1. High-Dimensional Immunophenotyping with 37-Color Panel Using Full-Spectrum Cytometry.
- Author
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Fernandez MA, Alzayat H, Jaimes MC, Kharraz Y, Requena G, and Mendez P
- Subjects
- Color, Humans, Immune System, COVID-19 blood, Flow Cytometry, Immunophenotyping methods
- Abstract
A comprehensive study of the cellular components of the immune system demands both deep and broad immunophenotyping of numerous cell subsets in an effective and practical way. Novel full-spectrum technology reveals the complete emission spectrum of each dye maximizing the amount of information that can be obtained on a single sample regarding conventional flow cytometry and provide an expanded knowledge of biological processes. In this chapter, we describe a 37-color protocol that allows to identify more than 45 different cell populations on whole blood samples of SARS-CoV-2-infected patients., (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
- Published
- 2022
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