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Proteomic profiling of brain cell types using isolation-based strategies pose limitations in resolving cellular phenotypes representative of their native state. We describe a mouse line for cell type-specific expression of biotin ligase TurboID, for in vivo biotinylation of proteins. Using adenoviral and transgenic approaches to label neurons, we show robust protein biotinylation in neuronal soma and axons throughout the brain, allowing quantitation of over 2000 neuron-derived proteins spanning synaptic proteins, transporters, ion channels and disease-relevant druggable targets. Next, we contrast Camk2a-neuron and Aldh1l1-astrocyte proteomes and identify brain region-specific proteomic differences within both cell types, some of which might potentially underlie the selective vulnerability to neurological diseases. Leveraging the cellular specificity of proteomic labeling, we apply an antibody-based approach to uncover differences in neuron and astrocyte-derived signaling phospho-proteins and cytokines. This approach will facilitate the characterization of cell-type specific proteomes in a diverse number of tissues under both physiological and pathological states.

Multifunctional enzymatic nanomaterials (nanozymes) with good biocompatibility, strong enzymatic activity, and substrate specificity are the desired alternatives to natural enzymes. Here, the design of a peroxidase mimetic nanozyme capable of detecting and depleting glutathione (GSH) in the bacterial cells is presented. The nanozyme palladium-iron nanostructure decorated graphdiyne nanosheet (PdFe/GDY) was prepared by a facile hydrothermal approach. It can efficiently catalyze the decomposition of H2O2 to produce •OH by a peroxidase-like mechanism. Surprisingly, the PdFe/GDY nanozymes can detect GSH in bacteria with excellent specificity and ultra-low detection limit of 24.45 nM. Moreover, the PdFe/GDY nanozymes can consume GSH for bacterial disinfection with killing efficacy > 99.99%, promote wound healing and do not cause significant toxicity in vitro and in vivo. To our knowledge, this is the first example of a GDY-based nanozyme biosensor and antibacterial agent. Collectively, this work would be indicative to construct GDY-based nanozymes in clinical diagnosis and sterilization.

Dendritic Pt–Cu nanoparticles were synthesized by a facile one-step method with the help of surfactant Brij58 at room temperature, and we also studied the effects of different Pt–Cu ratios on the morphology and size of nanoparticles. In addition, we further tuned the morphology of the Pt–Cu nanostructures by introducing bromide ions, eventually leading to the appearance of some tripod-like structures. Compared with dendritic Pt–Cu and commercial Pt black, these tripod-like Pt–Cu nanostructures exhibited higher electrocatalytic activity and CO tolerance for catalyzing methanol oxidation.

Fe3O4 nanoparticles were synthesized on graphene oxide (GO), then mixed with polyethylenimine to obtain GO/Fe3O4/PEI nanocomposites. Due to the high surface area of GO, superparamagnetism of Fe3O4, and excellent complex ability of PEI, the nanocomposites showed extremely high Cu2+ removal efficiency. The Cu2+ removal capacity was 157 mg g−1, which is higher than most reported results. The adsorption kinetics could be best described by a pseudo-second-order model. Moreover, GO/Fe3O4/PEI nanocomposites could be easily recycled by magnetic separation. After five cycles, the removal efficiency remained 84%.

Colloidal platinum–ruthenium bimetallic nanoparticles with 2.3 nm were synthesized in solution and then immobilized on tin dioxide powder to make a supported nanocatalyst (Pt–Ru/SnO 2 ). The immobilized bimetallic nanoparticles had the same size and size distribution as the solution dispersed ones. Selective hydrogenation of o -chloronitrobenzene to o -chloroaniline was performed over the as-prepared Pt–Ru/SnO 2 nanocatalyst without using any solvent; 99.5% selectivity to o -chloroaniline at ∼100% conversion was achieved.

Crystal structure of the title compound, [Ni(Im)6] (NO3)2·4H2O (Im = imidazole), was determined by X-ray crystallographic analysis. The crystal structure consists of discrete Ni(Im)26+ cation, NO−3 anion and four uncoordinated water molecules. It crystallizes in the hexagonal system, space group P63, with lattice parameters a = b = 0.9003(2) nm, c = 2.1034(4) nm, and Z = 2. The Ni(II) ion is centro- symmetric octahedron geometry with the NiN6 core. Six imidazole molecules are coordinated to each nickel (II) atom through its tertiary nitrogen atom. The short and long bond distances of Ni-N are 0.2059(6) and 0.2204(7) nm, respectively. In the solid state, [Ni(Im)6]2+, H2O moieties and nitrate anions form the three dimensional hydrogen bonds network which stabitizes the crystal structure.

The structures of [MCl2(C6H4B3CH2COPh)2] [M=Zn(II) (1), Co(II) (2)] have been determined by X-ray crystallography. They were characterized by elemental analysis, IR spectrum, electronic spectrum and thermogravimetric-differential thermal analysis. They all crystallize in the triclinic system, space pup Pi, with lattice parameters a=0.9449(2) nm, b=1.1291(2) nm, c=1.3637(3) nm, α=111.70(3)°, β= 94.33(3)°, y=90.97(3), Z=2 for compound 1; a=0.9437(2) nm, b= 1.1277(2) nm, c= 1.3650(3) nm, a=111.76(3)°, β=94.50(3)°, y=90.80(3)°, Z=2 for compound 2. The metal ions are all coordinated by two C1−- anions and two nitrogen atoms of 1-benzoylmethyl-bentriazole ligands. forming the distorted tetrahedral geometry. The Zn—Cl and Zn—N bond lengths are 0.2209(2), 0.2210(2) nm and 0.2059(4), 0.2067(4) nm. respectively. The CO-Cl 3 and Co–N bond lengths are 0.2215(2). 0.2222(2) nm and 0.2028(5), 0.2045(5) nm. respectively. The thermogravimetric (TG) data indicate that they are nearly similar in TG curve, and there are not structural transitions in the two compounds. They all have a high thd stability. But, there is little difference in DTG (differential thermogravimetric) curves of those two compounds. Elemental analysis, electronic and IR spectra are in agreement with the structural data.

In this paper, the Mo–V Keggin compound 1, KNa6[VMo12O40]2(OH)(H2O)36, had obtained. Single crystal X-ray analysis revealed that there is the nanometer-sized water cube, Na6(KOH)(H2O)36, in [VMo12O40]3− Keggin structure. The Na6(KOH)(H2O)36 forms one cuboid house with long side of 22.456 A and short side of 11.228 A. The cation water cube acts as a “host” and the globular anion [VMo12O40]3− as “guest” which was captured. The cycle voltammetry study showed that above compound had excellent electrocatalytic activity toward the reduction of bromate. Thermogravimetric analysis was in agreement with the crystal data. In this paper, the Mo–V Keggin compound 1, KNa6[VMo12O40]2(OH)(H2O)36, had obtained. Single crystal X-ray analysis revealed that there is the nanometer-sized water cube, Na6(KOH)(H2O)36, in [VMo12O40]3− Keggin structure. The Na6(KOH)(H2O)36 forms one cuboid house with long side of 22.456 A and short side of 11.228 A. The cation water cube acts as a “host” and the globular anion [VMo12O40]3− as “guest” which was captured. The cycle voltammetry study showed that above compound had excellent electrocatalytic activity toward the reduction of bromate. Thermogravimetric analysis was in agreement with the crystal data.

Two new decavanadate metal complexes, [Co(H2O)6]2[H2V10O28]·6H2O (1) and (NH4)2[Ca(H2O)7]2[V10O28] (2), have been synthesized under hydrothermal condition by using chlorhydric acid as the initiator at 120 °C. The aqueous NaVO3 solution with an aqueous solution of CoCl2·6H2O were used for generating 1 and aqueous CaCl2·2H2O and NH4VO3 solution were employed for creating 2. Compound 1 consisted of discrete hexa-aqua-cobalt [Co(H2O)6]2+ cations, [H2V10O28]4− anions and non-coordination water molecules. Compound 2 were composed of hepta-aqua-calcium [Ca(H2O)7]2+ cations, ammonium NH4 + and [V10O28]6− anion. For compound 2, the distorted pentagonal bipyramid [Ca(H2O)7]2+ is uncommon. In the crystal lattice, hydrogen bonds played an important role on connecting cations, anions and non-coordinated water molecules to form the three-dimensional network.

Three new metal-containing ionic liquids (ILs) of N,N′-dialkylbenzimidazolium of [(CnH2n+1)2-bim]2[CuCl4] (n = 8, 10, 12) (bim = benzimidazole) were synthesized, and the structures were characterized by X-ray crystallography, IR spectroscopy, and elemental analysis. The cations of three complexes adopted U-shaped conformations and were packed in a bilayer fashion. The thermal behaviors of three complexes were studied by differential scanning calorimetry (DSC) and thermogravimetry (TG). The ionic conductivities measurements showed that the ionic conductivities of the three complexes were different in absolute ethanol, and the ionic conductivities may relate to the coordination environment found for Cu(II) of three complexes.

The title compound, 3-methyl-4-phenyl-5-(2-pyridyl)-1,2,4-triazole was synthesized by reaction of diphenylphosphazoanilide with N-acetyl-N′-(2-pyridoyl)hydrazine. Crystals suitable for X-ray analysis were obtained from a mixed solution of water and ethanol in a one-to-one volume ratio. The crystal is orthorhombic, space group P212121 with crystallographic parameters: a = 19.414(4) A, b = 17.172(3) A, c = 7.4850(15) A, β = 90.00°, μ = 0.079 mm−1, V = 2495.3(8) A3, Z = 8, Dc = 1.258 g/cm3, F(000) = 992, T = 295(2) K. The X-ray results showed that in the crystal structure of the title compound, the 1,2,4-triazole, pyridine and benzene rings are not coplanar. The title compound was synthesized by reaction of diphenylphosphazoanilide with N-acetyl-N′-(2-pyridoyl)hydrazine, and the crystal structure shows that its three aromatic rings (1,2,4-triazole, pyridine and benzene rings) do not share a common plane.

Four tetranuclear 2-mercaptobenzothiazole cluster compounds [M4O(MBT)6] (M = Fe, Co, Ni, Cu; MBT = 2-mercaptobenzhothiazole) were synthesized and characterized by elemental analyses, IR and UV–vis spectra, and single crystal X-ray crystallography. The crystal structures of [M4O(MBT)6] (M = Fe, Co, Ni) confirm that the four metal(II) atoms locate four capsheaves of a tetrahedral skeletal structure and a tetracoordinate O2− as an interstitial atom occupies the centre position of this tetrahedron. The metal atoms all possess slightly distorted tetrahedral geometry. The thermal gravimetry data indicate that these clusters all have good thermal stability. The nonlinear absorption of four cluster solutions (in DMF) were measured by open-aperture Z-scan technique at a 532 nm wavelength. The results of Z-scan experiments show that these clusters all have remarkable and very strong nonlinear optical absorption effects. The largest two-photon absorption cross-section is 172880 GM for cluster [Fe4O(MBT)6].

The crystal and molecular structures of the complexes of [NiCl2(N4-trzCH2CH2 COPh)4]⋅6H2O and [CoCl2(N4-trzCH2CH2COPh)4]⋅ 6H2O (trz = 1,2,4-triazole) have been determined by X-ray crystallography. They are isomorphous. The crystal structures are very similar. They both crystallize in the monoclinic system, space group P21/c, Z = 4; the former with lattice parameters a = 8.0391(2), b = 10.8215(2), c = 29.0133(2) A, β = 94.792(1)°; the latter with a = 8.039(2), b = 10.822(2), c = 29.013(6) A, β = 94.79(3)°. They all display almost perfect octahedral coordination geometry around the central metal atom. Each metal atom is coordinated by four N atoms of triazole from four β-propiophenone-1,2,4-triazole ligands and two chloride anions in a trans arrangement. In addition to the coordinating metal complex, there are six uncoordinated water molecules, which complete the crystal structure. In the solid state, the title two compounds form three dimensional network structures through hydrogen bonds. The intermolecular hydrogen bonds connect the [MCl2(C2H2N3CH2CH2COPh)4] and H2O moieties.

The crystal and molecular structure of [ZnCl2(N4-trzCH2COPh)2] (trz = 1,2,4-triazole) has been determined by X-ray crystallography. It crystallizes in the monoclinic system, space group C2/c, with lattice parameters a = 26.7892(4) A, b = 4.9392(2) A, c = 20.7872(1) A, β = 127.517 (1)○, and z = 4. The complex has C2 symmetry. The coordination geometry of each Zn atom is distorted tetrahedral formed by two Cl atoms and two tertiary N atoms of the α-(1,2,4-triazole-1-yl)-acetophenone ligands. The two equivalent Zn–Cl and Zn–N bonds form bond angles around the Zn(II) atom in the range 101.4(1)–120.8(1)○. C–H ⋅⋅⋅ O intermolecular interactions link the molecules in infinite chains in the [101] direction.

The crystal and molecular structures of the complex of [Ni{(iPrO)2dtp}2(py)2] and [Cd{(iPrO)2dtp}2(py)2] (dtp = dithiophosphate, py = pyridine) have been determined by X-ray crystallography. They are isomorphous. The crystal structures are very similar and consist of discrete molecules of [Ni{(iPrO)2dtp}2(py)2] and [Cd{(iPrO)2dtp}2(py)2], respectively. They both crystallize in the monoclinic system, space group P21/c, the former with lattice parameters a = 6.489(1) A, b = 14.830(3) A, c = 16.386(3) A, β = 99.74(3), and Z = 2; the latter with a = 6.461(3) A, b = 14.583(4) A, c = 17.433(4) A, β = 99.55(3)°, and Z = 2. They all display distorted octahedral geometry around the central metal atom. In the complexes, two O,O′-diisopropyl dithiophosphate ions act as bidentate ligands with their S atoms coordinated to metal. Each forms a four-membered chelate ring in the equatorial plane. The N atoms from two pyridine ligands are axially coordinated to the metal atom. The Ni–S bond distances are 2.5137(10) and 2.5386(9) A, and the Ni–N bond distances are 2.127(3) A. The Cd–S(1) and Cd–S(2) bond distance are 2.694(1) and 2.704(1) A, respectively, and the Cd–N bond distances are 2.399(3) A. The IR spectra data is in agreement with the structural data.

The title compound, C11H10N6S, was prepared by the reaction of ethyl 2-(1H-1,2,4-triazol-1-yl)­acetate with hydrazine and phenyl iso­thio­cyanate. The molecular structure and packing are stabilized by an N—H⋯N intermolecular hydrogen-bond and C—H⋯π interactions.

In the title compound, C12H20N4OS2, the dihedral angle between the plane of the N,N-di­methyl­di­thio­carbamate group and the plane of the triazole moiety is 9.96 (2)°. There are weak C—H⋯N intermolecular interactions in the crystal structure, contributing to its stability.

In the title compound, C17H17N5O2S, the dihedral angles between the plane of the 4,6-di­methyl-2-mercapto­pyrimidine group and the plane of the triazole and p-methoxy­phenyl­carbonyl groups are 79.64 (2) and 0.44 (2)°, respectively. There are weak C—H⋯O and C—H⋯N intermolecular interactions between the mol­ecules in the crystal lattice.

Supported Pt nanocatalysts with controlled particle size and size distribution were prepared by immobilizing Pt colloids on different iron oxide supports (γ-Fe2O3, Fe3O4, Fe2O3) via physical adsorption. These catalysts were used to catalyze the liquid phase hydrogenation of o-chloronitrobenzene (o-CNB) to o-chloroaniline (o-CAN) under mild reaction conditions (298 K, 0.1 MPa). The Pt/γ-Fe2O3 exhibited higher catalytic selectivity and activity than other similar works (selectivity = 99.9%, activity = 0.20 molo-CNB/(molPt s)). The stability of the Pt/γ-Fe2O3 was investigated, and the result showed that higher drying temperature could increase the stability of the catalyst.

In the title structure, C21H16BrClN2, the pyrazoline ring is approximately coplanar with the N-substituted phenyl ring. The dihedral angles formed by the pyrazoline ring with the 4-chlorophenyl and 4-bromophenyl rings are 78.82 and 10.9°, respectively.

The title compound, C13H9ClN2, was prepared by reaction of o-phenyl­enediamine with 4-chloro­benzaldehyde under reflux in ethanol. The mol­ecules are linked by N—H⋯N inter­actions.

The title compound, C18H18N2O, was prepared by the reaction of 3-phenyl-1-(p-tol­yl)prop-2-en-1-one with hydrazine hydrate in acetic acid. The pyrazole ring is in an envelope conformation. The crystal packing is stabilized by van der Waals forces.

The title compound, C16H14Cl2N2, was prepared by the reaction of hydrazine with 2-chloro­acetophenone at room temperature. The mol­ecule has C2 symmetry with the mid-point of the N—N bond lying on the twofold axis.

The title compound, C18H16N4O, was prepared by the reaction of 5-phenyl-1H-pyrazole-3-carbohydrazide with 4-methyl­benzaldehyde in refluxing ethanol. The structure exhibits weak inter­molecular N—H⋯O and N—H⋯N hydrogen-bonding inter­actions.

The title compound, C20H14Cl2N2, was prepared by reaction of o-phenyl­enediamine with 2-chloro­benzaldehyde under reflux in ethanol. The crystal structure is stabilized by van der Waals inter­actions.

The title compound, C12H13NO2S, was prepared by the reaction of acetylacetone with phenyl isothiocyanate. In addition to the intramolecular O—H...O hydrogen bond, there is a weak intermolecular N—H...S hydrogen bond, which links the molecules into chains extended along the c axis. The crystal packing is further stabilized by van der Waals forces.

In the title compound, [Co(C8H8NO2)2], the Co atom has a distorted square-planar coordination involving two N atoms and two O atoms from two ethanone 1-(2-hydroxyphenyl)oximato ligands. The molecular structure and packing are stabilized by intramolecular O—H...O hydrogen bonds and C—H...π intermolecular interaction.

In the title compound, [CuCl2(C6H5Cl2N)2], each Cu atom has a distorted tetra­hedral coordination involving two Cl− anions and two 2-chloro-5-(chloro­meth­yl)pyridine ligands. The mol­ecular structure and packing are stabilized by intra- and intermolecular C—H⋯Cl hydrogen-bonding inter­actions.

The title compound, [Pd(C14H12O2P)2]·CH2Cl2, consists of mononuclear [(Ph2PCH2COO)2Pd] complexes and dichloro­methane mol­ecules in a 1:1 molar ratio. The two (diphenyl­phosphino)acetate ligands are coordinated to the central Pd atom by their P and O atoms, forming five-membered chelate rings. The PdII atom has a distorted square-planar configuration [Pd—O = 2.076 (3) and 2.082 (3) A, and Pd—P = 2.214 (1) and 2.216 (1) A].

The title compound, C15H15N3S, was prepared by the reaction of acetophenone and hydrazine with phenyl­isothio­cyanate. In the mol­ecule, all bond lengths and angles are within normal ranges. The two phen­yl rings make a dihedral angle of 61.6 (2)°. The crystal packing is stabilized by van der Waals forces.

The title compound, [Cu2Cl2(C18H15O3P)4], is a centrosymmetric dinuclear complex, where each CuI atom has a distorted tetra­hedral coordination involving two triphenyl­phosphite ligands and two bridging Cl− anions. The mol­ecular structure and packing are stabilized by C—H⋯O and C—H⋯π inter­actions.

The title compound, C10H13N3S, was prepared by the reaction of acetone with hydrazine and phenyl iso­thio­cyanate. The molecular structure and crystal packing are stabilized by N—H⋯N and N—H⋯S hydrogen bonds.

The title compound, C15H13N3S, was prepared by the reaction of 1-(2-chloro­ethyl)­benzene with hydrazine and phenyl iso­thio­cyanate. Packing is stabilized by N—H⋯S intermolecular hydrogen bonds and C—H⋯π interactions.

In the title compound, [Co(NCS)2(C4H6N2)2], each cobalt(II) ion is coordinated by two N atoms from two 2-methyl-1H-imidazole ligands and two N atoms from two iso­thio­cyanate groups. The Co atom lies on a mirror plane and adopts a slightly distorted tetrahedral coordination. The molecular structure and packing are stabilized by intramolecular and intermolecular hydrogen-bond interactions.

In the title compound, [Cd(N3)2(C10H8N2)2], the cadmium(II) ion is coordinated by two N atoms from two azide groups and four N atoms from two 2,2′-bi­pyridine ligands. The coordin­ation geometry of the cadmium(II) ion is distorted octahedral. The packing is stabilized by intermolecular C—H⋯N hydrogen-bond interactions.

In the title compound, [Zn(N3)2(C10H8N2)2]·H2O, the zinc(II) ion is coordinated by two N atoms from two azide groups and four N atoms from two 2,2′-bi­pyridine ligands. The coordination geometry of the zinc(II) ion is slightly distorted octahedral. In the solid state, the title compound forms a network structure via hydrogen bonds.

In the title compound, C17H19ClN2O3, the dihedral angles formed by the butyl group and the imidazole ring with the benzene ring are 86.4 (4) and 88.9 (3)°, respectively. The dihedral angle between the imidazole ring and the butyl group is 77.1 (5)°. The structure is stabilized by C—H⋯O and C—H⋯π interactions.

In the title compound, C13H11N3OS2, the dihedral angles between the planes of the triazole and phenyl rings and that through the four NC3 atoms of the C=C are 76. (1) and 57.6 (1)°, respectively. There are some intermolec­ular interactions in the crystal structure.

In the title compound, [ZnCl2(C6H4N3CH2COPh)2], the zinc ion is coordinated by two Cl− anions and two N atoms of two triazole ligands, forming a distorted tetrahedral geometry. The molecular structure and packing are stabilized by van der Waals interactions.

In the crystal of the title compound, 2C(5)H(7)N(2) (+)·C(4)N(4)S(6) (2-)·H(2)O, inter-molecular N-H⋯S and N-H⋯N hydrogen bonds link four cations and two dianions into a centrosymmetric cluster. The crystal packing is further consolidated by π-π inter-actions between the five- and six-membered rings of neighbouring clusters [centroid-centroid distances = 3.692 (3), 3.718 (3), 3.660 (3) and 3.696 (3) Å] and via O-H⋯N, O-H⋯S and N-H⋯O hydrogen bonds involving the uncoordinated water mol-ecules.

The title compound, C17H16ClN5·C3H7NO, was prepared by reaction of p-toluidine with 2,4,6-trichloro-1,3,5-triazine at room temperature. The dihedral angles between the triazine ring and the pendant rings are 3.61 (12) and 53.11 (12)°. An intramolecular C—H...N interaction occurs. The packing is stabilized by N—H...N and N—H...O hydrogen bonds and C—H...π and π–π [centroid–centroid distance = 3.763 (1) Å] interactions.

In the title compound, C(16)H(16)N(2)O(3), the dihedral angle between the mean planes of the aromatic ring systems is 30.90 (15)°. In the crystal structure, the mol-ecules form [010] chains by way of N-H⋯N hydrogen bonds.

In the mol-ecule of the title compound, C(18)H(16)N(4)O, the intra-molecular N-H⋯N hydrogen bond results in the formation of a planar five-membered ring, which is also co-planar with the adjacent five-membered ring, being oriented at a dihedral angle of 1.23 (3)°. The dihedral angles formed by the planar pyrazole ring with the adjacent phenyl ring and the other phenyl ring are 7.29 and 11.21°, respectively. The dihedral angle between the two phenyl rings is 18.07°. In the crystal structure, inter-molecular N-H⋯O hydrogen bonds link the mol-ecules.

The title compound, C20H12Br2N2, was prepared by the reaction of 1-(3-bromophenyl)-2-(4-bromophenyl)ethane-1,2-dione with o-phenylenediamine in refluxing ethanol. In the molecule, all bond lengths and angles are within normal ranges. The dihedral angle between the two benzene rings is 34.89 (1)°. The dihedral angles between the benzene rings and the quinoxaline system are 57.23 (1) and 36.75 (1)°. The crystal packing is stabilized by van der Waals forces.

In the title compound, C24H17BrN2OS, the benzimidazole ring system is planar. The dihedral angles formed by the bromo­phenol, phenyl and thienyl rings with the benzimidazole ring system are 78.4 (1), 80.2 (2) and 33.1 (1)°, respectively. The mol­ecular structure and packing are stabilized by intra- and inter­molecular O—H⋯N and C—H⋯N hydrogen-bonding inter­actions and C—H⋯π inter­actions.

The title compound, C15H16N2S, was prepared by the reaction of 3,5-dimethyl­phenyl­amine with 1-isothio­cyanato­benzene. In the mol­ecule, all bond lengths and angles are within normal ranges. The dihedral angle between the two aromatic rings is 48.77 (1)°. The crystal packing is stabilized by van der Waals forces.