS. Rats in the HS and HSC group exposed to a cyclical heat stress for 7 consecutive days. The CON and HS group provided basal diet, and the HSC group provided the same diet with 200 mg/kg COS. Compared with the HS group, rats in the HSC group had lower serum diamine oxidase and D-lactate acid level, higher villus height of jejunum and ileum, lower malondialdehyde (MDA) content in duodenum, jejunum, and ileum mucosa, higher glutathione peroxidase (GSH-Px), catalase (CAT) and total antioxidant capacity (T-AOC) activity in duodenum mucosa, higher T-AOC activity in jejunum mucosa, and higher glutathione (GSH) level in ileum mucosa. Compared with the HS group, rats in the HSC group had higher interleukin-10 (IL-10) level, but lower tumor necrosis factor-α (TNF-α) level in duodenum, jejunum, and ileum mucosa. These results indicated that COS may alleviate intestinal damage under heat stress condition, probably by modulating intestinal inflammatory response and oxidative status.

The aim of this study was to evaluate the effects of the dietary supplementation of chitosan oligosaccharides (COS) on intestinal integrity, oxidative status, and the inflammation response with hydrogen peroxide (H 2 O 2 ) challenge. In total, 30 rats were randomly assigned to three groups with 10 replications: CON group, basal diet; AS group, basal diet + 0.1% H 2 O 2 in drinking water; ASC group, basal diet + 200 mg/kg COS + 0.1% H 2 O 2 in drinking water. The results indicated that COS upregulated ( p < 0.05) villus height (VH) of the small intestine, duodenum, and ileum; mucosal glutathione peroxidase activity; jejunum and ileum mucosal total antioxidant capacity; duodenum and ileum mucosal interleukin (IL)-6 level; jejunum mucosal tumor necrosis factor (TNF)-α level; duodenum and ileum mucosal IL-10 level; the mRNA expression level of zonula occludens (ZO)-1 in the jejunum and ileum, claudin in the duodenum, nuclear factor-erythroid 2-like 2 in the jejunum, and heme oxygenase-1 in the duodenum and ileum; and the protein expression of ZO-1 and claudin in jejunum; however, it downregulated ( p < 0.05) serum diamine oxidase activity and D-lactate level; small intestine mucosal malondialdehyde content; duodenum and ileum mucosal IL-6 level; jejunum mucosal TNF-α level; and the mRNA expression of IL-6 in the duodenum and jejunum, and TNF-α in the jejunum and ileum. These results suggested COS could maintain intestinal integrity under oxidative stress by modulating the intestinal oxidative status and release of inflammatory cytokines.

The purpose of this study was to evaluate the effects of chitosan oligosaccharides (COS) on intestinal permeability, morphology, antioxidant status, and inflammatory response in heat-stressed broilers. A total of 108 thirty-five-day-old Chinese yellow-feather broilers (body weight 470.31 ± 13.15 g) were randomly allocated to 3 dietary treatments as follows: CON group, basal diet and raised under normal temperature (24°C); HS group, basal diet and raised under cycle heat stress (34°C from 10:00-18:00 and 24°C for the rest time); HSC group, basal diet with 200 mg/kg COS supplementation and raised under cycle heat stress. Each treatment had 6 replication pens and 6 broilers per pen. Compared with the CON group, heat stress decreased (P < 0.05) the relative weight of duodenum and jejunum; the relative length and villus height (VH) of duodenum, jejunum, and ileum; the ileum VH to crypt depth ratio; duodenum mucosal catalase (CAT) activity; and jejunum mucosal glutathione peroxidase (GSH-Px) and CAT activity, whereas it increased (P < 0.05) serum diamine oxidase (DAO) activity and D-lactate acid (D-LA) content, duodenum and jejunum mucosal malondialdehyde (MDA) and interleukin-1β (IL-1β) content, and ileum mucosal tumor necrosis factor-α content. Compared to the HS group, dietary COS supplementation increased (P < 0.05) the relative length of duodenum, jejunum, and ileum; the VH of jejunum and ileum; and duodenum and jejunum mucosal GSH-Px activity, whereas it decreased (P < 0.05) serum DAO activity and D-LA concentration and duodenum and jejunum mucosal MDA and IL-1β content. These results suggested that dietary COS supplementation had beneficial effects on intestinal morphology by increasing jejunum and ileum VH; permeability by decreasing serum DAO activity and D-LA content; antioxidant capacity by decreasing duodenum and jejunum mucosal MDA content and by increasing duodenum and jejunum GSH-Px activity; and inflammatory response by decreasing duodenum and jejunum mucosal IL-1β content., (Copyright © 2020. Published by Elsevier Inc.)

This study investigated the effects of dietary chitosan oligosaccharides (COS) supplementation on growth performance; corticosterone, growth hormone, and insulin-like growth factor-1 concentration; relative organ weight; liver function; meat quality; muscle glycolytic metabolism; and oxidative status in yellow-feather broilers under heat stress. A total of 108 35-day-old Chinese yellow-feather broilers (BW, 470.31 ± 13.15 g) was randomly allocated to 3 dietary treatments as follow: control group, basal diet and raised under normal temperature (24°C); HS group, basal diet and raised under cycle heat stress (34°C from 10:00 to 18:00 and 24°C for the rest time); and HSC group, basal diet with 200 mg/kg COS supplementation and raised under cycle heat stress. Each treatment had 6 replication pens and 6 broilers per pen. Results indicated that heat stress decreased ADG, ADFI, gain:feed ratio, the relative weight of thymus, bursa of Fabricius, pancreas, proventriculus, gizzard, and liver, growth hormone concentration, pH 24h , muscle glycogen content, muscle superoxide dismutase and glutathione peroxidase activity, as well as increased corticosterone, alanine aminotransferase and aspartate aminotransferase level, cooking loss, muscle lactate and malondialdehyde content. Compared with the HS group, broilers in the HSC group had higher ADG, the relative weight of thymus, bursa of Fabricius, and liver, growth hormone concentration, pH 24h , muscle glycogen content, muscle superoxide dismutase and glutathione peroxidase activity, and lower serum corticosterone, alanine aminotransferase and aspartate aminotransferase level, cooking loss, and muscle lactate and malondialdehyde content. In conclusion, the results suggested that COS could be used as an effective feed additive to maintain growth performance, liver function, meat quality, muscle glycolytic metabolism, and oxidative status of yellow-feather broilers under heat stress. The improved meat quality is possibly through reducing muscle glycolysis metabolism and improving muscle oxidative status by dietary COS supplementation in broilers under heat stress., (Copyright © 2020. Published by Elsevier Inc.)

This study investigated the effects of dietary chitosan oligosaccharide (COS) supplementation on meat quality, antioxidant capacity, and muscle fiber characteristics in the thigh muscle of broilers. The results showed that dietary COS supplementation decreased shear force and increased crude protein content and nutritional value in the thigh muscle, while decreasing the content of C16:0, C18:0, and total saturated fatty acids. Dietary COS supplementation increased free radical scavenging activity, antioxidant enzyme activity, and antioxidant enzyme-related gene expression. Additionally, COS promoted MyHCI while decreasing MyHCIIb mRNA expression levels. The myofiber transformation was associated with upregulated gene expression of CaN, NFATc1, MyoD, and SIRT1. Together, the results of this study demonstrate that dietary COS supplementation improves meat quality, nutritional value, antioxidant capacity, and myofiber transformation to more oxidative muscle fibers in the thigh muscle of broilers when its supplemental level is 400 mg/kg. [ABSTRACT FROM AUTHOR]

Heat stress has been reported to induce hepatic oxidative stress and alter lipid metabolism and fat deposition in broilers. Chitosan oligosaccharides (COSs), a natural oligosaccharide, has anti-oxidant, anti-inflammatory, and lipid-lowering effects. This study is conducted to evaluate dietary COS supplementation on hepatic anti-oxidant capacity, inflammatory response, and lipid metabolism in heat-stressed broilers. The results indicate that heat-stress-induced poor (p < 0.05) growth performance and higher (p < 0.05) abdominal adiposity are alleviated by COS supplementation. Heat stress increases (p < 0.05) serum AST and ATL activity, serum and liver MDA, TG, TC, and LDL-C levels, and the expression of hepatic IL-1β, IL-6, SREBP-1c, ACC, and FAS, while it decreases (p < 0.05) serum SOD and CAT activity, liver GSH-Px and SOD activity, and the expression of hepatic Nrf2, GPX1, IL-10, MTTP, PPARα, and CPT1. Nevertheless, COS supplementation decreases (p < 0.05) serum AST and ATL activity, serum and liver MDA, TG, TC, and LDL-C levels, and the expression of hepatic IL-1β, IL-6, SREBP-1c, ACC, and FAS, while it increases (p < 0.05) serum SOD and CAT activity, liver GSH-Px activity, and the expression of hepatic Nrf2, CAT, IL-10, LPL, MTTP, PPARα, and CPT1. In conclusion, COS could alleviate heat-stress-induced lipid metabolism disorders by enhancing hepatic anti-oxidant and anti-inflammatory capacity. [ABSTRACT FROM AUTHOR]

Context: Pre-slaughter transport, exposed broilers to various stressors, which resulted in detrimental effects on animal welfare, live bodyweight loss, mortality and meat quality. There was growing interest to explore effective ways to reduce the stress response and improve meat quality of transported broilers by using dietary feed additives. Aims: The purpose of the present study was to investigate the effect of chitosan oligosaccharides (COS) on meat quality, muscle energy metabolism and anti-oxidant status of broilers having 3-h transport stress. Methods: In total, of 144 35-day-old broilers were randomly allocated to two dietary treatments, including a basal diet (96 broilers), or basal diet supplemented with COS at 200 mg/kg (48 broilers). There were eight broilers per cage, and 12 replication cages in the basal diet group and six replication cages in the basal diet with 200 mg/kg COS group. At the end of the experiment, after a 12-h fast, broilers in the basal diet group were randomly divided into two groups, with six replication cages in each group; then, the broilers were transported as per the following protocols: broilers in the basal diet group (CON group) were without transport stress; broilers in the basal diet group with 3 h of transport stress (TS group), and broilers in the basal diet with 200 mg/kg COS supplementation and 3 h of transport stress (TSC group). The serum corticosterone concentration, serum, liver and muscle anti-oxidant status, as well as meat quality and muscle energy metabolism were analysed. Key results: Compared with the CON group, TS group showed increased bodyweight loss, serum corticosterone concentration, breakdown of muscle glycogen, increased muscle lactate dehydrogenase activity, as well as some changes of body anti-oxidant status (higher serum, liver and muscle MDA concentrations, lower serum SOD and GSH-Px, liver SOD and CAT, and lower muscle SOD, GSH-Px, and CAT activity), accompanied with lower pH45min and pH24h. Nevertheless, compared with the TS group, dietary COS supplementation reduced bodyweight loss, decreased muscle MDA concentration, increased muscle SOD and CAT activity, and was accompanied with improving pH24h. Conclusions: The results suggested that the positive effects of dietary COS supplementation in maintaining meat quality were mainly due to the improved muscle anti-oxidant status. Implications: Dietary supplementation with 200 mg/kg COS could serve as a beneficial and effective way to alleviate transport-impaired meat quality of broilers. Pre-slaughter transport, an unavoidable common practice, results in detrimental effects on meat quality of broilers. Three-hour transport decreased muscle glycogen content, and SOD, GSH-Px and CAT activity, increased muscle lactate dehydrogenase activity and MDA concentration, and was accompanied with lower pH45min and pH24h. Dietary COS supplementation decreased muscle MDA concentration, increased muscle SOD and CAT activity, and was accompanied with an improved pH24h. In conclusion, dietary COS supplementation could maintain meat quality by improving muscle anti-oxidant capacity. [ABSTRACT FROM AUTHOR]

Simple Summary: Oxidative stress adversely affects animal health and performance. Feed additives with antioxidant abilities supplementation can alleviate oxidative stress. The aim of this study was to evaluate the hypothesis that dietary supplementation with COS alleviates the damage caused by oxidative stress in Sprague Dawley rats challenged with hydrogen peroxide (H2O2). The results shown that COS exhibited better radical scavenging capacity of 1, 1-diphenyl-2-picrylhydrazyl (DPPH), superoxide anion (O2−), H2O2, and ferric ion reducing antioxidant power (FRAP) than butylated hydroxy anisole (BHA), increasing activity of SOD, CAT, GSH-Px, GSH, and T-AOC, as well as decreasing MDA level in serum, liver, spleen, and kidney. Our results indicated that COS can protect Sprague Dawley rats from H2O2 challenge by reducing lipid peroxidation and restoring antioxidant capacity. Oxidative stress is induced by excessive oxidative radicals, which directly react with biomolecules, and damage lipids, proteins and DNA, leading to cell or organ injury. Supplementation of antioxidants to animals can be an effective way to modulate the antioxidant system. Chitosan oligosaccharides (COS) are the degraded products of chitosan or chitin, which has strong antioxidant, anti-inflammatory, and immune-enhancing competency. Therefore, the current study was conducted to evaluate the hypothesis that dietary supplementation with COS alleviates the damage caused by oxidative stress in Sprague Dawley rats challenged with hydrogen peroxide (H2O2). The rats were randomly divided into three groups: CON, control group, in which rats were fed a basal diet with normal drinking water; AS, H2O2 group, in which rats were fed the basal diet and 0.1% H2O2 in the drinking water; ASC, AS + COS group, in which rats were fed the basal diet with 200 mg/kg COS, and with 0.1% H2O2 in the drinking water. In vitro, COS exhibited better radical scavenging capacity of 1, 1-diphenyl-2-picrylhydrazyl (DPPH), superoxide anion (O2−), H2O2, and ferric ion reducing antioxidant power (FRAP) than butylated hydroxy anisole (BHA). In vivo, dietary supplementation with COS alleviated the H2O2-induced oxidative damage, evidenced by comparatively increasing activity of SOD, CAT, GSH-Px, GSH, and T-AOC, and comparatively decreasing level of MDA in serum, liver, spleen, and kidney. COS also comparatively alleviated the H2O2-induced inflammation. In conclusion, COS supplementation reduced lipid peroxidation and restored antioxidant capacity in Sprague Dawley rats, which were challenged with H2O2. [ABSTRACT FROM AUTHOR]

Simple Summary: Heat stress has negative effects on animal health and performance, and chitosan oligosaccharides (COS) exhibits antioxidant and anti-inflammatory properties. The aim of this study was to evaluate the effects of COS alleviation of oxidative stress and inflammatory response in heat-stressed rats. The results indicated heat stress decreased (p < 0.05) growth performance; the relative weight of spleen and kidney; and the level of antioxidant enzymes and IL-10 in liver, spleen, and kidney, while it increased (p < 0.05) the MDA and inflammatory cytokines concentration. Dietary COS supplementation enhanced (p < 0.05) ADG, the relative weight of spleen and kidney, and the level of antioxidant enzymes and IL-10 in liver, spleen, and kidney. Collectively, COS was beneficial to heat-stressed rats by alleviating oxidative damage and inflammatory response. Chitosan oligosaccharides (COS) exhibits antioxidant and anti-inflammatory properties. The aim of this study was to evaluate the effects of COS on antioxidant system and inflammatory response in heat-stressed rats. A total of 30 male rats were randomly divided to three groups and reared at either 24 °C or 35 °C for 4 h/d for this 7-day experiment: CON, control group with basal diet; HS, heat stress group with basal diet; HSC, heat stress with 200mg/kg COS supplementation. Compared with the CON group, HS significantly decreased (p < 0.05) average daily gain (ADG); average daily feed intake (ADFI); the relative weight of spleen and kidney; the level of liver CAT, GSH-Px, T-AOC, and IL-10; spleen SOD, GSH-Px, GSH, and IL-10; and kidney SOD, GSH-Px, T-AOC, and IL-10, while significantly increased the MDA concentration in liver, spleen, and kidney; the liver IL-1β concentration; and spleen and kidney IL-6 and TNF-α concentration. In addition, dietary COS supplementation significantly improved (p < 0.05) ADG; the relative weight of spleen and kidney; the level of liver GSH-Px, spleen GSH-Px, GSH, and IL-10; and kidney GSH-Px, while significantly decreased (p < 0.05) liver IL-1β concentration under heat stress condition. Collectively, COS was beneficial to heat-stressed rats by alleviating oxidative damage and inflammatory response. [ABSTRACT FROM AUTHOR]