16 results on '"Yanglin Ding"'
Search Results
2. Protein kinases in plant responses to drought, salt, and cold stress
- Author
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Shuhua Yang, Zhizhong Gong, Yan Guo, Yanglin Ding, Xuexue Chen, Baoshan Wang, Chun-Peng Song, and Yongqing Yang
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0106 biological sciences ,0301 basic medicine ,MAPK/ERK pathway ,Abiotic component ,Kinase ,fungi ,food and beverages ,Plant Science ,Biology ,MAPK cascade ,01 natural sciences ,Biochemistry ,General Biochemistry, Genetics and Molecular Biology ,Droughts ,Cell biology ,03 medical and health sciences ,Transduction (genetics) ,Focal Adhesion Kinase 2 ,030104 developmental biology ,Gene Expression Regulation, Plant ,Signal transduction ,Protein kinase A ,Cold stress ,Signal Transduction ,010606 plant biology & botany - Abstract
Protein kinases are major players in various signal transduction pathways. Understanding the molecular mechanisms behind plant responses to biotic and abiotic stresses has become critical for developing and breeding climate-resilient crops. In this review, we summarize recent progress on understanding plant drought, salt, and cold stress responses, with a focus on signal perception and transduction by different protein kinases, especially sucrose nonfermenting1 (SNF1)-related protein kinases (SnRKs), mitogen-activated protein kinase (MAPK) cascades, calcium-dependent protein kinases (CDPKs/CPKs), and receptor-like kinases (RLKs). We also discuss future challenges in these research fields.
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- 2021
3. RAF22, ABI1 and OST1 form a dynamic interactive network that optimizes plant growth and responses to drought stress in Arabidopsis
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Zhihui Sun, Zhenkai Feng, Yanglin Ding, Yuanpeng Qi, Shan Jiang, Zhen Li, Yu Wang, Junsheng Qi, Chunpeng Song, Shuhua Yang, and Zhizhong Gong
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Arabidopsis Proteins ,Gene Expression Regulation, Plant ,Mutation ,Arabidopsis ,Phosphoprotein Phosphatases ,Plant Science ,Molecular Biology ,Protein Kinases ,Abscisic Acid ,Droughts - Abstract
Plants adapt to their ever-changing environment via positive and negative signals induced by environmental stimuli. Drought stress, for instance, induces accumulation of the plant hormone abscisic acid (ABA), triggering ABA signal transduction. However, the molecular mechanisms for switching between plant growth promotion and stress response remain poorly understood. Here we report that RAF (rapidly accelerated fibrosarcoma)-LIKE MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 22 (RAF22) in Arabidopsis thaliana physically interacts with ABA INSENSITIVE 1 (ABI1) and phosphorylates ABI1 at Ser416 residue to enhance its phosphatase activity. Interestingly, ABI1 can also enhance the activity of RAF22 through dephosphorylation, reciprocally inhibiting ABA signaling and promoting the maintenance of plant growth under normal conditions. Under drought stress, however, the ABA-activated OPEN STOMATA1 (OST1) phosphorylates the Ser81 residue of RAF22 and inhibits its kinase activity, restraining its enhancement of ABI1 activity. Taken together, our study reveals that RAF22, ABI1, and OST1 form a dynamic regulatory network that plays crucial roles in optimizing plant growth and environmental adaptation under drought stress.
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- 2022
4. Plant abiotic stress response and nutrient use efficiency
- Author
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Liming Xiong, Yiting Shi, Yan Guo, Jijang Li, Yu Wang, Guohua Xu, Feng Qin, Peng Yun Wang, Jian-Kang Zhu, Dai-Yin Chao, Yongqing Yang, Shuhua Yang, Luis Herrera-Estrella, Huazhong Shi, Jingrui Li, Zhizhong Gong, and Yanglin Ding
- Subjects
0301 basic medicine ,Future studies ,Soil nutrients ,Plant Development ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Soil ,03 medical and health sciences ,0302 clinical medicine ,Nutrient ,Gene Expression Regulation, Plant ,Stress, Physiological ,Metals, Heavy ,Cellular ion homeostasis ,Phosphorylation ,Plant Proteins ,General Environmental Science ,Abiotic component ,Abiotic stress ,Ecology ,Stress signaling ,Plants ,030104 developmental biology ,030220 oncology & carcinogenesis ,Abiotic stress response ,Calcium Channels ,General Agricultural and Biological Sciences ,Signal Transduction ,Transcription Factors - Abstract
Abiotic stresses and soil nutrient limitations are major environmental conditions that reduce plant growth, productivity and quality. Plants have evolved mechanisms to perceive these environmental challenges, transmit the stress signals within cells as well as between cells and tissues, and make appropriate adjustments in their growth and development in order to survive and reproduce. In recent years, significant progress has been made on many fronts of the stress signaling research, particularly in understanding the downstream signaling events that culminate at the activation of stress- and nutrient limitation-responsive genes, cellular ion homeostasis, and growth adjustment. However, the revelation of the early events of stress signaling, particularly the identification of primary stress sensors, still lags behind. In this review, we summarize recent work on the genetic and molecular mechanisms of plant abiotic stress and nutrient limitation sensing and signaling and discuss new directions for future studies.
- Published
- 2020
5. The direct targets of CBFs: In cold stress response and beyond
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Hao Yang, Diyi Fu, Yue Song, Shuhua Yang, Xiaoyan Zhang, Yiting Shi, Zhizhong Gong, Jian Lv, Yanglin Ding, and Minze Li
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Arabidopsis ,Plant Science ,Biochemistry ,Regulon ,General Biochemistry, Genetics and Molecular Biology ,Deep sequencing ,Plant Growth Regulators ,Gene Expression Regulation, Plant ,Cold acclimation ,Arabidopsis thaliana ,Transcription factor ,Gene ,biology ,Arabidopsis Proteins ,Cold-Shock Response ,biology.organism_classification ,Lipid Metabolism ,Cell biology ,Trans-Activators ,Carbohydrate Metabolism ,Chromatin Immunoprecipitation Sequencing ,Chromatin immunoprecipitation ,circulatory and respiratory physiology ,Transcription Factors - Abstract
Cold acclimation in Arabidopsis thaliana triggers a significant transcriptional reprogramming altering the expression patterns of thousands of cold-responsive (COR) genes. Essential to this process is the C-repeat binding factor (CBF)-dependent pathway, involving the activity of AP2/ERF (APETALA2/ethylene-responsive factor)-type CBF transcription factors required for plant cold acclimation. In this study, we performed chromatin immunoprecipitation assays followed by deep sequencing (ChIP-seq) to determine the genome-wide binding sites of the CBF transcription factors. Cold-induced CBF proteins specifically bind to the conserved C-repeat (CRT)/dehydration-responsive elements (CRT/DRE; G/ACCGAC) of their target genes. A Gene Ontology enrichment analysis showed that 1,012 genes are targeted by all three CBFs. Combined with a transcriptional analysis of the cbf1,2,3 triple mutant, we define 146 CBF regulons as direct CBF targets. In addition, the CBF-target genes are significantly enriched in functions associated with hormone, light, and circadian rhythm signaling, suggesting that the CBFs act as key integrators of endogenous and external environmental cues. Our findings not only define the genome-wide binding patterns of the CBFs during the early cold response, but also provide insights into the role of the CBFs in regulating multiple biological processes of plants.
- Published
- 2021
6. The calcium transporter ANNEXIN1 mediates cold-induced calcium signaling and freezing tolerance in plants
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Zhizhong Gong, Yiting Shi, Yanglin Ding, Julia M. Davies, Marc R. Knight, Chun-Peng Song, Katie A. Wilkins, Shuhua Yang, Liang Ma, Yi Wang, Qiangbo Liu, Yan Guo, Heather Knight, Wang, Yi [0000-0002-3660-5859], Wilkins, Katie A [0000-0001-6513-856X], Yang, Shuhua [0000-0003-1229-7166], and Apollo - University of Cambridge Repository
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Mutant ,Arabidopsis ,chemistry.chemical_element ,Biology ,Calcium ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Gene Expression Regulation, Plant ,calcium-permeable transporter AtANN1 ,Freezing ,Arabidopsis thaliana ,Calcium Signaling ,Molecular Biology ,030304 developmental biology ,Calcium signaling ,0303 health sciences ,General Immunology and Microbiology ,Arabidopsis Proteins ,General Neuroscience ,Cold-Shock Response ,Cell Membrane ,calcium signal ,Transporter ,Articles ,biology.organism_classification ,freezing tolerance ,Cell biology ,Cold Temperature ,chemistry ,Phosphorylation ,OST1 kinase ,Protein Kinases ,030217 neurology & neurosurgery ,Transcription Factors - Abstract
The transient elevation of cytosolic free calcium concentration ([Ca(2+)](cyt)) induced by cold stress is a well‐established phenomenon; however, the underlying mechanism remains elusive. Here, we report that the Ca(2+)‐permeable transporter ANNEXIN1 (AtANN1) mediates cold‐triggered Ca(2+) influx and freezing tolerance in Arabidopsis thaliana. The loss of function of AtANN1 substantially impaired freezing tolerance, reducing the cold‐induced [Ca(2+)](cyt) increase and upregulation of the cold‐responsive CBF and COR genes. Further analysis showed that the OST1/SnRK2.6 kinase interacted with and phosphorylated AtANN1, which consequently enhanced its Ca(2+) transport activity, thereby potentiating Ca(2+) signaling. Consistent with these results and freezing sensitivity of ost1 mutants, the cold‐induced [Ca(2+)](cyt) elevation in the ost1‐3 mutant was reduced. Genetic analysis indicated that AtANN1 acts downstream of OST1 in responses to cold stress. Our data thus uncover a cascade linking OST1‐AtANN1 to cold‐induced Ca(2+) signal generation, which activates the cold response and consequently enhances freezing tolerance in Arabidopsis.
- Published
- 2020
7. Molecular Regulation of Plant Responses to Environmental Temperatures
- Author
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Yiting Shi, Yanglin Ding, and Shuhua Yang
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0106 biological sciences ,0301 basic medicine ,Plant growth ,Acclimatization ,Climate Change ,Plant Science ,Flowers ,Biology ,01 natural sciences ,03 medical and health sciences ,Gene Expression Regulation, Plant ,Stress, Physiological ,Cold acclimation ,Temperate climate ,Morphogenesis ,Molecular Biology ,Plant Proteins ,fungi ,Global warming ,Temperature ,food and beverages ,Vernalization ,Plants ,Heat stress ,Horticulture ,030104 developmental biology ,Germination ,Freezing stress ,Seeds ,010606 plant biology & botany - Abstract
Temperature is a key factor governing the growth and development, distribution, and seasonal behavior of plants. The entire plant life cycle is affected by environmental temperatures. Plants grow rapidly and exhibit specific changes in morphology under mild average temperature conditions, a response termed thermomorphogenesis. When exposed to chilling or moist chilling low temperatures, flowering or seed germination is accelerated in some plant species; these processes are known as vernalization and cold stratification, respectively. Interestingly, once many temperate plants are exposed to chilling temperatures for some time, they can acquire the ability to resist freezing stress, a process termed cold acclimation. In the face of global climate change, heat stress has emerged as a frequent challenge, which adversely affects plant growth and development. In this review, we summarize and discuss recent progress in dissecting the molecular mechanisms regulating plant thermomorphogenesis, vernalization, and responses to extreme temperatures. We also discuss the remaining issues that are crucial for understanding the interactions between plants and temperature.
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- 2019
8. Molecular Regulation of CBF Signaling in Cold Acclimation
- Author
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Yanglin Ding, Shuhua Yang, and Yiting Shi
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0106 biological sciences ,0301 basic medicine ,Plant growth ,Arabidopsis Proteins ,Mechanism (biology) ,Kinase ,Arabidopsis ,food and beverages ,Plant Science ,Biology ,01 natural sciences ,Cell biology ,03 medical and health sciences ,030104 developmental biology ,Gene Expression Regulation, Plant ,Gene expression ,Cold acclimation ,Signal transduction ,Transcription factor ,Function (biology) ,Signal Transduction ,Transcription Factors ,010606 plant biology & botany - Abstract
Cold stress restricts plant growth, development, and distribution. Understanding how plants transduce and respond to cold signals has long been a topic of interest. Traditional genetic and molecular analyses have identified C-repeat/DREB binding factors (CBFs) as key transcription factors that function in cold acclimation. Recent studies revealed the involvement of pivotal protein kinases and transcription factors in CBF-dependent signaling, expanding our knowledge of cold signal transduction from perception to downstream gene expression events. In this review, we summarize recent advances in our understanding of the molecular regulation of these core components of the CBF cold signaling pathway. Knowledge of the mechanism underlying the ability of plants to survive freezing temperatures will facilitate the development of crop plants with increased freezing tolerance.
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- 2018
9. EGR2 phosphatase regulates OST1 kinase activity and freezing tolerance in Arabidopsis
- Author
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Chun-Peng Song, Junping Gao, Yiting Shi, Jian Hua, Shuhua Yang, Zhizhong Gong, Yanglin Ding, and Jian Lv
- Subjects
Acclimatization ,Phosphatase ,Arabidopsis ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Fatty Acids, Monounsaturated ,03 medical and health sciences ,0302 clinical medicine ,Gene Expression Regulation, Plant ,Freezing ,Kinase activity ,Phosphorylation ,Protein kinase A ,Molecular Biology ,Freezing tolerance ,030304 developmental biology ,Myristoylation ,0303 health sciences ,General Immunology and Microbiology ,Arabidopsis Proteins ,General Neuroscience ,Cold-Shock Response ,Cell Membrane ,Articles ,biology.organism_classification ,Plants, Genetically Modified ,Cell biology ,Cold Temperature ,Enzyme Activation ,Protein Phosphatase 2C ,Binding ability ,Protein Kinases ,Protein Processing, Post-Translational ,030217 neurology & neurosurgery ,Function (biology) ,Signal Transduction - Abstract
OST1 (open stomata 1) protein kinase plays a central role in regulating freezing tolerance in Arabidopsis ; however, the mechanism underlying cold activation of OST1 remains unknown. Here, we report that a plasma membrane‐localized clade‐E growth‐regulating 2 (EGR2) phosphatase interacts with OST1 and inhibits OST1 activity under normal conditions. EGR2 is N‐myristoylated by N‐myristoyltransferase NMT1 at 22°C, which is important for its interaction with OST1. Moreover, myristoylation of EGR2 is required for its function in plant freezing tolerance. Under cold stress, the interaction of EGR2 and NMT1 is attenuated, leading to the suppression of EGR2 myristoylation in plants. Plant newly synthesized unmyristoylated EGR2 has decreased binding ability to OST1 and also interferes with the EGR2‐OST1 interaction under cold stress. Consequently, the EGR2‐mediated inhibition of OST1 activity is released. Consistently, mutations of EGRs cause plant tolerance to freezing, whereas overexpression of EGR2 exhibits decreased freezing tolerance. This study thus unravels a molecular mechanism underlying cold activation of OST1 by membrane‐localized EGR2 and suggests that a myristoyl switch on EGR2 helps plants to adapt to cold stress.
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- 2018
10. PUB25 and PUB26 Promote Plant Freezing Tolerance by Degrading the Cold Signaling Negative Regulator MYB15
- Author
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Yiting Shi, Jianmin Zhou, Jian Hua, Shuhua Yang, Zhuoyang Li, Zhizhong Gong, Yanglin Ding, Jinlong Wang, and Xi Wang
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Ubiquitin-Protein Ligases ,Arabidopsis ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Ubiquitin ,Gene Expression Regulation, Plant ,Cold acclimation ,Arabidopsis thaliana ,Threonine ,Molecular Biology ,030304 developmental biology ,0303 health sciences ,biology ,Arabidopsis Proteins ,Cell Biology ,biology.organism_classification ,Cell biology ,Cold Temperature ,biology.protein ,Phosphorylation ,Signal transduction ,030217 neurology & neurosurgery ,Homeostasis ,Signal Transduction ,Transcription Factors ,Developmental Biology - Abstract
Cold stress adversely affects plant growth, development, and crop productivity and quality. Plants employ cold acclimation strategy to protect them from cold damage. The transcription-factor-CBF-dependent cold signaling pathway plays a key role in plant cold acclimation. However, how this signaling pathway is dynamically and precisely regulated remains unknown. Here, we report that two U-box type E3 ubiquitin ligases, PUB25 and PUB26, positively regulate freezing tolerance in Arabidopsis thaliana. Both PUB25 and PUB26 poly-ubiquitinate MYB15, a transcriptional repressor of the CBF-dependent cold signaling pathway, leading to MYB15 degradation and thus enhanced CBF expression under cold stress. Furthermore, cold-activated OST1 specifically phosphorylates PUB25 and PUB26 at conserved threonine residues, enhancing their E3 activity and facilitating the cold-induced degradation of MYB15. Our results thus unravel the regulatory role of the OST1-PUB25/26 module in regulating the duration and amplitude of the cold response by controlling the homeostasis of the negative regulator MYB15.
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- 2019
11. OST1 Kinase Modulates Freezing Tolerance by Enhancing ICE1 Stability in Arabidopsis
- Author
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Qi Xie, Zhizhong Gong, Yanglin Ding, Hui Li, Shuhua Yang, and Xiaoyan Zhang
- Subjects
Arabidopsis ,Biology ,Genes, Plant ,General Biochemistry, Genetics and Molecular Biology ,Gene Expression Regulation, Plant ,Freezing ,Protein kinase A ,Molecular Biology ,Transcription factor ,Regulation of gene expression ,Arabidopsis Proteins ,Kinase ,Cell Biology ,Plants, Genetically Modified ,biology.organism_classification ,Cell biology ,Ubiquitin ligase ,Biochemistry ,biology.protein ,Phosphorylation ,Signal transduction ,Protein Kinases ,Signal Transduction ,Transcription Factors ,Developmental Biology - Abstract
SummaryCold stress is a major environmental factor that limits plant growth and development. The C-repeat-binding factor (CBF)-dependent cold signaling pathway is extensively studied in Arabidopsis; however, the specific protein kinases involved in this pathway remain elusive. Here we report that OST1 (OPEN STOMATA 1), a well-known Ser/Thr protein kinase in ABA signaling, acts upstream of CBFs to positively regulate freezing tolerance. The ost1 mutants show freezing hypersensitivity, whereas transgenic plants overexpressing OST1 exhibit enhanced freezing tolerance. The OST1 kinase is activated by cold stress. Moreover, OST1 interacts with both the transcription factor ICE1 and the E3 ligase HOS1 in the CBF pathway. Cold-activated OST1 phosphorylates ICE1 and enhances its stability and transcriptional activity. Meanwhile, OST1 interferes with the interaction between HOS1 and ICE1, thus suppressing HOS1-mediated ICE1 degradation under cold stress. Our results thus uncover the unexpected roles of OST1 in modulating CBF-dependent cold signaling in Arabidopsis.
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- 2015
12. Natural variation in CTB4a enhances rice adaptation to cold habitats
- Author
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Hongliang Zhang, Shiquan Shen, Haifeng Guo, Shuhua Yang, Lei Zhou, Zhanying Zhang, Jianping Yu, Luyuan Dai, Liang Ma, Yanglin Ding, Weiwei Zheng, Yawen Zeng, Gangling Li, Zichao Li, Jinjie Li, Jilong Li, Hong-li Shi, Xingming Sun, Yan Guo, Shuming Yang, and Yinghua Pan
- Subjects
0106 biological sciences ,0301 basic medicine ,Science ,Plant genetics ,Quantitative Trait Loci ,General Physics and Astronomy ,Quantitative trait locus ,Genes, Plant ,01 natural sciences ,Acclimatization ,General Biochemistry, Genetics and Molecular Biology ,Japonica ,Article ,Chromosomes, Plant ,03 medical and health sciences ,Gene Expression Regulation, Plant ,Stress, Physiological ,Genetic variation ,Botany ,Promoter Regions, Genetic ,Gene ,Ecosystem ,Phylogeny ,Multidisciplinary ,biology ,ATP synthase ,food and beverages ,Chromosome Mapping ,Genetic Variation ,Oryza ,General Chemistry ,biology.organism_classification ,Adaptation, Physiological ,Cold Temperature ,030104 developmental biology ,Haplotypes ,biology.protein ,Adaptation ,010606 plant biology & botany - Abstract
Low temperature is a major factor limiting rice productivity and geographical distribution. Improved cold tolerance and expanded cultivation to high-altitude or high-latitude regions would help meet growing rice demand. Here we explored a QTL for cold tolerance and cloned the gene, CTB4a (cold tolerance at booting stage), encoding a conserved leucine-rich repeat receptor-like kinase. We show that different CTB4a alleles confer distinct levels of cold tolerance and selection for variation in the CTB4a promoter region has occurred on the basis of environmental temperature. The newly generated cold-tolerant haplotype Tej-Hap-KMXBG was retained by artificial selection during temperate japonica evolution in cold habitats for low-temperature acclimation. Moreover, CTB4a interacts with AtpB, a beta subunit of ATP synthase. Upregulation of CTB4a correlates with increased ATP synthase activity, ATP content, enhanced seed setting and improved yield under cold stress conditions. These findings suggest strategies to improve cold tolerance in crop plants., Low temperature is a major factor limiting productivity in rice. Here the authors show that the CTB4a gene confers cold tolerance to japonica varieties adapted to cold habitats at the booting stage of development, and propose that CTB4a acts via an interaction with the beta subunit of ATP synthase.
- Published
- 2017
13. BRASSINOSTEROID-INSENSITIVE2 Negatively Regulates the Stability of Transcription Factor ICE1 in Response to Cold Stress in Arabidopsis
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Shuhua Yang, Keyi Ye, Hui Li, Chun-Peng Song, Yiting Shi, Zhizhong Gong, and Yanglin Ding
- Subjects
0106 biological sciences ,0301 basic medicine ,Arabidopsis ,Plant Science ,Biology ,Genes, Plant ,01 natural sciences ,03 medical and health sciences ,Gene Expression Regulation, Plant ,Brassinosteroids ,Freezing ,Cold acclimation ,Arabidopsis thaliana ,Phosphorylation ,Kinase activity ,Transcription factor ,Research Articles ,Arabidopsis Proteins ,Cold-Shock Response ,Intracellular Signaling Peptides and Proteins ,Nuclear Proteins ,Cell Biology ,Plants, Genetically Modified ,biology.organism_classification ,Cell biology ,Ubiquitin ligase ,Cold shock response ,030104 developmental biology ,biology.protein ,Signal transduction ,Protein Kinases ,Signal Transduction ,Transcription Factors ,010606 plant biology & botany - Abstract
Cold acclimation is a crucial strategy for plant survival at freezing temperatures. C-REPEAT BINDING FACTOR (CBF) genes are rapidly and transiently induced by low temperature and play important roles in cold acclimation. However, the mechanism underlying the attenuation of CBF expression during the later stages of the cold stress response is obscure. Here, we show that the protein kinase BRASSINOSTEROID-INSENSITIVE2 (BIN2) interacts with and phosphorylates INDUCER OF CBF EXPRESSION1 (ICE1) in Arabidopsis (Arabidopsis thaliana) under prolonged cold stress, facilitating the interaction between ICE1 and the E3 ubiquitin ligase HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE1 and thereby promoting ICE1 degradation. The kinase activity of BIN2 is inhibited during the early stages of the cold stress response and is subsequently restored, suggesting that BIN2 mainly downregulates ICE1 abundance when CBF expression is attenuated. A loss-of-function mutation of ICE1 partially suppresses the cold-induced expression of CBFs and compromises the enhanced freezing tolerance of bin2-3 bil1 bil2. These findings reveal an important role for BIN2 in fine-tuning CBF expression, and thus in balancing plant growth and the cold stress response.
- Published
- 2019
14. ESCRT-I Component VPS23A Affects ABA Signaling by Recognizing ABA Receptors for Endosomal Degradation
- Author
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Pedro L. Rodriguez, Feifei Yu, Qi Xie, Yanglin Ding, Yaorong Wu, Qingliang Li, Xiaoqiang Cao, Miaomiao Tian, Borja Belda-Palazón, Lijuan Lou, and Shuhua Yang
- Subjects
0106 biological sciences ,0301 basic medicine ,ESCRTs ,Endosome ,Arabidopsis ,Receptors, Cell Surface ,Plant Science ,ABA receptors ,Bioinformatics ,01 natural sciences ,ESCRT ,03 medical and health sciences ,chemistry.chemical_compound ,Downregulation and upregulation ,Ubiquitin ,Plant Growth Regulators ,Gene Expression Regulation, Plant ,BIOQUIMICA Y BIOLOGIA MOLECULAR ,Receptor ,Molecular Biology ,Abscisic acid ,Pyr1 ,biology ,Endosomal Sorting Complexes Required for Transport ,Arabidopsis Proteins ,fungi ,food and beverages ,biology.organism_classification ,Cell biology ,Endosomal trafficking ,030104 developmental biology ,chemistry ,biology.protein ,Plant hormone ,010606 plant biology & botany ,Abscisic Acid ,Protein Binding ,Signal Transduction - Abstract
[EN] Recent discovery of PYR/PYL/RCAR-type abscisic acid (ABA) receptors has become one of most significant advances in plant science in the past decade. In mammals, endosomal sorting acts as an important pathway to downregulate different types of receptors, but its role in plant hormone signaling is poorly understood. Here, we report that an ubiquitin E2-like protein, VPS23A, which is a key component of ESCRT-I, negatively regulates ABA signaling. VPS23A has epistatic relationship with PYR/PYL/RCAR-type ABA receptors and disruption of VPS23A enhanced the activity of key kinase OST1 in the ABA signaling pathway under ABA treatment. Moreover, VPS23A interacts with PYR1/PYLs and K63-linked diubiquitin, and PYL4 possesses K63-linked ubiquitinated modification in vivo. Further analysis revealed that VPS23A affects the subcellular localization of PYR1 and the stability of PYL4. Taken together, our results suggest that VPS23A affects PYR1/PYL4 via vacuole-mediated degradation, providing an advanced understanding of both the turnover of ABA receptors and ESCRTs in plant hormone signaling., This research was supported by grant 2016YFA0500500 from the National Basic Research Program of China and NSFC 31571441 from the National Science Foundation of China.
- Published
- 2016
15. The cbfs triple mutants reveal the essential functions of CBFs in cold acclimation and allow the definition of CBF regulons in Arabidopsis
- Author
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Yuxin, Jia, Yanglin, Ding, Yiting, Shi, Xiaoyan, Zhang, Zhizhong, Gong, and Shuhua, Yang
- Subjects
Cold Temperature ,Base Sequence ,Arabidopsis Proteins ,Gene Expression Regulation, Plant ,Sequence Analysis, RNA ,Stress, Physiological ,Acclimatization ,Freezing ,Mutation ,Arabidopsis ,Genes, Plant ,Transcriptome ,Regulon - Abstract
In Arabidopsis, the C-repeat binding factors (CBFs) have been extensively studied as key transcription factors in the cold stress response. However, their exact functions in the cold response remains unclear due to the lack of a null cbf triple mutant. In this study, we used CRISPR/Cas9 technology to mutate CBF1 or CBF1/CBF2 in a cbf3 T-DNA insertion mutant to generate cbf1,3 double and cbf1 cbf2 cbf3 (cbfs) triple mutants. The response of the cbfs triple mutants to chilling stress is impaired. Furthermore, no significant difference in freezing tolerance was observed between the wild-type and the cbf1,3 and cbfs mutants without cold acclimation. However, the cbfs mutants were extremely sensitive to freezing stress after cold acclimation, and freezing sensitivity ranking was cbfs cbf1,3 cbf3. RNA-Seq analysis showed that 134 genes were CBF regulated, of which 112 were regulated positively and 22 negatively by CBFs. Our study reveals the essential functions of CBFs in chilling stress response and cold acclimation, as well as defines a set of genes as CBF regulon. It also provides materials for the genetic dissection of components in CBF-dependent cold signaling.
- Published
- 2016
16. Temperature-dependent autoimmunity mediated by chs1 requires its neighboring TNL gene SOC3
- Author
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Yuancong Wang, Yao Zhang, Shanshan Wang, Yule Liu, Yanglin Ding, Jingyan Liu, Xiaoyan Zhang, and Shuhua Yang
- Subjects
0106 biological sciences ,0301 basic medicine ,Physiology ,Mutant ,Arabidopsis ,Down-Regulation ,Autoimmunity ,Plant Science ,Plasma protein binding ,medicine.disease_cause ,Genes, Plant ,01 natural sciences ,Models, Biological ,03 medical and health sciences ,Suppression, Genetic ,Downregulation and upregulation ,Gene Expression Regulation, Plant ,Loss of Function Mutation ,medicine ,Amino Acid Sequence ,Binding site ,Cloning, Molecular ,Receptors, Immunologic ,Genes, Suppressor ,Gene ,Genetics ,Mutation ,biology ,Base Sequence ,Arabidopsis Proteins ,Temperature ,biology.organism_classification ,Phenotype ,030104 developmental biology ,Gain of Function Mutation ,010606 plant biology & botany ,Protein Binding ,Subcellular Fractions - Abstract
Toll/interleukin receptor (TIR)-nucleotide binding site (NB)-type (TN) proteins are encoded by a family of 21 genes in the Arabidopsis genome. Previous studies have shown that a mutation in the TN gene CHS1 activates the activation of defense responses at low temperatures. However, the underlying molecular mechanism remains unknown. To genetically dissect chs1-mediated signaling, we isolated genetic suppressors of chs1-2 (soc). Several independent soc mutants carried mutations in the same TIR-NB-leucine-rich repeat (LRR) (TNL)-encoding gene SOC3, which is adjacent to CHS1 on chromosome 1. Expression of SOC3 was upregulated in the chs1-2 mutant. Mutations in six soc3 alleles and downregulation of SOC3 by an artificial microRNA construct fully rescued the chilling sensitivity and defense defects of chs1-2. Biochemical studies showed that CHS1 interacted with the NB and LRR domains of SOC3; however, mutated chs1 interacted with the TIR, NB and LRR domains of SOC3 in vitro and in vivo. This study reveals that the TN protein CHS1 interacts with the TNL protein SOC3 to modulate temperature-dependent autoimmunity.
- Published
- 2016
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