1. Immunological features and efficacy of the recombinant subunit vaccine LTB-EMY162 against Echinococcus multilocularis metacestode
- Author
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Feng Tang, Feng Lin, Yang Quanyu, Kunmei Liu, Runle Li, Le Guo, Wei Wang, and Ge Rili
- Subjects
0301 basic medicine ,Immunogen ,Lymphocyte ,T-Lymphocytes ,Cell ,Antibodies, Helminth ,Biology ,Echinococcus multilocularis ,Lymphocyte Activation ,Applied Microbiology and Biotechnology ,law.invention ,Microbiology ,03 medical and health sciences ,Interferon-gamma ,Mice ,0302 clinical medicine ,law ,medicine ,Animals ,Humans ,Life Cycle Stages ,Mice, Inbred BALB C ,Vaccines, Synthetic ,Cell growth ,General Medicine ,T lymphocyte ,biology.organism_classification ,Metacestode ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Antigens, Helminth ,Vaccines, Subunit ,Recombinant DNA ,Female ,Interleukin-4 ,Biotechnology - Abstract
Alveolar echinococcosis is a zoonotic disease caused by the infection of the larval stage Echinococcus multilocularis with worldwide distribution especially in the northwest China. It is important to develop a well-tolerated immunoprophylaxis against E. multilocularis for alveolar echinococcosis control. In this study, a prokaryotic expression system for recombinant immunogen LTB-EMY162 was established, and the immunological features, sensitized lymphocyte, IL-4/IFN-γ secreted, prophylactic effect, and therapeutic effect were also evaluated. Arctic Express (DE3) system, Ni2+-charged and molecular sieve chromatography were used to obtain a high-purity 29 kDa protein. The ELISA and lymphocyte proliferation assay showed that LTB-EMY162 induced high-titer specific IgG against EMY162 and E. multilocularis protoscoleces protein in BALB/c mice and promoted sensitized T lymphocyte cell proliferation, and LTB-EMY162 stimulated Th cell to secrete IL-4 and IFN-γ and induced a Th1/Th2 mixed type immunological response. We also found that LTB-EMY162 significantly inhibited the cysts formation by challenging with 1000 E. multilocularis protoscoleces. The growth of protoscoleces and cysts were also significantly decreased by treating with LTB-EMY162 in 1000 protoscoleces intraperitoneal injection therapeutic mice model. In conclusion, we have constructed a subunit vaccine LTB-EMY162 which has prevention and therapeutic effect against E. multilocularis infection.
- Published
- 2017