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An "AND" logic gate-based NIR fluorescent probe Si-NH2-Glu was developed based on novel meso-amine Si-Rhodamine, which combined γ-glutamyl transpeptidase and pH dual-responsive sites. The features of Si-NH2-Glu enable it to be applied in orthotopic tumor imaging and fluorescence-guided surgery. [ABSTRACT FROM AUTHOR]

The abnormality of the plasma membrane (PM) is an important biomarker for cell status and many diseases. Hence, visualizing the PM, especially in complex systems, is an emerging field in the life sciences, especially in low‐resource settings. Herein, we developed a water‐soluble PM‐specific probe utilizing electrostatic and hydrophobic interaction strategies with aggregation‐induced emission as the signal output. The probe could image the PM with many advanced features (wash‐free, ultrafast staining process, excellent PM specificity, and good biocompatibility), which were demonstrated by the PM imaging of neurons. The probe allowed for the first time the imaging of erythrocytes in the complex brain environment through a fluorescence‐based method. Moreover, the PM of the epidermal and partial view of the eyeball structure of live zebrafish are also revealed. [ABSTRACT FROM AUTHOR]

A novel reaction-based fluorescent probe (ATC-Hg) was designed and synthesized via the “covalent assembly” principle, which exhibited excellent selectivity and high sensitivity for Hg2+ and CH3Hg+. Moreover, it is the first Hg(ii) sensitive fluorescent probe that has been successfully applied in imaging in both cells and Escherichia coli. [ABSTRACT FROM AUTHOR]

A ratiometric fluorescent probe for SO2 derivatives based on the conjugate of carbazole and indolium was presented, which could selectively respond to HSO3− over other thiol compounds. More importantly, CZ-Id is a novel mitochondria-targeted ratiometric fluorescent probe to image exogenous SO2 derivatives. [ABSTRACT FROM AUTHOR]

A ratiometric fluorescent probe (RMClO-2) for ClO− based on the conjugate of coumarin–rhodamine was presented, which could sense ClO− with fast response (within 5 s), high sensitivity and excellent selectivity. More importantly, RMClO-2 is the first mitochondria-targeted ratiometric fluorescent probe to image exogenous and endogenous ClO−. [ABSTRACT FROM AUTHOR]

A novel chromenylium-based fluorescent probe was exploited for sulphur dioxide (SO2) detecting. The probe displayed a remarkable fluorescence turn-on response towards SO2 based on the nucleophilic addition reaction to the carbon-carbon double bond with 105 nm Stock shift. The probe was successfully applied for the quantification of SO2.The linear detection range was from 0–160 μM with the detection limit as low as 99.27 nM. It also exhibited high selectivity for SO2 than other reactive species and amino acids. Furthermore, cell staining experiments indicated that the probe was cell membrane permeable and could be used for high-performance imaging of SO2 in living cells. The superior properties of the probe made it highly promising for use in chemical and biological applications. [ABSTRACT FROM AUTHOR]

[Display omitted] • The probes can rapidly assess and visualize β -galactosidase during fruit softening. • The probes can be used to evaluate fruit storage methods. • TNap-βGal can distinguishing the senile cells from the young ones within 1 min. • TNap-βGal can visualize the increased β -galactosidase in senile tissues. Senescence is happening in every corner of the living organisms. β -galactosidase (β -gal) is one of the most important biomarkers during senescence in both plant and mammalian cells. Most β -gal fluorescent probes were focused on bio-imaging, only a few probes were developed for the detection of β -gal in fruit, and the probes that could detect β -gal in both fruits and living cells were even less. Here, two β -gal probes (TNap-βGal and TBNap-βGal) were synthesized, which can not only image the increase of β -gal during both fruits softening and cellular senescence, but also prove that bananas are not suitable for storage in refrigerator and the subsequent accumulation of β -gal still in lysosome of mammalian cells. In addition, TNap-βGal was successfully applied to two-photon imaging of endogenous β -gal in both hDPMSCs and tissues of human dental pulp for the first time. [ABSTRACT FROM AUTHOR]

More evidence supports that endoplasmic reticulums stress (ER stress) has close relationship with many disease, including Alzheimer's disease, and such diseases are often closely related to senescence. Both ER stress and senescence involve disorder of redox regulation and may further cause ClO- accumulation. It is of great importance to develop a tool to visualize ralated organelles to investigate the role of ClO- during ER stress or senescence. In this work, a novel dual-targetable ClO- probe RT-ER was designed, and the probe could image endoplasmic reticulums and lysosome by sequence under stimulation of ClO-. RT-ER worked in a ratiometric manner and was extremely sensitive to ClO-(~ 484 folds enchancement within 10 s after the stimulation of ClO-). RT-ER showed great biocompatibility and was further applied in living cells imaging. We demonstrated that RT-ER enables both exogenous and endogenous ClO- imaging. Compared with the commercial dye Lysosome-Tracker Deep Red, RT-ER showed better photobleaching resistance. We successfully used RT-ER to track the interaction between endoplasmic reticulum and lysosome after ClO- accumulation. More importantly, with the help of RT-ER, we further revealed the level of ClO- change during ER stress and senescence, which will facilitate the functional study of ClO- −disease contacts. [Display omitted] • The probe could image ERs and lysososme in two channels by sequence with stimulation of ClO-. • The intereactions between lysosome and ER can be long-term tracked by the probe when ClO- continue to accumulate in cells. • The fluctuation of ClO- during ERs stress and senescence can be monitored by the probe. [ABSTRACT FROM AUTHOR]

• A novel near-infrared nitrite probe based on "covalent assembly" principle; • A efficient and zero background analytical method for quantitative nitrite detection; • Detection of nitrite in river water, food samples, and Escherichia coli. Different chemical states of nitrogen are present in many freshwater and marine ecosystems, and nitrite ions are one of the most toxic water-soluble nitrogen species. Developing an effective and convenient sensing method to constantly detect the concentration of nitrite has become a wide concern. Here, a novel near-infrared fluorescent probe (AAC) was designed and synthesized via the "covalent assembly" principle, showing excellent selectivity and high sensitivity for nitrite. A new nitrite-quantitative method was established with the help of AAC , and the detection limit of nitrite using the new method was as low as 6.7 nM. AAC was successfully applied for the quantitative detection of nitrite in real-world environmental and food samples (including river water and Chinese sauerkraut), and the detection results were essentially identical to the results obtained from the traditional Griess assay. Moreover, AAC was successfully applied for tracking nitrite in Escherichia coli by fluorescence imaging. Since nitrite can have devastating effects, the method established with AAC allowed us to "see" effectively about the water quality, food quality, etc. [ABSTRACT FROM AUTHOR]

• A compound with an AIEgen was firstly synthesized as a G-quadruplex probe. • A efficient and zero background analytical method was successfully developed for the detection of G-quadruplex DNA. • With the help of the probe, DNA with G-quadruplex structures in mitochondrion can be easily, quickly and accurately tracked without further washing operations. Inspired by the mechanism of aggregation induced emission, two derivatives of thiazole orange (TPE-mTO and Ph-TO) were rationally designed and prepared in this work. Their selectivity and sensitivity towards G-quadruplex were studied by fluorescence titration, gel analysis, and circular dichroism (CD) experiments. TPE-mTO could selectively lights-up G-quadruplex DNA structures with no conformational transition, while Ph-mTO could not distinguish the G-quadruplex DNA structure from other nucleic acid, which probably owing to the pocket size and shape of the G-quadruplex DNA only could hinder the rotation of TPE moiety. Then the speculation was verified by molecular docking, TPE-mTO could adopt an appropriate pose in 3′ and 5′ binding pockets of CM22 (G-quadruplex), the multiple interaction between TPE-mTO and CM22 do hinder the rotation of TPE moiety and lead to strong fluorescence. In addition, the detection limit (DL) of TPE-mTO towards CM22 (prefolded G-quadruplex) was found as low as 4.1 nM. With the help of TPE-mTO , the G-quadruplex DNA structures in mitochondrion can be easily and quickly tracked without further washing operations. Overall, the probe we developed (TPE-mTO) was a simple but powerful tool for studying G-quadruplex structures. [ABSTRACT FROM AUTHOR]