1. LC-MS Identification, Isolation, and Structural Elucidation of Anti-HIV Tigliane Diterpenoids from Wikstroemia lamatsoensis .
- Author
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Zhang M, Otsuki K, Kikuchi T, Bai ZS, Zhou D, Huang L, Chen CH, Morris-Natschke SL, Lee KH, Li N, Koike K, and Li W
- Subjects
- Anti-HIV Agents pharmacology, Cell Line, China, Diterpenes pharmacology, HIV-1 drug effects, Humans, Molecular Structure, Phorbols pharmacology, Phytochemicals chemistry, Phytochemicals pharmacology, Anti-HIV Agents chemistry, Diterpenes chemistry, Phorbols chemistry, Wikstroemia chemistry
- Abstract
Structurally diverse tigliane diterpenoids have drawn significant research interest for drug discovery over many decades. Using LC-MS-guided fractionation and separation, the first phytochemical investigation on Wikstroemia lamatsoensis led to the isolation of eight tiglianes ( 1 - 8 ), including two new compounds, wikstrocin D ( 1 ) and wikstrocin E ( 2 ). The new structures were elucidated based on extensive physicochemical and spectroscopic analyses. The characteristic ESIMS/MS fragmentations of tiglianes 1 - 8 were also summarized. Among the isolated tiglianes, three compounds ( 8 , 5 , and 7 ) showed the most potent anti-HIV activity, with IC
50 values of 0.18, 3.8, and 12.8 nM, respectively.- Published
- 2021
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