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Due to the similar structure and reactivity of cysteine (Cys), homocysteine (Hcy) and glutathione (GSH), the simultaneous discrimination of Cys over Hcy and GSH by a single fluorescent sensor is still a great challenge. In this work, a benzothiazole-pyimidine-based boron difluoride complex ( BPB) was developed as a new fluorescent sensor for Cys. The sensor exhibits a highly selective 'turn-on' response to cysteine over Hcy, GSH and other amino acids in aqueous solution at physiological pH. The observed pseudo-first-order rate constant for the reaction of BPB with Cys was calculated to be about 0.062 min−1. The detection limit of this sensor for Cys was determined to be 332 n m, and bioimaging of exogenous Cys by this sensor was successfully applied in living cells, thus indicating that this sensor holds great potential for biological applications. [ABSTRACT FROM AUTHOR]

Hydrogen polysulfides (H 2 S n , n > 1) have attracted increasing attention in biological systems due to its redox signaling effect. To illustrate the process of the physiological and pathological roles played by H 2 S n , accurate detection is highly desired. In this work, we report a BODIPY-based fluorescent probe (BDP-PHS) for ratiometric H 2 S n sensing. BDP-PHS shows higher sensitivity and selectivity ratiometric response toward H 2 S n than various biological related species. Moreover, BDP-PHS has been successfully applied in imaging of H 2 S n in living cells. A new fluorescent probe, BDP-PHS , which exhibits a specific ratiometric response toward H 2 S n than various biological related species in both aqueous media and living cells was developed. Unlabelled Image • A new ratiometric fluorogenic probe (BDP-PHS) for detecting polysulfides was developed. • BDP-PHS shows specific polysulfides-induced emission shift from 574 to 593 nm. • BDP-PHS has been utilized to realize the in vivo ratiometric polysulfides imaging in living cells. [ABSTRACT FROM AUTHOR]