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Due to their high porosity, large specific surface area, and structural similarity with the extracellular matrix (ECM), electrospun nanofiber membranes are often endowed with the antibacterial properties for biomedical applications. The purpose of this study was to synthesize nano-structured Sc2O3-MgO by doping Sc3+, calcining at 600 °C, and then loading it onto the PCL/PVP substrates with electrospinning technology with the aim of developing new efficient antibacterial nanofiber membranes for tissue engineering. A scanning electron microscope (SEM) and energy dispersive X-ray spectrometer (EDS) were used to study the morphology of all formulations and analyze the types and contents of the elements, and an X-ray diffraction (XRD), thermogravimetric analysis (TGA), and Fourier transform attenuated total reflection infrared spectroscopy (ATR-FTIR) were used for further analysis. The experimental results showed that the PCL/PVP (SMCV-2.0) nanofibers loaded with 2.0 wt% Sc2O3-MgO were smooth and homogeneous with an average diameter of 252.6 nm; the antibacterial test indicated that a low load concentration of 2.0 wt% Sc2O3-MgO in PCL/PVP (SMCV-2.0) showed a 100% antibacterial rate against Escherichia coli (E. coli). [ABSTRACT FROM AUTHOR]

Antibacterial and active packaging materials have gained significant research attention in response to the growing interest in food packaging. In this investigation, we developed hydrogel packaging materials with antibacterial and antioxidant properties by incorporating chitooligosaccharide (COS) and fish skin gelatin (FSG) nanofiber membranes, which readily absorbed water and exhibited swelling characteristics. The nanofiber membranes were fabricated by electrospinning technology, embedding COS within FSG, and subsequently crosslinked through the Maillard reaction facilitated by the addition of glucose. The behavior of conductivity, viscosity, and surface tension in the spinning solutions was analyzed to understand their variation patterns. Scanning electron microscopy (SEM) results revealed that the crosslinked COS/FSG nanofiber membranes possessed a uniform yet disordered fiber structure, with the diameter of the nanofibers increasing as the COS content increased. Remarkably, when the COS content reached 25 %, the COS/FSG nanofiber membranes (CF-C-25) exhibited a suitable fiber diameter of 437.16 ± 63.20 nm. Furthermore, the thermal crosslinking process involving glucose supplementation enhanced the hydrophobicity of CF-C-25. Upon hydration, the CF-H-25 hydrogel displayed a distinctive porous structure, exhibiting a remarkable swelling rate of 954 %. Notably, the inclusion of COS significantly augmented the antibacterial and antioxidant properties of the hydrogel-based nanofiber membranes. CF-H-25 demonstrated an impressive growth inhibition of 90.56 ± 5.91 % against E. coli , coupled with excellent antioxidant capabilities. In continuation, we performed a comprehensive analysis of the total colony count, pH, TVB-N, and TBA of crucian carp. The CF-H-25 hydrogel proved highly effective in extending the shelf life of crucian carp by 2–4 days, suggesting its potential application as an edible membrane for aquatic product packaging. [Display omitted] • Nanofibers composed of COS/FSG were synthetized via the electrospinning technique. • Hydrogel was prepared by water absorption swelling of nanofibers after Maillard reaction. • COS/FSG nanofiber-based hydrogel was fabricated for food packaging. • COS/FSG nanofiber-based hydrogel have significant antibacterial and antioxidant properties. [ABSTRACT FROM AUTHOR]

This article utilizes two ingestible substrates, pullulan and pectin, to fabricate nanofiber membranes by means of electrospinning technology, and assessed their digestion characteristics in simulated gastrointestinal fluids. The physicochemical properties of pullulan/pectin spinning solution and the effects of electrospinning process parameters on the diameter and morphology of pullulan/pectin nanofibers were investigated. Scanning electron microscope indicates that the best electrospinning process parameters were obtained as spinning voltage: 20 kV, needle-collector distance: 15 cm, propulsion speed: 0.6 mL/h and needle inner diameter: 0.41 mm. Moreover, at a pullulan/pectin volume ratio of 8:2, the nanofiber morphology exhibited optimal characteristics, featuring an average fiber diameter of 369.45 ± 42.41 nm. Fourier transform infrared spectroscopy, X-ray diffraction, and thermal analysis indicates the formation of strong hydrogen bonds between pullulan and pectin. The mechanical performance tests demonstrated that, with a volume ratio of 8:2 for pullulan and pectin, the nanofiber exhibited a tensile strength of 1196.86 ± 51.87 cN and an elongation at break of 80.26 ± 3.6%, indicating favorable capacity for elongation and deformation. Furthermore, the in vitro degradability test indicates that pullulan/pectin nanofibers with a volume ratio of 8:2 were decomposed by specific enzymes in intestinal fluid. Therefore, ingestible pullulan/pectin nanofiber substrates have shown certain development potential in the field of targeted drug or bioactive delivery. • Smooth and uniform PUL/PEC fiber substrates were prepared by electrospinning. • Nanofibers were designed using blend of pullulan and pectin with a mass ratio of 8: 2. • Colon-specific decomposition property was evaluated by an in vitro degradability test. • PUL/PEC nanofibers showed potential as colon delivery systems. [ABSTRACT FROM AUTHOR]

In order to fabricate antibacterial nanofiber membranes for tissue engineering field, the oxidative damage of magnesium hydroxide nanoparticles (Mg(OH) 2 NPs) against the model system of Escherichia coli (E. coli) was researched by measuring the oxidative stress response, oxidative stress products and glucose metabolism. The toxicity of Mg(OH) 2 NPs to a normal biological system was evaluated in vivo through the acute oral toxicity, blood physiological and serum biochemical markers and acute skin irritation, indicating that the Mg(OH) 2 NPs exhibited great biosafety. The Mg(OH) 2 NPs with antibacterial property and biosafety were loaded on PCL/PVP nanofiber substrates through electrospinning technology to fabricate the Mg(OH) 2 /PCL/PVP nanofiber membranes. The results of characterization and antibacterial test showed that the Mg(OH) 2 /PCL/PVP nanofiber membranes with 8.0 wt% Mg(OH) 2 NPs present excellent antibacterial rate of 100% against E. coli that the Mg(OH) 2 NPs can be physically and evenly loaded on nanofiber substrates. • Oxidative damage of Mg(OH) 2 NPs against E. coli was evaluated. • Biosafety of Mg(OH) 2 NPs was evaluated in vivo. • Mg(OH) 2 /PCL/PVP nanofiber membranes were fabricated for the first time. • Mg(OH) 2 NPs were loaded on nanofiber substrates by electrospinning method. [ABSTRACT FROM AUTHOR]