1. A novel ferritin L (FerL) in hybrid crucian carp could participate in host defense against Aeromonas hydrophila infection and diminish inflammatory signals.
- Author
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Xiong NX, Ou J, Li SY, Zhao JH, Huang JF, Li KX, Luo SW, Liu SJ, Wen M, Wu C, Wang S, Luo KK, Hu FZ, and Liu QF
- Subjects
- Aeromonas hydrophila, Animals, Immunity, Innate genetics, Iron, Carps genetics, Carps immunology, Ferritins genetics, Fish Diseases microbiology, Fish Proteins genetics, Fish Proteins metabolism, Gram-Negative Bacterial Infections veterinary
- Abstract
FerL, a multifunctional iron-storage polypeptide, not only exhibited a regulatory role in iron metabolism, but also participated in the regulation of fish immunity. In this study, ORF sequence of WR-FerL was 522 bp, encoding 173 amino acid residues. Tissue-specific analysis revealed that the highest expression of WR-FerL was detected in spleen. A. hydrophila challenge and LPS stimulation could sharply enhance WR-FerL mRNA expression in tissues and fish cells, respectively. Purified WR-FerL fusion peptide exhibited in vitro binding activity to A. hydrophila and endotoxin, limited bacterial dissemination to tissues as well as attenuated A. hydrophila-induced production of pro-inflammatory cytokines. Moreover, WR-FerL overexpression could abrogate NF-κB and TNFα promoter activity in fish cells. These results indicated that WR-FerL could play an important role in host defense against A. hydrophila infection., (Copyright © 2021 Elsevier Ltd. All rights reserved.)
- Published
- 2022
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