1. Inflammation modifies the pattern and the function of Toll-like receptors expressed by human mesenchymal stromal cells.
- Author
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Raicevic G, Rouas R, Najar M, Stordeur P, Boufker HI, Bron D, Martiat P, Goldman M, Nevessignsky MT, and Lagneaux L
- Subjects
- Cells, Cultured, Cytokines genetics, Cytokines immunology, Gene Expression Regulation drug effects, Gene Expression Regulation immunology, Humans, Immune Tolerance drug effects, Inflammation, Lipopolysaccharides pharmacology, Mesenchymal Stem Cells drug effects, Mesenchymal Stem Cells immunology, Mesenchymal Stem Cells pathology, Poly I-C pharmacology, Stromal Cells drug effects, Stromal Cells immunology, Stromal Cells pathology, Toll-Like Receptors genetics, Toll-Like Receptors immunology, Cytokines biosynthesis, Mesenchymal Stem Cells metabolism, Stromal Cells metabolism, Toll-Like Receptors biosynthesis
- Abstract
Mesenchymal stromal cells (MSC) are involved in tissue repair and in the regulation of immune responses. MSC express Toll-like receptors (TLR) known to link innate and adaptive immunity. We hypothesized that TLR signaling could influence human MSC (hMSC) function. Here, we show that hMSC express TLR1, TLR2, TLR3, TLR4, TLR5, and TLR6 but not TLR7, TLR8, TLR9, and TLR10. In inflammatory conditions mimicked by culturing hMSC in an inflammatory environment, TLR2, TLR3, and TLR4 are upregulated, whereas TLR6 is downregulated. Interleukin (IL)-1 beta, IL-6, IL-12p35 and transforming growth factor-beta mRNAs are constitutively expressed by hMSC. Inflammation leads to an increase in IL-1 beta, IL-6, IL-12p35, and transforming growth factor-beta transcription and is characterized by IL-23p19 and IL-27p28 transcription. In this setting, poly(I:C) further augments IL-6, IL-12p35, IL-23p19, and IL-27p28 transcription, whereas lipopolysaccharide (LPS) increases IL-23p19 and IL-27p28 transcription. By upregulating TLR3 and TLR4 transcription, inflammation increases the hMSC responsiveness to LPS and poly(I:C), leading to a proinflammatory shift in their cytokine profile. The hMSC osteogenic potential does not change after TLR triggering but stimulation with LPS and poly(I:C) results in a decrease in their immunosuppressive capabilities. In conclusion, TLR activation in hMSC may affect their function and could modify their in vivo fate, especially in an inflammatory context., ((c) 2010 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.)
- Published
- 2010
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