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The short shelf life of Portunus trituberculatus is an important factor limiting the progress of this industry. Electron beam irradiation (EBI) can effectively kill the microorganisms, but the effect of EBI on crab muscle tissue remains unknown. In this study, a proteomics method based on labeling with the tandem-mass tag (TMT) was used to study the protein changes occurring in crabs under different doses of EBI at 0, 2, 4, 6, 8, and 10 kGy. A total of 6123 proteins were identified, of which 1490 were significantly differentially expressed proteins (DEPs), and 91 were common DEPs. An in-depth data analysis showed that the DEPs were involved in various cellular processes, particularly metabolic processes. We combined gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses, and our results focused on oxidative phosphorylation, tight junction, and oxytocin signaling pathways. Key proteins responsible for the effects of EBI on crabs were also found. [ABSTRACT FROM AUTHOR]

Postmortem metabolism in shrimp is an important factor affecting muscle quality during its preservation. Electron beam irradiation (EBI) can effectively kill microorganisms and inactivate enzymes. In this study, the tandem mass tag (TMT) method was used to study the changes in the muscle metabolism of Solenocera melantho postmortem before and after EBI (6 kGy). The TMT method identified a total of 656 proteins, including 120 differentially expressed proteins (DEPs) (56 upregulated, 64 downregulated). The analysis of these results showed that EBI can inactivate most enzymes and slow down their corresponding metabolic processes. These results also showed that irradiation can denature some proteins. The study revealed the effects of EBI on the muscle quality of S. melantho at the molecular level. [ABSTRACT FROM AUTHOR]

Electron beam irradiation (EBI) can effectively kill microorganisms that cause food decay and thus, prolong the shelf life of food. However, different doses of EBI on the impact of Portunus trituberculatus microbial community structure remain to be seen. This study adopted high-throughput sequencing technologies to compare the effect of different doses of EBI on the microbial community structure of the swimming crab and found that the crab microbial community structure was changed significantly upon treatment with different doses of EBI. We combined alpha and beta diversity analysis, relative abundance analysis of species, and linear discriminant analysis effect size analysis, and our results focused on Psychrilyobacter , Clostridium , Vagococcus , Psychrobacter , and Carnobacterium. In addition, the richness and diversity of microorganisms initially decreased and then increased with the increasing of radiation dose. The results showed that the minimum dose of EBI was 6 kGy. This study provides a theoretical basis for the preservation of crabs. [Display omitted] • The effects of EBI on the microflora of Portunus trituberculatus were studied by 16S rRNA. • Bacteria richness and diversity decreased firstly and then increased with increasing EBI dose. • EBI may inhibit the growth of Pseudomonas bacteria to prevent the occurrence of BPS. • Psychrobacter dominated the P. trituberculatus after 6 kGy dose of EBI. [ABSTRACT FROM AUTHOR]

Litopenaeus vannamei (L. vannamei) has high moisture, protein, and strong enzyme activity, which makes the shrimp susceptible to spoilage and deterioration after death. The study investigated the effects of different refrigerated conditions on the sensory quality, physicochemical indicators, and bacterial communities of L. vannamei , including crushed ice (CI, −0.5 °C), freshwater slurry ice (FI, −1 °C), seawater slurry ice (SI, −1.7 °C), and electron beam irradiation combined with seawater slurry ice (ESI, −1.7 °C), of which CI was the control group. All samples were stored in a freezer at −2 °C to keep the experimental conditions consistent. The results of sensory and physicochemical indexes showed that, compared with the other groups, the ESI group effectively inhibited the activities of polyphenol oxidase, significantly slowed down the growth of pH, TVB-N, and TVC values, and maintained the good organoleptic quality of the shrimp. High-throughput sequencing analyzes showed that the relative abundance of Vibrio and Photobacterium decreased significantly after irradiation, while the uniformity of the microorganisms increased. After 16 days of refrigeration, the relative abundance of Pseudoalteromonas , Photobacterium , and Alistipes in the ESI group was lower compared to the CI group, and the colony structure appeared to be more uniform. Therefore, the combination of electron beam irradiation and seawater slurry ice technology is promising for extending the shelf life of shrimp. • EBI combined with SI treatment effectively extended the shelf life of shrimp. • ESI treatment slowed down the growth of pH, TVB-N, TVC values of the shrimp. • ESI treatment inhibited enzyme and microbial activity. • The abundance and diversity of spoilage bacteria in the ESI-16 group were lower. [ABSTRACT FROM AUTHOR]

• Electron beam irradiation (EBI) was used to treat the shrimp. • Metabolomics was used to study metabolites changes of shrimp during refrigeration. • Two potential biomarkers were discovered through metabolomics. • Quality change of shrimp during refrigeration was analyzed by metabolic pathways. Shrimp meat is an extremely perishable product; however, refrigeration can slow down spoilage. In this study, we used electron beam irradiation (EBI) to pre-treat shrimp meat and analyzed the metabolites of the treated shrimp meat during refrigerated storage using metabonomic analysis methods. In total, 4865 metabolites were identified, of which, 103 differential metabolites had KEGG (Kyoto Encyclopedia of Genes and Genomes) IDs. Further, two potential biomarkers were obtained. Based on the results, l -lysine was downregulated, while 2′-deoxyguanosine 5′-monophosphate and dihydroxyacetone phosphate acyl ester were upregulated during the refrigerated storage. The metabolic activity began to weaken gradually after 9 days. However, the different metabolites related to EBI were not identified herein. Nonetheless, the study findings revealed the metabolic changes in Solenocera melantho at the molecular level during refrigerated storage after EBI. [ABSTRACT FROM AUTHOR]

Solenocera melantho was irradiated at different doses, and the physiological and biochemical indicators, total viable count, and 16S rRNA were used to study the effect of radiation treatment on the quality of S. melantho. Results showed that irradiating at a dose of 2 kGy killed most microorganisms. The higher the irradiation dose used, the stronger the bactericidal effect observed. The bacterial community structure of the irradiate samples were significantly different from that of the unirradiated samples. Irradiation had no effect on the pH value, although as the irradiation dose increased, polyphenol oxidase activity was effectively inhibited, L* and a* values of the shrimp increased, and springiness of the shrimp muscle was enhanced. Based on these results, an irradiation dose of 6 kGy was found to be the most suitable for the treatment of S. melantho , which provides a theoretical basis for production and processing. • Electron beam irradiation (EBI) was used to treat Solenocera melantho. • The optimal EBI dose for treating Solenocera melantho was determined. • 16S rRNA was used to analyze microbial diversity at the end of the preservation. • Shewanella and Pseudomonas were killed by EBI. [ABSTRACT FROM AUTHOR]